Human papillomavirus (HPV) have been recognized as oncogenic factor in cervical carcinoma. There was an increased incidence of cervical cancer in Chinese women. However, little is known about the prevalence of HPV genotypes in Chinese women with cervical cancer and risk factors of cervical cancer. Cervical exfoliated cells were prospectively collected from 792 women with cervical cancer referred for colposcopic examination. The individual HPV genotypes in these specimens were identified (HPV GenoArray test kit; Hybribio Ltd., Hong Kong). Meanwhile, the clinical data and laboratory result of these patients were gathered. HPV-positive was found in 588/792 (74.2%). The prevalence of the HR-HPV were 516/792 (65.2%). and 51.0% of these patients were infected with mixtures of HR genotypes. HPV positive rate in various clinical stages and differentiation degree of uterine cervical cancer had no statistical differences (P>0.05). HPV positive rate in squamous cancer and adeno-carcinoma had statistical differences respectively with other pathological types (P<0.05).The logistic regression analysis showed that HPV16 infection or HPV58 infection or age over 40 might increase the risk of carcinogennesis of the cervix. The infection rate and superinfection rate of HPV from Chinese women with disease of uterine cervix were high. The patients with HPV16 or HPV58 infection or age over 40 years were associated with increased risk of cervical cancer. Key words: Human papillomavirus, Chinese women, cervical cancer.