Hematological Changes Research Articles

Sub-chronic Toxicity Study of Heracleumlasiopetalum Extract Towards Healthy Sprague Dawley Rats

Background: Heracleum species are commonly used as spices, flavorings, and food additives. Members of the genus Heracleum offer many medicinal benefits but may also pose adverse effects on human health. Objectives: To prepare a crude leaf extract of Heracleumlasiopetalum and assess its toxicity profile towards healthy rats. Methods: The H. lasiopetalum leaf extract was prepared using pure methanol and ethyl acetate (1:10) at room temperature over a period of 72 hours. After filtration, the crude extract was obtained using a rotary evaporator at 40 - 45°C. Subsequently, various doses of the H. lasiopetalum extract were administered orally to healthy Sprague Dawley rats at three doses (300, 600, and 900 mg/kg body weight) for four weeks to test for toxicity. Blood samples were examined for hematologic and biochemical changes, while the liver, kidneys, and heart were examined for histopathological changes. Results: The toxicity study revealed no mortality at low and medium doses, as well as no clinical toxicity indicators. Additionally, there were no significant alterations observed in the haematological, biochemical, and histopathological profiles of the treated animals throughout the 28-day experiment. However, at high doses, the mortality rate was significantly elevated, accompanied by notable histopathological changes. Conclusions: Continuous administration of high doses of H. lasiopetaum may induce potential toxic effects in the treated animals.

Clinical-Hematological Changes and Predictors of Severity in Acute Food Protein–Induced Enterocolitis Syndrome Reactions at Oral Food Challenge: A Multicenter Observational Study

BackgroundOral food challenge (OFC) is the gold standard for diagnosis of acute Food Protein-Induced Enterocolitis Syndrome (FPIES). No diagnostic/prognostic biomarkers are available, and OFC assessment criteria are not validated. Objective. To assess clinical-haematological changes and predictors of severity of FPIES reactions at OFC. MethodsObservational multicentre prospective study. Children aged 0-18 years diagnosed with acute FPIES were recruited at follow-up OFC in 12 tertiary centres in Spain and Italy. OFC Outcomes (as positive/negative/inconclusive and mild/moderate/severe) were assessed based on published ‘2017 FPIES Consensus’ criteria. Clinical characteristics were recorded, and full blood count was done at baseline, reaction onset and 4 hours later. Regression analysis was performed to assess predictors of severe reactions at OFC. Results81 children had positive OFC (mild in 11% (9/81), moderate in 61% (49/81), severe in 28% (23/81)). Increase in neutrophils and reduction in eosinophils, basophils and lymphocytes was observed (P-value<0.05). OFC was inconclusive in 19 cases despite objective signs or neutrophilia. Regression analysis showed a 2-day OFC protocol where only 25% of an age-appropriate portion is given on day 1 (not gender, age, culprit food, cumulative dose and previous reaction severity) was associated with reduced odds of severe reaction compared to giving multiple doses in a single day. ConclusionDistinct haematological changes may help support FPIES diagnosis. Current OFC assessment criteria may not capture the broad spectrum of acute FPIES presentations. This 2-day protocol may associate a reduced risk of severe reactions. Future work should aim to develop safer OFC and non-OFC diagnostics for FPIES.

Mentha piperita and Thymus vulgaris ameliorate hematological changes induced by chronic consumption of oxidized palm oil in rats

Objective: The aim of this study was to investigate the effect of Mentha piperita L and/or Thymus vulgaris in ameliorating the hematological changes induced by chronic consumption of oxidized palm oil (OPO) in rats. Methodology: Fifty rats were divided into five groups as follows: rats received a basal diet (control), rats received a basal diet sustained with OPO, rats fed OPO and received Mentha piperita extract, rats fed OPO and received Thymus vulgaris extract, and finally rats fed OPO and received both extracts. After 6 weeks, blood samples were withdrawn and a complete blood count test was done. Results: Rats fed a diet containing OPO showed a significant decrease in red blood cells (RBCs), hematocrit (Hct), mean corpuscular volume (MCV), hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), lymphocytes (LYM), and platelets (PLTs) accompanied by a significant increase in white blood cells (WBCs), red cell distribution width (RDW), and mean platelet volume (MPV) compared to rats fed a normal diet. In contrast, rats fed on diet containing OPO and the aqueous extract of either Mentha piperita or Thymus vulgaris showed a significant increase in RBCs, Hct, MCV, Hb, MCH, MCHC, LYM, and PLTs accompanied by a significant decrease in RDW, WBCs, and MPV relative to rats fed on diet containing OPO only. Treatment with a combination of both plants showed better improvements. Conclusion: Both Mentha piperita and Thymus vulgaris ameliorate the hematological changes induced by OPO.

Evaluating the Efficacy of a Polyherbal Formulation in Ameliorating Arthritis Induced by Complete Freund’s Adjuvant

Background: This study investigates the antiarthritic potential of a polyherbal formulation (PHF) comprising extracts from Tinospora cordifolia, Rosa damescena, and Acacia leucoploea in a rat model of rheumatoid arthritis (RA). RA is a chronic autoimmune disorder with debilitating consequences. PHF's impact on joint inflammation, bone degradation, and cartilage preservation were evaluated. Methods: Arthritis was induced using complete Freund’s adjuvant (CFA), and animals were treated with PHF (200 and 400 mg/kg), prednisolone, or control treatments for 28 days. Parameters including body weight, paw volume, arthritis severity score, hematological parameters, serum markers (creatinine, ALP, total proteins), cytokine levels (IL-1β, IL-6, TNF-α, IL-10), and radiographic changes were assessed. Results: CFA-treated rats exhibited significant body weight loss, paw edema, increased arthritis severity scores, altered hematological parameters, and elevated serum markers compared to normal controls. PHF treatment at both doses mitigated body weight loss, reduced paw edema, and improved arthritis severity scores. Hematological changes induced by CFA were also attenuated by PHF treatment. Serum creatinine, ALP, and total protein levels, elevated in CFA-treated rats, were significantly improved by PHF. Furthermore, PHF modulated cytokine levels, decreasing IL-6, IL-1β, and TNF-α while increasing IL-10. Radiographic analysis displayed reduced joint damage in PHF-treated rats compared to CFA controls. Conclusion: This comprehensive investigation highlights PHF's potential to mitigate the inflammatory processes associated with RA, as evidenced by improved clinical, hematological, and biochemical parameters. The study underscores the promise of traditional botanical compounds in managing RA and suggests PHFs as novel therapeutic options. Further mechanistic studies are warranted to elucidate the exact pathways involved.

Specific Immune Marker Associated With Coronavirus Disease 2019

Background: The infection that caused the pneumonia, the virus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the source of coronavirus disease 2019 (COVID-19) is the source of the global pandemic. The pneumonia cases began in Wuhan, China in December 2019 and had no apparent reason. Essential inflammatory mediators Chemokines are necessary for an immune response to eliminate pathogens. However, the fundamental cause of hyperinflammation is their excessive release. Chemokines may be directly responsible for the acute respiratory illness syndrome in the current COVID-19 outbreak. Objective: The current study aimed to estimate hematological changes with immunogenic markers CD177, GNLY, and CXCR4 gene expression in COVID-19 patients, and compare these parameters among patients and healthy individuals. Materials and Methods: The study was conducted at Al-Yarmouk Teaching Hospital and Medical Teaching Laboratories. Finally, to detect the immune markers level in samples of patients, by RT-PCR. Results: The results revealed the gene expression results in patients with COVID-19 means Ct difference of CD177 (24.70) fold more than (10.5) elevated when compared with the mean of Ct control healthy group is (28.21) fold of gene expression is 1.00 of CD177 when our study showed increased gene expression the mean of GNLY Ct receptor was reached to (28.70 ng/L) with fold more than 15.0 in COVID-19 patients, while mean Ct in healthy control was (32.61 ng/L) with fold 1.00, the current study found mean CT of CXCR4 gene expression ( 31.2) with fold (1.1) compared with healthy control CT(31.3) fold of gene expression was (1.0). Conclusion: It was concluded that COVID-19 caused significant changes in many immunological parameters and found the titer of immune markers was higher in infection compared with control.

Impact of Haemoglobin and Haematocrit on the Incidence of Thrombosis in the Individuals Exposed to High-Altitude

Impact of Haemoglobin and Haematocrit on the Incidence of Thrombosis in the Individuals Exposed to High-Altitude

Dynamic changes of hematological and hemostatic parameters in COVID-19 hospitalized patients: Potential role as severity biomarkers for the Chilean population.

COVID-19 is still a global health issue, there is limited evidence in South America regarding laboratory biomarkers associated with severe disease. The objective of our study was to identify hematological and hemostatic changes associated with severe COVID-19. A total of 170 hospitalized patients with COVID19 were included in the study, defining their severity according to established criteria. Demographic, clinical, and laboratory (days 1, 3, 7, 15) data were obtained. We performed a statistical analysis, assuming significance with a value of p < 0.05. We analyzed the correlation between severity and biomarkers and established cut-off values for severe patients through ROC curves, estimating Odds Ratio associated with severe disease. Day 1 was observed significant differences between moderate vs severe patients for leukocytes (WBC), Neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and D-dimer, establishing cut-off points for each of them. The markers we found associated to risk of severe disease were WBC (OR=3.2396; p = 0.0003), NLR (OR=5.7084; p < 0.0001), PLR (OR=4.4094; p < 0.0001), Neutrophil (OR=4.1193; p < 0.0001), D-dimer (OR=2.7827; p = 0.0124). The results allow to establish basic laboratory biomarkers associated to severe disease, which could be used as prognostic markers.

SAFETY ASSESSMENT OF AYURVEDIC GOLD MEDICATION SUVARNA BHASMA, IN WISTAR RATS

Suvarna bhasma (SB) contains gold and lead, which are highly potent metals and are toxic in their crude form. This study evaluated the safety profile of the medication. Suvarna bhasma was administered for 90 consecutive days orally at three doses (10.23 mg kg-1, 40.92 mg kg-1 and 102.3 mg kg-1 body weight) to wistar rats. Clinical observations, feed and water consumption, and body weight changes were noted during the study period. On the 91st day, hematological, biochemical and histopathological changes were studied. There was a significant decrease in body weight of rats at a low dose (10.23 mg kg-1) at fourth week, which could not be correlated with feed and water consumption findings. Thus, change was not considered as a treatment-related effect. Suvarna bhasma showed no evident untoward effect in haematological, biochemical and histopathological parameters in the 90 day-subchronic toxicity study (OECD 408 guidelines) and was safe in Wistar rats.

Measuring Stress in Animals By Noninvasive Methods

Stress is a biological response in the form of physiological, biochemical, hematological, and behavioral changes to internal or external stimuli that threaten the homeostasis of living beings. Effects that activate the defense system in living things are defined as stressors, and it is possible to talk about many different stress factors. Factors that cause stress can be divided into environmental, physical, social or emotional. Stress reactions, which begin with the effect of the stressor, vary according to the duration and severity of exposure to stress. In animal welfare, stress has many negative effects on organism. These negative effects may cause many problems and, shape future by adding problems such as stress and chain links in animals. Just as well-being is for humans, well-being is very important for animals. To determination of glucocorticoids or metabolites in the bloodstream of an organism under stress, noninvasive methods that provide reliable stress measurement without interfering with the organism have recently become increasingly popular. In this review article study, nineteen articles from various parts of the world were examined. In this review, measures of stress by non-invasive methods by looking at GlucoCorticoid Metabolites (GCM) and the latest developments in this field are discussed. In this review article study, nineteen articles from various parts of the world were examined. As a result of the articles reviewed, non-invasive methods for measuring stress may aid and improve our understanding of stress biology and animal welfare. Applying this method to many animal species and biological materials will provide accurate results and support animal welfare.

Unraveling the Complexity of Vaso-Occlusive Crises in Sickle Cell Disease: Insights from a Resource-Limited Setting.

Background/Objectives: This study investigated vaso-occlusive crises (VOCs) in sickle cell disease in Lubumbashi, Democratic Republic of Congo, aiming to understand the disease complexities amidst limited resources. With sickle cell hemoglobinopathies on the rise in sub-Saharan Africa, this nine-year study explored factors associated with VOCs and hematological components. Methods: This study comprised 838 patients, analyzing VOCs and hematological changes over time. Demographic characteristics and blood composition changes were carefully categorized. A total of 2910 crises were observed and managed, with analyses conducted on severity, localization, and age groups using statistical methods. Results: The majority of crises were mild or moderate, primarily affecting osteoarticular regions. Statistical analysis revealed significant disparities in crisis intensity based on location and age. The association between blood samples and the number of comorbidities was investigated. Significant positive associations were found for all parameters, except monocytes, indicating a potential link between blood variables and complication burden. Survival analysis using Cox regression was performed to predict the probability of experiencing a second crisis. No significant effects of medication or localization were observed. However, intensity (p < 0.001), age (p < 0.001), and gender (p < 0.001) showed significant effects. Adjusted Hazard Ratios indicated increased risk with age and male gender and reduced risk with mild or severe crisis intensity compared to light. Conclusions: This research sheds light on the complexities of VOCs in resource-limited settings where sickle cell disease is prevalent. The intricate interplay between clinical, laboratory, and treatment factors is highlighted, offering insights for improved patient care. It aims to raise awareness of patient challenges and provide valuable information for targeted interventions to alleviate their burden.

Effect of Enteric Fever on Hematological Cells Parameters

Background: Salmonella typhoid and paratyphoid are the agents that cause Enteric fever (Typhoid fever). It is primarily a disease of developing countries because of poor sanitation standards. Fever is a prominent and well-known feature of the illness that increases steadily to a maximum degree. The hematological criteria of an affected individual are changed by this infectious disease. To ascertain the different hematological cells parameters in our sample population, we conducted the investigation. Patients and Methodology: The study, which involved 100 Afghans, was carried out at the Nangarhar University Teaching Hospital between June and November of 2022. A case-control study design was used. Fifty individuals (Cases) who had a confirmed diagnosis of Enteric fever (Typhoid fever) based on typhoid (IgG and IgM) were included in the study. As a control group, fifty healthy individuals with negative typhoid (IgG and IgM) results and no other complaints were included in the study. For both the case and control groups, a complete blood count was performed. Hemoglobin (Hb), White Blood Cell Count (WBC), and Platelet Count (PLT) are regarded as hematological markers. Every patient was also interviewed, and a questionnaire was employed. Numerous demographic factors were questioned, including sex, age, economic status, level of education, and clinical symptoms. SPSS version 16 was then used to analyze the collected data. Results. In the current research, over all 100 patients were enrolled (50 were case, and 50 were control group). The mean age± SD of the patients under the study were 38.74±15.22 years. The most common hematological changes observed were; anemia (32%), thrombocytopenia (24%), leukocytosis (8%) &amp; leucopenia (22%). Fever was the most common presenting symptom (78%) and abdominal symptoms were about 38%. Most of the people were of lower socioeconomic state and were uneducated. Conclusion: This study shows that Enteric fever is bacterial infection and are acquired by enteral route. This disease commonly affects people of low socioeconomical states and those that are uneducated. Fever was the most commonly presenting symptom in these patients. Enteric fever causes changes in the hematological cells parameters which is probably due to bone marrow suppression, which is transient and shows response to therapy.

Mitigating Oxandrolone-Induced Cardiac and Hematological Changes with Bee Venom: Insights from Adult Female Rat Studies

Mitigating Oxandrolone-Induced Cardiac and Hematological Changes with Bee Venom: Insights from Adult Female Rat Studies

Black soldier fly larvae meal in an extruded food: effects on nutritional quality and health parameters in healthy adult cats

Abstract We aimed to evaluate the effects of including black soldier fly larvae meal (BSFL) meal in a dry extruded food on nutritional quality and some health aspects in healthy adult cats. Two dry extruded foods with either poultry meal (control) or 37.5% BSFL meal were fed to eight (3.8-5.2 kg BW; 2.3-y.o.) cats in a cross-over design with two 28-day periods. Food acceptance was recorded throughout the study and samples were collected during the last 7 days of each period for assessment of apparent total tract nutrient digestibility, faecal consistency, fermentation products and microbiota, and blood biochemistry and haematology. Foods were well-accepted and faeces were well-formed with optimal consistency scores. Digestibility values for dry matter, organic matter, nitrogen and gross energy were considered high for the BSFL meal-based food but lower than for the control food (). Unexpectedly, inclusion of BSFL meal had a profound impact on the intestinal microbial activity and composition as illustrated by increased faecal short-chain fatty acids () and biogenic amines concentrations () and reduced bacterial diversity () and shifts in multiple genera (e.g. more Bifidobacterium) in the faeces (). Minor changes in haematology and serum biochemistry parameters were found and deemed not clinically relevant. Overall, this study showed that a BSFL meal-based extruded dry food is readily accepted by healthy adult cats, yields optimal faecal consistency, had suitable nutrient digestibilities and can support their health when fed for 28 days with new leads for impact on feline gut health.

Recirculating aquaculture systems affects hematological parameters and increases ectoparasite susceptibility in Nile tilapia Oreochromis niloticus

We herein evaluated the hematology and parasitology of Nile tilapia previously raised in an earthen pond and transferred to a recirculating aquaculture system (RAS) to verify any possible influence of RAS on such health parameters. For this, 780 tilapias with an initial average weight and length of 91.05 ± 27.08 g and 17.45 ± 1.91 cm, respectively, were distributed in three tanks (2.0 m3) attached to the RAS, where they remained for 54 d. Throughout the period, water quality parameters remained within the range suitable for the cultivation of the species. Tilapia growth performance was satisfactory in the RAS, reaching a final biomass of close to 300 kg and survival of 98%. Hematological and parasitological analyses at the end of 54 d showed possible stress in RAS, with a significant increase in neutrophils from 12.15 ± 6.66 (earthen pond) to 21.43 ± 11.68% (RAS) and erythrocytes from 1.81 ± 0.24 (earthen pond) to 2.13 ± 0.14 (RAS), and a significant decrease in lymphocytes from 22.4 ± 2.66 (earthen pond) to 13.67 ± 3.38 (RAS). Furthermore, parasitological analysis showed a significant increase in the number of parasitized fish (25% to 63%) and abundance (3.55 ± 6.44 to 9.37 ± 9.99) after 54 d. It was concluded that tilapia cultivation in RAS can cause hematological changes and increase parasitism.

Evaluation of Menopausal Syndrome Relief and Anti-Obesity Efficacy of the Korean Fermented Food Doenjang: A Randomized, Double-Blind Clinical Trial.

Foods that help improve menopausal syndrome are being studied worldwide. Doenjang is a traditional Korean fermented soybean food with potential health benefits for menopausal women. In this clinical trial using Doenjang, we aimed to compare the effectiveness of traditional Doenjang and commercial Doenjang in menopausal women. Furthermore, we compared whether Doenjang has a better effect if the number of beneficial microbes is higher. The analyses included the following groups: traditional Doenjang containing either a high dose (HDC; n = 18) or low dose (LDC; n = 18) of beneficial microbes and commercial Doenjang (CD; n = 20). The Kupperman index and hematological changes were examined before and after the use of Doenjang pills. The effects of Doenjang on obesity and body composition were studied before and after ingestion. Lastly, the microorganisms and short-chain fatty acid changes in the stool were compared. The Kupperman index decreased after Doenjang consumption in all three groups, with the greatest decrease in the LDC group. Only the groups that took traditional Doenjang pills exhibited reduced LDL cholesterol. No changes in obesity and inflammation-related indicators were observed. The number of Firmicutes, associated with obesity, decreased in the CD group but the numbers of Bacteroidetes increased in the HDC and CD groups. Thus, traditional Doenjang is more effective in alleviating menopausal syndrome than commercial Doenjang. Further research on the anti-obesity effect or changes in microbiomes and short-chain fatty acids in feces is needed.

Artemisia herba-alba: antioxidant capacity and efficacy in preventing chronic arthritis in vivo.

Arthritis is a debilitating condition impacting the quality of life for millions worldwide, characterized by pain and inflammation. Understanding the mechanisms of arthritis and developing effective treatments are crucial. This study investigated the hydroethanolic extract of Artemisia herba-alba for its protective potential against arthritis hallmarks, oxidative stress, and lipid peroxidation in vitro. It also assessed its in vivo anti-arthritic activity. The phytochemical analysis identified various compounds within the extract, with high concentrations of polyphenols and flavonoids. These compounds are associated with numerous health benefits, making A. herba-alba a potential source of valuable phytochemicals. A. herba-alba demonstrated a notable effect in body weight loss, paw edema, and arthritic severity. Histopathological examination revealed structural improvements in bone and muscle tissues, emphasizing its therapeutic potential in managing chronic arthritis. Furthermore, while these findings are promising, further studies are necessary to delve deeper into the mechanisms underlying the observed hematological changes and to gain a more comprehensive understanding of the in vivo results. This research sets the stage for continued exploration, ultimately aiming to unlock the full potential of A. herba-alba in addressing chronic arthritis and enhancing the lives of those affected by this condition.

Altered Haematological Parameters in Gasoline Station Workers Due to Benzene Exposure

Benzene is harmful to human health and early detection of haematological alterations is important in preventing adverse health effects. This study aimed to investigate the biomarkers of benzene exposure and its effects due to haematological alterations. Gasoline station workers with potential risks according to the biomatrix concerning benzene exposure underwent blood and urine evaluation for the biological monitoring of urinary trans, trans-muconic acid (tt-MA), and haematological and biochemical parameter evaluation. The results were analysed for correlations between biological and haematological effects. The tt-MA biomarker was detected in some workers and approximately 50% of workers had a blood profile that showed abnormal parameters with respect to the haemoglobin (Hb), haematocrit (Hct) and white blood cell parameters, which were outside the normal range. A significant correlation was observed between the tt-MA biomarker’s level and the levels of the haematological and biochemical parameters, which were Hb, Hct, eosinophil, neutrophil, SGOT and blood creatinine. The level of urinary tt-MA as a marker of benzene exposure correlated with haematological and biochemical changes in the blood, suggesting that the gasoline station workers were affected by benzene exposure. Moreover, the current study suggests that early detection of haematological abnormalities may be possible by analysing biomarkers of their effects through regular health surveillance of workers.

Survivors of polymicrobial sepsis are refractory to G-CSF-induced emergency myelopoiesis and hematopoietic stem and progenitor cell mobilization

Sepsis survivors exhibit immune dysfunction, hematological changes, and increased risk of infection. The long-term impacts of sepsis on hematopoiesis were analyzed using a surgical model of murine sepsis, resulting in 50% survival. During acute disease, phenotypic hematopoietic stem and progenitor cells (HSPCs) were reduced in the bone marrow (BM), concomitant with increased myeloid colony-forming units and extramedullary hematopoiesis. Upon recovery, BM HSPCs were increased and exhibited normal function in the context of transplantation. To evaluate hematopoietic responses in sepsis survivors, we treated recovered sham and cecal ligation and puncture mice with a mobilizing regimen of granulocyte colony-stimulating factor (G-CSF) at day 20 post-surgery. Sepsis survivors failed to undergo emergency myelopoiesis and HSPC mobilization in response to G-CSF administration. G-CSF is produced in response to acute infection and injury to expedite the production of innate immune cells; therefore, our findings contribute to a new understanding of how sepsis predisposes to subsequent infection.

Dependence of hematological parameters on the microbiota of the nasal mucosa in chronic rhinitis

Relevance. Chronic rhinitis is one of the most common otorhinolaryngological diseases. We assume that dysbiosis of the nasal mucosa depends on the phenotype of chronic rhinitis and affects hematological characteristics.&#x0D; Aim. Study of the hematological parameters dependence from the microbiota of the nasal mucosa in patients with various forms of chronic rhinitis.&#x0D; Material and methods. The examination groups included 45 patients with chronic allergic rhinitis (CAlR), 49 patients with chronic vasomotor rhinitis (CVR), 32 patients with chronic atrophic rhinitis (CAR), 39 patients with chronic infectious rhinitis (CIR) and 40 people from the control group. The subjects with chronic rhinitis, as well as the control group, underwent bacteriological examination of the nasal mucosa and assessment of hematological parameters. Statistical analysis was carried out using the Statistica 10 package.&#x0D; Results. The microbiome of the nasal mucosa is characterized by 100% infection with infectious agents in CIR, while fungal flora was detected in 1/3 of cases. The microbiological features of the nasal mucosa in CVR corresponded to the control, in CAR rhinitis - predominantly the growth of Klebsiella spp., in CIR - Staphylococcus spp., Streptococcus spp., Neisseriae spp., Escherichia spp. Hematological features manifested themselves depending on the form of chronic rhinitis. The greatest number of changes in blood parameters is found in CIR and CAR, less in CAlR, no changes were detected in CVR. The greatest dysbiosis of the nasal mucosa in CIR, the abundant growth of microorganisms of the genus Klebsiella spp in CAR corresponds to the largest number of changes in hematological parameters, and CVR is characterized by the absence of changes in hematological parameters, despite the fact that the microbiological characteristics of the nasal mucosa corresponded to the control.&#x0D; Conclusion. The microbiota of the nasal mucosa in healthy people and patients from the groups with CAlR, CVR and CAR is represented by a combination of coccal and rod-shaped flora, with the exception of CIR, in which representatives of the fungal flora are identified. Pathogenetically significant is the identification of representatives of Staphylococcus spp., Streptococcus spp., Neisseriae spp., Escherichia spp. in CIR, and Klebsiella spp. in CAR. Most hematological changes are detected in chronic atrophic and infectious rhinitis.

Detection of haptoglobin and serum amyloid A as biomarkers in naturally infected Mycoplasma bovis calves

The livestock sector of Pakistan is increasing rapidly and it plays important role both for rural community and national economy. It is estimated that almost 8 million rural people are involved in livestock rearing and earning about 35–40 % of their income from the livestock sector. Mycoplasma bovis (M. bovis) infection causes significant economic losses in dairy animals especially young calf in the form of clinical illnesses such as pneumonia, poly-arthritis, respiratory distress and mortality. M. bovis is hard to diagnose and control because of uneven disease appearance and it is usually noticed in asymptomatic animals. For the identification of M. bovis in sub-clinical and clinical samples, determination of acute phase proteins i.e., haptoglobin (Hp) and serum amyloid A (SAA) are important tools for the timely diagnosis of disease. Therefore, early diagnosis of disease and hemato-biochemical changes are considered beneficial tools to control the infectious agent to uplift the economy of the dairy farmers. For this purpose, blood samples were collected from 200 calves of Bovidae family. Serum was separated from blood samples to determine the concentration of Hp and SAA, while blood samples were processed to determine hematological changes in blood from calves by using hematological analyzer. The blood plasma obtained from the blood samples was processed to measure oxidative stress factors. Lungs tissues from slaughterhouses/ morbid calves were collected to observe histopathological changes. The results of present study indicated that level of SAA and Hp remarkably increased (P < 0.05) in M. bovis infected calves in comparison to healthy calves. The oxidative stress markers indicated that nitric oxide and MDA levels in the infected calves increased significantly (P < 0.05), while infected claves had considerably lower levels of superoxide dismutase, catalase and glutathione. These findings indicate that oxidative stress play role to increase the level of APPs, while monitoring of APPs levels may serve as a valuable addition to the clinical evaluation of naturally infected calves with M. bovis. The hematological parameters were decreased significantly (P < 0.05). Altogether, this study suggests that Hp and SAA are proposed as promising biomarkers for detecting naturally occurring M. bovis infection in calves.