ObjectiveTo analyze the beneficial role of novel jasmine titania tooth whitening gel on the color, surface roughness and morphology of human tooth enamel, concerning age. MethodsGreen dentistry incorporating Jasmine flower extract was used to fabricate titania (titanium oxide) nanoparticles whose physicochemical characteristics were evaluated through X-ray diffraction spectroscopy, scanning electron microscopy, dynamic light scattering spectroscopy, atomic force microscopy and energy dispersive X-ray spectroscopy in order to produce a novel jasmine tooth whitening gel. This whitening gel was applied on maxillary central incisors ranging between 20 and 50 years, which were investigated for color change, surface roughness and morphology. ResultsThe titania nanoparticles depicted 100 % pure anatase phase, 30.3 nm size and a spherical shape with no impurities. The color change (whitening) was found to be maximum in tooth enamel from the youngest age group (20 years), but minimum for the oldest age group (50 years) (p = 0.001). No significant change in surface roughness or morphology of tooth enamel belonging to the youngest age group (20 years) (p = 0.111) and middle age group (p > 0.05) was observed, but it was statistically significant in the teeth belonging to the ultra-middle age group (40 years) and the oldest age group (50 years) (p = 0.001). ConclusionNovel jasmine titania tooth whitening gel containing titania (titanium oxide) nanoparticles prepared by jasmine flower extract revealed potent whitening effects in all the age groups without affecting their surface roughness or morphology. Clinical significanceThe latest trends emerging in aesthetic dentistry show the demand for cosmetic procedures, especially tooth whitening, is enhanced. This novel jasmine titania tooth whitening gel was potent enough to whiten the enamel belonging to different age groups without enhancing the surface roughness or adversely affecting the surface morphology.