Changes In δ13C Research Articles

Tracing service crops' net carbon and nitrogen rhizodeposition into soil organic matter fractions using dual isotopic brush-labeling

Recent studies emphasize the importance of rhizodeposition in soil organic matter (SOM) formation. However, its quantification in different soil fractions is still uncommon. To estimate the net carbon (C) and nitrogen (N) rhizodeposition into particulate (POM) and mineral-associated (MAOM) organic matter, we conducted a pot experiment to trace plant-derived C and N into the soil. In this study, we first evaluated the effectiveness of the dual isotope (13C and 15N) brush-labeling method on oat and vetch plants, two species commonly sown as service crops, to investigate in situ plant-soil interactions. Our results indicate that foliar brushing successfully labeled plant tissues and traced plant-derived C and N into the soil, allowing us to make a conservative estimate of net rhizodeposition. Based on soil δ13C and δ15N changes, we show that most of the net C (81.5 and 100% for oats and vetch, respectively) and N (92.4 and 93.2% for oats and vetch, respectively) rhizodeposition contributes directly to the formation of MAOM and not POM. Both species contributed similar amounts of net C rhizodeposition to the MAOM fraction (about 0.59 g plant−1), while oat net C rhizodeposition also formed a small amount of POM (0.138 g plant−1). Because vetch had low root biomass, the net C rhizodeposition:root C ratio was much higher in vetch than in oats (12.3 and 0.65, respectively). For net N rhizodeposition, vetch contributions were greater than oats in both fractions (71.4% greater in MAOM and 50.4% greater in POM). Our estimates of net C and N rhizodeposition range from 15 to 40% of the total recovered C or N. Our results demonstrate the importance of incorporating rhizodeposition into belowground biomass estimates and SOM models, and suggest that service crops provide large amounts of C and N inputs from living roots that are mainly retained in MAOM.

A first high-resolution carbon isotope stratigraphy from the Boreal (NW Germany) for the Berriasian to Coniacian interval—implications for the timing of the Aptian–Albian boundary

High-amplitude changes in sedimentary δ13C characterize the Cretaceous system and have been proven useful for supra-regional chemostratigraphic correlation. In the Cretaceous, these δ13C perturbations indicate large shifts between the global carbon reservoirs that are usually caused by volcanic activity of large igneous provinces, the widespread deposition of thick organic carbon-rich sequences and/or changes in orbital parameters. Here, we present an upper Berriasian to lower Coniacian (c. 142–88 Ma) composite carbon isotope record based on 14 drill cores, 2 outcrops, and almost 5,000 samples. The total record comprises a composite thickness of more than 1,500 m. All cores and successions are located in the larger Hanover area, which represents the depocenter of the North German Lower Saxony Basin in Early to mid-Cretaceous times. In Northern Germany, Boreal Lower Cretaceous sediments are predominantly represented by CaCO3-poor mud and siltstones of up to 2,000 m thickness, which become more carbonate-rich during the Albian–Cenomanian transition and even chalkier in the upper Cenomanian to Coniacian interval. The carbon isotope record reveals a number of global key events, including the Valanginian Weissert Event, the Oceanic Anoxic Events (OAEs) 1a and d, and the Kilian Event (Aptian–Albian boundary, part of OAE 1b). For the early Late Cretaceous, the Mid-Cenomanian Event, the OAE 2 (Cenomanian–Turonian Boundary Event), and the Navigation Event, among others, have been identified. The Kilian Event represents the Aptian–Albian boundary and has been identified herein for the first time in Northern Europe. Based on the evaluation of its relative position to the Vöhrum boundary tuff, we tentatively propose a slightly older age for the Aptian–Albian boundary of c. 113.65 Ma instead of 113.2 Ma. The observed chemostratigraphic events enable a detailed stratigraphic comparison with Tethyan and other Boreal records and associated paleoenvironmental data. Thus, this new detailed chemostratigraphy provides a unique opportunity to potentially overcome many still existing Boreal–Tethyan correlation issues. The presented record can be considered almost complete, albeit a 2-Myr gap during the early Albian is likely, and condensed intervals occur specifically during the lower Aptian.

Raman Spectroscopy for Urea Breath Test.

The urea breath test is a non-invasive diagnostic method for Helicobacter pylori infections, which relies on the change in the proportion of 13CO2 in exhaled air. Nondispersive infrared sensors are commonly used for the urea breath test in laboratory equipment, but Raman spectroscopy demonstrated potential for more accurate measurements. The accuracy of the Helicobacter pylori detection via the urea breath test using 13CO2 as a biomarker is affected by measurement errors, including equipment error and δ13C measurement uncertainty. We present a Raman scattering-based gas analyzer capable of δ13C measurements in exhaled air. The technical details of the various measurement conditions have been discussed. Standard gas samples were measured. 12CO2 and 13CO2 calibration coefficients were determined. The Raman spectrum of the exhaled air was measured and the δ13C change (in the process of the urea breath test) was calculated. The total error measured was 6% and does not exceed the limit of 10% that was analytically calculated.

Atlantic circulation changes across a stadial–interstadial transition

Abstract. We combine consistently dated benthic carbon isotopic records distributed over the entire Atlantic Ocean with numerical simulations performed by a glacial configuration of the Norwegian Earth System Model with active ocean biogeochemistry in order to interpret the observed Cibicides δ13C changes at the stadial–interstadial transition corresponding to the end of Heinrich Stadial 4 (HS4) in terms of ocean circulation and remineralization changes. We show that the marked increase in Cibicides δ13C observed at the end of HS4 between ∼2000 and 4200 m in the Atlantic can be explained by changes in nutrient concentrations as simulated by the model in response to the halting of freshwater input in the high-latitude glacial North Atlantic. Our model results show that this Cibicides δ13C signal is associated with changes in the ratio of southern-sourced (SSW) versus northern-sourced (NSW) water masses at the core sites, whereby SSW is replaced by NSW as a consequence of the resumption of deep-water formation in the northern North Atlantic and Nordic Seas after the freshwater input is halted. Our results further suggest that the contribution of ocean circulation changes to this signal increases from ∼40 % at 2000 m to ∼80 % at 4000 m. Below ∼4200 m, the model shows little ocean circulation change but an increase in remineralization across the transition marking the end of HS4. The simulated lower remineralization during stadials compared to during interstadials is particularly pronounced in deep subantarctic sites, in agreement with the decrease in the export production of carbon to the deep Southern Ocean during stadials found in previous studies.

Round goby (Neogobius melanostomus) δ13C/δ15N discrimination values and comparisons of diets from gut content and stable isotopes in Oneida Lake.

Gut content analyses have found that round gobies (Neogobius melanostomus) are highly dependent on dreissenid mussels but stable isotope analysis has often suggested that the dreissenid contribution is lower. However, estimation of dietary contributions with stable isotopes relies on accurate discrimination factors (fractionation factors). To test if discrimination values commonly used in aquatic food web studies are suitable for round gobies, we collected round gobies from Oneida Lake, raised them for 63 days under four different diets (Chironomus plumosus, Mytilus chilensis, Dreissenia spp., Euphausia superba) and measured the change in white muscle δ13C and δ15N. Gobies were also collected throughout Oneida Lake for gut content and stable isotope analysis. Diets changed as round gobies grew, with small round gobies (17-42mm) feeding mostly on cladocera and chironomids, intermediate sized gobies (43-94mm) transitioning from chironomid to dreissenid consumption, and larger gobies (95-120mm) predominantly consuming dreissenids, similar to findings in other studies. Discrimination factors were obtained by fitting a commonly used asymptotic regression equation describing changes in fish δ13C and δ15N as a function of time and diet stable isotope ratios. The discrimination factor determined for δ13C (-0.4‰ ± 0.32, SE) was lower than the "standard" value of 0.4‰, while that of δ15N (4.0‰ ± 0.32, SE) was higher than the standard value of 3.4‰. Turnover rates for both δ13C and δ15N were estimated as 0.02 ‰*day-1. The use of experimentally determined discrimination factors rather than "standard" values resulted in model estimates that agree more closely with the observed increasing importance of dreissenids in gut content of larger gobies. Our results suggest that the importance of dreissenid mussels inferred from stable isotope studies may be underestimated when using standard isotopic discrimination values.

Determination of Paleoenvironmental Changes by Using δ13C, 14C Dating and Rb/Sr Ratio in Critical Karst Area of Yunnan-Guizhou Plateau, Southwestern China

Paleosols preserve archives of vegetation history, environmental changes, and sedimentary systems. The changes in vegetation history and environmental conditions in karst areas of the Yunnan–Guizhou plateau of southwestern China since the late Pleistocene were analyzed using δ13C, 14C and the Rb/Sr ratio. Our δ13C results reveal the coexistence of C3 and C4 plants (−10.91 to −30.45‰) in soil organic matter, something that is consistent with the present field vegetation assessment. The large vertical difference in the δ13C value (>4‰) is related to the cultivation of agricultural crops and to changes in environmental conditions. Furthermore, the variation in δ13C values was influenced by the summer monsoon climate of the Indian Ocean and east Asia. We found that total organic carbon (TOC) concentration decreased exponentially with soil depth, indicating high carbon loss. In addition, 14C apparent age of the soil profiles ranged from modern times to 20,605 (±63) years before the present (yrs. BP) and is positively correlated with soil depth. The combined results indicate that, since the late Pleistocene era, environmental conditions in the study area have changed intermittently on a scale of millennia from hot-humid to cold-dry conditions. We speculate that there may have been extreme climate events in the study area 14,750 years BP, which are marked by a drastic change in δ13C and in the Rb/Sr ratio.

Radio- and stable carbon isotope analysis reveals minimal assimilation of petrogenic carbon into an oligotrophic freshwater food web after experimental spills of diluted bitumen

Following an oil spill into water, bacteria can biodegrade petroleum hydrocarbons which could lead to petrogenic carbon assimilation by aquatic biota. We used changes in the isotope ratios of radio- (Δ14C) and stable (δ13C) carbon to examine the potential for assimilation of petrogenic carbon into a freshwater food web following experimental spills of diluted bitumen (dilbit) into a boreal lake in northwestern Ontario, Canada. Different volumes (1.5, 2.9, 5.5, 18, 42, 82, and 180 L) of Cold Lake Winter Blend (a heavy crude blend of bitumen and condensate) dilbit were applied to seven 10-m diameter littoral limnocorrals (approximate volume of 100 m3), and two additional limnocorrals had no added dilbit to serve as controls. Particulate organic matter (POM) and periphyton from oil-treated limnocorrals had lower δ13C (up to 3.2‰ and 2.1‰ for POM and periphyton, respectively) than the control at every sampled interval (3, 6 and 10 weeks for POM and 6, 8 and 10 weeks for periphyton). Dissolved organic and inorganic carbon (DOC and DIC, respectively) had lower Δ14C in the oil-treated limnocorrals relative to the control (up to 122‰ and 440‰ lower, respectively). Giant floater mussel (Pyganodon grandis) housed for 25 days in aquaria containing oil-contaminated water from the limnocorrals did not show significant changes in δ13C values of muscle tissue compared to mussels housed in control water. Overall, the changes in δ13C and Δ14C observed indicated small amounts (up to 11% in DIC) of oil carbon incorporation into the food web. The combined δ13C and Δ14C data provide evidence for minimal incorporation of dilbit into the food web of this oligotrophic lake, suggesting that microbial degradation and subsequent incorporation of oil C into the food web may play a relatively small role in the ultimate fate of oil in this type of ecosystem.

Littoral enchytraeids and Eisenia fetida earthworms facilitate utilization of marine macroalgae as biofertilizers

Marine macroalgae are often used as biofertilizers in the coastal regions across the world. However, the effects of soil fauna in macroalgae decomposition are not fully understood. We conducted a microcosm experiment to assess the possibility of using a mixture of native Enchytraeidae and the model earthworm species Eisenia fetida to accelerate decomposition of dominant algae taxa from White Sea (Fucus spp.) and Black Sea (Cystoseira spp.) after their amendment to soil with simultaneous mitigation of the associated carbon dioxide (CO2) emissions. During 45 days of the microcosm experiment, we measured CO2 emission from the soil and evaluated changes in macroalgal biomass, δ13C and δ15N isotopic signatures of soil, macroalgae, earthworm faeces and enchytraeid tissues as well as bulk carbon and nitrogen content in soil. The mass of Cystoseira spp. residues, unlike that of Fucus spp., significantly decreased during the experiment (by approximately 73 % of the initial level). This effect was amplified by the co-action of E. fetida and Enchytraeidae (by 65 % of the initial). The addition of Enchytraeidae into microcosms led to the more than two-fold reduction of CO2 emission in all treatments. Differential enrichment of soil and earthworm casts with macroalgae-derived carbon and nitrogen, as suggested by stable isotope analysis, indicated that macroalgae-originating carbon pathway is rather driven by a synergistic activity of enchytraeids and earthworms (δ13C enrichment up to 4.8 ‰). Nitrogen transfer was dependent on the microbial activity and could be modulated and channelized by enchytraeids, whose δ15N values strictly corresponded to algal taxa. This highlights the importance of annelids in increasing the value of macroalgae as biofertilizers.

Orbital‐Insolation Controlled Porites Coral δ13C Seasonality Variations Since the Mid‐Holocene in the Northern South China Sea

AbstractCoral skeleton δ13C is a routinely measured indicator in modern insolation change research, but the knowledge of environmental and climatic signals recorded in its seasonality during geological time is sparse. In this study, we present eight Porites coral δ13C records from the mid‐late Holocene to the present from the northern South China Sea (NSCS). Compared with the main control factors for modern δ13C changes, coral δ13C seasonality in the NSCS since the mid‐Holocene shows a long‐term decreasing trend, which is consistent with the change trend as orbital‐induced (precession) insolation seasonal amplitude. By excluding other influencing factors, we speculate that the stronger coral δ13C seasonality (18.8%) over the mid‐Holocene than modern period is attributable to the metabolic effect, which predicts the stronger coral δ13C seasonality under stronger insolation seasonality. Our study has implications for coral δ13C seasonality as a potential indicator to record past insolation information under different climatic backgrounds.

Stable C and N isotope variation during anaerobic digestate composting and in the compost-amended soil-plant system

Although the use of composts derived from anaerobic digestates as soil amendments is likely to increase in the future, there is little information concerning the fate of their C and N compounds after their incorporation into soil. This work assesses C and N concentrations and the associated changes in δ15N and δ13C during the composting processes of cattle and pig slurry anaerobic digestates. In addition, the compost effect on C and N fractions and plant uptake were studied during a six-month pot experiment with rosemary plants. The results did not show δ13C and δ15N isotopic discrimination during composting, indicating a previous stabilization of cattle manure and pig slurry during the anaerobic digestion. This fact was also confirmed by the low C losses during the composting processes (1.2-fold and 1.05-fold for the composting piles with cattle and pig slurry anaerobic digestates, respectively). After soil addition, the composts augmented N values (from 0.41 g kg−1 to around 0.56 g kg−1 in low dose and 0.68 g kg−1 in high dose compost amended soils) and δ15N soil values (increases in the range of 50%–156%), but showed only slight differences in C and δ13C values compared to unfertilised control and inorganic fertilized soils. Moreover, the rosemary leaves of the plants grown on the compost amended soils presented higher N and δ15N abundance than control and inorganic fertilized plants. We conclude that δ15N abundance of anaerobic digestate composts is useful to discern its N uptake and could thus be a useful tool to detect whether organic or mineral fertiliser types were used for agricultural production.

Changes in tree leaf δ13C along climatic and geographical gradients in China

Changes in tree leaf δ13C along climatic and geographical gradients in China

Methane Concentration and δ13C Isotopic Signature in Methane over Arctic Seas in Summer and Autumn 2020

We have studied the assessment of methane content in the near atmospheric layer of the Arctic seas and the sources of its emissions. Continuous atmospheric measurements of methane concentration and δ13C isotope signature in methane were carried out during two expeditions aboard the R/V Akademik Mstislav Keldysh in the summer and autumn of 2020. The minimum CH4 concentration (mean 1.937 ± 0.010 ppm) were obtained over the North and Norwegian Seas. The maximum CH4 concentration took place above the Kara (1.998 ± 0.030 ppm on average, maximum values are up to 2.000–2.040 ppm) and White (2.006 ± 0.030 ppm on average) seas. The average values of δ13C in the studied region were close to that typical for the Northern Hemisphere: –47.5‰. Regional changes in δ13C allowed CH4 sources to be identified and showed that the methane concentration in the surface atmospheric level is mainly affected by the direction of air-mass advection.

Spatio-Temporal Variations in Carbon Isotope Discrimination Predicted by the JULES Land Surface Model.

Stable carbon isotopes in plants can help evaluate and improve the representation of carbon and water cycles in land-surface models, increasing confidence in projections of vegetation response to climate change. Here, we evaluated the predictive skills of the Joint UK Land Environmental Simulator (JULES) to capture spatio-temporal variations in carbon isotope discrimination (Δ13C) reconstructed by tree rings at 12 sites in the United Kingdom over the period 1979-2016. Modeled and measured Δ13C time series were compared at each site and their relationships with local climate investigated. Modeled Δ13C time series were significantly correlated (p<0.05) with tree-ring Δ13C at eight sites, but JULES underestimated mean Δ13C values at all sites, by up to 2.6‰. Differences in mean Δ13C may result from post-photosynthetic isotopic fractionations that were not considered in JULES. Inter-annual variability in Δ13C was also underestimated by JULES at all sites. While modeled Δ13C typically increased over time across the UK, tree-ring Δ13C values increased only at five sites located in the northern regions but decreased at the southern-most sites. Considering all sites together, JULES captured the overall influence of environmental drivers on Δ13C but failed to capture the direction of change in Δ13C caused by air temperature, atmospheric CO2 and vapor pressure deficit at some sites. Results indicate that the representation of carbon-water coupling in JULES could be improved to reproduce both the trend and magnitude of interannual variability in isotopic records, the influence of local climate on Δ13C, and to reduce uncertainties in predicting vegetation-environment interactions.

Shade and drought increase fungal contribution to partially mycoheterotrophic terrestrial orchids Goodyera pubescens and Tipularia discolor

Many photosynthetic plants supplement photosynthetic carbon with fungal carbon, but the mechanisms that govern dependence on mycoheterotrophic carbon are poorly understood. We used exclusion shelters to manipulate water and light availability to plants of the terrestrial orchids Goodyera pubescens and Tipularia discolor. We tracked changes in δ13C from photosynthesis and δ15N acquired from soil-derived inorganic nitrogen versus mycoheterotrophy, along with direct measures of photosynthesis in T. discolor. We hypothesized that shade would increase dependence on mycoheterotrophy compared to reference plants, while drought would decrease both photosynthesis and the abundance of potential mycorrhizal fungi. Drought and shade enriched 13C and 15N in both G. pubescens and T. discolor, compared to control plants, indicating increased fungal contribution to orchid tissues. Physiological measurements of T. discolor leaves showed that dark respiration, water use efficiency, and relative electron transport rate did not vary significantly, but shaded plants had greater quantum efficiency, suggesting they were light-limited. Light saturated photosynthesis of T. discolor leaves was lower in both shaded and drought-treated plants, indicating lower photosynthetic capacity, and likely greater dependence on mycoheterotrophy and corresponding enrichment in 13C and 15N. This study documented changes in orchid dependence on fungal carbon in response to manipulated environmental conditions. Both shade and drought increased the dependence of both orchids on mycoheterotrophically derived carbon and nitrogen.

Insights into atrazine degradation by thermally activated persulfate: Evidence from dual C–H isotope analysis and DFT simulations

SO4– -assisted oxidation was of great concern and the accurate interpretation of reaction pathway was crucial. Innovatively, this study introduced compound-specific isotope fractionation analysis (CSIA) into atrazine (ATZ) degradation by heat-activated persulfate (PS), in order to provide “in-situ” and “fingerprinted” analysis of reaction contribution and bond cleavage process based on isotope fractionation. As results, radical oxidants including SO4- and OH were generated through thermal activation of PS, and proved to play dominate roles in ATZ degradation. Inconceivably, the isotope fractionation by single SO4-dominated reaction showed weak and positive C isotope fractionation, with εC of 1.58 ‰ at pH 4 and 0.65 ‰ at pH 7, respectively. Reactions by single SO4- and OH can be distinguished resulting from their significantly different ε and ɅC/H values. Changes of δ13C and δ2H values during ATZ degradation by heat-activated PS followed Rayleigh equation well, with the εC, εH and ɅC/H values of −1.5 ‰, −21.1 ‰, 6.60 at pH 4 and −2.7 ‰, −36.6 ‰, 10.02 at pH 7 respectively. The relative contribution of SO4- radical to ATZ degradation was ca.100 % at pH 4 and 83.9 % at pH 7. The isotope fractionation study and DFT calculations suggested that single electron transfer with the cleavage of C-Cl bond was dominated and displayed inverse C isotope fractionation for SO4- oxidation, which was different from that of OH oxidation and more selective to dehalogenate reaction. This research emphasized the promising technology of CSIA, which can achieve accurate identification of reaction contribution and pathways in advanced oxidation process.

The 8.2 ka event in northern Spain: timing, structure and climatic impact from a multi-proxy speleothem record

Abstract. The 8.2 ka event is regarded as the most prominent climate anomaly of the Holocene and is thought to have been triggered by a meltwater release to the North Atlantic that was of sufficient magnitude to disrupt the Atlantic Meridional Overturning Circulation (AMOC). It is most clearly captured in Greenland ice-core records, where it is reported as a cold and dry anomaly lasting ∼ 160 years, from 8.25 ± 0.05 until 8.09 ± 0.05 ka (Thomas et al., 2007). It is also recorded in several archives in the North Atlantic region; however, its interpreted timing, evolution and impacts vary significantly. This inconsistency is commonly attributed to poorly constrained chronologies and/or inadequately resolved time series. Here we present a high-resolution speleothem record of early Holocene palaeoclimate from El Soplao Cave in northern Spain, a region pertinent to studying the impacts of AMOC perturbations on south-western Europe. We explore the timing and impact of the 8.2 ka event on a decadal scale by coupling speleothem stable carbon and oxygen isotopic ratios, trace element ratios (Mg / Ca and Sr / Ca), and growth rate. Throughout the entire speleothem record, δ18O variability is related to changes in effective recharge. This is supported by the pattern of changes in δ13C, Mg / Ca and growth rate. The 8.2 ka event is marked as a centennial-scale negative excursion in El Soplao δ18O, starting at 8.19 ± 0.06 ka and lasting until 8.05 ± 0.05 ka, suggesting increased recharge at the time. Although this is supported by the other proxies, the amplitude of the changes is minor and largely within the realm of variability over the preceding 1000 years. Further, the shift to lower δ18O leads the other proxies, which we interpret as the imprint of the change in the isotopic composition of the moisture source, associated with the meltwater flux to the North Atlantic. A comparison with other well-dated records from south-western Europe reveals that the timing of the 8.2 ka event was synchronous, with an error-weighted mean age for the onset of 8.23 ± 0.03 and 8.10 ± 0.05 ka for the end of the event. This compares favourably with the North Greenland Ice Core Project (NGRIP) record. The comparison also reveals that the El Soplao δ18O is structurally similar to the other archives in south-western Europe and the NGRIP ice-core record.

Eutrophication has a greater influence on floodplain lake carbon cycling than dam installation across the middle Yangtze region

Carbon cycling in shallow floodplain lakes is complex due to variability in delivery of flood-derived allochthonous organic matter (OM). Human activities have potential to significantly modify the carbon balance of lakes by damming which restricts external OM inputs and via eutrophication which can increase the in-lake production of algae and/or aquatic plants. In order to understand how these human activities influence carbon cycling in shallow floodplain lakes over decadal-centennial timescales, we analysed C/N ratios and δ13C from terrestrial plants, catchment soils, aquatic plants and dated sediment cores from six heavily modified lakes in the middle Yangtze floodplain. Submerged macrophytes (−21.4 ± 4.6 ‰) had higher δ13C than C3 plants from the catchment (−26.6 ± 0.6 ‰) and emergent and floating plants (−26.6 ± 4.0 ‰). Increases in sedimentary chlorophyll a (from primary producers) were associated with a decline in sedimentary δ13C in the severely eutrophic Dongting, Luhu, Wanghu and Poyang Lakes after the 1980s. In contrast, sedimentary δ13C increased in Honghu and Futou Lakes which have abundant submerged macrophytes. The timing and scale of sedimentary δ13C changes indicated stronger responses to eutrophication than damming, with eutrophication responses ranging from a macrophyte proliferation to the dominance of phytoplankton.

δ13C in muscle, liver, and adipose fin and their relationship to weight change during both growth and starvation in rainbow trout (Oncorhynchus mykiss), after feeding a diet low in 13C

A 35-day experiment was conducted with a total of 105 rainbow trout (Oncorhynchus mykiss) with an average initial weight of 192.9 g, sampled over four time points (day 7, 14, 21, and 28) to study the relationship between respectively, δ13C in muscle, liver, and adipose fin and relative weight gain, when fed a diet based on plant C3 ingredients, with a lower level of δ13C compared to the baseline diet. From day 28 to 35, seven fish per tank were starved to investigate the effect of starvation on δ13C in muscle, liver, and the adipose fin. Relative weight gain (as a proxy for feed efficiency) significantly (P < 0.0001) affected δ13C in muscle and liver, respectively, implying that a diet with a lower natural abundance (compared to the baseline diet) can be used to phenotype feed efficiency. Moreover, the change in δ13C due to weight loss was considerable in liver (2.6‰), but only 1.7‰ in muscle under starvation.

Isotopic evidence concerning the habitat of Nautilus macromphalus in New Caledonia.

Modern nautilids (Nautilus and Allonautilus) have often been studied by paleontologists to better understand the anatomy and ecology of fossil relatives. Because direct observations of these animals are difficult, the analysis of light stable isotopes (C, O) preserved in their shells has been employed to reveal their habitat and life history. We aim to (1) reconstruct the habitat depth of Nautilus macromphalus and (2) decipher the fraction of metabolic carbon in its shell by analyzing oxygen and carbon isotopes (δ18O, δ13C) in the septa of two specimens in combination with analyses of water samples from the area. Additionally, we investigate whether morphological changes during ontogeny are reflected in the isotopic values of the shells. Results reveal that the patterns of change of δ18O and δ13C in the septa of N. macromphalus pre- and post-hatching are consistent with previous studies. Values of δ18Owater range from 0.7 to 1.4‰ (VSMOW), with a maximum value coincident with a salinity maximum at ~150 m. We use the temperature and δ18Owater profiles to calculate equilibrium values of δ18Oaragonite with depth. Comparing these values with the measured δ18O of the septa shows that the habitat depth of N. macromphalus is ~140 m pre-hatching and ~370 m post-hatching. Using δ13C of shell carbonate and published data on metabolic carbon, the fraction of metabolic carbon is reconstructed as ~21% and 14% pre- and post-hatching, respectively. The reconstructed depth pre-hatching is slightly shallower than in N. pompilius from the Philippines and Fiji, but the post-hatching depth is similar. However, it is important to emphasize that these estimates represent average over time and space because nautilus is a mobile animal. Lastly, the changes in morphological parameters and the changes in δ13C and δ18O during ontogeny do not coincide except at hatching and at the onset of maturity.

Natural alleles of the abscisic acid catabolism gene ZmAbh4 modulate water use efficiency and carbon isotope discrimination in maize.

Altering plant water use efficiency (WUE) is a promising approach for achieving sustainable crop production in changing climate scenarios. Here, we show that WUE can be tuned by alleles of a single gene discovered in elite maize (Zea mays) breeding material. Genetic dissection of a genomic region affecting WUE led to the identification of the gene ZmAbh4 as causative for the effect. CRISPR/Cas9-mediated ZmAbh4 inactivation increased WUE without growth reductions in well-watered conditions. ZmAbh4 encodes an enzyme that hydroxylates the phytohormone abscisic acid (ABA) and initiates its catabolism. Stomatal conductance is regulated by ABA and emerged as a major link between variation in WUE and discrimination against the heavy carbon isotope (Δ13C) during photosynthesis in the C4 crop maize. Changes in Δ13C persisted in kernel material, which offers an easy-to-screen proxy for WUE. Our results establish a direct physiological and genetic link between WUE and Δ13C through a single gene with potential applications in maize breeding.