Changes In Content Research Articles

Temperature-copper content induced wear mechanism transition in Cu/Ni60A composites

Coppery blast furnace tuyere is easily damaged in high temperature wear environment. Preparing a Ni60A coating by plasma cladding is an effective method to improve its wear resistance and service life. Controlling the appropriate dilution rate is the key to obtain high-quality coatings. The existing research has no clear conclusion on the range of dilution rate of coating for coppery tuyere. In this paper, a series of Cu/Ni60A composites with different copper content (10 wt%, 20 wt%, 40 wt% and 60 wt%) were prepared to mimic Ni60A coatings exhibiting distinct dilution rate. Under the coupling change of temperature and copper content, the wear mechanism of Cu/Ni60A composites showed a complex transition law. Copper content, surface oxide layer (NiO or CuO) and high-temperature softening temperature were the decisive factors of wear transition. The applicable temperatures of Cu/Ni60A composites with low copper content (10 %, 20 %), middle copper content (40 %) and high copper content (60 %) were about 600 °C, 400 °C and 300 °C, respectively. Combined with the heat transfer analysis results, the suitable dilution rates for the coating applied to the inner wall (＜10 %) and front end (10 %-20 %) of tuyere was revealed.

Research on the capillary absorption capacity of concrete of different particle sizes after high temperature based on nuclear magnetic resonance technology

Concrete is subjected to thermal stress and thermal loading in a high temperature environment, resulting in the expansion and contraction of internal pores, which adversely affects the stability and durability of concrete structures. To explore the effects of temperature and aggregate particle size on the capillary absorption capacity of concrete, the capillary absorption tests were carried out on the specimens with aggregate particle sizes of 1.25–2.5 mm, 2.5–5.0 mm and 5.0–10 mm after high temperature treatment at 300 °C, 400 °C and 500 °C.In this paper, The capillary absorption coefficient was measured by mass change method, and the migration change law and spatial distribution of water during capillary absorption were monitored by NMR technology, the changes in water content in different pores during water absorption were calculated by nuclear magnetic resonance T2 spectroscopy, and a method for calculating the capillary absorption coefficient based on T2 spectra was proposed. The results showed that the capillary absorption coefficient of the samples without high-temperature treatment decreased with increasing of particle size. The capillary absorption coefficient increases at 300 °C and decreases slightly at 400 °C. At 500 °C, the capillary absorption coefficient increases dramatically. This shows that at 500 °C, the durability of concrete is drastically affected. The capillary absorption coefficient calculated based on the T2 spectrum is linearly related to the results of the traditional weighing method. In this paper, the fractal geometry theory and regarding methods are used to deduce the relationship between the average capillary rise height and t12DT, and the rise rate is related to the pore structure. And the correctness of the model was verified by MRI. This study can provide theoretical guidance for the safety assessment of concrete structures after high temperature exposure.

Effect of partial substitution of NaCl by KCl on aggregation behavior and gel properties of beef myosin

Based on the three typical gels under KCl substitution groups, the effect of partial substitution of NaCl by KCl (groups: T 1:0.6 M NaCl; T 2: 0.3 M NaCl +0.3 M KCl; T 3: 0.2 M NaCl +0.4 M KCl; T 4:0.6 M KCl) on the aggregation behavior and gel characteristics of myosin was evaluated. The significant changes in hydrophobicity and sulfhydryl content (P < 0.05) indicate KCl substitution enhances myosin aggregation through hydrophobic interactions and disulfide bonds. According to Ca2+-ATP, scanning electron microscopes (SEM) and the rheological results, T2 had a smoother network structure at about 75 °C. Noticeably, T3 had high water holding capacity (WHC), but its gel had some visible cavities. T4 had a gel structure with several irregular aggregates due to a greater aggregation rate. Thus, appropriate partial substitution of NaCl by KCl could enhance beef myosin gel properties and heat-induced aggregation behavior

Foam-Mat Freeze Drying of Kiwiberry (Actinidia arguta) Pulp: Drying Kinetics, Main Properties and Microstructure

The kiwiberry is an interesting source of bioactive compounds (micronutrients, polyphenols vitamins and pectins) and enzyme actinidine but has limited durability. The aim of this study was to determine the impact of shelf temperature (10 °C, 25 °C and 40 °C) during freeze drying on the foam-mat kiwiberry pulp drying process and the quality of the obtained material based on analyses such as moisture content, water activity, hygroscopicity, solubility, microstructure and spectral measurement using the FTIR method. The use of higher shelf temperatures during freeze drying positively influenced the drying process, reducing its duration by up to 40.7%. The shelf temperature caused changes in the dry matter content (97.2–99.6%), water activity (0.159–0.221), structure and hygroscopic properties (1.41–4.41 g water/100 g d.m.) of the kiwiberry foam mats. Foam-mat drying at 40 °C exhibited a significantly lower water activity, total porosity and hygroscopicity, providing properties favorable for good microbiological and functional stability during storage. Furthermore, this temperature applied during freeze drying resulted in an increase in the solubility of the obtained material, which indicates its possible use in the matrix of other food products.

Regional and seasonal partitioning of water and temperature controls on urban dryness variability in China

Rapid urban expansion can significantly alter urban microclimate. In recent years, the phenomenon of urban dry islands (UDIs) has attracted increasing attention, but there remains a lack of understanding regarding the impact of urbanization on UDIs in different urban agglomerations (UAs). In this study, vapor pressure deficit (VPD) was used as the humidity indicator, and meteorological data from 20 UAs were used to explore how atmospheric temperature and humidity control urban dryness variability in China. Our analysis revealed that VPD trends are increasing with a median rate of 0.121 hPa/decade and 0.103 hPa/decade for urban and rural areas, respectively. The urbanization effect is more significant in coastal UAs. Furthermore, the difference in VPD between urban and rural areas obviously varied on a seasonal scale, with the largest difference observed in summer (i.e., −0.63 hPa on average and up to 2.66 hPa). Notably, our findings suggest that changes in local atmospheric water content primarily control the response of VPD to urbanization. In summer, the average contribution of temperature is 0.118 hPa, while the average contribution of relative humidity is 0.723 hPa. Overall, these results have important implications for our understanding of the impacts of urbanization on local climate.

Unraveling dynamic protein structures by two-dimensional infrared spectra with a pretrained machine learning model

Dynamic protein structures are crucial for deciphering their diverse biological functions. Two-dimensional infrared (2DIR) spectroscopy stands as an ideal tool for tracing rapid conformational evolutions in proteins. However, linking spectral characteristics to dynamic structures poses a formidable challenge. Here, we present a pretrained machine learning model based on 2DIR spectra analysis. This model has learned signal features from approximately 204,300 spectra to establish a "spectrum-structure" correlation, thereby tracing the dynamic conformations of proteins. It excels in accurately predicting the dynamic content changes of various secondary structures and demonstrates universal transferability on real folding trajectories spanning timescales from microseconds to milliseconds. Beyond exceptional predictive performance, the model offers attention-based spectral explanations of dynamic conformational changes. Our 2DIR-based pretrained model is anticipated to provide unique insights into the dynamic structural information of proteins in their native environments.

Combined effects of polyethylene terephthalate and abamectin on enzymatic activity and histopathology response in juvenile zebrafish (Danio rerio).

One of the most pressing global environmental issues is the widespread abundance and distribution of microplastics (MPs). MPs can act as vectors for other contaminants in the environment making these small plastic particles hazardous for ecosystems. The presence of MPs in aquatic environments may pose threats to aquatic organisms that ingest them. This study examined effects of abamectin (ABM) and polyethylene terephthalate (PET) MP fragments on histopathological and enzymatic biomarkers in zebrafish (Danio rerio). Zebrafish were exposed for 96 h to pristine PET-MPs at concentrations of 5 mg/L and 10 mg/L, ABM alone at 0.006 mg/L, and the same concentration of ABM in the presence of PET-MPs in aquaria. Histopathological analysis revealed tissue content changes in liver and kidney in the presence of ABM individually and in combination with MPs. Results of enzymatic analysis showed that MPs increased the bioavailability and toxicity of pesticides due to inhibition of catalase (CAT) and acid phosphatase (ACP) enzymes. However, MPs did not affect the toxicity of ABM for glutathione s-transferase (GST) enzyme. Despite the inhibition of acetylcholinesterase (AChE) in MPs or ABM treatments, and some neurotoxicity, no change in activity of this enzyme and neurotoxicity was observed in the combined MPs and ABM treatments, although toxicity effects of MPs and ABM on zebrafish require more detailed studies.

Influence of solute content and cooling rate on the preparation of semi-solid slurries for novel creep-resistant Mg-Gd-Ca-Zr alloys

The semi-solid Mg-Gd-Ca-Zr alloys slurries with fine and spherical α-Mg particles were successfully prepared. The influence of solute Gd and Zr contents, and cooling rate on the morphological evolution of α-Mg are systematically investigated. The results showed that addition of Zr will turn the coarse dendritic α-Mg particles into fine and globular particles. With the increase of Zr addition from 0 to 0.6 wt%, the particle size decreases, and the particle density and shape factor increase continuously. With the change of solute Gd content from 5 to 15 wt% in the Mg-yGd-2Ca-0.6Zr alloy, the particle size of α-Mg particles decreases continuously, and shape factor increases continuously. With cooling rate decreases from 3 to 1.5 °C/min, the α-Mg particles are slightly coarsened and the morphology are kept nearly globular, while with further decreasing into 0.5 °C/min, the α-Mg particles are severely coarsened and turned into irregular shape. The optimal Zr addition is ∼0.6 wt% and Gd addition is no less than 10 wt%, and the optimal cooling rate is 1.5–3 °C/min. The mechanisms for obtaining fine and spherical α-Mg particles through Zr and Gd additions are discussed to be different roles of heterogenous nucleation effects and growth restriction effects.

Voriconazole and Beauvercin Loaded Niosomal Mucoadhesive Nanogel as an effective Treatment for the Management of Oral Candidiasis

Oral candidiasis is a fungal infection, which affects the oral mucosa. The main causative agent of this is Candida albicans and as the literature indicates highest prevalence of Candida bloodstream infections in India. Most commonly azole derivatives such as miconazole, fluconazole, itraconazol etc. are used to manage the infection. However, their efficacy is compromised due to multidrug resistance. A new azole derivative voriconzole could be effective but its poor water solubility and bioavailability are seen as significant limitations. Its efficacy can be enhanced by combining it with beauvericin, a cyclic hexadepsipeptide with remarkable antifungal activity against candida. One study reported that combining of beauvericin with voriconzole reduce the MIC of voriconazole by 128th times. Furthermore the solubility and bioavailability were enhanced by loading both the drugs in niosomes. The niosomes were imcorporated into a polymeric mucoadhesive gel post statistically optimization by the Box-Behnken experimental design. The niosomes were subjected to evaluation for vesicular size, surface morphology, entrapment efficiency, drug release and zeta potential High entrapment efficiency was observed in the optimized noisome formulation F3 (89.89±0.64 % for voriconazole and 92.98±0.35 % for beauvericin) exhibiting cumulative drug release of 79.98±1.87 % for voriconazole and 69.17±1.98 % for beauvericin. The mucoadhesive gel was smooth, homogenious with desired pH and mucodhesion. The stability studies indicated that all the formulations are stable as evidence by no significant change in drug content over the time. These findings suggest that a niosmal mucoadhesive gel loaded with voriconazole and beauvericin is a good therapeutic delivery system for the effective treatment of oral candidiasis.

Physiological Mechanisms of BvCPD Regulation in Sugar Beet Growth

Sugar beet is an important sugar crop, and its roots are mainly used for processing raw materials to produce products such as sugar, molasses, and saccharin, as well as being used as fodder for livestock. BvCPD, a key enzyme gene for brassinosteroid (BR) synthesis, regulates the development of parenchyma cells and vascular bundles by promoting BR synthesis, which promotes the expansion of the sugar beet taproot and influences the growth, development, and yield of sugar beets. This study investigated the impact of BvCPD on the physiological metabolism of sugar beet utilizing BvCPD overexpression, silent, and wild-type (WT) lines. BvCPD increased the chlorophyll content and maximum photochemical efficiency and improved the photosynthetic characteristics of sugar beet leaves. Simultaneously, BvCPD increased the rate of sugar beet taproot respiration and ATP content by enhancing the activities of phosphoglycerate kinase, alcohol dehydrogenase, sucrose synthase, and sucrose synthase catabolism. Moreover, BvCPD induced changes in the sugar fraction content, which increased the sugar yield of a single plant. In addition, BvCPD promoted water absorption, nitrogen accumulation, and lignin/cellulose synthesis activities, facilitated by increased activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, cellulose synthase, and protein serine/threonine phosphatases, providing the requisite energy and materials for sugar beet growth. These findings not only provide a new perspective for understanding the physiological mechanisms regulating the growth of sugar beets but also provide a theoretical basis for the future improvement of sugar beet varieties through molecular breeding techniques.

New approaches in pear preservation: Putrescine and modified atmosphere packaging applications to maintain fruit quality during cold storage

AbstractPear (P. communis L.), which is a climacteric fruit species, has a very short storage and shelf life, and significant losses occur due to high metabolic activity and the fruit's respiration rate after harvest. Therefore, preventing or reducing post‐harvest quality losses in pear is one of the most basic problems awaiting solution. In this study, we planned for this purpose; the fruits of the Ankara pear cultivar treated with modified atmosphere packaging (MAP), putrescine (1 mM), and MAP + putrescine were stored for 120 days at 1°C and 90 ± 5% relative humidity. The quality analyses and measurements, such as weight loss, decay rate, fruit firmness, soluble solids content (SSC), titratable acidity (TA), total phenolic compounds, antioxidant capacity, organic acids, and specific phenolic compounds, were performed on the 30th, 60th, 90th, and 120th days. Weight loss and decay ratios were lower for putrescine and putrescine + MAP‐applied fruit. With these applications, the softening of the fruit was slowed down, and the increasing SSC in the fruit and the decreasing TA rates were lower, and thus the ripening of the fruit was delayed. Changes in individual phenolic content and organic acids were lower in MAP and putrescine‐applied fruit. The study revealed that MAP and putrescine applications in pear can be used effectively to maintain fruit quality after harvest.

The effect of the fatty acid composition of fried oil on the oil-absorbing capacity of tofu puffs

The influence of the fatty acid composition of fried oil on the oil-absorbing capacity of tofu puffs was examined. Two edible vegetable oils and three blends of different oleic acid and linoleic acid ratios were analyzed before and after frying, along with the rheological properties, fatty acid composition, acid value, peroxide value, and p-anisidine value. As well as the moisture content, oil content, and microstructure changes of the tofu puffs, were evaluated. The results revealed that the viscosity of all fried oils increased after short-time thermal exposure, while the acid value, peroxide value, and p-anisidine value also increased. The blend oil, with an oleic acid and linoleic acid ratio of 1:1, demonstrated good oxidation stability and low viscosity. The tofu puffs fried with this blend oil had lower oil content and weak adhesion, attributed to their microstructure. The development of special frying oil with good stability based on fatty acid composition provides a theoretical basis for the production of low-oil frying food.

Investigation of Changes in the Polyphenol Profile Verified by LC-MS/MS and the Pro-Health Activities of Fruit Smoothie.

Bilberry, bananas, and apples are used for smoothie production because the health-promoting activities and to prevent human diseases including neurodegenerative disorders. The smoothie is prepared to promote a promising practice for increasing the intake of fruit in the diet. The smoothie is packed into dark glass jars, pasteurized, and stored for up to 4 months at 4 or 22°C. Then, it is analyzed for the polyphenols profile using liquid chromatography-high resolution mass spectometry (LC-HRMS)Polyphenols content and the antiinflammatory, anticholinesterase, and antioxidant activities, and the impact on catalase activity are controlled using biochemical analyses. A significant decrease in the flavanol content (p < 0.05) is investigated, while there are lower decreases or no changes in the other polyphenols content in the smoothies stored at 4°C. The changes in the anticholinesterase and antioxidant activities of the smoothie are correlated with the total polyphenols, anthocyanins, flavonols, and tannins content. The proposed preservation of the smoothie and its storage at refrigeration temperature is adequate to maintain the smoothie's nutritional and functional effect for a 4-month shelf life. Even significant changes in the content of individual subgroups of polyphenols are not drastically reflected in the decrease of the smoothie biological activities.

Changes in body proportions and body fat content in GH-deficient and non-GH-deficient children during the first year of growth hormone treatment.

Introduction: The growth hormone/insulin-like growth factor-1 axis (GH/IGF-1) is crucial for longitudinal bone growth and exerts several metabolic effects. It is debatable whether and how the recombinant human growth hormone (rhGH) treatment affects the trunk-limb proportions. Aim of the study: We aim to evaluate the changes in body proportions and body fat content in short children with growth hormone deficiency (GHD), children born small for gestational age (SGA), and girls with Turner syndrome (TS) during the first year of rhGH therapy. Material and methods: The data of 70 children with GHD, 40 children born SGA, and 36 girls with TS from 1998 to 2019 were analyzed. The following parameters were measured: height, body weight, sitting height, and two skinfolds on the arm above the triceps brachii and below the scapula at the beginning of rhGH therapy and after the first year of treatment. Sitting height and lower limb length were presented as percentages (%) of body height. Fat mass percentage (%FAT) was calculated using the Slaughter formula. Results: At the beginning of rhGH, TS girls had the greatest height deficit (-2.7 SDS), the highest sitting height (%), and the lowest lower limb length (%) compared to children with GHD and children born SGA. Moreover, TS girls had higher body weight SDS, BMI SDS compared to SGA children (p < 0.001), and higher %FAT compared to both GHD and SGA children (p < 0.001 for both). After the first year of rhGH therapy, a significant increase in lower limb share (%) and a decrease in %FAT were observed in all the study groups. TS girls still had significantly higher sitting height (%), shorter lower limbs (%), and higher %FAT, body weight SDS, and BMI SDS compared to children with GHD and children born SGA (p < 0.05 for all variables). Conclusions: Our results show that rhGH treatment could increase lower limb length in relation to height and reduce fat mass in treated children. Girls with TS had the largest baseline body disproportions and the highest body fat content. Despite a satisfactory reduction in height deficit, after the first year of rhGH therapy, these girls had still higher body weight SDS, BMI SDS, %FAT, the highest sitting height (%), and the lowest lower limb length (%) compared to children with GHD and born SGA.

Challenges of e-library in developing countries higher educational institutions

Higher educational institutions in developing countries around the globe have come under rigorous criticism in the current era due to the poor quality of their products. Presently, there is an urgent need for higher education institutions in developing countries to catch up with innovations in teaching, learning, and research practices in developed economies. This paper explores the objectives, Information resources, basic requirements for accessing electronic library resources, advantages and disadvantages of E-library as well as the role of the use of Electronic Library in Higher Educational Institutions in Developing Countries. It also so examines the challenges associated with the use of electronic Libraries in higher educational Institutions in Developing Countries. These include the unstable power supply, Inability of vendors to supply the needed hardware, Change of contents, Lack of Digitization, Unified agreement, Economy, Network infrastructure, and Operational hours among others. It highlights the fact that electronic libraries are vital for efficient teaching, research, and learning in higher educational institutions of developing countries in the 21st century and as a result are a main concern. On the other hand, the paper stresses that to address the Challenges Associated with the Use of Electronic Library in Higher Educational Institutions in Developing Countries, the government at all levels should collaborate in funding libraries and make them more open to researchers’ needs in the country, the e-library should be equipped by employing professionals in the field of librarianship with ICT technical know-how that can give quality contributions to national development in spheres of life, among others.

Soil functional indicators in a semi-arid environment change patchy under the influence of shrub species

The presence of mountain shrublands within semi-arid regions is of great importance due to their function in the preservation of water and soil resources and their contribution to the conservation of biodiversity. The study of the effects of semi-arid highland shrubs on soil functional indicators has not yet received sufficient attention. To fill this knowledge gap, we conducted a study to investigate the effects of different shrub species specifically Carpinus orientalis Miller, Berberis integerrima Bunge, Crataegus microphylla C. Koch, Prunus spinosa L., and Rhamnus pallasii Fisch. and C. A. Mey) on soil physical, chemical, and biological properties. For this study, 15 individuals of each of the above-mentioned shrub species were selected. Soil samples were collected at a depth of 10 cm and an area of 30 cm × 30 cm beneath the canopy of these particular species. The examination of the influence of various independent factors on the variability of soil microclimate and biological characteristics indicates that the presence of shrub covers is the primary determinant of changes in water content, fauna and microflora population, microbial respiration and biomass, whereas soil temperature, MBN/MBP and qCO2 are more affected by season factor. Based on the biological fertility index, all the studied shrub covers are in the medium class with the maximum score under Carpinus shrub. Results indicate that soil biological characteristics play a more significant role in distinguishing in terms of soil functional indicators. Based on hot spots output, the soil functional indicators decreased in order of Carpinus > Crataegus > Berberis > Prunus > Rhamnus. As a conclsuion, Carpinus create hot spots (areas) of soil functional indicators in the mountainous ecosystems that claimed the potentially role of nutrient-rich soil cycles.

Research on the lubricating oil leakage characteristics in the vapor compression heat pump system under refrigerant leaking

Refrigerant leakage is a situation that often occurs in refrigeration equipment such as heat pumps. However, certain alternative refrigerants, while exhibiting superior environmental performance, possess varying levels of flammability. The leakage of flammable refrigerants into confined spaces can pose a significant risk of explosion. The amount of lubricant in the leaking refrigerant will undoubtedly affect the flammability characteristics of the refrigerant. In this paper, the content of PVE32 oil in the leaking refrigerant during the operation of a heat pump was investigated. The results showed that, under the five leakage rate scenarios set up in the experiment, the oil concentration was highest when the leakage occurred at the condenser inlet and lowest when the leakage was at the evaporator outlet. During the leakage process of the experiment, as the mass flow rate of the leakage increased, the oil content in the early stage of the leakage increased, but the oil content in the late stage of the leakage decreased. The reasons for the change in oil content were analyzed by the oil circulation rate and the degree of phase separation. The decrease of the oil circulation rate and the change of the degree of phase separation were the key factors affecting the magnitude of the oil content as the leakage proceeded. The results of the study reveal the leakage characteristics of lubricating oil with refrigerant leakage.

FEATURES OF TERRITORY MANAGEMENT DURING MARITAL STATE IN UKRAINE

The article discusses the current problems of territory management in the conditions of the Russian-Ukrainian war, which has been going on for two years. A description of the real state of territorial communities is offered, their problems caused by the war are outlined. A cluster model of the distribution of territorial communities depending on the situation during the war is presented. Possible ways of solving the main problems arising in the management of territories during martial law are determined. The article emphasizes that Russia's war against Ukraine, which has been going on for two years, has become the sharpest and most dangerous test of Ukrainian society since independence. Virtually all state and public institutions and organizations, government and executive institutions underwent significant changes in form and content. This especially applies to territorial communities, which, despite their short existence, have actually become the centers of the reconstruction of Ukrainian civil society on the lines of martial law. Territorial communities shouldered the main burden of volunteer assistance to the Armed Forces of Ukraine and territorial defense, reception and accommodation of forced migrants, provision of humanitarian aid, educational, medical, legal, communal services, organization of business and employment, as well as mobilization work. The article notes that under the conditions of martial law, the forms and content of territory management activities, in particular municipal management bodies, have changed: military-civilian administrations have appeared in regions and former districts. During the two years of the war, the functions and boundaries of the administrative activities of local self-government bodies were agreed upon, taking into account the challenges that were caused by the full-scale aggression of Russia. The article notes that the burden of the war had the greatest impact on the stability and vitality of territorial communities. Despite the fact that today it is generally possible to positively evaluate the administrative-territorial reform that was carried out on the eve of the war, territorial communities are in unequal conditions of life, have different possibilities of functioning, and require management decisions that are adequate to the real situation. The article emphasizes that the primary task at the moment is to improve relations between the central government and local self-government bodies, taking into account the actual military, economic, demographic, social, political, and humanitarian situation. We are talking about a significant expansion of the powers of territorial communities in all spheres of social life. It is impossible to rule in wartime by peaceful methods. This is all the more important because the administrative and territorial reform depends on the results at the front.

From the rhizosphere to plant fitness: Implications of microplastics soil pollution

Microplastics (MPs) are a widespread contaminant in the terrestrial environment, with potential impacts on the soil-plant system not yet well understood. This study explores the effects of oxidised low-density polyethylene-MPs (LDPE-MPs) on the rhizosphere ecology and plant fitness of Fragaria x ananassa (Duchesne ex Weston) Duchesne ex Rozier. The rhizospheric microbial community was investigated under the influence of 0,5 % LDPE-MPs by internal transcribed spacer (ITS) and 16 S rRNA metagenomic analysis; photosynthetic parameters, antioxidant enzyme activities, and nutrient accumulation were assessed to evaluate plant physiological and biochemical status. Genes related to jasmonic acid (JA), ethylene biosynthesis, and nitrate signalling pathways were analysed to define the plant molecular response. Our results showed a shift in the rhizosphere microbial community. We identified MPs molecular biomarkers in the contaminated rhizosphere (Fusarium, Thanatephorus and Pseudallescheria) with potential pathogenic functions and two novel molecular biomarkers (Ohtaekwangia and Ascobolus). MPs pollution negatively impacts plant fitness, which showed decreased chlorophyll a and b (40 and 48 %, respectively), a change in nutrient content (fluctuations between 14,42 and 26,7 %) at the leaf level and increased activity of antioxidant enzymes. Gene expression related to JA, ethylene biosynthesis, and nitrogen signalling pathways is enhanced in plants grown in contaminated soil, as well as the root endophytic and epiphytic microorganism interactions. Our results demonstrate that MPs pollution influences the rhizosphere microbial community and functions, and consequently, negatively impacts plant health.

Use of Directed Lactic Fermentation to Obtain Plant-Based, Upcycled Beverage from Milk Thistle Endosperm

This study aimed to evaluate the possibility of using milk thistle endosperm (MTE) extract with a protein content of &gt;2 g/100 mL to obtain a fermented product, an alternative to milk beverages. Directed lactic fermentation with Lacticaseibacillus rhamnosus was carried out. The course of the process was monitored. The changes in pH, the saturation of the medium with dissolved oxygen, and temperature were measured. The values of the main nutritional parameters, silymarin content, amino acid profile/content, and the PDCAAS value (Protein Digestibility-Corrected Amino Acid Score) were determined in the extract before and after fermentation. The lactic acid fermentation of the MTE extract took place in four phases, with the activity of L. rhamnosus being the most intense between 4 and 8 h into the process. As a result of fermentation, there were changes in the total amino acid content in the tested medium, suggesting the catabolism of aspartic acid, arginine, proline, and isoleucine via LAB took place. Particularly significant was the increase in the content of lysine and valine (4.95 and 4.68 g/100 g of total AA, respectively), which are the limiting amino acids in MTE. Although MTE contained approximately 1% silymarin, no presence of this flavonolignan complex was found in the extracts.