Changes In Dynamic Properties Research Articles

Resonant acoustic rheometry for non-contact characterization of viscoelastic biomaterials

Resonant Acoustic Rheometry (RAR) is a new, non-contact technique to characterize the mechanical properties of soft and viscoelastic biomaterials, such as hydrogels, that are used to mimic the extracellular matrix in tissue engineering. RAR uses a focused ultrasound pulse to generate a microscale perturbation at the sample surface and tracks the ensuing surface wave using pulse-echo ultrasound. The frequency spectrum of the resonant surface waves is analyzed to extract viscoelastic material properties. In this study, RAR was used to characterize fibrin, gelatin, and agarose hydrogels. Single time point measurements of gelled samples with static mechanical properties showed that RAR provided consistent quantitative data and measured intrinsic material characteristics independent of ultrasound parameters. RAR was also used to longitudinally track dynamic changes in viscoelastic properties over the course of fibrin gelation, revealing distinct phase and material property transitions. Application of RAR was verified using finite element modeling and the results were validated against rotational shear rheometry. Importantly, RAR circumvents some limitations of conventional rheology methods and can be performed in a high-throughput manner using conventional labware. Overall, these studies demonstrate that RAR can be a valuable tool to noninvasively quantify the viscoelastic mechanical properties of soft hydrogel biomaterials.

Survivability of Lactobacillus acidophilus and quality attributes of puffed pounded-unripe rice supplemented with probiotics using a fluidized-bed coating method

Value-added breakfast cereals are receiving increased interest due to changes in lifestyles and modern trends of health awareness among consumers. Among various cereal grains, puffed pounded-unripe rice (PPUR) was chosen because of its excellent health beneficial properties. Feasibility of supplementing the probiotic bacteria Lactobacillus acidophilus (LA5) into PPUR was investigated using a fluidized-bed coating method. Suitable fermentation conditions were tested based on a pH criterion of 4.5. Three types of cell host solutions as sucrose, skim milk and maltodextrin were examined as suitable carriers, and optimal coating condition was determined. Product qualities were evaluated for stability. Results showed that milk fermented for 24 hrs gave the highest viable cell count of approximately 109 to 1010 CFU/mL when optimally mixed with distilled water at milk: water ratio of 45:105. Skim milk was determined as the most suitable host giving the highest retention of LA5 cell count. Coating condition at a feed rate of 10 mL/min, coating time of 5 mins and constant temperature at 50°C gave the highest survival rates of LA5. Moisture contents and water activity (aw) values of all coated products ranged from 3.38-3.72% wb and 0.201-0.232, respectively. Regarding textural properties, coated PPUR showed increased hardness and crispiness compared with uncoated samples, while the variation of coating conditions had no significant effects. Dynamic changes in viable LA5 cell count, textural properties, aw, peroxide value and thiobarbituric acid values fitted well with various kinetic orders. Findings indicated the feasibility of adding probiotics to dry products associated with suitable coating solution and offered guidance for product development and process design.

Residual Mode Vector‐Based Structural Damage Identification with First‐Order Modal Information

Damage identification based on the change of dynamic properties is an issue worthy of attention in structure safety assessment, nevertheless, only a small number of discontinuous members in existing structure are damaged under service condition, and the most remaining members are in good condition. According to this feather, we developed an effective damage location and situation assessment algorithm based on residual mode vector with the first mode information of targeted structure, which utilized the quantitative relationship between first natural modes of global structure with the change of the element stiffness. Firstly, the element damage location is determined with exploitation of the sparseness of element stiffness matrices based on the discontinuity of damaged members. Then, according to the distribution characteristics of the corresponding residual mode vector, the nodal equilibrium equation about the damage parameter is established based on the residual mode vector, and the damage coefficients of structural elements are evaluated with the proposed equations. Two numerical examples are given to verify the proposed algorithm. The results showed that the proposed damage identification method is consistent with the preset damage. It can even accurately identify large‐degree damages. The proposed algorithm only required the first‐order modal information of the target structures and held few requirements of analysis resource; hence when compared with existing methods, it has obvious advantages for structural damage identification.

Changes of dynamic properties of mobile scenes at various steps of their manufacturing

Mobile stage construction is a huge challenge for designers due to a very wide range of variable factors influencing it, such as wind or temperature, the value of which in this case depends on the geographical location. An inherent element of the impact of these factors are the accompanying different, both constant and variable loads. Detailed identification of these loads is as important as the analysis of the dynamic properties of the structure. The more so as the analyzed structure appears in two configurations, first as a semitrailer, and then as a stage.

Response surface-based model updating to detect damage on reduced-scale masonry arch bridge

Response surface (RS) methods, a combination of mathematical and statistical techniques, have been widely used in design optimization, response prediction, and model validation in structural engineering systems. However, its usage in structural damage identification, especially for historic structures has not been quite common. For this purpose, this study attempts to investigate damage detection in a masonry arch bridge. Within the scope of this, a reduced-scale model of a one span historical masonry arch bridge was built in a laboratory environment. To determine the modal parameters of the reduced-scaled bridge model, operational modal analysis (OMA) was performed under ambient vibrations. Signals originated by sensitive accelerometers were collected to quantify the vibratory response of the reduced-scaled model bridge. The experimental natural frequencies, mode shapes, and damping ratios resulting from these measurements were figured out by using the Enhanced Frequency Domain Decomposition (EFDD) technique. The three-dimensional model of the reduced-scale bridge was created in the ANSYS finite element (FE) software program to expose the analytical dynamic characteristics of the bridge model. The results obtained in the experimental application were compared with those of the finite-element analysis of the bridge model. The calibration of the numeric model was utilized depending on the experimental modal analysis results of the reduced-scale bridge by using the RS method. Design of experiments was constructed by using central composite design, and the RS models were generated by performing the genetic aggregation approach. The optimum results between the experimental and numerical analyses were found by using the RS optimization. Then, regional damages created on the scaled model and the changes of dynamic properties of the damaged case were evaluated. The damage location was approximately identified by using the RS method in the calibrated finite-element model. The results demonstrated that the RS-based FE updating approach is an effective way for damage detection and localization in masonry type structures.

Shifts in the structure and function of wheat root-associated bacterial communities in response to long-term nitrogen addition in an agricultural ecosystem

Nitrogen (N) input in terrestrial ecosystems is increasing due to its deposition from anthropogenic activities, especially in agriculture, which can have pronounced effects on belowground microbial communities. However, our understanding of the long-term effects of N addition on microbial communities in the plant-soil system is still limited. Here, we used an Ion S5™ XL sequencing platform to explore the structural and functional characteristics of root-associated bacterial communities in winter wheat (Triticum aestivium) under long-term N addition. The N fertilization experiment involved five treatments (0, 90, 180, 270, and 360 kg N ha−1) in a farmland ecosystem over 14 years. Bacterial communities shifted significantly with increasing N level in three soil-root compartments (bulk soil, rhizosphere, and root interior) of wheat plants at two growth stages (jointing and filling). These shifts were associated with dynamic changes in soil properties, mainly pH, nitrate-N, and ammonium-N levels. There was a community shift between slow-growing oligotrophs and fast-grow copiotrophs with increasing N addition, indicating distinct survival strategies of bacteria across soil-root compartments and wheat growth stages. Potential biomarkers, including nitrifying, denitrifying, and N-fixing genera were identified to predict very high N concentration in each soil-root compartment. Significant variation was detected in the relative abundance of predicted functional genes related to N metabolism (denitrification, nitrification, and N fixation) across the three compartments, two growth stages, and five N levels. These results indicate that bacterial communities respond differentially to long-term high N input in the soil-root compartments of wheat plants during different growth stages.

Comparison of the Quantra QPlus System With Thromboelastography in Cardiac Surgery

Use of viscoelastic testing, such as thromboelastography (TEG), is recommended in cardiac surgery to monitor coagulation and to guide the transfusion of blood products. The Quantra QPlus System is a novel point-of-care platform that uses ultrasonic pulses to characterize dynamic changes in viscoelastic properties of a blood sample during coagulation. Despite the ability to assess similar aspects of clot formation, limited studies addressing the interchangeability of viscoelastic testing parameters exist. The primary aim of the present study was to assess the correlation and agreement between Quantra and TEG5000 results using blood samples from cardiac surgery patients. Tertiary care, academic medical center. Prospective observational study. Twenty-eight patients undergoing elective cardiac surgery undergoing cardiopulmonary bypass were evaluated. Perioperative blood samples were collected and assessed using Quantra, and results were compared with TEG and conventional coagulation testing. Method comparison analysis demonstrated that Quantra parameters (Quantra clot time, clot stiffness, and fibrinogen contribution to clot stiffness) significantly correlated with TEG R and TEG G after induction of anesthesia, during cardiopulmonary bypass, and after rewarming (rs = 0.83, rs = 0.84, and rs = 0.73, respectively). However, Quantra parameters demonstrated poor agreement compared with equivalent TEG5000 parameters. The Quantra QPlus System significantly correlated with TEG5000, suggesting that this test may be used in a similar clinical context. Despite the strength of correlation between Quantra and TEG parameters, measurements are not interchangeable.

Correlating Oxidation State and Surface Area to Activity from Operando Studies of Copper CO Electroreduction Catalysts in a Gas-Fed Device

The rational design of high-performance electrocatalysts requires a detailed understanding of dynamic changes in catalyst properties, including oxidation states, surface area, and morphology under realistic working conditions. Oxide-derived Cu catalysts exhibit a remarkable selectivity towards multi-carbon products for the electrochemical CO reduction reaction (CORR), but the exact role of oxide remains elusive for explaining the performance enhancements. Here, we used operando X-ray absorption spectroscopy (XAS) coupled with simultaneous measurements of catalyst activity and selectivity by gas chromatography (GC) to study the relationship between oxidation states of Cu-based catalysts and activity for ethylene (C2H4) production in a CO gas-fed cell. By utilizing a custom-built XAS cell, oxidation states of Cu catalysts can be probed in device-relevant settings and under high current densities (>80 mA/cm-2) for CORR. By employing an electrochemical oxidation process, we found that the Cu oxidation states and specific ion species do not correlate with C2H4 production. The difference in CORR activity is also investigated in relation to the electrochemical surface area (ECSA) changes. While hydrogen evolution reaction (HER) activity is positively correlated to the ECSA changes, the increased C2H4 activity is not proportional to ECSA. Ex-situ characterization from microscopic techniques suggests that the changes in C2H4 activity and selectivity may arise from a morphological transformation that evolves into a more active structure. These comprehensive results give rise to the development of a cell regeneration method that can restore the performance of Cu catalyst without cell disassembly. Our study establishes a basis for the rational design of highly active electrocatalysts for broad-range reactions in a gas-fed device.

In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota

The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota. Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions. Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota. Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota. LLP-I could also promote the production of health-promoting short chain fatty acids. Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.

Synthesis and properties of styrenic triblock copolymers with dual structural asymmetry via RAFT emulsion polymerization

The polystyrene-block-poly(n-butyl acrylate)-block-polystyrene (PSt-b-PBA-b-PSt, SBAS) triblock copolymers (TBCPs) with dual structural asymmetry were synthesized via a reversible addition-fragmentation chain transfer (RAFT) emulsion polymerization. The dual structural asymmetry contained two asymmetric outer PSt blocks and a single short ionic block on one chain end while the SBAS TBCPs had the almost same molecular weight (~100 kDa) and composition (~70 wt% of PSt and ~30 wt% of PBA) excluding the short ionic block. The dual structural asymmetry with a synergistic effect was designed by locating the long PSt block near the short ionic block that was derived from ionic macromolecular RAFT agent. With the SBAS TBCPs having microphase separation structure, the structural asymmetry of two outer PSt blocks tailored the interaction of PSt and PBA blocks, further leading to the change of static tensile and dynamic mechanical properties, and rheology. Structural asymmetry will provide an alternative to tailor-made properties of TBCPs.

Bimeron clusters in chiral antiferromagnets

A magnetic bimeron is an in-plane topological counterpart of a magnetic skyrmion. Despite the topological equivalence, their statics and dynamics could be distinct, making them attractive from the perspectives of both physics and spintronic applications. In this work, we demonstrate the stabilization of bimeron solitons and clusters in the antiferromagnetic (AFM) thin film with interfacial Dzyaloshinskii–Moriya interaction (DMI). Bimerons demonstrate high current-driven mobility as generic AFM solitons, while featuring anisotropic and relativistic dynamics excited by currents with in-plane and out-of-plane polarizations, respectively. Moreover, these spin textures can absorb other bimeron solitons or clusters along the translational direction to acquire a wide range of Néel topological numbers. The clustering involves the rearrangement of topological structures, and gives rise to remarkable changes in static and dynamical properties. The merits of AFM bimeron clusters reveal a potential path to unify multibit data creation, transmission, storage, and even topology-based computation within the same material system, and may stimulate spintronic devices enabling innovative paradigms of data manipulations.

Management of the dynamic properties of a base modified by deep soil mixing technology

Conversion of building properties is performed primarily to reduce the compressibility of the base and increase strength. However, in addition to changing the static properties of soils, it is necessary to take into account changes in dynamic properties, such as dynamic shear modulus, damping coefficient, velocity of elastic shear and longitudinal waves. Thus, it is possible to control the dynamic properties of the base by converting the construction properties of soils. In this paper, we consider the change in the velocity of shear waves in a base transformed by the technology of deep soil mixing (DSM). In the work, the influence of additional vertical stresses on the dynamic properties of the bases transformed using deep soil mixing technology was evaluated. As a parameter that allowed us to evaluate the dynamic properties, the propagation velocity of elastic shear waves was chosen. Shear wave velocity was estimated based on the results of triaxial tests using the method of low-amplitude torsional vibrations in a resonant column. The research results showed that at small values of axial strains, there is no significant increase in the velocity of shear waves, an increase in the speed of shear waves by two times with an increase in stress by 0.6 MPa.

Microstructure, mechanical properties, and corrosion resistance of friction stir welded Mg-Al-Zn alloy thick plate joints

Mg-Al-Zn (AZ31) of 10-mm thick plates were subjected to friction stir welding (FSW) using a conventional tool (CT) and bobbin tool (BT), respectively. The microstructure, room temperature tensile properties, dynamic compressive mechanical properties, and corrosion resistance of the base metal (BM) and various regions of the friction stir welded joints were investigated systematically. The mean grain size, dislocation density, and precipitate distribution in the various regions of the friction stir welded joints were various due to different thermal cycle and plastic deformation. Compared with the CT, a relatively more homogeneous microstructure, a larger number of high-density dislocations and precipitates were produced in the various regions of the joint prepared by the BT due to higher thermal cycle and strain rate. As a result, the friction stir welded joint prepared by the BT exhibits excellent tensile properties and dynamic compressive mechanical properties, and outstanding corrosion resistances compared to those of the CT. In addition to slight changes in tensile properties and dynamic compressive mechanical properties, the corrosion resistance of the joint prepared by the BT was significantly improved compared to the BM. FSW with BT was suggested to be the optimal method to weld AZ31 alloy thick plate.

Electronic, bonding and elastic properties of the ordered $${\hbox {SrTi}}_{1-x} {\hbox {Zr}}_{x} \hbox {O}_{3}$$ alloys: a first principles study

By means of first-principles calculations based on the density functional theory (DFT), we have investigated the structural, elastic, electronic, and bonding properties of three $$\hbox {SrTi}_{1-x} \hbox {Zr}_{x} \hbox {O}_{3}$$ alloys. The study shows that the substitution of Ti ion by the Zr one can also be undertaken by a tetragonal structure. However, due to the similar ionic radii between substitute cation, the choice of this super-cell leads to a weak change in both structural and dynamical properties and behave similarly even under different applied strains. Electronic as well as bonding properties are more affected by the substitution due to the rearrangement of the atomic orbitals. The calculations of band gaps depict a possible use of the investigated alloys in many UV device applications. Additionally, we will show that deep insight of bonding properties depicts some difference in ionicity degree between alloys. This trend is due essentially to the change in electron valence resulted in a weak variation of energetic orbitals.

Higher economic benefits and changes in soil fertility due to intensifying winter crop rotation in double-rice cropping systems

Farmers are increasingly planting vegetables during the winter fallow period in the double-rice growing area of Guangdong Province, southern China. The intensified winter crop rotation system (rice-rice-vegetable, RRV) may improve the annual planting benefit and change the soil fertility. To address this, yields, biomass, and profitability of each crop in the RRV, relative to the traditional rice-rice-fallow system (RRF), were compared, and the dynamics of physical, chemical and biological properties of soil at different crop stages were determined in this paper. The annual crop yield, biomass and economic return of RRV were significantly higher than those of RRF due to the inclusion of winter vegetables (green beans) in the cropping system. The contribution of green beans to the total revenue per year for RRV was as high as 73%. Filling winter fallow with green beans had no influence on the yield of early and late rice. Soil NO3−-N, available P, exchangeable K, exchangeable Fe, exchangeable Cu and acid phosphatase activity at the winter stage were significantly higher in RRV than in RRF, whereas soil pH, soil organic carbon (SOC), dissolved organic carbon (DOC), exchangeable Ca, exchangeable Mg, catalase activity, dehydrogenase activity and invertase activity at the winter stage were lower. The soil NO3−-N, exchangeable Fe, exchangeable Cu, and acid phosphatase activity during the early rice stage also tended to be higher in the RRV system. The annual dynamics of soil physical, chemical and biological properties were proven to be greater in the RRV system than in the RRF system. The findings of our study contribute to the understanding of dynamic changes in physical, chemical and biological properties of soil across different crop stages due to intensified green bean planting during the winter fallow period in a double-rice cropping system, which will be useful for the sustainable development of RRV.

Deforestation and reforestation impacts on soils in the tropics

Soils under natural, tropical forests provide essential ecosystem services that have been shaped by long-term soil–vegetation feedbacks. However, deforestation of tropical forest, with a net rate of 5.5 million hectares annually in 2010–2015, profoundly impacts soil properties and functions. Reforestation is also prominent in the tropics, again altering the state and functioning of the underlying soils. In this Review, we discuss the substantial changes in dynamic soil properties following deforestation and during reforestation. Changes associated with deforestation continue for decades after forest clearing eventually extend to deep subsoils and strongly affect soil functions, including nutrient storage and recycling, carbon storage and greenhouse gas emissions, erosion resistance and water storage, drainage and filtration. Reforestation reverses many of the effects of deforestation, mainly in the topsoil, but such restoration can take decades and the resulting soil properties still deviate from those under natural forests. Improved management of soil organic matter in converted land uses can moderate or reduce the ecologically deleterious effects of deforestation on soils. We emphasize the importance of soil knowledge not only in cross-disciplinary research on deforestation and reforestation but also in developing effective incentives and policies to reduce deforestation. Deforestation and reforestation cause dramatic changes to tropical ecosystems, including underlying soil properties and their corresponding ecosystem services. In this Review, the impacts of this land-use change on soils and their functions are discussed.

Dynamic dielectric properties characterization of tapered dielectrophoresis microelectrodes for selective detection and rapid manipulation of cells

PurposeThis paper aims to present the dielectrophoresis (DEP) force (FDEP), defined as microelectrofluidics mechanism capabilities in performing selective detection and rapid manipulation of blood components such as red blood cells (RBC) and platelets. The purpose of this investigation is to understand FDEP correlation to the variation of dynamic dielectric properties of cells under an applied voltage bias.Design/methodology/approachIn this paper, tapered design DEP microelectrodes are used and explained. To perform the characterization and optimization by analysing the DEP polarization factor, the change in dynamic dielectric properties of blood components are observed according to the crossover frequency (fxo) and adjustment frequency (fadj) variation for selective detection and rapid manipulation.FindingsExperimental observation of dynamic dielectric properties change shows clear correlation to DEP polarization factor when performing selective detection and rapid manipulation. These tapered DEP microelectrodes demonstrate an in situ DEP patterning efficiency more than 95%.Research limitations/implicationsThe capabilities of tapered DEP microelectrode devices are introduced in this paper. However, they are not yet mature in medical research studies for various purposes such as identifying cells and bio-molecules for detection, isolation and manipulation application. This is because of biological property variations that require further DEP characterization and optimization.Practical implicationsThe introduction of microelectrofluidics using DEP microelectrodes operate by selective detecting and rapid manipulating via lateral and vertical forces. This can be implemented on precision health-care development for lab-on-a-chip application in microfluidic diagnostic and prognostic devices.Originality/valueThis study introduces a new concept to understand the dynamic dielectric properties change. This is useful for rapid, label free and precise methods to conduct selective detection and rapid manipulation of mixtures of RBC and platelets. Further, potential applications that can be considered are for protein, toxin, cancer cell and bacteria detections and manipulation. Implementation of tapered DEP microelectrodes can be used based on the understanding of dynamic dielectric properties of polarization factor analysis.

Wind-resistant optimal design of tall buildings based on improved genetic algorithm

Modern tall buildings are likely sensitive to wind excitations due to their increasing height and structural flexibility. Proper or optimal wind-resistant structural design of such high-rise buildings is required or sometimes necessary to meet the requirement of safety and serviceability. Combining a genetic algorithm (GA) with improved penalty function and calculation method for equivalent static wind loads (ESWLs), this paper presents an optimal procedure for the wind-resistant design of tall buildings, which aims to minimize the total weight of the designed building subject to the constraints of top acceleration and lateral drifts. A penalty function is utilized to deal with the implicit expressed constraints of optimization. The ESWLs can be instantaneously updated due to the change of structural dynamic properties during the optimal design process. A 60-story rectangular reinforced concrete frame structure with the same geometric shape of the CAARC standard tall building is adopted to verify the feasibility of the presented method. The example demonstrates that the proposed optimal design procedure is an effective tool for the wind-resistant optimal design of tall buildings.

Dynamic evolution of electrochemical and biological features in microbial fuel cells upon chronic exposure to increasing oxytetracycline dosage

Dynamic changes in power generation and electrochemical properties were compared between the control microbial fuel cells (C-MFC) and an oxytetracycline (OTC)-treated MFC (O-MFC) on days 84, 139, 174, 224, 295, 307 and 353. The results showed that a high concentration of OTC (>5 mg·L−1) could inhibit microbial activity and result in a decline of voltage output and power density compared with the same C-MFC. However, with the prolongation of incubation time, the inhibitory effect was gradually weakened. Electrochemical analyses demonstrated that long-term OTC acclimation reduced the ohmic and polarisation resistance of the anode, which was conducive to the recovery of electrochemical performance. More than 99% of 10 mg·L−1 OTC could be removed within 48 h, and the antibacterial activity of the MFC effluent on Escherichia coli DH5α was conclusively eliminated. High-throughput sequencing analysis revealed that the diversity and richness of the microbial community decreased significantly after long-term OTC enrichment. Acinetobacter, Petrimonas, Spirochaetaceae and Delftia were enriched and played a dominant role in C-MFC stability and power generation. The promotion by Cupriavidus, Geobacter and Stenotrophomonas in simultaneous OTC degradation and bioelectricity generation in the O-MFC was demonstrated.

Comparative study of instrumental properties and sensory profiling of low-calorie chocolate containing hydrophobically modified inulin. Part II: Proton mobility, topological, tribological and dynamic sensory properties

The aim of the present work was to evaluate the changes in instrumental measurements and dynamic sensory properties of chocolate as affected by the partial and total substitution of sucrose by dodecenyl-succinylated inulin upon storage. The results of textural, surface topology, and tribological properties of non-stored chocolates were found to be a function of modified inulin replacement, where an increase in modified inulin ratio allowed an increase in hardness, roughness, and friction coefficient. Compared to non-stored samples, the deterioration of textural properties after storage was inhibited in chocolate containing higher levels of modified inulin. Higher modified inulin ratios did not also induce an important change in NMR proton mobility, roughness, and friction coefficient upon storage. Confocal Raman microscopy revealed modified inulin located around sucrose and cocoa particles dispersed in fat phase, especially at the higher ratios. Temporal Dominance of Sensations (TDS) curves of non-stored samples elucidated that firmness was a dominant perception at the beginning of mastication, followed by melting rate, while cocoa flavor was experienced at the end of evaluation. However, these attributes were not perceived in stored chocolate with the lowest modified inulin. A PCA was performed to compare TDS scores and instrumental readings, showing a high correlation between instrumental measurement of hardness, friction coefficient, and NMR relaxation times and TDS score of firmness, bitterness, astringency, and melting rate. This work shows a new perspective to use biopolymeric surfactant for producing low-calorie chocolate, considering that hydrophobically modified inulin can diminish production costs and facilitate low-calorie product development.