Changes In Physiological Parameters Research Articles

Changes in physiological parameters in healthy dogs on an underwater treadmill when the water level is set at the hip joint

Understanding internal and external loading in exercise is important for safe rehabilitation. The physiological parameters of dogs have been studied in swimming, but those of dogs walking on an underwater treadmill have not been documented. In this study, four healthy beagle dogs were examined for changes in physiological parameters before and after walking in water for 20 min at a speed of 4 km/h with the water level set at the height of the hip joint as an external load. Statistical analysis of the results was performed using paired sample t-tests. Results indicated that heart rate (125 ± 11.8 beat/min to 163 ± 9.5 beat/min) and lactate levels (2 ± 0.1 mmol/L to 2.4 ± 0.2 mmol/ L) significantly increased from before to after walking on an underwater treadmill. Additional research is needed on internal loading when using underwater treadmills, which would further contribute to the safety of rehabilitation.

Urethane provides an unparalleled anaesthetic model for natural sleep: Commentary on Mondino et al., 2022.

Urethane provides an unparalleled anaesthetic model for natural sleep: Commentary on Mondino et al., 2022.

Heat-Stress-Induced Changes in Physio-Biochemical Parameters of Mustard Cultivars and Their Role in Heat Stress Tolerance at the Seedling Stage

In the era of global warming, heat stress, particularly at the seedling stage, is a major problem that affects the production and productivity of crops such as mustard that are grown in cooler climates. Nineteen mustard cultivars were exposed to contrasting temperature regimes-20 °C, 30 °C, 40 °C and a variable range of 25-40 °C-and evaluated for changes in physiological and biochemical parameters at the seedling stage to study their role in heat-stress tolerance. Exposure to heat stress showed detrimental effects on seedling growth as revealed by reduced vigor indices, survival percentages, antioxidant activity and proline content. The cultivars were grouped into tolerant, moderately tolerant and susceptible based on the survival percentage and biochemical parameters. All the conventional and three single-zero cultivars were found to be tolerant and moderately tolerant, respectively, while double-zero cultivars were reckoned to be susceptible except for two cultivars. Significant increases in proline content and catalase and peroxidase activities were found associated with thermo-tolerant cultivars. More efficient antioxidant system activity and proline accumulation were noticed in conventional along with three single-zero (PM-21, PM-22, PM-30) and two double-zero (JC-21, JC-33) cultivars that might have provided better protection to them under heat stress than the remaining one single- and nine double-zero cultivars. Tolerant cultivars also resulted in significantly higher values of most of the yield attributing traits. Heat-stress-tolerant cultivars could easily be selected based on the survival percentage, proline and antioxidants at the seedling stage and included as efficient cultivars in breeding programs.

Mechanistic-mathematical modeling of intracranial pressure (ICP) profiles over a single heart cycle. The fundament of the ICP curve form

The intracranial pressure (ICP) curve with its different peaks has been comprehensively studied, but the exact physiological mechanisms behind its morphology has not been revealed. If the pathophysiology behind deviations from the normal ICP curve form could be identified, it could be vital information to diagnose and treat each single patient. A mathematical model of the hydrodynamics in the intracranial cavity over single heart cycles was developed. A Windkessel model approach was generalized but the unsteady Bernoulli equation was utilized for blood flow and CSF flow. This is a modification of earlier models using the extended and simplified classical Windkessel analogies to a model that is based on mechanisms rooted in the laws of physics. The improved model was calibrated with patient data for cerebral arterial inflow, venous outflow, cerebrospinal fluid (CSF), and ICP over one heart cycle from 10 neuro-intensive care unit patients. A priori model parameter values were obtained by considering patient data and values taken from earlier studies. These values were used as an initial guess for an iterated constrained-ODE (ordinary differential equation) optimization problem with cerebral arterial inflow data as input into the system of ODEs. The optimization routine found patient-specific model parameter values that produced model ICP curves that showed excellent agreement with clinical measurements while model venous and CSF flow were within a physiologically acceptable range. The improved model and the automated optimization routine gave better model calibration results compared to previous studies. Moreover, patient-specific values of physiologically important parameters like intracranial compliance, arterial and venous elastance, and venous outflow resistance were determined. The model was used to simulate intracranial hydrodynamics and to explain the underlying mechanisms of the ICP curve morphology. Sensitivity analysis showed that the order of the three main peaks of the ICP curve was affected by a decrease in arterial elastance, a large increase in resistance to arteriovenous flow, an increase in venous elastance, or a decrease in resistance to CSF flow in the foramen magnum; and the frequency of oscillations were notably affected by intracranial elastance. In particular, certain pathological peak patterns were caused by these changes in physiological parameters. To the best of our knowledge, there are no other mechanism-based models associating the pathological peak patterns to variation of the physiological parameters.

Individualized Housing Modifies the Immune-Endocrine System in CD1 Adult Male Mice.

In the last years, different research groups have made considerable efforts to improve the care and use of animals in research. Mice (Mus musculus) are the most widely used animal species in research in the European Union and are sociable and hierarchical creatures. During experiments, researchers tend to individualize males, but no consideration is given to whether this social isolation causes them stress. The aim of this study was, therefore, to explore whether 4 weeks of social isolation could induce changes in different physiological parameters in adult Crl:CD1(ICR) (CD1) males, which may interfere with experimental results. Body weight, blood cells, and fecal corticosterone metabolites levels were the analyzed parameters. Blood and fecal samples were collected at weeks 1 and 4 of the experimental procedure. Four weeks of single housing produced a significant time-dependent decrease in monocytes and granulocytes. Fecal corticosterone metabolite levels were higher in single-housed mice after 1 week and then normalized after 4 weeks of isolation. Body weight, red blood cells, and platelets remained unchanged in both groups during this period. We can, therefore, conclude that social isolation affects some immune and endocrine parameters, and that this should be taken into account in the interpretation of research data.

Machine learning models applied to predicate post activity oxygen saturation levels

Machine learning is a rapidly growing field with widespread application in various industries, including healthcare. In recent years, significant advancements in machine learning have enhanced our understanding of data analytics and prediction models in analyzing physiological data, such as blood oxygen levels, heart rate, and blood pressure. This paper focuses on physical activity in adolescence during the pandemic period. The study showed that going outside for a short walk can increase blood oxygen levels. Furthermore, the consumption of certain foods can also raise oxygen saturation levels. Maintaining a high blood oxygen level during exercise can improve athletic performance and reduce injury risk. However, during exercise, the blood oxygen level tends to decrease as the heart rate increases to supply more oxygen to the body. When the blood oxygen level drops too low during exercise, it can lead to fatigue, shortness of breath, and even fainting. By tracking heartbeats, blood oxygen levels, and other physiological parameters during exercise with the YAMAY Smart Watch wearable device, individuals can gain insights into their body's response to physical activity and adjust their exercise routine accordingly. After collecting data using wearable devices and mobile apps, machine learning algorithms can be trained to predict changes in physiological parameters of post-activity, providing valuable insights into the effectiveness of different exercises and identifying potential health risks. Our study used supervised machine learning classification algorithms to predict the expected data with the target data. We used K-fold cross-validation techniques to split the dataset into training, validation, and test sets for the supervised machine learning classification algorithms. After testing each of the machine learning models (K-Nearest Neighbor (KNN), Naïve Bayes, and Random Forest), it was found that the Random Forest had the best prediction accuracy of 98.75%. On the other hand, KNN had a poor prediction accuracy, lower than 41.1%. Therefore, the Random Forest model can accurately predict the effect of change on the oxygen saturation level during exercise.

Rhizobial symbiosis promotes drought tolerance in Vicia sativa and Pisum sativum

Drought is one of the greatest threats to agricultural production. Legumes are relatively susceptible to drought and have the unique ability to establish symbiosis with nitrogen-fixing bacteria. Deciphering how symbiotic interactions affect legume stress tolerance is a relevant question that remains unsolved, especially in the context of maintaining crop production under climate change. Our study addresses the comparative analysis of drought response and drought tolerance in symbiosis-dependent versus N-fed plants. Our results support the existence of attenuation in the damage promoted by drought in nodulated pea and vetch plants. This increase in drought tolerance correlates with changes in physiological parameters, such the hydric status of the plant, changes in biochemical parameters, such as the accumulation of osmolytes and antioxidant pigments, or the protection against lipid peroxidation. We have also found changes at the molecular level, identifying different genomic pathways through transcriptomic analysis. Although this characterization strengthens the evidence for a common strategy of coping with water deprivation in nodulated and N-fed plants, the above-mentioned changes suggest divergent and specific strategies of drought tolerance in nodulated plants as well as differences between pea and common vetch. In addition, our transcriptomic studies help unravel the molecular mechanisms through which rhizobial symbiosis contributes to drought tolerance in Vicia sativa and Pisum sativum. These results highlight the potential of using effective Rhizobium inoculants as a tool to cope with drought and enhance N-fixing ability under agriculturally relevant conditions.

Effect of menthol lozenges after extubation on thirst, nausea, physiological parameters, and comfort in cardiovascular surgery patients: A randomized controlled trial

ObjectivesTo determine the effect of post-extubation oral menthol lozenges on thirst, nausea, physiological parameters, and comfort level in patients undergoing cardiovascular surgery. Research methodology/designThe study was a single-centre, randomized controlled trial. SettingThis study included 119 patients undergoing coronary artery bypass graft surgery in a training and research hospital. Patients in the intervention group (n = 59) received menthol lozenges at 30, 60, and 90 min after extubation. Patients in the control group (n = 60) received standard care and treatment. Main outcome measuresThe primary outcome of the study was the change in post-extubation thirst assessed by Visual Analogue Scale after using menthol lozenges compared to baseline. Secondary outcomes were changes in post-extubation physiological parameters and nausea severity assessed by Visual Analogue Scale compared to baseline, and comfort level assessed with Shortened General Comfort Questionnaire. ResultsBetween-group comparisons showed that the intervention group had significantly lower thirst scores at all time points and nausea at the first assessment (p < 0.05) and significantly higher comfort scores (p < 0.05) than the control group. There were no significant differences between the groups in physiological parameters at baseline or any of the postoperative assessments (p > 0.05). ConclusionIn patients undergoing coronary artery bypass graft surgery, the use of menthol lozenges effectively increased comfort level by reducing post-extubation thirst and nausea, but had no effect on physiological parameters. Implications for clinical practiceNurses should be vigilant for complaints such as thirst, nausea, and discomfort in patients after extubation. Nurses’ administration of menthol lozenges to patients may help reduce post-extubation thirst, nausea, and discomfort.

Choroidal vascularity index (CVI)-Net-based automatic assessment of diabetic retinopathy severity using CVI in optical coherence tomography images.

A deep learning model called choroidal vascularity index (CVI)-Net is proposed to automatically segment the choroid layer and its vessels in overall optical coherence tomography (OCT) scans. Clinical parameters are then automatically quantified to determine structural and vascular changes in the choroid with the progression of diabetic retinopathy (DR) severity. The study includes 65 eyes consisting of 34 with proliferative DR (PDR), 17 with nonproliferative DR (NPDR), and 14 healthy controls from two OCT systems. On a dataset of 396 OCT B-scan images with manually annotated ground truths, overall Dice coefficients of 96.6 ± 1.5 and 89.1 ± 3.1 are obtained by CVI-Net for the choroid layer and vessel segmentation, respectively. The mean CVI values among the normal, NPDR, and PDR groups are consistent with reported outcomes. Statistical results indicate that CVI shows a significant negative correlation with DR severity level, and this correlation is independent of changes in other physiological parameters.

Fentanilo para la coinducción de la anestesia con propofol en perros premedicados con morfina

The aim of the present study was to evaluate the effectiveness of fentanyl to reduce the induction dose of propofol and the quality of induction in dogs premedicated with morphine. The sedative effect promoted by morphine and its action on some physiological variables were also investigated. Twenty-two healthy dogs had their baseline values (BL) of the physiological variables and the degree of sedation recorded. Then, received morphine (0.3 mg/kg) intramuscularly. Ten minutes after morphine administration (T10), all variables were reassessed. Dogs were randomly assigned to receive intravenously: fentanyl (2.5 µg/kg) or saline (3.0 mL), followed by propofol (2 mg/kg/minute), groups FP and SP, respectively. The dose of propofol required for intubation and the quality of anesthetic induction were recorded. Morphine administration resulted in vomiting and no sedation. Heart rate significantly decreased at T10 in FP and in SP groups, compared to BL. The total dose of propofol used for anesthetic induction was 6.4 ± 1.7 and 5.8 ± 1.6 [mean ± standard deviation] mg/kg in FP and SP, respectively, with no differences between groups (P &gt; 0.05). The quality of anesthetic induction score was excellent in all groups. During anesthetic induction, some dogs had apnea and muscle stiffness. Fentanyl was not effective in reducing the propofol requirement in the anesthetic induction of dogs premedicated with morphine. Anesthetic induction was excellent in both groups, and may be accompanied by apnea and muscle stiffness. Morphine resulted in no sedation, minimal changes in physiological parameters and vomiting.

Toxicity of tributyltin to the European flat oyster Ostrea edulis: Metabolomic responses indicate impacts to energy metabolism, biochemical composition and reproductive maturation.

Tri-Butyl Tin (TBT) remains as a legacy pollutant in the benthic environments. Although the toxic impacts and endocrine disruption caused by TBT to gastropod molluscs have been established, the changes in energy reserves allocated to maintenance, growth, reproduction and survival of European oysters Ostrea edulis, a target species of concerted benthic habitat restoration projects, have not been explored. This study was designed to evaluate the effect of TBT chloride (TBTCl) on potential ions and relevant metabolomic pathways and its association with changes in physiological, biochemical and reproductive parameters in O. edulis exposed to environmental relevant concentrations of TBTCl. Oysters were exposed to TBTCl 20 ng/L (n = 30), 200 ng/L (n = 30) and 2000 ng/L (n = 30) for nine weeks. At the end of the exposure, gametogenic stage, sex, energy reserve content and metabolomic profiling analysis were conducted to elucidate the metabolic alterations that occur in individuals exposed to those compounds. Metabolite analysis showed significant changes in the digestive gland biochemistry in oysters exposed to TBTCl, decreasing tissue ATP concentrations through a combination of the disruption of the TCA cycle and other important molecular pathways involved in homeostasis, mitochondrial metabolism and antioxidant response. TBTCl exposure increased mortality and caused changes in the gametogenesis with cycle arrest in stages G0 and G1. Sex determination was affected by TBTCl exposure, increasing the proportion of oysters identified as males in O. edulis treated at 20ng/l TBTCl, and with an increased proportion of inactive stages in oysters treated with 2000 ng/l TBTCl. The presence and persistence of environmental pollutants, such as TBT, could represent an additional threat to the declining O. edulis populations and related taxa around the world, by increasing mortality, changing reproductive maturation, and disrupting metabolism. Our findings identify the need to consider additional factors (e.g. legacy pollution) when identifying coastal locations for shellfish restoration.

Physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) cultivars submitted to drought stress

Barley (Hordeum vulgare L.) is the second most consumed and cultivated cereal by the Moroccan population. However, it is predicted that frequent drought periods, caused by climate change, can cause problems in plant growth. Thus, the selection of drought-tolerant barley cultivars is essential to ensure the security of barley's needs. We aimed to screen drought stress tolerance in Moroccan barley cultivars. We tested the drought tolerance of nine Moroccan barley cultivars (‘Adrar’, ‘Amalou’, ‘Amira’, ‘Firdaws’, ‘Laanaceur’, ‘Massine’, ‘Oussama’, ‘Taffa’, and ‘Tamellalt’) based on physiological and biochemical parameters. Drought stress was applied by maintaining field capacity at 40% (90% for the control), and plants were randomly arranged in a greenhouse at 25 °C under natural light conditions. Drought stress decreased relative water content (RWC), shoot dry weight (SDW), and chlorophyll content (SPAD index), but significantly increased electrolyte leakage, hydrogen peroxide, malondialdehyde (MDA), water-soluble carbohydrates, and soluble protein contents, as well as catalase (CAT) and ascorbate peroxidase (APX) activities. High levels of SDW, RWC, CAT, and APX activities were recorded in ‘Firdaws’, ‘Laanaceur’, ‘Massine’, ‘Taffa’, and ‘Oussama’, which can be interpreted by high drought tolerance. On the other hand, ‘Adrar’, ‘Amalou’, ‘Amira’, and ‘Tamellalt’ showed higher values of MDA and H2O2 content, which can be linked with drought sensitivity. Physiological and biochemical parameter changes are discussed in terms of barley's tolerance to drought. Tolerant cultivars could be a good background for barley breeding in areas known for the alternative of long dry spells.

Profiling of phytochemicals using LC-ESI-MS2 , in vitro, in vivo characterization and cosmeceutical effects of Alpinia galanga (wild) extract loaded emulgel.

The potential as a depigmenting agent, sun protection, and healthy benefits is indicated by the sun protection factor, radical scavenging, and tyrosinase inhibitory activities of Alpinia galanga (wild). A stable emulgel containing A .galanga (wild) extract is prepared. This emulgel is then characterized by in vitro evaluation and identification of contents by LC-ESI-MS2 . In vivo performance is counted in terms of moisturizing, melanin level, erythema, sebum, skin fine pores and large pores analysis, and other related physiological skin parameters. DPPH radical scavenging activity, total phenolic and flavonoid counts were used to measure the free radical scavenging and tyrosinase inhibitory capability of A .galanga (wild) extract, respectively. LC-ESI-MS2 used for phytochemical analysis. Emulgels synthesize, and their globule size, Ultracentrifugation, pH, and conductivity were all evaluated. Among the developed formulations, the optimal emulgels formulation underwent 90-day stability tests for organoleptic characteristics and rheology at 8°C, 25°C, 40°C, and 40°C + 75% RH (relative humidity). Using sebumeter®, mexameter®, and corneometer®, changes in skin physiological parameters were assessed over the course of 12 weeks in 13 healthy male, Asian volunteers. VisioFace® is used for computational analysis of high-resolution pictures to determine the % area, fine pore counts, and large pore counts of the skin. The antioxidant, tyrosinase inhibitory potential and counts of total phenolic and flavonoids of A .galanga (wild) extract were impressive (85%, 75%, and 48.0mg GAE/g and 14.37 mg quercetin/g, respectively). In terms of stability evaluation, globule size (0.7528 ± 0.192μm). Optimized A .galanga (wild) ethanol aqueous (AGEA) extract loaded emulgel was stable in terms of organoleptic and in vitro evaluation. The AGEA formulation significantly reduced the amount of sebum, erythema, fine pore counts, large pore counts, fine pore % area and large pores area percentage while significantly improved the moisture and elasticity of the skin. A stable A .galanga (wild) extract loaded emulgel was successfully produced that improved the skin physiological parameters in terms of skin's sebum, erythema, moisturizing, melanin, and pores.

Low-level cadmium exposure induced hormesis in peppermint young plant by constantly activating antioxidant activity based on physiological and transcriptomic analyses.

As one of the most toxic environmental pollutants, cadmium (Cd) has lastingly been considered to have negative influences on plant growth and productivity. Recently, increasing studies have shown that low level of Cd exposure could induce hormetic effect which benefits to plants. However, the underlying mechanisms of Cd-triggered hormesis are poorly understood. In this study, we found that Cd stress treatment showed a hormetic effect on peppermint and Cd treatment with 1.6 mg L-1 concertation manifested best stimulative effects. To explore the hormesis mechanisms of Cd treatment, comparative transcriptome analysis of peppermint young plants under low (1.6 mg L-1) and high (6.5 mg L-1) level of Cd exposure at 0h, 24h and 72h were conducted. Twelve of differentially expressed genes (DEGs) were selected for qRT-PCR validation, and the expression results confirmed the credibility of transcriptome data. KEGG analysis of DEGs showed that the phenylpropanoid biosynthesis and photosynthesis were important under both low and high level of Cd treatments. Interestingly, GO and KEGG analysis of 99 DEGs specifically induced by low level of Cd treatment at 72h indicated that these DEGs were mainly involved in the pathway of phenylpropanoid biosynthesis and their functions were associated with antioxidant activity. The expression pattern of those genes in the phenylpropanoid biosynthesis pathway and encoding antioxidant enzymes during 72h of Cd exposure showed that low level of Cd treatment induced a continuation in the upward trend but high level of Cd treatment caused an inverted V-shape. The changes of physiological parameters during Cd exposure were highly consistent with gene expression pattern. These results strongly demonstrate that low level of Cd exposure constantly enhanced antioxidant activity of peppermint to avoid oxidative damages caused by Cd ion, while high level of Cd stress just induced a temporary increase in antioxidant activity which was insufficient to cope with lasting Cd toxicity. Overall, the results presented in this study shed a light on the underlying mechanisms of the Cd-mediated hormesis in plant. Moreover, our study provided a safe method for the efficient utilization of mild Cd-contaminated soil as peppermint is an important cash plant.

Single-center experience of ex vivo lung perfusion and subsequent lung transplantation.

The safety of lung transplantation using ex vivo lung perfusion (EVLP) has been confirmed in multiple clinical studies; however, limited evidence is currently available regarding the potential effects of EVLP on posttransplant graft complications and survival with mid- to long-term follow-up. In this study, we reviewed our institutional data to better understand the impact of EVLP. Lungs placed on EVLP from 2014 through 2020 and transplant outcomes were retrospectively analyzed. Data were compared between lungs transplanted and declined after EVLP, between patients with severe primary graft dysfunction (PGD3) and no PGD3 after EVLP, and between matched patients with lungs transplanted with and without EVLP. In total, 98 EVLP cases were performed. Changes in metabolic indicators during EVLP were correlated with graft quality and transplantability, but not changes in physiological parameters. Among 58 transplanted lungs after EVLP, PGD3 at 72h occurred in 36.9% and was associated with preservation time, mechanical support prior to transplant, and intraoperative transfusion volume. Compared with patients without EVLP, patients who received lungs screened with EVLP had a higher incidence of PGD3 and longer ICU and hospital stays. Lung grafts placed on EVLP exhibited a significantly higher chance of developing airway anastomotic ischemic injury by 30days posttransplant. Acute and chronic graft rejection, pulmonary function, and posttransplant survival were not different between patients with lungs screened on EVLP versus lungs with no EVLP. EVLP use is associated with an increase of early posttransplant adverse events, but graft functional outcomes and patient survival are preserved.

Transport Stress Induces Oxidative Stress and Immune Response in Juvenile Largemouth Bass (Micropterus salmoides): Analysis of Oxidative and Immunological Parameters and the Gut Microbiome.

Transport is essential in cross-regional culturing of juvenile fish. Largemouth bass (Micropterus salmoides) often exhibit decreased vitality and are susceptible to disease after transportation. To study the effects of transport stress on juvenile largemouth bass, juveniles (average length: 8.42 ± 0.44 cm, average weight 10.26 ± 0.32 g) were subjected to a 12 h simulated transport, then subsequently, allowed to recover for 5 d. Liver and intestinal tissues were collected at 0, 6 and 12 h after transport stress and after 5 d of recovery. Oxidative and immunological parameters and the gut microbiome were analyzed. Hepatocytic vacuolization and shortened intestinal villi in the bass indicated liver and intestinal damage due to transport stress. Superoxide dismutase, lysozyme and complement C3 activities were significantly increased during transport stress (p &lt; 0.05), indicating that transport stress resulted in oxidative stress and altered innate immune responses in the bass. With the transport stress, the malondialdehyde content first increased, then significantly decreased (p &lt; 0.05) and showed an increasing trend in the recovery group. 16S rDNA analysis revealed that transport stress strongly affected the gut microbial compositions, mainly among Proteobacteria, Firmicutes, Cyanobacteria and Spirochaetes. The Proteobacteria abundance increased significantly after transport. The Kyoto Encyclopedia of Genes and Genomes functional analysis revealed that most gut microbes played roles in membrane transport, cell replication and repair. Correlation analyses demonstrated that the dominant genera varied significantly and participated in the measured physiological parameter changes. With 5 days of recovery after 12 h of transport stress, the physiological parameters and gut microbiome differed significantly between the experimental and control groups. These results provide a reference and basis for studying transport-stress-induced oxidative and immune mechanisms in juvenile largemouth bass to help optimize juvenile largemouth bass transportation.

Targeted temperature management and PbtO2 in traumatic brain injury

IntroductionTargeted Temperature Management (TTM) to normothermia is widely used in traumatic brain injury (TBI). We investigated the effects to of TTM to normothermia patients with TBI (GCS≤12) monitored with multimodality monitoring, to better understand the physiological consequences of this intervention. Research questionIn TBI patients cooled to normothermia and in which brain oxygenation deteriorates, are there changes in physiological parameters which are pertinent to brain oxygenation? Material and method102 TBI patients with continuous recordings of intracranial pressure (ICP) and brain oxygen tension (PbtO2) were studied retrospectively. Non-continuous arterial carbon dioxide (PaCO2) and oxygen (PaO2) tensions, and core body temperature (Tc) were added. PaO2 and PaCO2 were also corrected for Tc. Transitions from elevated Tc to normothermia were identified in 39 patients. The 8 h pre and post the transition to normothermia were compared. Data is given as median [IQR] or mean (SD). ResultsOverall, normothermia reduced ICP (12 [9–18] −11 [8–17] mmHg, p < 0.009) and Tcore (38.3 [0.3]-36.9 [0.4] oC, p < 0.001), but not PbtO2 (23.3 [16.6]-24.4 [17.2–28.7] mmHg, NS). Normothermia was associated with a fall in PbtO2 in 18 patients (24.5 [9.3] −20.8 [7.6] mmHg). Only in those with a fall in PbtO2 with cooling did ICP (15 [10.8–18.5] −12 [7.8–17.3] mmHg, p = 0.002), and temperature corrected PaCO2 (5.3 [0.5]- 4.9 [0.8] kPa, p = 0.001) decrease. Discussion and conclusionA reduction in PbtO2 was only present in the subgroup of patients with a fall in temperature corrected PaCO2 with cooling. This suggests that even modest temperature changes could result in occult hyperventilation in some patients. pH stat correction of ventilation may be an important factor to consider in future TTM protocols.

ОТВЕТНЫЕ ФИЗИОЛОГИЧЕСКИЕ РЕАКЦИИ ОРГАНИЗМА НА УСЛОВИЯ РАБОТЫ В ЗАЩИТНЫХ ПНЕВМОКОСТЮМАХ В ВИРУСОЛОГИЧЕСКИХ ЛАБОРАТОРИЯХ

Introduction. The use of personal protective equipment when performing work operations with especially hazardous materials largely determines the level of human performance and, finally, his activity safety. The microclimate of working areas in combination with the workload can have an unfavorable effect not only on the well-being of an employee in the process of performing important work operations, but can also result in cases of temporary disability due to illness. Aim. Physiological characteristics of the functional state of the body of women – employees of virological laboratories who used protective pneumatic suits for 2–3 years preceding this study. Materials and methods. Physiological studies were carried out in a thermal chamber, where labor loads and hygienic parameters of working areas were simulated, as well as the modes of operation of protective pneumatic suits by female virologists. The results obtained are compared with the data of studies in which the individuals of the control group took part as subjects. Results. An analysis of the results of physiological studies indicates a systematic overheating of the body of the subjects in the process of performing labor operations in protective pneumatic suits. A number of characteristic changes in physiological parameters have been established for cases observed during human adaptation to heat. Conclusion. The data obtained indicate that the pneumatic suit, in the design of which the convective method of normalizing the human temperature homeostasis is realized, requires the development of special operating modes for the implementation of continuous technological or laboratory operations in it. A set of methods for evaluating the performance of personal protective equipment in terms of the thermal state of the human body was tested, which makes it possible to determine the degree of influence of environmental factors and the labor process on the functional state of employees in order to predict it and establish the optimal mode of work.

Artificial intelligence for non-invasive glycaemic-events detection via ECG in a paediatric population: study protocol

PurposePaediatric Type 1 Diabetes (T1D) patients are at greater risk for developing severe hypo and hyperglycaemic events due to poor glycaemic control. To reduce the risk of adverse events, patients need to achieve the best possible glycaemic management through frequent blood glucose monitoring with finger prick or Continuous Glucose Monitoring (CGM) systems. However, several non-invasive techniques have been proposed aiming at exploiting changes in physiological parameters based on glucose levels. The overall objective of this study is to validate an artificial intelligence (AI) based algorithm to detect glycaemic events using ECG signals collected through non-invasive device.MethodsThis study will enrol T1D paediatric participants who already use CGM. Participants will wear an additional non-invasive wearable device for recording physiological data and respiratory rate. Glycaemic measurements driven through ECG variables are the main outcomes. Data collected will be used to design, develop and validate the personalised and generalized classifiers based on a deep learning (DL) AI algorithm, able to automatically detect hypoglycaemic events by using few ECG heartbeats recorded with wearable devices.ResultsData collection is expected to be completed approximately by June 2023. It is expected that sufficient data will be collected to develop and validate the AI algorithm.ConclusionThis is a validation study that will perform additional tests on a larger diabetes sample population to validate the previous pilot results that were based on four healthy adults, providing evidence on the reliability of the AI algorithm in detecting glycaemic events in paediatric diabetic patients in free-living conditions.Trial registrationClinicalTrials.gov identifier: NCT03936634. Registered on 11 March 2022, retrospectively registered, https://www.clinicaltrials.gov/ct2/show/NCT05278143?titles=AI+for+Glycemic+Events+Detection+Via+ECG+in+a+Pediatric+Population&draw=2&rank=1.

EFEITO IMEDIATO DE UM DISPOSITIVO ORTOSTÁTICO EM AMPUTADOS PRATICANTES DE ATIVIDADE FÍSICA

ABSTRACT Introduction: Lower limb amputation affects mainly economically active young adults, causing great socio-economic impact due to impaired work capacity, socialization, and quality of life. Thus, the use of orthostatic devices could make reintegration of the individual back into the work environment possible. Objective: To evaluate the immediate effect of using a postural elevation device on physiological parameters and plantar pressure levels in amputees who practice physical activity. Methods: Sociodemographic and perceived sleep quality data were collected from 14 adults of both sexes with lower limb amputations, who practice physical activity. The participants were placed in postural elevation equipment for a period of 90 minutes with monitoring of physiological parameters such as blood pressure, heart rate, and oxygen saturation. Parameters related to the autonomic nervous system and plantar pressure levels were also evaluated. Data were also collected during recovery at 15- and 30-minute intervals after using the device. Data analysis was performed using two-way ANOVA or a mixed-effects model with Sidak's multiple comparisons test. Results: For all cardiovascular parameters (p&gt;0.05) analyzed, the activation level of the autonomic nervous system (p&gt;0.05), and plantar pressure (p&gt;0.05), no evidence of differences was observed during use of the orthostatic device in all evaluated periods. Conclusion: The use of postural elevation equipment for 90 minutes does not generate, as an immediate effect, changes in physiological parameters and postural control in amputees who practice physical activity. Level of evidence II; Therapeutic studies – investigation of treatment results. Clinical relevance statement .