Changes In Salivary Cortisol Research Articles

Decreased cortisol among hikers who preferentially visit and value biodiverse riparian zones

While outdoor recreationists often report increases to their well-being for time spent in nature, the mechanisms through which local ecologies affect human health have been difficult to quantify, and thus to manage. We combine data from pre-post salivary cortisol measures, GPS tracks, visitor photos, and surveys from 88 hikers traversing several types of landscape within peri-urban public lands in southwest Idaho, USA. We find that time in biodiverse riparian areas and areas of perceived aesthetic value correlates with decreases in salivary cortisol and improved well-being for hikers. Wildlife sightings were not associated with changes in salivary cortisol, but were associated with riparian travel and aesthetic preferences, indicating an indirect pathway for ecosystem services. Additionally, wildlife sightings decreased on high-use days, even though hikers did not perceive a negative impact of their recreational activity. These results suggest that cultural and physiological ecosystem services of nature depend on the ecological community of the area. Preferential visitation and high service value of riparian areas by hikers and wildlife alike target shared riparian areas as hot spots for management efforts to promote both ecological and human health within an increasingly urbanizing world.

Dynamic Changes in Salivary Cortisol and Protein among Dental Students

Background: Student assessments are the traditional methods of assessing academic success, and they areconsidered to affect one’s career. This study evaluated the levels of salivary stress biomarkers represented bycortisol and total salivary protein during final academic assessments of dental undergraduates.Methods: Saliva samples were obtained, one before the exam another afterwards. Concentrations of salivarystress biomarkers were obtained by enzyme-linked immunosorbent assay (ELISA).Results: Before the exam, the two parameters were dramatically higher than afterwards, with a substantialdifference between the levels of salivary protein and cortisol (p=0.000, 0.000 consecutively).Conclusion: Stress induced by academic examinations may increase the level of salivary stress biomarkersin the short term.

Challenges in Obtaining and Assessing Salivary Cortisol and α-Amylase in an Over 60 Population Undergoing Psychotherapeutic Treatment for Complicated Grief: Lessons Learned.

Biomarkers may serve as objective measures in complicated grief (CG) potentially capturing responses to stress reduction treatment. This paper reports challenges in obtaining and assessing salivary cortisol and α-amylase (sAA) for a recent randomized clinical trial. Within-session changes in salivary cortisol and sAA for 54 older adults with CG who received Accelerated Resolution Therapy were compared with perceived stress measured by Subjective Units of Distress Scale. Bivariate correlations and multiple regressions examined changes in biomarkers. Protocols, study logs, and audit reports identified challenges. Challenges included obtaining unstimulated passive drool salivary samples and their analyses. Our sample of older females on multiple medications may have resulted in a perfect storm of moderating and intervening variables which affected the stress response. This paper contributes to the discussion on designing clinical trials for older adults which must account for physiologic changes, multimorbidity, and polypharmacy common in this population and makes recommendations moving forward.

Effects of mindfulness-based stress reduction on psychological distress in health workers: A three-arm parallel randomized controlled trial

Mindfulness-based Stress Reduction (MBSR) has shown good efficacy for improving wellbeing in employees experiencing occupational stress. However, comparisons with other interventions, longer-term follow-up, and data from varying sociocultural contexts are lacking. This three-arm, parallel randomised controlled trial (RCT) examined the effects of MBSR on psychological distress in non-physician health workers in direct contact with patients. 105 participants were randomly allocated to either: (1) MBSR (N = 35), (2) Stress Management Course (SMC; N = 34) or (3) wait-list (N = 36). Participants and those assessing outcomes were blinded to group assignment. Participants completed questionnaires pre- and post-intervention and four months after the intervention. Psychological distress was measured using the General Health Questionnaire (GHQ-12) and Outcome Questionnaire (OQ-45). Secondary outcomes included perceived stress, job satisfaction, mindfulness skills and changes in salivary cortisol. 77 participants completed measures post-intervention and 52 at 4-month follow-up. MBSR showed a post-intervention effect in reducing GHQ-12 (ß = −0.80 [SE = 1.58] p < 0.01) and OQ-45 (ß = −0.72, [SE = 5.87] p < 0.05) psychological distress, compared to SMC and in reducing GHQ-12 (ß = −1.30 [SE = 1.38] p < 0.001) and OQ-45 (ß = −0.71, [SE = 5.58] p < 0.01) psychological distress compared to wait-list condition. In our secondary outcome, only MBSR was associated with a decrease in the cortisol awaking response by 23% (p < 0.05). At follow-up, only effects of MBSR on the psychological distress ‘social role’ subscale (ß = −0.76 [SE = 1.31] p < 0.05) remained significant, compared to SMC. In conclusion, MBSR appears useful in reducing short-term psychological distress in healthcare workers, but these effects were not maintained at follow-up. Trial registration: ISRCTN12039804.

The effects of acute work stress and appraisal on psychobiological stress responses in a group office environment

BackgroundThe high prevalence of office stress and its detrimental health consequences are of concern to individuals, employers and society at large. Laboratory studies investigating office stress have mostly relied on data from participants that were tested individually on abstract tasks. In this study, we examined the effect of psychosocial office stress and work interruptions on the psychobiological stress response in a realistic but controlled group office environment. We also explored the role of cognitive stress appraisal as an underlying mechanism mediating the relationship between work stressors and the stress response. Methods and MaterialsNinety participants (44 female; mean age 23.11 ± 3.80) were randomly assigned to either a control condition or one of two experimental conditions in which they were exposed to psychosocial stress with or without prior work interruptions in a realistic multi-participant laboratory setting. To induce psychosocial stress, we adapted the Trier Social Stress Test for Groups to an office environment. Throughout the experiment, we continuously monitored heart rate and heart rate variability. Participants repeatedly reported on their current mood, calmness, wakefulness and perceived stress and gave saliva samples to assess changes in salivary cortisol and salivary alpha-amylase. Additionally, cognitive appraisal of the psychosocial stress test was evaluated. ResultsOur analyses revealed significant group differences for most outcomes during or immediately after the stress test (i.e., mood, calmness, perceived stress, salivary cortisol, heart rate, heart rate variability) and during recovery (i.e., salivary cortisol and heart rate). Interestingly, the condition that experienced work interruptions showed a higher increase of cortisol levels but appraised the stress test as less threatening than individuals that experienced only psychosocial stress. Exploratory mediation analyses revealed a blunted response in subjective measures of stress, which was partially explained by the differences in threat appraisal. DiscussionThe results showed that experimentally induced work stress led to significant responses of subjective measures of stress, the hypothalamic-pituitary-adrenal axis and the autonomic nervous system. However, there appears to be a discrepancy between the psychological and biological responses to preceding work interruptions. Appraising psychosocial stress as less threatening but still as challenging could be an adaptive way of coping and reflect a state of engagement and eustress.

0265 Cortisol and C-Reactive Protein Fail to Predict Individual Differences in Neurobehavioral Performance Responses to Total Sleep Deprivation and Psychological Stress

Abstract Introduction Individuals show marked differential vulnerability in neurobehavioral deficits from psychosocial stress and sleep deprivation. Although changes in salivary cortisol and C-reactive protein (CRP) typically occur across total sleep deprivation (TSD) and recovery sleep, whether these biological markers during fully rested conditions predict individual differences in cognitive performance during TSD and stress remains unknown. Methods Thirty-one healthy adults (ages 27–53; mean ± SD, 35.4 ± 7.1y; 14 females) participated in a five-day experiment consisting of two 8h time-in-bed (TIB) baseline nights, followed by 39h TSD, and two 8h-10h TIB recovery nights. A modified Trier Social Stress Test (TSST) was conducted on the day of TSD to induce psychological stress. Salivary cortisol and CRP from blood were obtained at six time points during the study (pre-study, baseline, during TSD, during TSD after the TSST, after recovery, and post-study). A median split of TSD performance [total lapses (&amp;gt;500 ms response time) and errors] on the 10-minute Psychomotor Vigilance Test (PVT) defined cognitively resilient (n=15) and cognitively vulnerable (n=16) groups. Repeated measures ANOVA and post-hoc comparisons corrected for multiple testing, examined cortisol and CRP across time points between groups. Results In both cognitively resilient and vulnerable individuals, cortisol increased with TSD compared to baseline in the morning and decreased with TSD + psychological stress in the afternoon compared to TSD alone. By contrast, there were no significant changes in CRP levels throughout the experiment. In addition, there were no significant time*group interactions in cortisol or CRP levels. Conclusion Salivary cortisol increased with TSD compared to baseline and showed a time-of-day effect with stress during TSD. Notably, cortisol and CRP did not differ between cognitively resilient and vulnerable individuals across TSD, psychological stress or recovery sleep and thus are not reliable biomarkers for predicting performance under these conditions. Support NASA NNX14AN49G.

Changes in human health parameters associated with an immersive exhibit experience at a zoological institution.

Zoological institutions often use immersive, naturalistic exhibits to create an inclusive atmosphere that is inviting for visitors while providing for the welfare of animals in their collections. In this study, we investigated physiological changes in salivary cortisol and blood pressure, as well as psychological changes among visitors before and after a walk through the River’s Edge, an immersive, naturalistic exhibit at the Saint Louis Zoo. Study participants had a significant reduction in salivary cortisol and blood pressure after walking through the exhibit. Psychological assessments of mood found that most visitors felt happier, more energized, and less tense after the visit. Additionally, participants who spent more time in River’s Edge, had visited River’s Edge prior to the study, and had seen more exhibits at the Zoo prior to entering River’s Edge experienced greater psychological and/or physiological benefits. We conclude that immersive, naturalistic exhibits in zoos can elicit positive changes in physiological and psychological measures of health and well-being and argue for a greater scientific focus on the role of zoos and other green spaces in human health.

Outcomes of collage art-based and narrative self-expression among home hospice caregivers

ABSTRACT Aims: This mixed methods quasi-experimental study examined the outcomes of a brief art therapy-based and a narrative interview-based intervention to address the psychosocial needs of home hospice caregivers. Methods: Participants (n = 14) chose to participate either in a narrative interview (n = 7) or arts-based interview (n = 7). Standardised self-report measures of affect, spiritual well-being, perceived stress, and self-efficacy and physiological markers of stress(salivary cortisol) and social/emotional connection (oxytocin levels) were collected before and after intervention sessions. Results: Qualitative findings indicated that caregivers (a) recognized their need for reducing stress and isolation; (b) experienced a learning curve related to illness and hospice care; (c) engaged in existential reflections on purpose and values; and were (d) living through progressive grief and loss. Quantitative findings indicated that the narrative condition group experienced improved spiritual well-being and negative affect. Both conditions were followed by improved positive affect. There were no statistically significant changes in salivary cortisol or oxytocin for either condition. Conclusion: The results suggest that caregivers might need longer term interventions for sustained changes. Implications for further research: Further research is needed to examine the role of narrative and visual self-expression among home hospice caregivers. Study limitations included a small non-randomised sample, lack of transcribed interview data, and incomplete survey and biomarker data. Plain-language summary This study examined the outcomes of an art therapy-based and a narrative intervention to address the mental, physical, and spiritual needs of family caregivers of patients at the end of life. Seven caregivers completed a therapeutic narrative interview in which caregivers shared their experiences, challenges, and sources of strength. Seven caregivers completed a collage art- based session in which they were invited to make a collage using a range of precut magazine collage images while sharing their experiences, challenges, and sources of strength as caregivers. Measures of stress (cortisol) and social/emotional connection (oxytocin) were collected before and after intervention sessions. Findings indicated that caregivers appreciated the opportunity for self-expression in both the narrative and the art-based conditions. Caregivers in the narrative condition group trended toward improvement in spiritual well-being and in reversing negative mood. Both conditions indicated a trend toward improved positive mood. Findings indicated that caregivers were consistently stressed and often isolated due to their roles. Four recurring themes were found within the qualitative data for caregivers in both conditions: (a) recognition and reduction of stress and isolation; (b) learning curve related to illness and hospice care; (c) existential reflections on death and living out values; and (d) living through progressive grief and loss. The preliminary quantitative findings suggest that narrative therapeutic approaches might be better suited for spiritual well-being. Caregivers in the narrative condition group demonstrated more improvement in spiritual well-being than those in the arts-based condition group. This finding may be the result of the format of the narrative condition in which caregivers reflected on existential concerns. In contrast, the structure of the art-making condition may have resulted in a focus on the present moment.

Children’s Diurnal Change in Salivary Cortisol in Relation to Temperament, Peer Relationship and Teacher-Child Relationship: Differences according to Childcare Duration

Children’s Diurnal Change in Salivary Cortisol in Relation to Temperament, Peer Relationship and Teacher-Child Relationship: Differences according to Childcare Duration

Effect of urban trips on stress and cognitive performance, a study in Bogotá, Colombia

IntroductionUrban commuting can be a stressful experience and may impact cognitive function, a key component of overall health. The purpose of this study was to explore whether the cognitive performance of adults is differentially affected by stress resulting from commuting to study by public transportation or by bicycle in Bogotá, Colombia. MethodsWe designed an experiment in which stress and cognitive performance were systematically measured in seven participants, in triplicate, on separate days, before and after a trip by either public transportation or bicycle (total 6 trips). All participants were asked to complete a Stroop test and provide saliva samples before and after the trips. After-versus-before trip differences in salivary cortisol, weighted time-Stroop and percentage of success in the Stroop were compared between modes by a two-way analysis of variance (ANOVA) using mode of transport and participant as fixed factors. ResultsThe mean change in adjusted Stroop time was similar between modes (1,392±8,379 for bicycle, 889 ms ± 6,675 ms for public transport, p = 0.85). The same occurred with the mean change in the percent of success in the Stroop test (1.39% ±5.16% for bicycle, −0.59±5.79% for public transport, p = 0.83). For percent successes in the Stroop, the p-value for the participant*mode term was p = 0.051. The mean change in salivary cortisol was −0.39±1.69 ng/mL for bicycle and −1.02± 1.97 ng/mL for public transport (p = 0.11). The term associated with inter-individual variability in change in cortisol was significant (p˂0.001). ConclusionsCommuting by bike or public transport induced a comparable effect on biological stress and cognitive performance. It is well-known that biking imparts many positive societal and health effects. Our results additionally show that despite involving physical effort, biking does not induce a larger biological stress response than public transportation.

Associations Between Cardiorespiratory Fitness and Endocrine, Autonomous and Psychological Stress Reactivity in Male Adolescents

Abstract. High stress burden during adolescence can have severe long-term health consequences. While some studies reported that adults with higher fitness levels show lower stress reactivity, research on adolescents is scarce. Accordingly, the aim of the present study was to investigate the association between cardiorespiratory fitness and physiological and psychological stress reactivity in male adolescents. Forty-three healthy, male adolescents aged 16–20 years underwent the Physical Working Capacity 170 bicycle ergometer test to determine cardiorespiratory fitness. The Trier Social Stress Test (TSST) was used to trigger a stress reaction, which was measured physiologically with changes in salivary cortisol and alpha-amylase concentrations, and psychologically using self-rated changes in state-anxiety. Under consideration of potential confounders, hierarchical regression analyses were calculated for each outcome. For cortisol and psychological stress reactivity, fitness did not significantly explain variance. However, 28% of variance in alpha-amylase reactivity were explained by fitness and sleep complaints [adjusted R2 = .28, F(2, 36) = 8.36, p = .001], with 16% of variance explained by fitness alone (β = −.41, p = .006). Accordingly, higher fitness was associated with lower stress reactivity of the autonomous nervous system in male adolescents. The promotion of cardiorespiratory fitness may therefore be considered an important factor in preventing negative health consequences of stress in this age group.

Взаимосвязь между гемодинамическими реакциями на холодовой стресс и изменениями уровня саливарного кортизола

An experimental study was conducted with the participation of 30 healthy male volunteers (18–20 years) in order to determine the types of hemodynamic reactions to the general cooling of the body and to determine the severity of cold stress from the dynamics of salivary cortisol levels. The experiment included three 10-minute stages: rest t +20 oС; being in a cold chamber t –20 oС; warming t +20 oС. During each stage, blood pressure (BP), heart rate (HR), temperature in the ear canal were determined, and saliva samples were taken for subsequent determination of free cortisol levels. Volunteers were divided into two groups according to the dynamics of changes in salivary cortisol during cooling: with an increase (n = 22) and a decrease (n = 8) in the hormone’s level. It was shown that during cooling the body temperature of the subjects significantly decreased by an average of 2.1–2.3 oС and did not reach the initial values by the end of the warming stage. In both groups of individuals, when cooling, an increase in BP and a decrease in HR occurred, while in the group with an increase in cortisol, significantly higher levels of systolic BP and a slight decrease in HR were detected. During warming, the hemodynamic indices tended to the initial levels, and the change in the salivary cortisol level was similar to that during cooling. Thus, an increase in the level of salivary cortisol during short-term (10 min) cooling of the human body is associated with severe cold arterial hypertension, a lower baroreflex response, and the risk of cold damage to blood vessels. A decrease in salivary cortisol as a reflection of a decrease in the release of free hormone into the cell during cold stress, in combination with moderate cold arterial hypertension, may indicate a successful adaptation of the body to cold with limited development of hypothermia.

Increasing Dietary Carbohydrate as Part of a Healthy Whole Food Diet Intervention Dampens Eight Week Changes in Salivary Cortisol and Cortisol Responsiveness.

It is largely unknown whether and how whole food diets influence psychological stress and stress system responsiveness. To better understand the effects of whole diets on stress system responsiveness, we examined randomized control trial effects of a whole food diet based on the Dietary Guidelines for Americans (DGA) on cortisol responsiveness. A randomized, double-blind, controlled 8-week intervention was conducted in overweight and obese women to examine differentiated effects between two diet intervention groups: one based on the 2010 DGA and the other one based on a typical American diet (TAD). During a test week that occurred at baseline and again after 8 weeks of the intervention, we assessed salivary cortisol collected at 14 selected times across the day, including upon awakening, at bedtime, and during a test visit, and administered a standardized social stress task (Trier Social Stress Test, TSST). There were no statistical differences between the diet groups in salivary cortisol at baseline or after 8 weeks. However, when considering differences in dietary carbohydrate, but not fat or protein, from the pre-intervention (habitual) to the intervention period, there was a significant (P = 0.0001) interaction between diet group, intervention week, saliva sample, and level of intervention-based change in carbohydrate consumption. This interaction was reflected primarily by an 8-week reduction in salivary cortisol during a period just prior to (log Δ −0.35 ± 0.12 nmol/L) and 30 (log Δ −0.49 ± 0.12 nmol/L), 60 (log Δ −0.50 ± 0.13 nmol/L), 90 (log Δ −0.51 ± 0.13 nmol/L), and 120 (log Δ −0.4476 ± 0.1231 nmol/L) min after the TSST in the DGA group having the highest increase (90th percentile) in carbohydrate consumption. In support of this finding, we also found significant (P < 0.05) and inverse linear associations between dietary carbohydrate and log salivary cortisol, with the strongest negative association (β: −0.004 ± 0.0015, P = 0.009) occurring at 30 min post-TSST, but only in the DGA group and at week 9 of the intervention. Together, increasing dietary carbohydrate as part of a DGA-based diet may reduce circulating cortisol and dampen psychological stress-related cortisol responsiveness.

A Multi-Factorial Assessment of Elite Paratriathletes' Response to Two Weeks of Intensified Training.

In able-bodied athletes, several hormonal, immunological and psychological parameters are commonly assessed in response to intensified training due to their potential relationship to acute fatigue and training/non-training stress. This has yet to be studied in Paralympic athletes. Ten elite paratriathletes were studied for five weeks around a 14-day overseas training camp whereby training load was 137% of pre-camp levels. Athletes provided: six saliva samples (one pre-camp, four during camp, one post-camp) for cortisol, testosterone and secretory immunoglobulin A; weekly psychological questionnaires (POMS and RESTQ-S); daily resting heart rate and subjective wellness measures including sleep quality and quantity. There was no significant change in salivary cortisol, testosterone, cortisol:testosterone ratio or secretory immunoglobulin A during intensified training (p≥0.090). Likewise, there was no meaningful change in resting heart rate or subjective wellness measures (p≥0.079). Subjective sleep quality and quantity increased during intensified training (p≤0.003). There was no significant effect on any POMS subscale other than lower anger (p=0.049) whilst there was greater general recovery and lower sport and general stress from RESTQ-S (p≤0.015). There was little to no change in parameters commonly associated with the fatigued state which may relate to the training camp setting minimising external life stresses and the careful management of training loads from coaches. This is the first evidence of such responses in Paralympic athletes.

Changes in Salivary Cortisol and Iga Levels are Associated with Fatigue after Acute Endurance Exercise.

Changes in Salivary Cortisol and Iga Levels are Associated with Fatigue after Acute Endurance Exercise.

Febrile seizures and increased stress sensitivity in children: How it relates to seizure characteristics

BackgroundStudies suggest that the relationship between seizures and stress starts early in life. However, evidence of long-term altered stress reactivity following early-life seizures is lacking. Our objectives were to assess alterations in stress hormone reactivity in children with past febrile seizures (FS) and investigate how these alterations relate to clinical characteristics. MethodThis case–control study compared a convenience sample of children with simple FS (n = 24), complex FS (n = 18), and matched healthy controls (n = 42). Stress was induced by electrode placement for an electroencephalography (EEG) exam. Salivary cortisol to stress, using three samples collected before and after the stressor, was compared between groups and sex. The relationship between stress reactivity and clinical characteristics (i.e., FS duration, age at first FS, time since the last FS) was investigated. ResultsCortisol reactivity to stress was significantly different depending on study groups, F(1, 78) = 6.415, p = 0.003, η2p = 0.141, but not sex nor was there a significant interaction between group and sex (p ≥ 0.581). Participants with simple FS showed higher cortisol reactivity to stress (M = 14.936, Standard deviation (SD) = 26.852) compared with those with complex FS (M = −4.663, SD = 18.649, p = 0.015) and controls (M = −3.817, SD = 18.907, p = 0.003). There was no significant difference between participants with complex FS and controls (p > 0.999). Stress reactivity was not linked to clinical characteristics. ConclusionsChildren with past simple FS showed greater changes in salivary cortisol following stress, suggesting enhanced stress sensitivity. As similar results were not found in a population with complex FS, our study shows that stress alterations are not caused by seizure severity. Future studies are needed to investigate whether stress sensitivity may be premorbid to simple FS and may contribute to simple FS incidence.

Exploration of psychological mechanisms of the reduced stress response in long-term meditation practitioners

Previous research links contemplative practices, such as meditation, with stress reduction. However, little is known about the psychological mechanisms underlying this relationship. This study compares the physiological stress response (reactivity and recovery) measured by changes in salivary cortisol, heart rate, heart rate variability, and the associated stress-related ratings in long-term meditation practitioners (N = 29) and age- and sex- matched meditation naïve controls (N = 26). The participants were administered the Trier Social Stress Test in its active and placebo versions. The results demonstrated that long-term meditation practitioners had faster cortisol recovery from stress, and experienced less shame and higher self-esteem after the exposure to social-evaluative threat. In addition, long-term meditation practitioners scored higher on adaptive cognitive emotion regulation strategies, such as acceptance and positive reappraisal, and lower on maladaptive ones, such as catastrophizing. The cognitive emotion regulation strategy of acceptance mediated the relationship between meditation practice and cortisol recovery. These results suggest that meditation practice is associated with faster recovery from stress due to the employment of adaptive emotion regulation strategy of acceptance, delineating a pathway underlying the positive effects of meditation on stress.

Multi-modal pain assessment: are near-infrared spectroscopy, skin conductance, salivary cortisol, physiologic parameters, and Neonatal Facial Coding System interrelated during venepuncture in healthy, term neonates?

BackgroundImproving pain and stress assessments in neonates remains important in preventing the short- and long-term consequences. We aimed to identify the relationships between different pain assessment parameters by simultaneously measuring changes in cortical, autonomic, hormonal, physiological, and behavioral evoked responses to venepuncture in healthy, full-term neonates.MethodsThis observational, prospective study (ancillary to the ACTISUCROSE trial) included 113 healthy, 3-day old, full-term neonates who underwent venepuncture for systematic neonatal screening, from July to October 2013, in a tertiary-level maternity ward of a university hospital. During venepuncture, we simultaneously measured the cortical single-channel near-infrared spectroscopy (NIRS) signals, foot skin conductance, salivary cortisol, physiological responses, and behavioral (Neonatal Facial Coding System [NFCS]) evoked responses.ResultsRegarding the NIRS analysis, the highest correlation was between the NFCS at venepuncture and the change in NIRS integrated values of total hemoglobin (r=0.41, P<0.001) or oxygenated hemoglobin (r=0.27, P<0.001). The NFCS at venepuncture was moderately positively correlated with changes in salivary cortisol (r=0.42, P<0.001) and skin conductance (r=0.29, P<0.001). Salivary cortisol and skin conductance changes were not correlated; the latter parameters were not correlated with heart rate, respiratory rate, or SpO2.ConclusionDuring venepuncture, NFCS was mildly or moderately correlated with salivary cortisol, skin conductance, and cortical NIRS changes.

Differences in salivary hormones and perception of exertion in elite women and men volleyball players during tournament.

Sports tournaments induce both psychological and physiological stress, which seems to be different between men and women. Competition induces anticipatory rises in testosterone and cortisol levels, which may affect performance and physical exertion during tournaments. The aim of this study was to compare the changes in salivary cortisol and testosterone concentrations between men and women during an official volleyball tournament and to test potential correlations between changes in these hormones and perceived exertion after match. Three matches of each team were assessed in the group stage of the Men and Women South American Volleyball Championship. Salivary cortisol and testosterone levels were measured in the fasting state, before and after each match. The rate of perceived exertion (RPE) was assessed after each match. Fasting cortisol concentrations were higher in women than men (~25%, P<0.001) while fasting testosterone was higher in men than women (~46%, P<0.001). Cortisol concentration increased only after the second match in men (+53.7%, P<0.001). Testosterone concentration was low before and after the third match in men (P<0.001) while it was elevated after the third match in women (P=0.003). The rate of perceived exertion was correlated with the change in testosterone levels due to the matches in both women (r=0.33; P=0.04) and men (r=0.44; P=0.02), which was not observed for cortisol concentrations. These results indicate that women have higher fasting cortisol, but lower fasting testosterone concentrations than men during a volleyball tournament. Thus, hormonal responses of women and men are different and related to their effort during the matches.

The socially evaluated handgrip test: Introduction of a novel, time-efficient stress protocol

Most widely-used stress-induction procedures (such as the TSST and the Cold Pressor Test) require considerable effort and overhead in terms of preparation, logistics, and staff recruitment. Moreover, while known to reliably induce HPA axis activation, especially when combined with social self-threat, most conventional laboratory stressors cannot be flexibly adapted to elicit either a mainly autonomic or an additional endocrine stress response. Being a promising alternative approach, a new version of the isometric handgrip test enriched by a social-evaluative component was evaluated in the present study.On two consecutive sessions, forty participants (20 women) performed a handgrip task at both 45% (stress) and 10% (control) of maximum voluntary isometric contraction lasting for 3min. During the stress test, continuous visual feedback on performance was given. Participants in the social-evaluative condition (50%) were observed and evaluated by a previously unknown person of the opposite sex, whereas in the standard condition feedback was provided via a computer monitor. Cardiovascular measures (heart rate, blood pressure) as well as additional indices of autonomic reactivity (skin conductance, heart-rate variability) were registered before, during, and after stress induction. Moreover, changes in salivary cortisol and in subjective well-being were assessed.Relative to control, significant increases in cardiovascular and sympathetic activity were found, irrespective of experimental group. Importantly, however, additional social evaluation resulted in elevated cortisol levels. Furthermore, evidence for reduced vagal tone during sustained socially evaluated handgrip emerged. In conclusion, the socially evaluated handgrip test represents a versatile, time-efficient method to induce stress in small laboratory settings.