Changes In Nitrate Concentrations Research Articles

Comprehensive Proteome Profiling of a Xanthomonas campestris pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis.

Xanthan, a bacterial polysaccharide, is widespread in industrial applications, particularly as a food additive. However, little is known about the process of xanthan synthesis on the proteome level, even though Xanthomonas campestris is frequently used for xanthan fermentation. A label-free LC-MS/MS method was employed to study the protein changes during xanthan fermentation in minimal medium. According to the reference database, 2416 proteins were identified, representing 54.75 % of the proteome. The study examined changes in protein abundances concerning the growth phase and xanthan productivity. Throughout the experiment, changes in nitrate concentration appeared to affect the abundance of most proteins involved in nitrogen metabolism, except Gdh and GlnA. Proteins involved in sugar nucleotide metabolism stay unchanged across all growth phases. Apart from GumD, GumB, and GumC, the gum proteins showed no significant changes throughout the experiment. GumD, the first enzyme in the assembly of the xanthan-repeating unit, peaked during the early stationary phase but decreased during the late stationary phase. GumB and GumC, which are involved in exporting xanthan, increased significantly during the stationary phase. This study suggests that a potential bottleneck for xanthan productivity does not reside in the abundance of proteins directly involved in the synthesis pathways.

Application of a portable ion chromatograph for real-time field analysis of nitrite and nitrate in soils and soil pore waters

Real-time monitoring of nitrite and nitrate is crucial for maintaining soil health and promoting plant growth. In this study, a portable ion-chromatograph (IC, Aquamonitrix) analyser, coupled with a field-applicable ultrasonic-assisted extraction method, was utilised for in-field determination of nitrate and nitrite in soils. This is the first application of this type of analyser to soil nutrients. On-site analysis of soil from a local sports field showed 94.8 ± 4.3 μg g−1 nitrate, with LODs of 32.0 μg g−1 for nitrate and 5.4 μg g−1 for nitrite. The results were in close agreement with those obtained using a conventional lab-based IC. Relative standard deviations (%RSDs) for soil analysis using Aquamonitrix were consistently below 10%. The obtained average recoveries of samples spiked with nitrite were 100% and 104% for the portable IC and conventional IC, respectively. Furthermore, to assess the suitability of portable IC for samples with high organic matter content, various natural organic fertilisers were extracted and analysed. The results showed 16.2 ± 0.7 μg g−1 nitrite and 28.5 ± 5.6 μg g−1 nitrate in sheep manure samples with LODs of 2.0 μg g−1 for nitrite and 12.0 μg g−1 for nitrate. The portable IC system was further demonstrated via real-time on-site analysis of soil pore-water acquired using a portable battery-based ceramic pore-water sampler. A continuous increase in nitrate concentration over time was observed (from 80 to 148 μg mL−1) in the soil pore-water in a vegetable garden four days after heavy rain. Unlike conventionally sampled natural waters, 7-day storage of the studied pore water samples revealed no changes in nitrate concentrations. An average of 558 ± 51 μg mL−1 nitrate was detected in the soil pore-water samples analysed on a spinach farm, immediately after irrigation.

Human skeletal muscle nitrate and nitrite in individuals with peripheral arterial disease: Effect of inorganic nitrate supplementation and exercise.

Skeletal muscle may act as a reservoir for N-oxides following inorganic nitrate supplementation. This idea is most intriguing in individuals with peripheral artery disease (PAD) who are unable to endogenously upregulate nitric oxide. This study analyzed plasma and skeletal muscle nitrate and nitrite concentrations along with exercise performance, prior to and following 12-weeks of exercise training combined with oral inorganic nitrate supplementation (EX+BR) or placebo (EX+PL) in participants with PAD. Non-supplemented, at baseline, there were no differences in plasma and muscle nitrate. For nitrite, muscle concentration was higher than plasma (+0.10nmol.g-1 ). After 12 -weeks, acute oral nitrate increased both plasma and muscle nitrate (455.04 and 121.14 nmol.g-1 , p < 0.01), which were correlated (r=0.63, p < 0.01), plasma nitrate increase was greater than in muscle (p < 0.01). Nitrite increased in the plasma (1.01 nmol.g-1 , p < 0.05) but not in the muscle (0.22 nmol.g-1 ) (p < 0.05 between compartments). Peak walk time (PWT) increased in both groups (PL + 257.6s;BR + 315.0s). Six-minute walk (6MW) distance increased only in the (EX+BR) group (BR + 75.4m). We report no substantial gradient of nitrate (or nitrite) from skeletal muscle to plasma, suggesting a lack of reservoir-like function in participants with PAD. Oral nitrate supplementation produced increases in skeletal muscle nitrate, but not skeletal muscle nitrite. The related changes in nitrate concentration between plasma and muscle suggests a potential for inter-compartmental nitrate "communication". Skeletal muscle did not appear to play a role in within compartment nitrate reduction. Muscle nitrate and nitrite concentrations did not appear to contribute to exercise performance in patients with PAD.

Comparative analysis of nitrate evolution patterns during pollution episodes: Method development and results from Tianjin, China

Particulate nitrate plays an increasingly important role in the formation of air pollution process, while the main mechanisms of nitrate concentration change are different in each stage, same as the driving factors. In this study, we proposed an episode-based analysis to illustrate the typical nitrate evolution patterns and identify the possible impacting factors in different evolution stages. Applying into twelve air pollution episodes, three typical patterns of nitrate evolution were abstracted, and the corresponding conceptual models were constructed. All the pollution episodes were grouped by their evolving shapes, which were driven by physical and chemical processes. Episodes started slowly typically arose from gradual pollutant accumulation, both locally and regionally, and chemical formation under high humidity. Type 1 (“hump-shaped type”), accounting for 66.3 % of the total episode durations, including two “peak” concentrations, displays a rapid growth rate which could up to 4.6 μg m−3 h−1 in average, mainly relying on the sharp drop in the planetary boundary layer height. Short scavenging processes and thoroughly dissipated stages of the pollution episodes always accompanied by strong north wind affected by Siberia-Mongolia cold current. Type 2 (“triangle-shaped type”, 24.3 %) shows a gentle growth rate and short duration. Compared with Type 1, chemical process may be more important “source” for the increase of nitrate concentration during Type 2. Type 3 (“trapezoid-shaped type”, 9.4 %) presents a long platform stage, during which high humidity (RH > 90 %) provides favorable conditions for wet removal and secondary production, and the updraft can carry pollutants to high altitude. The source and sink are roughly balanced for Type 3. Our study highlights the importance of pattern identification for understanding the nitrate evolution behavior, it may also provide insights for pollution prediction and scientific mitigation strategies.

Source apportionment of nitrates in different aquifers in an arid region, northwestern China

The nitrate pollution of groundwater in arid areas subject to human activity, and especially agricultural activity, is of immense environmental concern. Groundwater is the main water source in arid areas, once an aquifer is polluted, the impact upon the security of the water supply becomes all the more serious. The process of nitrate transformation is also different from that of humid areas, therefore, it is very important to identify and quantify the nitrate sources in aquifers. Using the hydrochemical, stable isotope tracers of 194 groundwater samples, and combining these with the MixSIAR model, the spatial distribution of groundwater nitrates in the Shiyang River Basin (SRB), China, was mapped. The relative contributions made by nitrate sources to different aquifers, and their key transformation processes, were identified. It was found that, shallow groundwater is seriously polluted, with NO3− concentrations ranging from 1.1 to 438.7 mg L−1. In the SRB, NO3− concentrations of 23% and 73% in shallow groundwater samples exceed the NO3− concentration limits found in natural sources (13.3 mg L−1), and the World Health Organization (WHO) nitrate limits for drinking water (50 mg L−1). Furthermore, the nitrates found in confined aquifers indicate that these are also affected by anthropogenic sources, with concentrations ranging from 1.6 to 44.7 mg L−1. Soil organic nitrogen (51.9%) and NH4+ fertilizer (20.8%), wastewater and manure (20.5%) are the main sources of groundwater nitrates; NO3− fertilizer (4.4%) and precipitation (2.3%) have a relatively insignificant effect on groundwater nitrates. The process of nitrification is the main factor affecting changes in nitrate concentrations in aquifers. Other than the denitrification observed in the shallow aquifers of the Minqin Basin (MQB), there was no significant denitrification in the other sub-basins of the SRB. Anthropogenic sources, especially agricultural sources, are now major contributors to the increases in groundwater nitrate concentrations observed in the SRB. This study provides data that can be used to help prevent groundwater pollution and enhance water resources management in arid areas.

Preliminary Study Of Nitrate Production By Plasma Discharge As Potential Fertilizer Material

Nitrogen-based fertilizer production mainly involves the Haber-Bosch process, which synthesizes Ammonia via a chemical reaction of N2 from the air and H2 under high temperature and pressure conditions in the presence of a catalyst. However, the environmental concern for greenhouse gas emissions and high energy consumption triggers other Nitrogen fixation method development. The non-thermal plasma oxidation of N2 into Nitrate is a promising method since it has lower energy consumption and a more suitable process condition with a better environmental profile. This study tested the designed plasma discharge reactor tube to produce nitrate from air N2 with varying voltage, time, and airflow rate operating conditions. Nitrate concentration was analyzed using a UV-Vis spectrophotometer. The results show that nitrate concentration changes linearly with the applied voltage in a constant airflow rate and length of treatment time. The highest performance of the plasma discharge reactor was achieved on a 6 kV applied voltage with a flow rate of 0.2 L/min and a treatment time of 40 minutes where the obtained nitrate concentration was 7.7 ppm, which indicated the potential plasma discharged nitrate production for green fertilizer application. Further study shall be carried out to enhance the performance.

Modeling Monthly Nitrate Concentration in a Karst Spring with and without Discrete Conduit Flow

Understanding the coupled continuum pipe-flow framework for modeling contaminant transport in karst systems is critical for protecting water resources therein. This study simulated point and non-point source nitrate concentration in a karst spring and investigated the results generated from the flow and transport model with and without discrete conduit flow. CFPv2 and CMT3D models were integrated to address the changes in nitrate concentration at a monthly scale in a karst spring, and the results were compared with that from an equivalent porous media (EPM) model with high-hydraulic conductivity (K) zones set in the main karstified area to represent conduits. The results show that the CFPv2+CMT3D model is able to describe well the recession of nitrate concentration in spring discharge, and the relatively larger deviation (slower nitrate recession) from the observed trend for the EPM model is probably a result of the limitation of utilizing high hydraulic conductivity cells to represent conduit. Moreover, simulated hydraulic heads in poorly karstified areas from the two models both show slight differences from the observations (the head RMSE values of calibration/validation for CFPv2 and MODFLOW models are 0.16 m/0.25 m and 0.26 m/0.17 m, respectively), indicating the inclusion of conduits may not affect the simulation considerably, and the lower the proportion of karstic area, the slight effects brought from the inclusion of conduits in the model. For highly karstified areas, the CFPv2+CMT3D model could provide more accurate results (head RMSE of calibration/validation for CFPv2 and MODFLOW are 0.22 m/0.06 and 0.52 m/0.47 m, respectively), showing the coupled continuum pipe-flow framework may be more appropriate for applying to highly and maturely karstified areas where the variations in the behavior of flow and contaminant transport are more affected by turbulent flow regime.

Electrodialysis ion-exchange membrane bioreactor (EDIMB) to remove nitrate from water: Optimization of operating conditions and kinetics analysis

Nitrate pollution has become a worldwide problem. In this study, we remove nitrate from water by electrodialysis ion-exchange membrane bioreactor (EDIMB) and enabling simultaneous nitrate enrichment and denitrification. In this reactor, nitrate migrated from the water chamber to the biological chamber via electrodialysis and was degraded by microorganisms. The effects of voltage and biomass concentration on the reactor performance were examined, and the kinetics data of the water chamber and biological chamber were fitted. The experimental results showed that the migration of nitrate in the water chamber conformed to the first-order model, and the constructed zero-Michaelis-Menten model described changes in nitrate concentration in the biological chamber. Furthermore, when the inflow nitrate concentration was 40 mg N/L, 5 V was the best voltage, and 3.00 g VSS/L was the best biomass concentration. The nitrate removal rate in the water chamber was 98.94%, and there was no accumulation of nitrate or nitrite in the biological chamber. Compared with traditional ED processes, the nitrate removal efficiency was 8.86% higher, and the current efficiency was 22.14% higher. The total organic carbon (TOC) of the water chamber was only 1.43 mg C/L, which proves that the structure of the EDIMB confined the denitrifying bacteria and organic carbon donors in the biological chamber and avoided secondary pollution in the water chamber. Microbial community analysis showed that Thauera (66.06%) was the dominant bacterium in the EDIMB system, and Azoarcus (9.81%) was a minor denitrifying genus.

Effects of heterogeneous reaction with NO2 on ice nucleation activities of feldspar and Arizona Test Dust

Mineral dust is an important type of ice nucleating particles in the troposphere; however, the effects of heterogeneous reactions on ice nucleation (IN) activities of mineral dust remain to be elucidated. A droplet-freezing apparatus (Guangzhou Institute of Geochemistry Ice Nucleation Apparatus, GIGINA) was developed in this work to measure IN activities of atmospheric particles in the immersion freezing mode, and its performance was validated by a series of experimental characterizations. This apparatus was then employed to measure IN activities of feldspar and Arizona Test Dust (ATD) particles before and after heterogeneous reaction with NO2 (10±0.5 ppmv) at 40% relative humidity. The surface coverage of nitrate, θ(NO3−), increased to 3.1±0.2 for feldspar after reaction with NO2 for 6 hr, and meanwhile the active site density per unit surface area (ns) at -20°C was reduced from 92±5 to <1.0 cm−2 by about two orders of magnitude; however, no changes in nitrate content or IN activities were observed for further increase in reaction time (up to 24 hr). Both nitrate content and IN activities changed continuously with reaction time (up to 24 hr) for ATD particles; after reaction with NO2 for 24 hr, θ(NO3−) increased to 1.4±0.1 and ns at -20°C was reduced from 20±4 to 9.7±1.9 cm−2 by a factor of ∼2. Our work suggests that heterogeneous reaction with NO2, an abundant reactive nitrogen species in the troposphere, may significantly reduce IN activities of mineral dust in the immersion freezing mode.

Patterns and Drivers of Groundwater and Stream Nitrate Concentrations in Intensively Managed Agricultural Catchments

The environmental loss of nitrogen in agricultural landscapes has pervasive consequences, including human health implications, eutrophication, loss of habitat biodiversity and greenhouse gas emissions. The efficacy of mitigation strategies designed to control or prevent nitrate contamination of waterbodies requires an understanding of catchment scale pressures and processes. Groundwater and stream nitrate concentrations fluctuate over temporal scales ranging from the daily to the decadal. Identifying spatiotemporal trends and dominant drivers of nitrate in water is challenging as the drivers are intertwined. The effects of agronomic, meteorological and hydrogeological drivers on groundwater and stream nitrate were investigated over seven years in two well-drained agricultural catchments, dominated by tillage and grassland farming, respectively. A significant positive temporal trend in nitrate concentration was observed in the tillage catchment, whereas no long-term trend was observed in the grassland catchment. Agronomic, meteorological and hydrogeological factors were significantly related to temporal nitrate changes across both catchments. Clearly identifying the drivers influencing temporal changes in nitrate concentrations is critical to improving water quality. The study highlighted that to reduce groundwater nitrate levels in areas of high risk (thin soils, low clay content and shallow groundwater), nitrogen applications need to be reduced and/or tailored, particularly at times of restricted crop growth.

Integrated Surface-Groundwater Modelling of Nitrate Concentration in Mediterranean Rivers, the Júcar River Basin District, Spain

High nutrient discharge from groundwater (GW) into surface water (SW) have multiple undesirable effects on river water quality. With the aim to estimate the impact of anthropic pressures and river–aquifer interactions on nitrate status in SW, this study integrates two hydrological simulation and water quality models. PATRICAL models SW–GW interactions and RREA models streamflow changes due to human activity. The models were applied to the Júcar River Basin District (RBD), where 33% of the aquifers have a concentration above 50 mg NO3−/L. As a result, there is a direct linear correlation between the nitrate concentration in rivers and aquifers (Júcar r2 = 0.9, and Turia r2 = 0.8), since in these Mediterranean basins, the main amount of river flows comes from groundwater discharge. The concentration of nitrates in rivers and GW tends to increase downstream of the district, where artificial surfaces and agriculture are concentrated. The total NO3− load to Júcar RBD rivers was estimated at 10,202 tN/year (239 kg/km2/year), from which 99% is generated by diffuse pollution, and 3378 tN/year (79 kg/km2/year) is discharged into the Mediterranean Sea. Changes in nitrate concentration in the RBD rivers are strongly related to the source of irrigation water, river–aquifer interactions, and flow regulation. The models used in this paper allow the identification of pollution sources, the forecasting of nitrate concentration in surface and groundwater, and the evaluation of the efficiency of measures to prevent water degradation, among other applications.

Properties and Use of Water Activated by Plasma of Piezoelectric Direct Discharge

The distilled water was exposed to a cold atmospheric plasma (CAP) generated by the piezotransformer. The treatment was carried out on a six-well plate. Exposure time was from 30 s to 10 min. The emission spectra were obtained. Physical and chemical changes of activated water were observed for 8 days. The reactive oxygen and nitrogen species (RONS) were investigated. RONS production dependence on the exposure time and RONS lifetime in the treated liquid was determined using absorbance spectra. Quantitative assessment was made of the nitrite and nitrate concentration change over time after treatment. The medium conductivity and redox potential varied linearly depending on exposure time. The pH value change correlates with nitrate anion concentration. The concentration of peroxide hydrogen increased depending on CAP water treatment duration. The RONS concentration after liquid media processing with piezoelectric direct discharge CAP is a novel result. Biogeosystem Technique provides CAP activated water agriculture synergetic effect.

Effects of the Japanese 2016 Kumamoto Earthquake on Nitrate Content in Groundwater Supply

The 2016 Kumamoto earthquake had a significant impact on groundwater levels and quality. In some areas, the groundwater level increased significantly due to the release of groundwater from upstream mountainous regions. Conversely, the groundwater level in other areas greatly decreased due to the creation of new fracture networks by the earthquake. There were also significant changes in certain groundwater quality variables. In this study, we used clustering based SOM (self-organizing maps) analysis to improve the understanding of earthquake effects on groundwater quality. We were especially interested in effects on groundwater used for drinking purposes and in nitrate concentration. For this purpose, we studied groundwater nitrate (NO3− + NO2−–N) concentrations for the period 2012–2017. Nitrate concentration changes were classified into seven typical SOM clusters. The clusters were distributed in three representative geographical regions: a high concentration region (&gt;4 mg/L), a low concentration region (&lt;1.6 mg/L) with minimal anthropogenic loading area, and an intermediate concentration region (2–4 mg/L). Depending on these regions, the nitrate concentration changes just before and after the earthquake had both increasing and decreasing trends between 2015–2017. This points to complex physiographical relationships for release of stored upstream groundwater, promotion of infiltration of shallow soil water/groundwater, and nitrate concentration as affected by earthquakes. We present an analysis of these complex relationships and a discussion of causes of nitrate concentration changes due to earthquakes.

Time-delayed photocatalysis enhanced microbial nitrate reduction via solar energy storage in carbon nitrides

The increase in nitrogen, especially nitrate, in the water environment poses a great threat to human health and makes the need to develop ways to efficiently remove nitrate from water. Here, we established a novel time-delayed denitrification system for the first time using dark photocatalyst, the cyanamide-functionalized heptazine-based polymer (NCN-CNx). Interestingly, the dark photocatalysis system can store electrons when being irradiated, and release them as needed when being coupled with a conductive co-catalyst in dark environment. The changes in concentration of nitrate, nitrite and ammonia were measured to state the improved denitrification performance, and the enzyme activities and electrochemical behaviors were investigated to explore the mechanism of the denitrification system. The results showed that NCN-CNx had a positive effect on the microbial nitrate removal process because of the release of stored photogenerated electrons. Electrons released by a co-catalyst (graphene) on demand could promote microbial denitrification with a 77% nitrate removal efficiency, which was almost 4.8 times and 3.1 times higher than that of the photocatalytic group and the microbial group. Correlation analysis indicated that the activities of nitrate reductase, nitrite reductase, and catalase as well as electron transport system were the most important relative factors affected by NCN-CNx during the denitrification process. Furthermore, bacterial community analysis revealed that NCN-CNx increased the relative abundance of denitrifiers, including Sphingomonadaceae, Xanthomonadaceae and Cyclobacteriaceae, in the treated communities compared with the original community in river sludge. These findings provided some new insights into the mechanism by which dark photocatalyst can enhance the denitrification process and the establishment of day/night photocatalytic systems for sustainable removal of nitrate in water.

Modeled Dynamics of Physical and Biological Processes in the Central California Current System From 1993 to 2016

AbstractA three‐dimensional physical‐biological model was used to study the dynamics of physical and biological processes in the central California Current System (CCS) from 1993 to 2016 and investigate its response to different climate modes. The variations of new production (NP) and nitrate fluxes in the central CCS showed robust seasonal and interannual variability. In the coastal upwelling region (0–150 km offshore), the variability of NP was associated with both coastal upwelling and alongshore nitrate advection. In the coastal transition zone (150–300 km offshore), it was related to nitrate fluxes that were determined primarily by the physics rather than nitrate concentration, with vertical mixing and offshore transport being dominant factors. In the offshore oceanic region (California Current; 300–1,000 km offshore), nitrate concentrations played a more important role in the variations of nitrate fluxes, and the NP variability was dominated by the nutrient supplies of vertical mixing and lateral transports. Although El Niño–Southern Oscillation can affect coastal upwelling, alongshore currents, and nitrate fluxes, the coastal NP responded to El Niño–Southern Oscillation mostly in the frequencies of &lt;1 and &gt;6 years. Pacific Decadal Oscillation and North Pacific Gyre Oscillation may affect the CCS ecosystem through large‐scale winds and stratification, modifying the circulation in the upper layer and leading to changes in nitrate concentration and NP.

Nitrate bioreduction dynamics in hyporheic zone sediments under cyclic changes of chemical compositions

Hyporheic zone (HZ) is the hotspot of contaminant biotransformation in ecosystems that affects water quality in both groundwater and river water. HZ is characterized by its dynamic changes in water flow direction that lead to the cyclic changes in aqueous chemical compositions such as dissolved oxygen (DO), nitrate, dissolved organic carbon, and their concentrations. Here we report a study on the effects of cyclic changes in the chemical conditions on the rates of nitrate bioreduction in sediments from a hyporheic zone, and the corresponding dynamics of the synthesis and decay of nitrate reductases. The results indicated that the rate of nitrate bioreduction in the second and third cycles was faster than that in the first one because the nitrate reductases produced in the first cycle still partially maintained their reactivity. The presence of DO significantly decreased the rates of nitrate reduction and nitrate reductase synthesis, but such an inhibition effect diminished in the later cycle. The coupled changes in nitrate concentrations and functional enzymes and genes abundances were generally consistent with an enzyme-based biogeochemical kinetic model. Overall the results indicated that enzyme growth dynamics and functions were complexly affected by the cyclic changes of environmental conditions, and implied that HZ sediments have a memory-type system behavior in reducing nitrate that would be affected by the frequency and magnitude of groundwater and river water exchanges, and chemical compositions in the groundwater and river water.

Utility of ‘Diffusive Gradients in Thin-Films’ for the measurement of nitrate removal performance of denitrifying bioreactors

The increase in environmental nutrient availability as a result of human activities has necessitated the development of mitigation strategies for nutrient removal, such as nitrate. Current methods for determining the efficiency of different mitigation strategies required measurement of changes in nitrate concentrations, however, these methods can be expensive or do not account fully for the temporal variability of nitrate concentration. This study evaluated the utility of Diffusive Gradients in Thins-Films (DGT) for determining nitrate removal in two denitrifying bioreactors, and compared DGT performance to traditional approaches for determining performance, including high and low frequency water grab sampling. The binding layer was produced using the Purolite® A520E anion exchange resin. The uptake and elution efficiencies were 98.8% and 93.4% respectively. DGTs of three material diffusion layer thicknesses were placed in piezometers along longitudinal transects, to enable calculation of the diffusive boundary layer and provide replicates. These were removed after 16, 24 and 36 h, and the accumulated nitrate masses were extracted and quantified to calculate nitrate concentration. Concentrations were subsequently utilised to calculate nitrate removal rates in both bioreactors. Grab samples were taken at 30 and 60 min intervals over those periods, nitrate concentrations were also measured to determine nitrate removal. DGTs provided nitrate removal rates at bioreactor site one (controlled flow, wastewater treatment) of 14.83–30.75 g N m−3 d−1, and 1.22–3.63 g N m−3 d−1 at site two (variable flow, agricultural run-off). DGT determined nitrate concentrations and removal rates were in strong accordance with high frequency grab sampling, but data collection via DGTs was considerably easier. Utilising DGTs for the measurement of bioreactor performance overcame many of the challenges associated with high frequency grab sampling, and other methods, such as accounting for temporal variation in nitrate concentration and reduced analytical requirements.

Bend-induced sediment redistribution regulates deterministic processes and stimulates microbial nitrogen removal in coarse sediment regions of river

Understanding the differences of biogeochemical processes between straight and bent channel is important for weighting them in urban river planning and reconstruction. Shifts in the assembly of the sediment microbial community of bent channels are key, but understudied, component of bend-induced increases in biogeochemical reaction rates. Here, the assembly of microbial community and its feedback to nitrogen transformation in urban river bends were firstly studied by coupling ecological theory, aqueous biogeochemistry, DNA sequencing, and hydrodynamic profiling. It was found that the sediment particle size was the main driving force for producing the significant difference of microbial community structure in river bends. Homogeneous selection, quantified by β-nearest taxon index (βNTI), emerged in the urban river bends and accounted for 79.2% of all ecological processes. Moreover, a significant positive relationship between βNTI and the sediment particle size indicated that shifts in particle size were associated with shifts in deterministic selective pressures, which govern the composition of the microbial community. The significant correlation between the βNTI and changes in nitrate concentration also indicated that nitrate leads to deterministic processes, which select microbial taxa. These microbial taxa which are governed by deterministic processes show specific nitrogen transformation traits, and react on the nitrate concentration. A multiphase transport model allowed the separation of the effects of deterministic processes on nitrogen concentration from measured concentration influenced by complex biogeochemical processes. The results indicated that both the ammonia transformation and microbial nitrogen removal were stimulated in coarse sediment regions of the river bends, and were confirmed by abundant differences of microbial taxa that could promote ammoxidation and denitrification. The coarse sediment benefits microbial nitrogen removal in urban river bends, a discovery that should inform urban river reconstruction designs and the efforts to assess the environmental water capacity of urban rivers.

Assessment of the use of a zero-valent iron permeable reactive barrier for nitrate removal from groundwater in the alluvial plain of the Dagu River, China

Groundwater in a shallow aquifer used for potable supply in Qingdao, China, has been contaminated by nitrate by the excessive use of chemical fertilisers for vegetable production in the area. In this study, a representative site was selected to construct a non-continuous permeable reactive barrier (PRB) back-filled with a mixed reactive medium containing of zero-valent iron, activated carbon and medium- to coarse-grained sand. The PRB consists of alternate well and pillars and was constructed to provide a funnel and gate treatment system for two pumping wells. Groundwater monitoring during a trial of the system indicated that the concentrations of nitrate in monitoring wells within the treatment system rapidly declined (within a day) from about 120 mg/L to 20 mg/L. Reductions in nitrate concentrations were also observed in the pumping wells and downgradient monitoring wells, but changes in nitrate concentrations were moderated by mixing with contaminated groundwater. The PRB construction technique of using alternative wells and pillars combined with jet grouting was found to be effective for nitrate removal from groundwater at a small scale.

Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins

The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse experiments, we further show that this mechanism allows meristems to adapt to rapid changes in nitrate concentration, and thereby modulate their rate of organ production to the availability of mineral nutrients within a few days. Our work sheds light on the role of the stem cell regulatory network by showing that it not only maintains meristem homeostasis but also allows plants to adapt to rapid changes in the environment.