The oxygen cost of running at submaximal workloads is influenced in large part by individual differences in running mechanics. Several recent studies suggest that chronic altitude training may cause changes in submaximal oxygen uptake in elite endurance runners. Whether any change in running economy after altitude training is due to altered running mechanics is undetermined. PURPOSE: To determine if potential running economy changes after altitude training can be explained by changes in biomechanical factors. METHODS: Six elite male distance runners (VO2max 70.6 ± 4.5 mL/kg/min) completed a 28 day altitude training intervention in Flagstaff, Arizona, elevation 2150m, following a "live high - train low" training model. Running economy and gait parameters were measured 2-9 days prior to departure to altitude and 1-2 days after returning to sea level. Running economy was determined from VO2 measured in the final minute of 3 minute stages at 3 constant submaximal treadmill speeds of 291, 301, and 311 m/min. To measure variables related to running mechanics, subjects completed separate 30 second stages at constant treadmill speeds of 300, 315, 330, 345, and 360 m/min. Each running stage was separated by a period of standing rest. Wireless tri-axial 10g accelerometer devices, sampling at 1024 Hz, were securely attached to the laces of each shoe. Values of ground contact time, stride time, swing time, stride length, and stride frequency were determined from accelerometric output corresponding to foot strike and toe-off events obtained from a minimum of 25 consecutive steps. RESULTS: Post-altitude VO2 was higher at each submaximal workload (DVO2 pre- to post-altitude range 2.3 - 3.2 mL/kg/min; p range 0.037 - 0.115; n = 6). No significant differences were found in ground contact time, stride time, swing time, stride length or stride frequency at any speed after altitude training. CONCLUSIONS: Changes in running economy in elite endurance runners after chronic altitude training are not due to altitude mediated changes in running mechanics. Supported by the AAU/Bell-Updyke-Willett Kinesiology Research Fund, Indiana University School of HPER, and a grant from the High Performance Division, USA Track and Field.