In the last two decades, many works on vesicle formation from natural amphiphiles (mainly phospholipids) and synthetic surfactants were reported [1,2]. However, up to 10 years ago amphiphiles used to form vesicles are mainly the double chained compounds including natural and synthesized amphiphiles. In 1989, Kaler et al. [3] revealed the vesicle formation from mixed cationic and anionic surfactants using cetyltrimethyl-ammonium tosylate and sodium dodecylbezene sulfonate. Similar work was done in our laboratory, using mixed surfactants of carboxylate and alkyltrimethylammonium compounds [4,5]. However, most works on vesicle formation have been involved in aqueous systems and those in non aqueous systems are less. Some works about vesicle formation in aprotic solvent systems were reported [6,7], which were concentrated on the systems of double-chained fluorocarbon surfactants [8–11]. On the other hand, the studies about vesicle formation in non aqueous polar solvent and mixed polar solvents’ systems are mainly on the liposome of phospholipids [12– 14]. Comparing with phospholipids and other double-chained amphiphiles, the cationic and anionic surfactants used as precursors for vesicle formation have the predominances of simple structure, excellent stability, and convenience in production. Therefore, investigation on vesicle formation by cationic and anionic surfactants is of great significance in both theoretics and applications. It is well known that many drugs are insoluble in water but soluble in organic solvents, and many useful reactions can be carried out in nonaqueous systems. However, the investigations about vesicle formation of mixed cationic and anionic surfactants in non-aqueous polar solvents are scanty. Obviously, it is important to study the vesicle formation in non aqueous or mixed solvents. In a previous paper [15], we reported the * Corresponding author.