Chaetomium Globosum Research Articles

Endophyte Chaetomium Globosum CGSR-13 strain enhanced plant growth promotion and antifungal activity against pokkah boeng caused by Fusarium verticillioides in India

Endophyte Chaetomium Globosum CGSR-13 strain enhanced plant growth promotion and antifungal activity against pokkah boeng caused by Fusarium verticillioides in India

Identification of cotton non-pathogenic fungal agent isolates based on morphological and MALDI-TOF mass spectrometry method

In this study, morphological and MALDI-TOF MS identification and comparison of non-pathogenic fungal species isolated from diseased root, leaf and boll tissues of cotton plants were carried out. For this purpose, surveys were conducted in Bağlar, Sur, Çınar, Bismil, Yenişehir, Ergani, Eğil, Kayapınar and Silvan districts of Diyarbakır province where cotton production is intensive between June and September 2020 and 2021. 209 samples of plants showing typical fungal disease symptoms were collected from 75 different cotton production areas. A total of 171 fungal isolates were obtained by isolation, culture and purification procedures from diseased plant tissues in the samples. The 20 isolates that were negative in the pathogenicity test in the main host were identified and compared by morphological (traditional) and MALDI-TOF MS methods. According to the results, Aspergillus niger, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Aspergillus minisclerotigenes, Penicillium nalgıovense, Chaetomium globosum, Dichotomopilus funicola, Arthroderma gloria, Pseudogmnoacus pannorum, Tichophyton interdigitale, and Penicillium sp. Penicillium sp. were found to be intensively colonized in different parts of cotton such as leaves, bolls and roots. Again, it was determined that the most common species among the total saprophyte isolates was Aspergillus niger with a high similarity rate.

Mitigating root rot in Panax notoginseng: The synergistic effects of biochar and Chaetomium globosum YIM PH30719

Root rot is the main soilborne disease that causes the replant failure of Panax notoginseng, a famous medicinal plant in Asia. Biochar and biocontrol agents have been shown to reduce replant failure separately; however, a knowledge gap still exists regarding the effectiveness and mechanisms of their combined application on the root rot incidence of P. notoginseng. In this study, we conducted a pot experiment using a randomized block design, biochar derived from tobacco stems was applied at 0, 1, 2, and 3 % (T0, T1, T2, T3) to the soil that cultivating P. notoginseng for 10 years continuously; moreover, Chaetomium globosum YIM PH30719, a newly screened biocontrol strain, was applied at 0, 1.0×107, and 1.0×1010 cfu·mL−1 (C0, C1, C2) to the soil. Six months post-transplantation of P. notoginseng seedlings, root rot incidence, soil physicochemical properties, fungal communities, and abundance of the primary pathogen Fusarium oxysporum were investigated. The results showed that, comparing with the single application of YIM PH30719, the combined application with biochar was more efficient in reducing the root rot incidence (P<0.05). P. notoginseng experienced a root rot incidence of 65.4 % under the T0+C0 treatment, however, under T3+C2 treatment, the root rot incidence was only 5.6 % (P<0.05). The reduction in root rot incidence might be linked to changes in soil pH, organic matter, and essential nutrient contents, as well as the increased soil fungal diversity and the relative abundance (RA) of beneficial Chaetomium and Trichoderma, and the decreased RA of pathogenic Fusarium and Alternaria. Consequently, the joint use of biochar and biocontrol agents (YIM PH30719) synergistically decreased the occurrence of root rot by modifying soil physicochemical characteristics and fungal communities. Since root rot is commonly found in agriculture, the results of this study may help in controlling root rot disease in other crops.

Chemical Survey and Antifungal Efficacy of Sargassum muticum's Alkaloids and Phenolic-Rich Fraction Against Airborne Toxigenic and Nosocomial Opportunistic Molds Isolates.

The Atlantic coastline of El-Jadida, Morocco, is renowned for its plentiful algae, especially brown seaweed, which is rich in active compounds known for their antifungal properties. This valuable resource offers an exciting opportunity to tackle the numerous challenges posed by invasive fungal infections, allergies, mycotoxin-related food poisoning, and drug-resistant strains. Underscoring the urgent need to explore alternative, sustainable, and environmentally friendly antifungal agents derived from algae. This study aimed to evaluate the antifungal activity of total alkaloids and phenolic-rich fractions derived from seven species of Pheophyceae: Sargassum muticum, Sargassum vulgare, Bifurcaria bifurcata, Cystoseira tamariscifolia, Cystoseira humilis, Laminaria ochroleuca, and Fucus spiralis against four fungi: airborne toxigenic isolates of Aspergillus westerdijkiae and Chaetomium globosum as well as nosocomial opportunistic isolates of Aspergillus nidulans and Scopulariopsis brevicaulis. The study also aimed to identify the most effective alga and its specific active compounds through LC-MS and GC-MS analysis. The invasive Sargassum muticum was chosen as the most potent alga in inhibiting the growth of mycelium. For the first time, the alkaloids palmatine and jatrorrhizine, along with caulerpin, have been identified. The chloroform fraction revealed the prevalence of phenolic compounds including, phenolic acids, flavonoids, and phlorotannins. The lowest minimum inhibitory concentrations (MICs), with a maximum fungal load of 108 colony-forming unit (CFU), recorded ranged from 3.12 to 6.25μg/mL by the phenolic-rich fraction against airborne toxigenic isolates, and from 100 to 200μg/mL against nosocomial opportunistic isolates by the total alkaloids. In comparison, the positive control, ketoconazole, showed higher MICs and resistance against A. nidulans. The valorization of Sargassum muticum is proposed as a green strategy to preserve the ecological balance, combat antifungal resistance, and address public health challenges.

Structural characteristics and biological evaluation of copper coating cotton material obtained by chemical reduction method

The article describes the preparation of new cotton materials obtained by chemical deposition of copper sulfide. This two-stage process consists of chelation of copper sulfate on cellulose chains of cotton (COT → COT-CuSO4) with subsequent precipitation of copper in the form of copper sulfide (COT-CuSO4 → COT-CuS) using sodium sulfide. The obtained COT-CuS composites were characterized physicobiologically. Thus, the structural characteristic of the Cotton-CuS material was determined by microscopy analysis (SEM), Atomic Absorption Spectrometry with Flame Excitation (FAAS), specific surface area and total pore volume analysis (BET). The copper functionalized cotton fabric was subjected to microbial activity tests against colonies of Gram-negative (Escherichia coli) and Gram-positive ( Staphylococcus aureus) bacteria and Chaetomium globosum, Aspergillus niger fungal molds species. Biochemical evaluation includes hematological tests of activated partial thromboplastin time (aPTT) and prothrombin time (PT). The substantial antimicrobial properties of the newly synthesized COT-CuS material with the lack of influence on blood coagulation processes offer prospects of a potential use as applications as an antibacterial/antifungal material.

Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media.

Continuous use of synthetic fungicides has led to explosive emergence of fungicide-resistant microbes. Therefore, there are urgent needs for environmentally friendly antimicrobial agents with novel modes of action. This study investigated endolichenic fungi (ELF) as a source of antimicrobial compounds against various plant pathogens. We utilized an One Strain MAny Compounds (OSMAC) approach to enhance the chemical diversity of fourteen ELF. This involved cultivation of ELF in four growth media and subsequently assessing antimicrobial activities of culture extracts. Nearly half of the culture extracts exhibited antimicrobial activity against a Gram-positive bacterium, but showed minimal activity against Gram-negative bacteria tested. Notably, culture extracts from two ELF, Chaetomium globosum and Nodulisporium sp., demonstrated significant inhibitory effects against plant pathogenic fungi. LC-MS/MS-based metabolome profiling confirmed the presence of known bioactive compounds like cyclic dipeptides and chaetoglobosins. These findings highlight the effectiveness of combining OSMAC and metabolomics for identifying antimicrobial agents for agricultural use.

Comprehensive analysis of microbiome biodiversity in popular date palm (Phoenix dactylifera L.) fruit varieties

Due to its nutritional value and health benefits, the date palm (Phoenix dactylifera L.) is an essential dietary food crop throughout Middle Eastern and African countries. Consumers are concerned about the possible microbial contamination of dates, especially since most dates arriving in local markets are unprocessed. The absence of processing increases the possibility of microbial contamination, which raises the probability of microbial contamination. This study aims to analyze and evaluate the variability of fungal and bacterial microbiota identified in the most popular date palm fruits in Saudi Arabia. The study assessed ten date variety fruits from the most popular date palm varieties for consumption in Saudi Arabia and analyzed the microbial count. Morphological and molecular characterization and comparison of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences identified 78 fungi, including 36 distinct species across 15 fungal genera. Alternaria, Fusarium, Curvilaria, Aspergillus, and Penicillium were the most frequent genera among the ten fruit cultivars studied, according to ITS-rDNA sequence analysis. Furthermore, 36 bacterial isolates were obtained from ten date varieties studied, each with a unique colony morphology. These isolates were identified based on sequence alignment and comparison of their 16S rDNA internal spacer regions to those available in public databases. The results showed that the bacterial isolates included 15 species from five bacterial genera. The results suggested that Bacillus, Stenotrophomonas, and Brucella were the prevailing genera among the ten tested fruit varieties. Some bacterial genera, such as Brucella, Achromobacter, and Stenotrophomonas, are well-known potential human pathogens. Chaetomium globosum was also recognized as air pollution causing adverse health effects such as allergies and as the causal agent of human fungal infections among the tested date varieties; the Rashodiah type exhibited the highest fungal contamination, whereas the Sagai variety displayed the lowest fungal contamination. Conversely, the Sukkari, Barhi, and Mejdool varieties were the most contaminated with bacteria among the ten tested varieties, while the Khalas variety showed the least bacterial contamination. To the best of the authors' knowledge, this study provides the initial comprehensive account of the molecular and morphological identification of all fungal and bacterial genera associated with date palm (P. dactylifera) fruits.

Improving textile waste biodegradation through fungal inoculation

Textile waste currently contributes about 780,000 tonnes of waste to landfill in Australia alone. Within landfill, biodegradable textiles take multiple years to decompose and most synthetic textiles are effectively not biodegradable. Blends of these fiber types are currently too costly to separate and recycle. Decomposition depends on the presence of suitable microorganisms already present in the landfill. This study aimed to investigate which fungi could best be used to pre-inoculate textile waste entering landfill (to reduce volume) or in a composting system (to recover non-biodegradable materials for re-use). Ten textiles were inoculated with 14 fungi well known for their degradative ability and incubated at 25–30°C for 1–2 months. Greatest weight losses (100 %) were achieved by Chaetomium globosum (Ascomycota), Trametes versicolor and Ganoderma species (Basidiomycota). In textiles with mixtures of cellulose with polyester or elastane, only the cellulose fibers were degraded and the other fiber types were left available for recycling. These results suggest that pre-inoculation with the most effective fungi could increase the rate of decay in cellulosic portions of textiles discarded into landfill, at the least reducing textile volumes quickly. Alternatively, aerobic fermentation of textile waste could recover non-biodegradable synthetic fibers for re-processing and re-use in a low-cost and environmentally benign manner, reducing the need for its manufacture de novo from petroleum derivatives.

Antibacterial and Antibiofilm Properties of Native Australian Plant Endophytes against Wound-Infecting Bacteria.

The wound management field faces significant challenges due to antimicrobial resistance (AMR) and the complexity of chronic wound care. Effective wound treatment requires antimicrobial dressings to prevent bacterial infections. However, the rise of AMR necessitates new antimicrobial agents for wound dressings, particularly for addressing bacterial pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Endophytic fungi, known for producing diverse bioactive compounds, represent a promising source of such new agents. This study tested thirty-two endophytic fungi from thirteen distinct Australian native plants for their antibacterial activity against S. aureus. Ethyl acetate (EtOAc) extracts from fungal culture filtrates exhibited inhibitory effects against both methicillin-sensitive S. aureus ATCC 25923 (MIC = 78.1 µg/mL) and MRSA M180920 (MIC = 78.1 µg/mL). DNA sequence analysis was employed for fungal identification. The most active sample, EL 19 (Chaetomium globosum), was selected for further analysis, revealing that its EtOAc extracts reduced S. aureus ATCC 25923 biofilm formation by 55% and cell viability by 57% to 68% at 12 × MIC. Furthermore, cytotoxicity studies using the brine shrimp lethality test demonstrated low cytotoxicity up to 6 × MIC (25% mortality rate) with an LC50 value of 639.1 µg/mL. Finally, the most active sample was incorporated into polycaprolactone (PCL) fiber mats via electrospinning, with resultant inhibition of S. aureus species. This research underscores the potential of endophytic fungi from Australian plants as sources of substances effective against common wound pathogens. Further exploration of the responsible compounds and their mechanisms could facilitate the development of wound dressings effective against MRSA and innovative biofilm-resistant electrospun fibers, contributing to the global efforts to combat AMR.

GC/MS analysis and evaluating of antifungal potency of algal organic extracts against environmental and clinical identified fungal strains

Marine algae are promising biologically active compounds that can be used for antimicrobial activity. The objective of this study was to evaluate the antifungal activity of seven seaweed extracts, collected from Sidi Bouzid, El Jadida (Morocco), against three fungal strains, Aspergillus steynii, Chaetomium elatum and Chaetomium globosum, responsible of numerous impacts on the environment and human health. For that, the minimal inhibitory concentration (MIC) and the chemical composition of the most active extracts were determined.The results obtained prove that methanolic extract of Osmundea pinnatifida and Ellisolandia elongata has the highest yield, followed by ethyl acetate extract of Jania rubens. Chaetomium elatum and Chaetomium globosum were the most sensitive strains using most of the organic seaweed's extract, compared to Aspergillus steynii, with 100 % of inhibition. Compared to other extracts, the ethyl acetate extract of Osmundea pinnatifida had the strongest antifungal activity with MIC of 1.95 μg/mL against Chaetomium globosum, the second strongest antifungal activity was observed using methanolic extract of Ellisolandia elongata with an MIC of 7.8 μg/mL. Chemical analysis of the active seaweeds' organic extracts shows the presence of different components dominated by heptadecanoic acid in methanolic extract of Ellisolandia elongata and methyl tetradecanoate in Osmundea pinnatifida and Bornetia secundiflora, which are known for their antibacterial and antifungal activities and may be responsible for the activity observed.

Occurrence and Importance of Chaetomium globosum as a Fungal Pathogen on Olive Leaves in Adıyaman Province of Türkiye

Occurrence and Importance of Chaetomium globosum as a Fungal Pathogen on Olive Leaves in Adıyaman Province of Türkiye

Morphological and molecular identification of fungi isolated from selected brri rice varieties

A total of 19 fungal species were isolated from the seeds of selected rice varieties (BRRI dhan 90 to BRRI dhan 99) following Tissue planting method and Blotter method. The isolated fungi were Aspergillus niger, A. ochraceus, A. oryzae, A. tamarii, A. terreus, Chaetomium globosum, Cladosporium oxysporum, Colletotrichum gloeosporioides, Corynespora cassiicola, Curvularia lunata, Curvularia soli, Daldinia eschscholtzii, Fusarium solani, Penicillium oxalicum,Penicillium sclerotiorum, Pestalotiopsis guepinii, Pyricularia oryzae and Rhizopus stolonifer. Fourteen fungi were selected for molecular identification. Out of the 19 fungal isolates, 14 were confirmed up to species level through ITS sequence based molecular analysis. Among the isolated fungi Pnicillium sclerotiorum and Curvularia soli are the new record for Bangladesh. Association of Daldinia eschscholtzii with rice seeds is also recorded first time from world. Bangladesh J. Plant Taxon. 31(1): 73-82, 2024 (June)

Identification of a new species and three new records of Chaetomiaceae associated with commercial herbal teas in northern Thailand

This study aimed to identify fungi associated with commercial herbal teas from local markets in Chiang Rai and Chiang Mai, Thailand. A new species, Arcopilus albae was isolated from mulberry leaf tea and identified based on evidence from molecular phylogenetic analysis. Phylogenetic analysis of a combined ITS, LSU, TUB2, and RPB2 dataset indicated that the new species is a distinct taxon with high statistical support. Furthermore, two isolates of Canariomyces arenarium and three isolates of Chaetomium globosum are described as new host records from five different commercial herbal teas (black and red goji berries, chrysanthemum flower, mulberry leaf, and stevia leaf). One isolate of Ch. rectangulare is described as a new host record from black goji berry and a new geographical record from northern Thailand. This study provides additional collections of fungi associated with dried commercial herbal tea products from local markets in the North of Thailand.

Enhancing soil amendment for salt stress using pretreated rice straw and cellulolytic fungi

Rice straw breakdown is sluggish, which makes agricultural waste management difficult, however pretreatment procedures and cellulolytic fungi can address this issue. Through ITS sequencing, Chaetomium globosum C1, Aspergillus sp. F2, and Ascomycota sp. SM2 were identified from diverse sources. Ascomycota sp. SM2 exhibited the highest carboxymethyl cellulase (CMCase) activity (0.86 IU/mL) and filter-paper cellulase (FPase) activity (1.054 FPU/mL), while Aspergillus sp. F2 showed the highest CMCase activity (0.185 IU/mL) after various pretreatments of rice straw. These fungi thrived across a wide pH range, with Ascomycota sp. SM2 from pH 4 to 9, Aspergillus sp. F2, and Chaetomium globosum C1 thriving in alkaline conditions (pH 9). FTIR spectroscopy revealed significant structural changes in rice straw after enzymatic hydrolysis and solid-state fermentation, indicating lignin, cellulose, and hemicellulose degradation. Soil amendments with pretreated rice straw, cow manure, biochar, and these fungi increased root growth and soil nutrient availability, even under severe salt stress (up to 9.3 dS/m). The study emphasizes the need for a better understanding of Ascomycota sp. degradation capabilities and proposes that using cellulolytic fungus and pretreatment rice straw into soil amendments could mitigate salt-related difficulties and improve nutrient availability in salty soils.

Biochemical Behavior, Influence on Cell DNA Condition, and Microbiological Properties of Wool and Wool-Copper Materials.

The paper presents the study concerning the preparation and physio-chemical and biological properties of wool-copper (WO-Cu) materials obtained by the sputter deposition of copper onto the wool fibers. The WO-Cu material was subjected to physio-chemical and biological investigations. The physio-chemical investigations included the elemental analysis of materials (C, N, O, S, and Cu), their microscopic analysis, and surface properties analysis (specific surface area and total pore volume). The biological investigations consisted of the antimicrobial activity tests of the WO-Cu materials against colonies of Gram-positive (Staphylococcus aureus) bacteria, Gram-negative (Escherichia coli) bacteria, and fungal mold species (Chaetomium globosum). Biochemical-hematological tests included the evaluation of the activated partial thromboplastin time and pro-thrombin time. The tested wool-copper demonstrated the ability to interact with the DNA in a time-dependent manner. These interactions led to the DNA's breaking and degradation. The antimicrobial and antifungal activities of the WO-Cu materials suggest a potential application as an antibacterial/antifungal material. Wool-copper materials may be also used as customized materials where the blood coagulation process could be well controlled through the appropriate copper content.

Antioxidant protection of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on H2O2-challenged HepG2 cells

Effective utilization of waste kitchen oil (WKO) is important to environmental protection and economic development. Presently, an endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide (CGP-WKO) was successfully produced through submerged fermentation with the sole carbon source of WKO. CGP-WKO had a yield of 1.53 ± 0.27 g/L and contained 95.85 % ± 3.02 % carbohydrate and 2.94 % ± 0.62 % protein. Structural feature analysis indicated that CGP-WKO contained glucose, glucosamine, mannose, rhamnose, galactose, fructose, and glucuronic acid in a molar ratio of 30.38: 1.34: 32.22: 9.68: 1.59: 0.62: 3.73. The weight-averaged molecular weight of CGP-WKO was 26.64 kDa, and its polydispersity was 1.48. Moreover, antioxidant capacity detection in vitro demonstrated that when the concentration of CGP-WKO was 1.0 mg/mL, its scavenging effects against 1,1-diphenyl-2-picrylhydrazyl; 2,2′-azinobis-di-(3-ethyl-benzothiazolin-6-sulfonic acid) diammonium salt; and hydroxyl and superoxide radicals were 45.89 % ± 1.89 %, 55.89 % ± 1.12 %, 29.02 % ± 2.61 %, and 52.76 % ± 2.12 %, respectively; and the IC50 values were 6.4612, 0.8888, 13.8585, and 0.9398 mg/mL, respectively. CGP-WKO increased the content of superoxide dismutase, catalase, and glutathione peroxidase and reduced malondialdehyde content in H2O2-challenged HepG2 cells. Overall, the present work indicated that CGP-WKO can be used as an antioxidant agent in the food, cosmetics, and pharmaceutical industries.

Marcytoglobosins A and B, Cytochalasans From a Marine Sponge Associated Chaetomium globosum 162105 Fungus.

Two new cytochalasans, marcytoglobosins A (1) and B (2) were isolated from the marine sponge associated fungus Chaetomium globosum 162105, along with six known compounds (3-8). The complete structures of two new compounds were determined based on 1D/2D NMR and HR-MS spectroscopic analyses coupled with ECD calculations. All eight isolates were evaluated for their antibacterial activity. Among them, compounds 3-8 displayed antibacterial effects against Staphylococcus epidermidis, Bacillus thuringiensis, Pseudomonas syringae pv. Actinidiae, Vibrio alginolyticus, and Edwardsiella piscicida with minimum inhibitory concentration (MIC) values ranging from 10 to 25 μg/mL.

Seasonal Variation and Distribution Patterns of Endophytic Community in Withania somnifera

Withania somnifera also known as Ashwagandha used as rasayana (tonic) in Ayurvedic system of medicine. It is widely considered as Indian Ginseng and possesses medicinal properties like adaptogenic, antistress, anxiolytic, anti-arthritic, anti-inflammatory etc. There is long history of microbes having stable symbiotic relationship with plants aiding them in growth and survival. This endophytic relationship is also been observed in medicinal plants. This study is an attempt to observe the biodiversity of endophytic fungal community in Withania somnifera and to understand their colonization. The study was aimed to isolate endophytes from different organs of Withania somnifera with seasonal variations. A total of 131 endophytic fungi were isolated from 450 explants from different organs like leaves, stems and roots of W. somnifera. The overall colonization rate of endophytes in winter, summer and monsoon were 42.67%, 7.33% and 37.33% respectively. Among the identified isolates, most abundant and frequently isolated genera were Alternaria, Fusarium and Chaetomium sps. The dominant species isolated in winter were the Fusarium moniliformae and Chaetomium globosum from leaves with 17.24% and 13.79% dominant frequency, respectively. While Alternaria raphani and Chaetomium globosum were most dominant species in summer from leaves with 40% dominant density. In the monsoon, Microsporum ferruginem was the most dominant species isolated from leaves and stem with 61.29% and 42.86%, respectively. The most recurring endophytic species of Ascomycetes group were Alternaria alternata and Chaetomium globosum seen in all seasons.

Novel insights into dimethylsulfoniopropionate cleavage by deep subseafloor fungi

Dimethylsulfoniopropionate (DMSP), a key organic sulfur compound in marine and subseafloor sediments, is degraded by phytoplankton and bacteria, resulting in the release of the climate-active volatile gas dimethylsulfide (DMS). However, it remains unclear if dominant eukaryotic fungi in subseafloor sediments possess specific abilities and metabolic mechanisms for DMSP degradation and DMS formation. Our study provides the first evidence that fungi from coal-bearing sediments ∼2 km below the seafloor, such as Aspergillus spp., Chaetomium globosum, Cladosporium sphaerospermum, and Penicillium funiculosum, can degrade DMSP and produce DMS. In Aspergillus sydowii 29R-4-F02, which exhibited the highest DMSP-dependent DMS production rate (16.95 pmol/μg protein/min), two DMSP lyase genes, dddP and dddW, were identified. Remarkably, the dddW gene, previously observed only in bacteria, was found to be crucial for fungal DMSP cleavage. These findings not only extend the list of fungi capable of degrading DMSP, but also enhance our understanding of DMSP lyase diversity and the role of fungi in DMSP decomposition in subseafloor sedimentary ecosystems.

Marchaetoglobins A-D: Four Cytochalasans with Proangiogenic Activity from the Marine-Sponge-Associated Fungus Chaetomium globosum 162105.

Four new cytochalasans, marchaetoglobins A-D (1-4), along with five known compounds (5-9), were isolated from the marine-sponge-associated fungus Chaetomium globosum 162105. Compounds 1-4 represent examples of 19,20-seco-chaetoglobosins, of which compound 1 is the first furan-containing cytochalasan. Their structures and absolute configurations were elucidated by extensive spectroscopic analyses and electronic circular dichroism calculations. Compounds 5, 8, and 9 displayed weak to moderate antibacterial activities against Bacillus thuringiensis, Edwardsiella piscicida, Vibrio alginolyticus, and Pseudomonas syringae pv. actinidiae with minimum inhibitory concentration values ranging from 5 to 25 μg/mL. In addition, compounds 2, 3, and 5 showed potent in vivo proangiogenic activity in transgenic zebrafish, comparable to the positive control.