Changes In Quality Parameters Research Articles

Rapid and safe electrochemical disinfection of salt water using laser-induced graphene electrodes

Bacterial infections account for one of the major causes of disease losses in aquaculture. Antibiotics are the most common method used to mitigate these infections, but over the last few decades this has given rise to antimicrobial resistance so finding alternatives to these treatments is imperative. Here we report a drug-free cost-effective method for rapid and safe water disinfection. A pair of laser-induced graphene (LIG) electrodes charged with low voltage (2 V) rapidly inactivated Escherichia coli and Bacillus subtilis in various circulating saltwater systems without significant changes in water quality parameters (pH, dissolved oxygen, and temperature). Bacterial inactivation was enhanced with increasing water salinity during electrochemical disinfection using LIG electrodes. Meanwhile, the concentrations of oxidants such as hydrogen peroxide and ozone were generally low regardless of water salinities, and chlorine was not detectable during the treatments. No health impacts were observed in Japanese medaka exposed to eight days of 1 h LIG electrochemical treatments applied twice a day. Our findings suggest that rapid disinfection of saltwater could be achieved using LIG electrodes without negative health impacts on fish, providing potentially an efficient and safe method for controlling bacteria in saltwater systems.

Toxicity Assessment of the Ethanolic Stem Bark Extracts of Ackee, Blighia sapida on the African Catfish, Clarias gariepinus

Acute toxicity effects of ethanolic stem bark extract of ackee, Blighia sapida was tested on catfish fingerlings over a 96hr exposure period as a potent organic piscicide. A static toxicity bioassay was conducted after a preliminary trial tests were carried out to determine effective extract concentrations to be used. A 300 number catfish fingerlings were randomly selected and distributed in triplicate concentrations of stem bark extracts of ackee. The test fish were treated with concentrations of 0.00, 1.60, 3.20, 6.40 and 12.8g/l stem bark extracts. At the end of the exposure period, plant toxicant caused visible behavioral changes on test fish which included aggression, erratic swimming, stunned posture, opercula movement, air gulping, settlement at the bottom and eventual death. The LC50 lethal concentration of 8.394g/l was recorded while the safe concentration was established to be 1.281g/l. There were significant changes in water quality parameters of the experimental tanks. It could be inferred from the results of this study that treated fish were irritated by the plant toxicant such that unstable behavioral changes were exhibited in all treated groups. Therefore, the use of stem bark of ackee in fish harvesting should be regulated.

Unraveling the evolution of dissolved organic matter in full-scale A/A/O wastewater treatment process using size exclusion chromatography with PARAFAC and nonnegative matrix factorization analysis

Changes in spectral features and molecular weight (MW) of dissolved organic matter (DOM) along the A/A/O processes in eight full-scale wastewater treatment plants (WWTPs) were characterized using size exclusion chromatography with a diode array detector, a fluorescence detector and an organic carbon detector in tandem (SEC-DAD-FLD-OCD) as well as bulk water quality parameters. The parallel factor (PARAFAC) and the nonnegative matrix factorization (NMF) analyses have been effectively applied to the postprocessing of SEC-FLD fingerprints and SEC-OCD chromatograms, respectively. Individual SEC-FLD-PARAFAC or SEC-OCD-NMF components may span a broad range of MW, indicating that these SEC fractions within the same component were cognate and varied coherently across the dataset samples. The SEC-FLD-PARAFAC modeling and SEC-OCD-NMF analysis have clearly and concisely presented that the dramatic decreases of dissolved organic carbon, UV absorbance at 254 nm and protein-like fluorescence at Ex280/Em350 nm in the anaerobic process were primarily associated with the degradation of the large MW proteinaceous and polysaccharide-like biopolymers. It has also revealed that fluorescence of humic acid-like fractions increased significantly during the anaerobic process, but fluorescence of fulvic acid-like and humic substances’ building blocks decreased slightly. Laboratory experiments further confirmed the presence of the humification process in anaerobic processes, and the formation of humic acid-like fluorophores may be associated with carbohydrate metabolism. The combination of SEC-FLD-PARAFAC and SEC-OCD-NMF helped to establish the links between changes in bulk water quality parameters and the evolution of SEC MW fractions, which provides a more in-depth insight into wastewater DOM treatability and enables the optimization of wastewater treatment processes.

Microcalorimetry as a tool to measure shelf-life at postharvest of green asparagus

ABSTRACT There are many methods to store and extend the postharvest life of asparagus spears, but their effectivity is measured by variables that do not reflect spears’ senescence. The objective of the experiment was to utilised isothermal microcalorimetry as a tool to objectively measure the quality loss of asparagus spears during postharvest storage. Asparagus spears cv. Brock were stored at 2°C and 5°C and 90% humidity for 16 days. Metabolic heat and metabolic efficiency of asparagus spears were measured during 16 days. Also, water status, total soluble solids (TSS%), titratable acidity (TA%), pH, sugars, and space colour variables were recorded. We found a determination coefficient of 0.79 and 0.87 between metabolic heat and respiration rate in whole spears stored at 2°C and 5°C by a polynomic regression. It was recorded a regression between metabolic heat and fructose (0.5) and glucose (0.67) at 2°C and fructose (0.79) and glucose (0.76) at 5°C and sugar content. We detected slight changes in quality parameters (pH, Acidity, TSS), weight loss, and turgor pressure during storage. It was concluded that parameters obtained through isothermal microcalorimetry were sensitive and reliable indicators to detect changes in postharvest parameters commonly used to evaluate changes in asparagus spears quality.

Histamine-degrading halophilic bacteria from traditional fish sauce: Characterization of Virgibacillus campisalis TT8.5 for histamine reduction

Traditionally produced fish sauce can contain significant amounts of histamine. In some instances, the histamine concentration may be well above the limit recommended by the Codex Alimentarius Commission. The aim of this study was to discover new bacterial strains capable of growing under the stressful environmental conditions of fish sauce fermentation and metabolizing histamine. In this study, 28 bacterial strains were isolated from Vietnamese fish sauce products based on their ability to grow at high salt concentrations (23% NaCl) and tested for their ability to degrade histamine. Strain TT8.5 showed the highest histamine-degradation (45.1 ± 0.2% of initially 5 mM histamine within 7 days) and was identified as Virgibacillus campisalis TT8.5. Its histamine-degrading activity was shown to be localized intracellularly and the enzyme is a putative histamine dehydrogenase. The strain exhibited optimal growth and histamine-degrading activity at 37°C, pH 7%, and 5% NaCl in halophilic archaea (HA) histamine broth. It also showed pronounced histamine-degrading activity in HA histamine broth when cultivated at temperatures of up to 40 °C as well as in the presence of up to 23% NaCl. After treatment with immobilized cells, 17.6–26.9% of the initial histamine in various fish sauce products were reduced within 24 h of incubation, while no significant changes in other parameters of fish sauce quality were observed after this treatment. Our results indicate that V. campisalis TT8.5 is of potential interest to be applied in histamine degradation of traditional fish sauce.

Early Impact of Laser Vision Correction (LVC) on the Stability and Quality of the Retinal Image

This retrospective comparative study analyzes the early postoperative impact of laser vision correction for myopia on the optical quality and stability of functional vision using a double-pass aberrometer. Retinal image quality and visual function stability were assessed preoperatively, one and three months after myopic laser in situ keratomileuses (LASIK) and photorefractive keratectomy (PRK) using double-pass aberrometry (HD Analyzer, Visiometrics S.L, Terrassa, Spain). The parameters analyzed included vision break-up time (VBUT), objective scattering index (OSI), modulation transfer function (MTF), and Strehl ratio (SR). The study included 141 eyes of 141 patients, of whom 89 underwent PRK and 52 underwent LASIK. No statistically significant differences were noted between the two techniques in any analyzed parameters at three months postoperatively. However, a significant drop was observed in all parameters one month after PRK. Only the OSI and VBUT remained significantly altered from baseline at the three months follow-up visit, with an increased OSI by 0.14 +/- 0.36 (p < 0.01) and a shortened VBUT by 0.57 +/- 2.3 s (p < 0.01). No correlation was found between the changes in optical and visual quality parameters and age, ablation depth, or postoperative spherical equivalent. The stability and quality of the retinal images were similar between LASIK and PRK at three months postoperatively. However, significant degradation in all parameters was found one month after PRK.

Ecological environment monitoring for sustainable development goals in Gomishan international wetland, Gorgan Bay, Caspian Sea.

Monitoring of coastal ecosystems is of particular importance for sustainable development and environmental management. Remote sensing, using satellite imagery, can play an important role in these studies. In this study, changes of qualitative parameters in Gomishan wetland and Gorgan Bay located in the southeast of the Caspian Sea were investigated using Landsat images. Based on the results of the range of chlorophyll-a and water temperature changes in both ecosystems, there was an increasing trend. Evaluating of Secchi disk (transparency) ranges showed a decreasing trend until 2018. Also, among the studied years, 2009 has the lowest fluctuations. Overall, the findings of this study showed that changes in water quality parameters of Gorgan Bay and Gomishan wetland had been affected by ecological conditions and human activities such as industrial-domestic and agricultural effluents. Also, satellite data in such coastal ecosystems at different time intervals have valuable results and can be given special attention for the study of similar ecosystems.

A sustainable biosorption technique for treatment of industrial wastewater using snail shell dust (Bellamya bengalensis).

Water, an essential commodity available to mankind, is constantly under pollution threat. Industries are one of the major causative factors for its poor quality and therefore all organisms depending upon it, directly or indirectly are affected by various life-threatening problems. Thus, the treatment of discharge waste into the freshwater ecosystem is the dire need of the hour. The objective of the study is valorization of discarded snail shells for treatment of industrial wastewater. In the present study, industrial wastewater was treated using snail shell dust obtained from Bellamya bengalensis to assess change in water quality parameters. Various physico-chemical parameters like pH, total dissolved solids, electric conductivity, dissolved oxygen, biological oxygen demand, chemical oxygen demand, calcium, magnesium, total hardness, chlorides, bicarbonates, orthophosphates, sulfates, nitrates, and ammonia-N were assessed after its treatment with snail shell dust. Based on the present observation, it was concluded that all studied parameters except dissolved oxygen showed a remarkable decline in concentration after treatment with snail shell dust at the rate of 15g per liter at the end of 4days. Moreover, increased dissolved oxygen concentration also endorsed an enhancement in water quality. Statistical analysis through Pearson correlation and indices, viz., WQI (Water quality index) as well as Nemerow's Pollution index when applied to the present data, also supported an improvement in the water quality. The findings thus endorsed the utilization of snail shell dust as an eco-friendly technique and can be substituted as a sustainable method for the treatment of industrial wastewater.

A climate-water quality assessment framework for quantifying the contributions of climate change and human activities to water quality variations

Water quality safety has attracted global attention and is closely related to the development of the social economy and human health. It is widely recognized that climate change and human activities significantly affect water quality changes. Therefore, quantifying the contributions of factors that drive long-term water quality changes is crucial for effective water quality management. Here, we built a climate-water quality assessment framework (CWQAF) based on climate-water quality response coefficients and trend analysis methods, to achieve this goal. Our results showed that the water quality improved significantly by 4.45%–20.54% from 2011 to 2020 in the Minjiang River basin (MRB). Human activities (including the construction of ecological projects, stricter discharge measures, etc.) were the main driving factors contributing 65%–77% of the improvement effect. Notably, there were differences in the contributions of human activities to water quality parameter changes, such as DO (increase (I): 0.12 mg/L, human contribution (HC): 66.8%), CODMn (decrease (D): 0.71 mg/L, HC: 67.2%), BOD5 (D: 1.10 mg/L, HC: 77.7%), CODCr (D: 4.20 mg/L, HC: 81.2%), TP (D: 0.13 mg/L,HC: 72.8%) and NH3–N (D: 0.40 mg/L, HC: 63.0%). Climate change explained 23%–35% of the variation in water quality. The water quality response to climate change was relatively significant with precipitation. For example, the downstream region was more susceptible to climate change than was the upstream region, as the downstream movement of precipitation centers strengthened the process of climatic factors affecting water quality changes in the MRB. Generally, although human activities were the main driving factor of water quality changes at the basin scale, the contribution of climate change could not be ignored. This study provided a manageable framework for the quantitative analysis of the influence of human activities and climate change on water quality to enable more precise and effective water quality management.

Performance of Bayesian Model Averaging (BMA) for Short-Term Prediction of PM10 Concentration in the Peninsular Malaysia

In preparation for the Fourth Industrial Revolution (IR 4.0) in Malaysia, the government envisions a path to environmental sustainability and an improvement in air quality. Air quality measurements were initiated in different backgrounds including urban, suburban, industrial and rural to detect any significant changes in air quality parameters. Due to the dynamic nature of the weather, geographical location and anthropogenic sources, many uncertainties must be considered when dealing with air pollution data. In recent years, the Bayesian approach to fitting statistical models has gained more popularity due to its alternative modelling strategy that accounted for uncertainties for all air quality parameters. Therefore, this study aims to evaluate the performance of Bayesian Model Averaging (BMA) in predicting the next-day PM10 concentration in Peninsular Malaysia. A case study utilized seventeen years’ worth of air quality monitoring data from nine (9) monitoring stations located in Peninsular Malaysia, using eight air quality parameters, i.e., PM10, NO2, SO2, CO, O3, temperature, relative humidity and wind speed. The performances of the next-day PM10 prediction were calculated using five models’ performance evaluators, namely Coefficient of Determination (R2), Index of Agreement (IA), Kling-Gupta efficiency (KGE), Mean Absolute Error (MAE), Root Mean Squared Error (RMSE) and Mean Absolute Percentage Error (MAPE). The BMA models indicate that relative humidity, wind speed and PM10 contributed the most to the prediction model for the majority of stations with (R2 = 0.752 at Pasir Gudang monitoring station), (R2 = 0.749 at Larkin monitoring station), (R2 = 0.703 at Kota Bharu monitoring station), (R2 = 0.696 at Kangar monitoring station) and (R2 = 0.692 at Jerantut monitoring station), respectively. Furthermore, the BMA models demonstrated a good prediction model performance, with IA ranging from 0.84 to 0.91, R2 ranging from 0.64 to 0.75 and KGE ranging from 0.61 to 0.74 for all monitoring stations. According to the results of the investigation, BMA should be utilised in research and forecasting operations pertaining to environmental issues such as air pollution. From this study, BMA is recommended as one of the prediction tools for forecasting air pollution concentration, especially particulate matter level.

Anion Exchange Resin and Inorganic Anion Parameter Determination for Model Validation and Evaluation of Unintended Consequences during PFAS Treatment.

When implementing anion exchange (AEX) for per- and polyfluoroalkyl substances treatment, temporal drinking water quality changes from concurrent inorganic anion (IA) removal can create unintended consequences (e.g., corrosion control impacts). To understand potential effects, four drinking water-relevant IAs (bicarbonate, chloride, sulfate, and nitrate) and three gel-type, strong-base AEX resins were evaluated. Batch binary isotherm experiments provided estimates of IA selectivity with respect to chloride ( ) for IA/resin combinations where bicarbonate < sulfate ≤ nitrate at studied conditions. A multi-IA batch experiment demonstrated that binary isotherm-determined values predicted competitive behavior. Subsequent column experiments with and without natural organic matter (NOM) allowed for the validation of a new ion exchange column model (IEX-CM; https://github.com/USEPA/Water_Treatment_Models). IA breakthrough was well-simulated using binary isotherm-determined values and was minimally impacted by NOM. Initial AEX effluent water quality changes with corrosion implications included increased chloride and decreased sulfate and bicarbonate concentrations, resulting in elevated chloride-to-sulfate mass ratios (CSMRs) and Larson ratios (LRs) and depressed pH until the complete breakthrough of the relevant IA(s). IEX-CM utility was further illustrated by simulating the treatment of low-IA source water and a change in the source water to understand the resulting duration of changes in IAs and water quality parameters.

Effect of amla and ginger powders on quality criteria of African catfish (Clarias gariepinus) fingers.

Catfish (Clarias gariepinus) meat isn't preferred by consumers in compared to other fish species meat, thus to enhance the use of catfish meat, ready to eat catfish fingers were prepared with some plants additives (amla and ginger powder). The mean objective of this study was to assess the impact of amla and ginger powder on catfish finger qualities in terms of physical, chemical, microbiological, and sensorial parameters under stored at a low temperature (5 ± 1 °C). The obtained results were compared with those of a control sample (basic formula) and a sample containing the synthetic antioxidant. During the storage period, the levels of pH, thiobarbituric acid, total volatile basic nitrogen, trimethylamine, total bacteria count, psychrophilic bacteria, molds and yeasts counts increased dramatically, although the values remained within acceptable ranges. The findings also revealed, that amla and ginger powder considerably (p < 0.05) reduced the changes in quality parameters, as well as there was a considerable increase in the quality parameter in all treated samples than in the control. Finally, amla and ginger powder can be a substitute for synthetic antioxidants and antimicrobials. These findings suggest that the powder of amla and ginger are suitable for use as a natural antioxidants and antimicrobials to extend the shelf-life of animal products.

Water and sediment changes and the eco-environment response characteristics in Three Gorges Reservoir after the impoundment

三峡水库举世瞩目,是长江保护与治理的关键性枢纽工程,水库自2003年蓄水运行后库区水沙条件均发生了显著改变,对库区水生态环境产生了深远影响。本文系统综述了三峡水库蓄水运行后库区的水流动力特性和泥沙淤积特性,总结了蓄水后水库的新水沙运动情况;从水库蓄水运行后水质参数特征,营养物质、重金属和典型有毒有机污染物的赋存特征等方面,分析了三峡水库库区新水沙变化条件下的水环境响应特征;基于水库蓄水前后浮游植物、底栖动物等重点生物群落的现存量和群落特征变化规律,探索了三峡水库新水沙变化条件下库区的水生态响应特征;最后对未来三峡水库运行下的水生态环境效应研究提出展望,本文可为三峡水库水生态环境保护科学研究和管理提供新的认识和决策参考。;The water-sediment conditions in the reservoir area had changed significantly after the operation of the Three Gorges Reservoir, which has had a profound impact on the water ecology of the reservoir area. In this paper, the dynamic characteristics of the water flow and sedimentation in the reservoir area before and after the operation of the Three Gorges Reservoir were summarized, and the changes in the water-sedimentation conditions after water storage were analyzed. The water environmental response characteristics under this newly formed water-sediment conditions were evaluated in terms of changes in water quality parameters, nutrients, heavy metals, and toxic organic pollutants after the reservoir storage. The ecological response characteristics of aquatic biotas were also explored based on the community changes of phytoplankton, benthic fauna and other key biological communities before and after the reservoir storage. Finally, the outlook for future research on the water ecological environmental effects of the Three Gorges Reservoir was proposed. This paper can provide support for the water ecological environment protection of the Three Gorges Reservoir.

Characterization of Fruit Quality Traits and Biochemical Properties in Different Myanmar Mango Cultivars during Ripening Stages

Here, we characterized the changes in fruit quality and biochemical parameters in four Myanmar mango cultivars from ripening stage 1 to 4 at ambient temperature. Total soluble solids, total sugars, and reducing and non-reducing sugar content increased, whereas titratable acidity decreased with increasing storage time in all cultivars. ‘Sein Ta Lone’ showed the highest consumer acceptability, with maximum sensory quality scores owing to its unique characteristics. ‘Hin Thar’ and ‘Ma Chit Su’ also had better quality and sensory attributes than ‘Yin Kwae’. Sugar/acid ratios in all cultivars ranged from 23 to 50, the standard sugar/acid ratios in high-quality mango fruits. The total phenolic content (TPC) and antioxidant activity among cultivars ranged from 8.20 to 14.96 mg gallic acid equivalents and 19.52 to 26.79 mg vitamin C equivalents antioxidant capacity, respectively, per 100 g of fruit extract throughout the storage. ‘Hin Thar’ was the richest in phytochemical compounds. A significant positive correlation was found between total phenolic activity and 2,2-diphenyl-1-picryl-hydrazyl free radical scavenging activity of fruits, showing that TPC exhibited linear relationships with the antioxidant activities of each mango variety during the different stages of ripening.

NOVEL INSIGHTS INTO GAIT, MOTOR CONTROL, AND THE BRAIN: IMPLICATIONS FOR COGNITIVE AGING

Abstract What can muscles, gait, and brain activity tell us about cognitive decline? Are there certain markers we can track that are early predictors of cognitive status years later? In the current symposia, our goal is to address these questions with recent pilot, longitudinal and cross-sectional studies that measure potential markers of cognitive decline in different older adult cohorts. The first speaker will present findings on changes in skeletal muscle adiposity and 10-year change in global cognition from the Health Aging and Body Composition (Health ABC) study. The second speaker explores cross-national comparisons of gait speed and its association with cognitive function from the Cohort Studies of Memory in an International Consortium (COSMIC). The third speaker investigates changes in brain activity with functional near infra-red spectroscopy (fNIRS) from single to dual-task walking and its relation to changes in several gait quality parameters. The fourth speaker presents pilot work that examines dual-task gait and tapping with fNIRS and compares the dual-task performance and brain activity of older adults who report experiencing subjective cognitive decline to those that do not. The final speaker presents findings from the Baltimore Longitudinal Study on Aging (BLSA) that demonstrate that early markers of slow gait and metabolic dysfunction could identify those at risk of progression to dementia 7 years prior to onset. Taken together, the findings from this symposium present novel markers of changes in cognitive function in older adults and ultimately targets for prevention or slowing of cognitive declines in older adults at risk for dementia.

Analysis of an Evaporative Condensation System Coupled to a Microchannel-Separated Heat Pipe for Data Centers

In the age of the digital economy, the data center is the most crucial piece of infrastructure. The issue of the excessive power consumption of a data center’s cooling system needs to be addressed as the national objective of “peak carbon and carbon neutrality” is increasingly promoted. In this study, a microchannel-separated heat pipe-cooling system with evaporative condensation is introduced. The system may switch between three modes of operation in response to changes in outdoor air quality parameters, thereby maximizing the utilization of natural cooling sources while lowering data centers’ cooling costs. The purpose of this paper is to analyze the energy-saving potential of the hybrid system through experimental tests. The results show that 114.4% is the ideal liquid-loading rate for the heat pipe system. Under working conditions in Xi’an, the annual operating hours of the three modes accounted for 47.2%, 6.1%, and 46.7%. The hybrid cooling system may save 62.04% of the energy used annually compared to the standard cooling system and the cooling system in the server room thanks to its yearly average COP of 9.43.

Cane Girdling Influence on the Berry Texture Properties of Three Table Grape Varieties

The effects of cane girdling on the berry texture characteristics were studied in three table grape cultivars. The application was carried out at véraison. The total soluble solids (TSS, °Bx) of berry must and berry weight were measured. The berry mechanical properties were investigated by a texture analyser. A double compression test was used to determine berry hardness and its derived parameters. A puncture test was also applied to assess skin hardness, skin elasticity and skin break energy. Skin thickness was also investigated. The sugar concentration of the must and the berry weight after the girdling treatment were significantly higher compared to those of the control. Berry hardness, skin hardness and skin thickness were significantly affected by this technique. The changes in berry mechanical and quality parameters with this application did not show the same pattern as those observed during control ripening. Notably, in our experiment, after the girdling treatments, the higher berry hardness was accompanied with a higher sugar concentration compared to the control. The skin hardness showed lower values as a result of girdling. However, skin thickness was significantly higher in the girdled berries compared to the non-treated vines.

Development of Ready-to-Eat Organic Protein Snack Bars: Assessment of Selected Changes of Physicochemical Quality Parameters and Antioxidant Activity Changes during Storage.

Novel organic high-protein bars (HPB) were developed and produced from organic ingredients such as prebiotic and pro-healthy additives or whey protein concentrate (WPC-80). The influence of temperature and time on the selected physicochemical parameters and antioxidant activity of three formulations of HPBs when stored (at 4 °C and 22 °C for 3 months) was investigated. The fresh products varied on the basis of available carbohydrates, crude lipids, amino acid profile, and fatty acid profile resulting from the used formulations. A total of 17 amino acids (AA), including 10 essential amino acids (EAA), were identified in HPBs. The concentrations of all essential amino acids determined by EAA scores (AAS), except Histidine (His), were higher than the FAO/WHO/UNU (2007) pattern; for the WPC-80 however, in the case of the developed HPB, the scores were lower (0.21-0.48). The first limiting amino acid in HPB was Val (Valine). The temperature and time of storage significantly affected the proximate chemical composition and an assessment of the products' antioxidant activity. The amino acid and fatty acid composition of stored products slightly changed. However, stored HPBs had a low content of trans fatty acids (TFAs). The optimal method of storage for the investigated bars was at the temperature of 4 °C for 3 months.

Physicochemical and histopathological parameters of broilers with dorsal cranial myopathy.

This study aimed to investigate the effect of dorsal cranial myopathy (DCM) on chicken meat quality. Sixty-six Ross 308 AP broilers, 47 days old, of both sexes, weighing about 3.51 kg, were slaughtered according to standard industrial practices, and evaluated for meat color, pH, chemical composition, collagen content, fatty acid profile, and histopathological parameters. Comparisons between normal and DCM-affected meat were performed using Student's t-test at the 5% significance level. Histological analysis of muscle tissues affected by DCM showed myofiber degeneration, proliferation of inflammatory cells, fibroplasia, and necrosis with fibrosis. DCM samples had lower protein content and higher moisture, ash, insoluble collagen, total collagen, and pH. DCM-affected meat was redder and more yellowish. There were no differences in lipid or soluble collagen contents between groups. DCM-affected meat had higher percentages of arachidonic acid (C20:4n-6) and eicosapentaenoic acid (C20:5n-3). This study revealed that DCM-affected meat exhibits considerable changes in quality parameters.

Ameliorative effect of silymarin on the quality of frozen-thawed boar spermatozoa.

Although Silymarin (SMN) has powerful antioxidant properties, little is known about its effects on the quality of frozen-thawed boar sperm. The present study aimed to evaluate the influences of SMN added to the thawing extender on boar sperm parameters essential for fertilization. The frozen-thawed semen was diluted in a Modena thawing extender supplemented with different concentrations of SMN (0, 5, 10, 20 and 50 μM respectively), and then the changes in quality parameters, antioxidant capacity, mitochondrial function and in vitro fertilization (IVF) capability of frozen-thawed sperm were assessed. Here we demonstrated that the motility, plasma membrane integrity and acrosomal integrity of frozen-thawed sperm improved efficiently by SMN (p < .05). In antioxidant parameters evaluation, the tROS level and MDA content of frozen-thawed spermatozoa were reduced in the 20 μM SMN group, while the T-AOC activity significantly increased (p < .05), indicating that the supplementation with SMN can promote the antioxidant capacity of frozen-thawed boar sperm. Besides, we also discovered that the addition of SMN significantly upregulated ATP content and enhanced the mitochondrial activity of sperm. More interestingly, SMN promoted the activities of mitochondrial respiratory chain complexes (MRCC) I, II, III and IV in frozen-thawed sperm significantly. Functionally, the higher penetration rate and increased total efficiency of fertilization were observed in the 20 μM SMN group. In summary, supplementation with SMN in the thawing medium ameliorates the quality of frozen-thawed boar sperm by enhancing mitochondrial respiratory capacity, producing large amounts of ATP and regulating ROS formation.