Changes In Spectral Composition Research Articles

Daylighting Through Electrochromic Glazing Under Overcast Skies: Impacts on Visual Task Performance and Perception

ABSTRACT Electrochromic (EC) glazing allows modulating the intensity of visual and solar transmission by dynamically switching between bleached (clear) and tinted (colored) states, leading to changes in spectral power distribution (SPD), correlated color temperature (CCT), and illuminance of the incoming daylight. In this experimental study, we investigated the impact of spectral composition of daylight filtered through a 6-pane EC façade on visual task performance and visual perception. In a semi-controlled test room, 19 subjects were exposed to five setting scenarios of the EC façade (fully bleached, mixed setting after fully bleached, fully tinted, mixed setting after fully tinted, and user-controlled). Test subjects performed a series of visual tasks, and their assessments of the indoor environment and of the outside view were collected along with luminous and spectral measurements under each condition. The analysis provided statistical evidence that the changes in spectral composition of daylight do not have a practically relevant effect on visual task performance in terms of visual acuity and color naming accuracy. However, the daylight filtered through the fully tinted glazing had a substantive impact on visual perception, evoking negative responses to the color rendering of the indoor environment and of the outside view. A mixed settings of the EC façade could improve a natural assessment of the incoming light compared to the fully tinted state, achieving better ratings in terms of perception of the indoor and outdoor environments. When given control of the EC glazing, subjects expressed higher acceptance and satisfaction compared to the tinted and mixed scenarios.

Changes in the light environment: Short-term responses of photosynthesis and metabolism in spinach.

Modification in the light environment can induce several changes even within a short time. In this article, light intensity and spectrum-dependent changes in photosynthetic and metabolic processes were investigated in spinach leaves. Short-term exposure of the youngest fully developed leaves provided an elevated CO2 assimilation capacity under red light compared with blue or white light, although the electron transport rate was lower. The stomatal opening was mainly stimulated by blue light. These spectrum-induced changes also depended on light intensity. When white light was used to activate the photosynthesis, the white light showed a similar light response to blue light regarding the electron transport processes and red light in terms of stomatal opening. In contrast, concerning CO2 assimilation characteristics, the white light resembled blue light at low and red light at high light intensities. These results indicate that the photosynthetic processes strongly interact with the light intensity and spectral composition. Furthermore, changes in spectral composition modified the primary metabolic processes as well. Red light induced the sugar accumulation, while more organic acids that belong to the respiration pathway were produced under blue and white lights. These changes occurred even within a short (30 min) time frame. These results also draw attention to the importance of the light environment used during the measurements of the photosynthetic activity of plants and/or sample collections.

Fields of a thousand shimmers: canopy architecture determines high-frequency light fluctuations.

Wind-induced movement in the canopy produces rapid fluctuations in irradiance, called 'windflecks'. They create a dynamic environment for photosynthesis that bears little resemblance to the stable controlled conditions under which plants are typically measured. We recorded time series of irradiance to assess the diversity of windfleck properties (intensity, duration, frequency, clustering, and spectral composition) in canopies of four crops and five tree species. We also measured traits associated with leaf morphology and canopy architecture, which could be associated with canopy-specific differences in windflecks. Distinct features of windfleck properties were identified both between and among crop and tree canopy. Windflecks in crops were generally more intense and longer, and baseline irradiance was much higher than even the peak irradiance during a windfleck in a forest. The change in spectral composition during a windfleck was species-specific. Overall, irradiance fluctuations were less frequent and less intense in tall canopies and with increased depth from the canopy. Our systematic exploration of how canopy structure dictates light dynamics provides new insight into windfleck creation. Coupled with progress in elucidation of the mechanisms of photosynthetic induction, this knowledge should improve our capacity to model canopy ecophysiology and understand light use efficiency in shade.

Rapid changes in spectral composition after darkness influences nitric oxide, glucose and hydrogen peroxide production in the Antarctic diatom Fragilariopsis cylindrus

Ice-associated phototrophic taxa contribute significantly to Antarctic primary production and are crucial to ecosystem stability in the Southern Ocean. The quantity and quality of light required for photosynthesis is the single most influential driver of ice-associated algal communities. While the presence of ice and snow greatly reduces the irradiance reaching the ice-water interface, it is also the spectral quality that influences the phototrophy of ice-associated microalgae communities. Here we test the capability of three electrochemical microsensors to detect photosynthetically derived “stress” metabolites produced by the Antarctic diatom F. cylindrus. Following a period of dark incubation, this photo-physiological response differed with respect to the intensity and spectral quality of light during re-illumination. Exposure to blue light resulted in impairment in photosynthetic efficiency of PSII (Fv/Fm) and resulted in the production of nitric oxide (NO), hydrogen peroxide (H2O2) and glucose exudation. A similar trend in metabolite production was observed when subjected to white light, but not during red or green illumination. These results indicate that rapid exposure to light and variation in spectral composition can cause significant stress that can be quantified using H2O2, NO and glucose microsensors. This metabolic overflow was triggered by the disruption of normal photosynthetic electron flow and it is proposed that the detection of extracellular metabolites can be directly attributed to intracellular activity.

Effects of Light Intensity, Spectral Composition, and Paclobutrazol on the Morphology, Physiology, and Growth of Petunia, Geranium, Pansy, and Dianthus Ornamental Transplants

Under light-limiting conditions, many ornamental greenhouse-grown plants show undesired morphological characteristics, such as plant elongation (hypocotyl and epicotyl length) and low dry mass, which reduce plant quality. Research has shown that use of plant growth regulators (PGRs) and changes in both light intensity and spectral composition can reduce these undesired characteristics. However, little is known about the role of the combined effects of supplemental lighting and PGRs on the production of ornamental seedlings. The objective of this study was to characterize the combined and independent effects of light intensity, spectral composition, and PGR applications on the greenhouse production of ornamental transplants. Petunia (Petunia × hybrida), geranium (Pelargonium × hortorum), pansy (Viola × wittrockiana) and dianthus (Dianthus chinensis) were grown for 32–42 days under three supplemental light (SL) treatments: 1) high-pressure sodium (HPS), 2) light-emitting diodes (LEDs) with a 6 blue (B):5 green (G):89 red (R) (percent photon flux ratio), and 3) LEDs with 19B:81R (100 μmol m−2 s−1, 18 h photoperiod for all treatments). A control (No SL) was also included. In addition, a portion of plants were also sprayed with the paclobutrazol PGR (PBZ and No PBZ). The synergistic effects of the combination of PBZ and supplemental lighting resulted in the most compact plants, caused by a reduction in plant height by PBZ and an increase in dry mass by SL. However, PBZ reduced shoot dry mass of most plant species and light combinations. Plant compactness was greater under the 6B:5G:89R LED composition for petunia and when combined with PBZ for geranium than for plants under HPS lighting. Root dry mass of petunia, geranium, and pansy plants increased in response to SL compared with no SL by 2.4–5.7-fold. Results from the two LED spectra were unexpected; plants under 6B:5G:89R were more compact (petunia, geranium), had higher anthocyanin concentrations (petunia), were shorter (petunia, pansy, dianthus) and had less leaf area (petunia, pansy, dianthus) than plants in the SL treatment with a higher B and lower G PF (19B:81R). Supplemental lighting and PBZ can be used in conjunction or independently to improve plant morphology. The increased light from SL provided the most benefits by improving dry mass, compactness, and leaf number for most plant species. However, when PBZ was used in combination with SL, plant compactness increased for some species. The spectral composition of SL had an impact on plant growth and morphology, warranting additional research on plant responses to small changes in the spectral composition of SL.

Cones Support Alignment to an Inconsistent World by Suppressing Mouse Circadian Responses to the Blue Colors Associated with Twilight.

SummaryIn humans, short-wavelength light evokes larger circadian responses than longer wavelengths [1, 2, 3]. This reflects the fact that melanopsin, a key contributor to circadian assessments of light intensity, most efficiently captures photons around 480 nm [4, 5, 6, 7, 8] and gives rise to the popular view that “blue” light exerts the strongest effects on the clock. However, in the natural world, there is often no direct correlation between perceived color (as reported by the cone-based visual system) and melanopsin excitation. Accordingly, although the mammalian clock does receive cone-based chromatic signals [9], the influence of color on circadian responses to light remains unclear. Here, we define the nature and functional significance of chromatic influences on the mouse circadian system. Using polychromatic lighting and mice with altered cone spectral sensitivity (Opn1mwR), we generate conditions that differ in color (i.e., ratio of L- to S-cone opsin activation) while providing identical melanopsin and rod activation. When biased toward S-opsin activation (appearing “blue”), these stimuli reliably produce weaker circadian behavioral responses than those favoring L-opsin (“yellow”). This influence of color (which is absent in animals lacking cone phototransduction; Cnga3−/−) aligns with natural changes in spectral composition over twilight, where decreasing solar angle is accompanied by a strong blue shift [9, 10, 11]. Accordingly, we find that naturalistic color changes support circadian alignment when environmental conditions render diurnal variations in light intensity weak/ambiguous sources of timing information. Our data thus establish how color contributes to circadian entrainment in mammals and provide important new insight to inform the design of lighting environments that benefit health.

Pigment variations in Emiliania huxleyi (CCMP370) as a response to changes in light intensity or quality.

Many studies on photoacclimation examine the pigment responses to changes in light intensity, but variations in light climate in the aquatic environment are also related to changes in spectral composition. We have employed a high-performance liquid chromatography method with improved resolution towards chlorophyll c and fucoxanthin-related xanthophylls to examine the pigment composition of Emiliania huxleyi CCMP 370 under different light intensities and spectral qualities. To maintain its photosynthetic performance, E. huxleyi CCMP370 promotes drastic pigment changes that can be either the interconversion of pigments in pools with the same basic chromophoric structure (Fucoxanthin type or chlorophyll c type), or the ex novo synthesis (Diatoxanthin). These changes are linked either to variations in light quality (Fucoxanthin related xanthophylls) or in light intensity (chlorophyll c3 /Monovinyl chlorophyll c3 , Diadinoxanthin/Diatoxanthin, β,ɛ-carotene/ β,β-carotene). Fucoxanthin and 19'-hexanoyloxyfucoxanthin proportions were highly dependent on spectral conditions. Whereas Fucoxanthin dominated in green and red light, 19'-hexanoyloxyfucoxanthin prevailed under blue spectral conditions. Our results suggest that the huge pigment diversity enhanced the photoacclimative capacities of E. huxleyi to efficiently perform under changing light environments. The ubiquity and success in the global ocean as well as the capacity of E. huxleyi to form large surface blooms might be associated to the plasticity described here.

Colour as a signal for entraining the mammalian circadian clock.

Twilight is characterised by changes in both quantity (“irradiance”) and quality (“colour”) of light. Animals use the variation in irradiance to adjust their internal circadian clocks, aligning their behaviour and physiology with the solar cycle. However, it is currently unknown whether changes in colour also contribute to this entrainment process. Using environmental measurements, we show here that mammalian blue–yellow colour discrimination provides a more reliable method of tracking twilight progression than simply measuring irradiance. We next use electrophysiological recordings to demonstrate that neurons in the mouse suprachiasmatic circadian clock display the cone-dependent spectral opponency required to make use of this information. Thus, our data show that some clock neurons are highly sensitive to changes in spectral composition occurring over twilight and that this input dictates their response to changes in irradiance. Finally, using mice housed under photoperiods with simulated dawn/dusk transitions, we confirm that spectral changes occurring during twilight are required for appropriate circadian alignment under natural conditions. Together, these data reveal a new sensory mechanism for telling time of day that would be available to any mammalian species capable of chromatic vision.

Year-round variability of ambient noise in temperate freshwater habitats and its implications for fishes

Changes in habitat acoustics over the year can potentially affect fish hearing and orientation to sound, especially in temperate climates. This is the first study where year-round changes in ambient noise in aquatic habitats were assessed. Seven different European fresh-water habitats were chosen for this study. Sound pressure level (SPL) and spectral composition of the ambient noise varied in both quiet stagnant habitats (lakes, backwaters) and in flowing habitats (streams, rivers). Linear equivalent SPL (L(Leq, 60s)) tended to be lower in stagnant habitats (means: 91.6-111.7 dB) than in flowing habitats (means: 111.2-133.4 dB). The changes in SPL were smallest in the river (means: 4.2-4.4 dB, maxima: 8.5-10.1 dB), whereas significantly higher values were measured in stagnant habitats and the stream (means: 9.9-14.9 dB, maxima: 25.1-30.9 dB). The spectral compositions of the ambient noise determined at different times of the year were highly correlated to each other at the river sites (mean cross-correlation coefficients: 0.85 and 0.94) and were weaker or not correlated at the other study sites (means: 0.24-0.76). The changes in ambient noise spectra were negatively correlated to changes in SPL, indicating that large changes in SPLs were accompanied by large changes in spectral composition and vice versa. Comparison of these ecoacoustical data with a preceding study (Amoser and Ladich in J Exp Biol 208:3533-3542, 2005) indicates that the auditory sensitivity in hearing specialists is affected by changes in ambient noise levels and spectra throughout a year and that this effect tends to be more pronounced in stagnant waters and the stream than at river sites. On the other hand, absolute noise levels result in a higher degree of masking in flowing waters.

Effect of BiI3 doping on the optical properties of CaI2

The effect of BiI3 doping on the optical absorption spectra and roentgeno-, photo-, and thermoluminescence of CaI2 scintillator crystals has been studied in the temperature range 90–295 K. The crystals were grown by the Bridgman-Stockbarger method. Doping of CaI2 with BiI3 from the melt gives rise to absorption bands centered at 466, 400, and 350 nm, which can be interpreted as the A, B, and C bands due to electronic transitions from the 1S0 state to the 3P1, 3P2, and 1P1 levels of a free Bi3+ ion. The absorption band at 270–290 nm is assignable to near-activator excitons. Changes in spectral composition and the reduction in luminescence intensity caused by Bi3+ doping of CaI2 are associated mainly with the reabsorption of the emission from centers characteristic of the host by activator centers. Under x-ray excitation, the spectrum of heavily doped crystals shows, in addition, a weak emission centered around 620 nm, which is probably due to an impurity phase. The light sum of CaI2:Bi3+ under x-ray excitation is small and is due to shallow traps. Upon Bi3+ substitution on the cation site of CaI2, the excess charge of the activator is probably compensated by unintentional O2− impurity and vacancy pairs near Bi3+ centers—one vacancy in a neighboring cation site and the other in a neighboring anion site.

The role of space charge formation in the generation of thermally stimulated current (TSC) spectroscopy data for a model amorphous drug system

Thermally stimulated current (TSC) spectroscopy is attracting increasing attention as a means of materials characterization, particularly in terms of measuring slow relaxation processes in solid samples. However, wider use of the technique within the pharmaceutical field has been inhibited by difficulties associated with the interpretation of TSC data, particularly in terms of deconvoluting dipolar relaxation processes from charge distribution phenomena. Here, we present evidence that space charge and electrode contact effects may play a significant role in the generation of peaks that have thus far proved difficult to interpret. We also introduce the use of a stabilization temperature in order to control the space charge magnitude. We have studied amorphous indometacin as a model drug compound and have varied the measurement parameters (stabilization and polarization temperatures), interpreting the changes in spectral composition in terms of charge redistribution processes. More specifically, we suggested that charge drift and diffusion processes, charge injection from the electrodes and high activation energy charge redistribution processes may all contribute to the appearance of shoulders and ‘spurious’ peaks. We present recommendations for eliminating or reducing these effects that may allow more confident interpretation of TSC data.

Tall Fescue Photomorphogenesis as Influenced by Changes in the Spectral Composition and Light Intensity

The influence of deciduous foliage shade on turfgrass development has not been fully investigated. Previous research neglects changes in spectral distribution, e.g., red:far‐red light (R:FR) ratios common of foliage shade. Turfgrass plants may respond simultaneously but in different ways to changes in light intensity and spectral composition. A field study was conducted in 2001–2002 at the Ohio Turfgrass Research and Educational Facility, Columbus, OH. Two tall fescue (Festuca arundinacea Schreb.) cultivars of differing shade tolerance were established under low photosynthetic photon flux (PPF) in approximately 8% of full sunlight with high (>1) and low (<1) R:FR ratios to distinguish between developmental effects of R:FR ratio (spectral composition) and PPF (light intensity) on turfgrass photomorphogenesis. Few morphological differences in shade tolerance between the two cultivars were observed during the 2‐yr study. However, under low PPF, high R:FR ratios led to increased tillering, leaf blade width and thickness, and chlorophyll contents. Root mass declined under reduced PPF regardless of R:FR ratio. Results suggest that while turfgrass photomorphogenesis in shade is influenced by changes in PPF, many characters are further influenced by changes in the R:FR ratio.

Proposed metameric indices for goniochromatic objects

As a means of determining the degree of metamerism for goniochromatic objects, a generalized illuminant metamerism index for changes in spectral composition of illuminants has been formulated based on a modified LABD index. This index takes into account factors such as color memory and could be used under paramerism conditions. Consequently, a geometric metamerism and a flop index is also presented. Nevertheless, their estimation is based on the establishment of goniochromatic discrimination functions. All these concepts could be useful in many industrial-coating applications to achieve an overall measure of variation in goniochromatism on metallic materials.

Coding of concurrent vocal signals by the auditory midbrain: effects of stimulus level and depth of modulation.

The segregation of concurrent vocal signals is an auditory processing task faced by all vocal species. To segregate concurrent signals, the auditory system must encode the spectral and temporal features of the fused waveforms such that at least one signal can be individually detected. In the plainfin midshipman fish (Porichthys notatus), the overlapping mate calls of neighboring males produce acoustic beats with amplitude and phase modulations at the difference frequencies (dF) between spectral components. Prior studies in midshipman have shown that midbrain neurons provide a combinatorial code of the temporal and spectral characteristics of beats via synchronization of spike bursts to dF and changes in spike rate and interspike intervals with changes in spectral composition. In the present study we examine the effects of changes in signal parameters of beats (overall intensity level and depth of modulation) on the spike train outputs of midbrain neurons. The observed changes in spike train parameters further support the hypothesis that midbrain neurons provide a combinatorial code of the spectral and temporal features of concurrent vocal signals.

光質および光強度がアサガオの花芽形成に及ぼす影響

Flowering of morning glory (Pharbitis nil Choisy) occurs during the summer season in Japan. This species is well-known as a typical short-day plant. It is, however, not clear why morning glory flowers in summer season under long-day conditions. To answer this question, we analyzed the seasonal changes in the spectral characteristics of solar radiation and found that the ratio of blue component to photosynthetically active radiation increased in summer and decreased in winter, whereas the reverse held for green, and that of red did not undergo much changes throughout the year. In the present study, we investigated the effect of light quality on flower-bud formation in morning glory (cv. Murasaki), using monochromatic lights. Flowerbud formation occurred much earlier under blue light than either green or red light. It was accelerated by increasing the irradiance of blue light and the ratio of blue light to green light. We suggest that the seasonal changes in spectral composition of solar radiation, particularly the rate of blue component, can be one of the factors in controlling the flower-bud formation of morning glory.

Spectral Composition of Light as a Zeitgeber for Birds Living in the High Arctic Summer

The hypothesis was tested whether periodic changes in spectral composition of light are an effective zeitgeber for synchronization (entrainment) of circadian rhythms of birds breeding in the high arctic summer. Two palearctic passerine birds, the brambling ( Fringilla montifringilla) and the common redpoll ( Carduelis f. flammea), which both breed during summer at latitudes above the Arctic Circle and migrate to temperate zone latitudes for wintering, were studied. To investigate the effect of daily alterations in light spectrum, bramblings were tested under 24-h cycles of fluorescent lights of different color temperatures (12 h at ca 5,000 K and 12 h at ca 2,500 K) and energy ratios varying between 16:1 and 0.3:1. Entrainment of activity rhythms occurred at energy ratios of 1:1 (in all seven birds tested) and 0.8:1 (in three of seven birds tested) with activity mostly within the “blue” phase (ca 5,000 K). Light cycles alternating between narrow spectral ranges of blue (max. at 440 nm) and red (max. at 650 nm) and energy ratios of 1:1 and 1.1:1 (equivalent to ratios of photons flux of ca 0.67:1 and 0.73:1, respectively) were also effective in entraining the bramblings' activity rhythms (8 and 9 of 11 birds, respectively) with activity occurring primarily during the red phase. The activity rhythms of most common redpolls tested also entrained to blue:red light cycles (B:R 16.5:7.5 h) with equal energies during the blue and red phase (equivalent to a photons flux ratio of ca 0.67:1). The results support the hypothesis that passerine birds breeding in the arctic during summer may use the daily changes in spectal composition of sunlight as a zeitgeber for synchronizing physiological and behavioral rhythms, if changes in light intensity are not a reliable environmental cue.

Spectroscopic analysis of proton-induced fluorescence from yttrium orthosilicate

The results of a 3-MeV-proton irradiation test on two yttrium orthosilicate doped with cerium (YOS:Ce) crystal samples are reported. The principle goal of this test was to determine the proton dose required to reduce the resulting YOS:Ce fluorescence light to half of its original value (half brightness dose) at ambient temperature and 150 degrees C. Results from this test will also provide basic information concerning potential changes in spectral composition and fluorescence peak widths for YOS:Ce at ambient temperature and 150 degrees C.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>

Influence of orchard management system on leaf angle and leaf orientation in apple trees

The effect of six orchard management systems on the display of leaves in the canopies of apple trees was compared. Canopies which are strongly formalized through the use of trellises can change the way leaves are orientated. Small differences in leaf angle, leaf orientation, and dispersion (variation in angles and orientations) were found between free standing and trellised trees. These differences were consistent for two cultivars, Granny Smith and Starking Delicious, although stronger for the latter. Leaves from freestanding open canopies have a wider variety of angles and orientation that those from trellised canopies. Changes in leaf orientation did not appear to relate to changes in spectral composition which was similar for all canopies. A tentative claim for evidence of phototropism by leaves in some parts of some canopies is also made.

Effects of illumination on the rabbit's EEG during the two phases of its circadian rhythm.

After exposure to a LD 12:12 regimen for several weeks, rabbits exhibit a programmed circadian rhythm in the RMS values of their occipital and frontal EEG's during 54-hr recordings in constant darkness. Illumination at levels of 80, 160 and 230 Lx raises these RMS values in both phases of the rhythm. The induced rise is large in the phase with low RMS values and small in the other, whereas light-induced changes in spectral composition of the EEG's are slight in either phase. Bilateral optic nerve sectioning results in similar changes in properties of the EEG and in the amplitude of its rhythm. The results are discussed with reference to the influence exerted by steady illumination on the overall-level of retinal maintained "dark" discharge.

The muscle activity spectrum: spectral analysis of muscle force as an estimator of overall motor unit activity

Starting from the observation that the part above 6 Hz of the power spectrum of force tremor during isometric contractions can be related to the unfused twitches of motor units firing asynchronously, an attempt was made to study the usefulness of force tremor spectral analysis as a global descriptor of motoneurone pool activity. To compensate for the mechanical low-pass filter characteristic of skeletal muscle which leads to increased damping of mechanical ripples at higher frequencies, a numerical compensation rule was derived from data obtained by electrical microstimulation of small ensembles of motor units (MUs). Hidden line plots of consecutive partially overlapping spectra allowed visualization of changes in spectral composition during ongoing muscle activity. The resulting muscle activity spectra (MAS) showed broad peaks according to the range of onset firing rates in normal subjects. These tended to shift to higher frequencies with increasing force. Under conditions with increased synchronization of the MUs these broad peaks were replaced by sharp peaks corresponding to the burst repetition rate. Patients with different motor dysfunctions were selected to illustrate how alterations of MU activity are reflected in the MAS. Decreased or increased firing rates were observed as well as abnormal states of synchronization. It is concluded that the MAS provides useful information about some aspects of the discharge characteristics of ensembles of MUs and therefore represents a method to monitor some qualitative ‘image’ of the MU activities within a muscle.