Cerrado Biome Research Articles

Ocular outcomes of toxoplasmosis in the Amazon-Cerrado transition zone, Brazil: a population-based study.

Eye damage represents one of the gravest outcomes of toxoplasmosis, potentially leading to varying degrees of vision impairment, up to and including blindness. Monitoring ophthalmological indicators in individuals affected by toxoplasmosis, alongside understanding its impact on visual acuity, can motivate the scientific community to advocate for strategies to prevent vision loss associated with toxoplasmosis. This is a retrospective study analysing medical records and clinical documentation from patients at an ophthalmological clinic in a city located at the intersection of the Cerrado and Amazon Forest biomes. It was noted that >20% of patients exhibited visual acuity ≤20/200, a condition medically recognized as blindness for the affected eye. Our study highlights a significant portion of individuals experiencing lasting visual impairments due to toxoplasmosis. These findings remain obscured by the existing national guidelines, which only mandate reporting for congenital and gestational toxoplasmosis cases, thereby overlooking sporadic manifestations and their consequential effects. The effort to identify ophthalmologic consequences is crucial both for personalized clinical care and for shaping national guidelines for managing toxoplasmosis.

Species limits in Wiedomys (Cricetidae: Sigmodontinae) reinforce the South American São Francisco River as a biogeographic barrier

Abstract Red-nosed mice (genus Wiedomys) are restricted to the Brazilian Cerrado and Caatinga biomes, distributed along the São Francisco River (SFR). Until recently, Wiedomys was considered monotypic, but 2 species with poorly defined geographic and morphological limits have been recognized: W. cerradensis and W. pyrrhorhinos. Our study used morphological, phylogenetic, and cytogenetic analyses to review and detail species limits within the genus and test whether the SFR could be associated with divergence between lineages. This integrative approach indicated that the SFR delineates the geographic boundaries between species, with W. pyrrhorhinos on the right bank and W. cerradensis on the left bank. The species are reciprocally monophyletic and diverge in cranial size, nasal morphology, and facial pelage color pattern. Karyotypic differences between and within W. pyrrhorhinos and W. cerradensis were also detected, and new chromosome complements (2n = 62, FN = 96 and 2n = 62, FN = 88, respectively) were found. Divergence time (0.339 to 0.025 Ma) is concordant with the timing of diversification of other co-distributed vertebrate taxa, and close to a younger estimate for the formation of SFR’s recent lower course to the east coast. Therefore, our study considerably expands knowledge of the geographic range of the genus, diagnoses of species, karyotypic variability between and within species, and reinforces the role of SFR as an important vicariant barrier in northeastern Brazil.

Mutations in the main antigenic sites of VP7 and VP8* from G3P[8] rotavirus a strains circulating in Brazil may impact immune evasion to rotavirus vaccination.

In the post-rotavirus (RVA) vaccination era, uncommon and zoonotic strains have emerged as causative agents of acute gastroenteritis in humans, including the equine-like G3P[8] strains. First identified in 2013, this strain has quickly spread worldwide, reaching the position of the most prevalent genotype in many countries, including Brazil. Here, we report full genotype characterization and phylogenetic analysis of two equine-like G3P[8] strains detected in Goiás, a state in the Cerrado biome of the Brazilian Midwestern region, during the year of 2019. The strains were detected in different socioeconomic and demographic contexts: GO-MR from an asymptomatic adult living in a rural traditional community and GO-H5 from a symptomatic child from the state capital, with access to safe drinking water and essential sanitation services. These strains also displayed different backbone constellations considering the NSP2 gene segment (G3-P [8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for GO-MR and G3-P[8]-I2-R2-C2-M2-A2-N1-T2-E2-H2 for GO-H5). Furthermore, significant mutations in the main epitope sites of the VP7 and VP8* proteins of the detected strains, and other Brazilian G3P[8] viruses, were found with the comparison to RV1 and RV5 vaccine proteins, indicating a potential ability of these viruses to evade vaccine protection, which may contribute to their prevalence both nationally and globally. In summary, this study corroborates the genetic diversity of equine-like G3P[8] DS-1-like strains circulating worldwide, highlights the epidemiological importance of adults as reservoirs of RVA and shows the substantial differences between these emerging strains and the currently used anti-RVA vaccines, which may partially explain their predominance due to potential evasion of vaccine-induced protection.

Mapping Climatic Regions of the Cerrado: General Patterns and Future Change

ABSTRACTThe Cerrado biome, renowned for its biodiversity and threatened by rapid land transformation, encompasses a vast savanna ecosystem in Brazil. The region is characterised by a seasonal climate, influenced by a myriad of meteorological systems creating diverse and non‐homogeneous rainfall regimes across the region. To account for this heterogeneity, we propose a novel classification of the Cerrado using rainfall data to delineate three distinct climatic regions: Eastern, Southern and Central‐West Cerrado. The Eastern region exhibited the driest and most seasonal climate, marked by high vapour pressure deficit (VPD) and predominantly open‐canopy vegetation. Conversely, the Southern region, characterised by lower seasonality, boasts a higher proportion of forest cover and lower mean VPD. The Central‐West region, encompassing diverse landscapes, featured areas with higher precipitation levels, particularly along the Amazônia border. Furthermore, we conducted trend analyses on observed station data and used CMIP6 models to evaluate future scenarios under differing emissions trajectories. While observed trends in mean rainfall were marginal, VPD demonstrated a notable upward trend of approximately 1% annually throughout the biome. Climate models indicated a substantial drying close to the Amazônia border, and wetter conditions in the southeast. All Cerrado regions are anticipated to experience amplified seasonality and VPD, with VPD projected to surge by approximately 30% (60%) under low (high) emissions scenarios by the end of the century. Notably, the transition from the dry to wet season was the most affected. Our study provides critical insights on how the climatic heterogeneity of the Cerrado shapes vegetation structure distribution and how future changes will exacerbate water stress throughout the biome. These findings underscore the importance of understanding climate variability for effective conservation and management strategies in the region.

Relicts of Carrasco in the Tocantins Cerrado: Diversity and Floristic Relationships

Objective: To describe the floristic and structural diversity of the Carrasco in the municipality of Porto Nacional. Theoretical Framework: This study is based on analyzing the floristic diversity, vegetation structure, and floristic similarity to verify its classification as a phytophysiognomy associated with the Cerrado of Tocantins. Method: Fifty plots measuring 10 x 10 meters were allocated. Sampling criteria included circumference ≥ 10 cm at ground level and height ≥ 1 m. The phytosociological data were analyzed using the FITOPAC software. Results and Discussion: A total of 4,110 individuals were found, distributed among 59 species from 37 genera and 22 botanical families. The vegetation showed homogeneity and diversity comparable to other Cerrado areas but with distinct characteristics, such as the presence of lianas and low ecological dominance. The floristic similarity analysis revealed a low similarity between the "Carrasco" and other Cerrado phytophysiognomies, highlighting the uniqueness of this vegetation. Research Implications: The 'Carrasco' possesses unique floristic, structural, and similarity characteristics, being a vegetational relict that justifies its designation as a new phytophysiognomy of Tocantins. Originality/Value: This study contributes to the literature by providing a detailed analysis of the floristic composition of the “Carrasco” phytophysiognomy, a new xerophytic and deciduous phytophysiognomy on sandy soils in the state of Tocantins, highlighting its importance for biodiversity conservation within the Cerrado biome. The relevance of this research is to expand the understanding of the ecological characteristics of this relic, offering essential insights for future conservation and management actions.

Radiocarbon dating and isotopic paleoecology of Notiomastodon platensis (Mammalia: Proboscidea) from the late Pleistocene of Minas Gerais, Brazil

Proboscidea is a group of large mammals abundant in the fossil record and in Brazil it is represented by Notiomastodon platensis (Ameghino, 1888). To improve the understanding on its taxonomy, chronology, annual diet, and habitats paleoenvironmental aspects, we conducted a morphological description, absolute dating, and analysis of stable isotopes of carbon and oxygen (δ13C and δ18O) in Notiomastodon' remains from the northern (Norte de Minas) and western (Triângulo Mineiro) mesoregions of Minas Gerais State, Brazil. The material includes isolated teeth, mandibular portions, and postcranial bones at different levels of fragmentation, associated with at least eight adults and one juvenile. The only individual from Triângulo, found in Campina Verde municipality, was dated at 27,715–27,903 Cal yr BP. It presented a mixed diet based on C4 plants (piC4 = 90 %; δ13C = 0.5 ‰), which implies the occupation of an open environment, mostly pasture. The region was relatively humid (δ18O = 24.7 ‰), which is supported by the existence of humidity corridors in the Amazon region. A similar phytophysiognomy was inferred for the Norte de Minas region between at least 21,966–22,279 Cal yr BP and 18,944–19,157 Cal yr BP. However, one specimen showed a different δ13C value (piC3 = 0.87 %; δ13C = −10.2 ‰). This indicates it lived in a transitional environment between low-density forest and arboreal savannah. This likely occurred during a period that favored the expansion of trees and shrubs. The region underwent climate change, from relatively humid conditions (δ18O = 25.6 ± 0.2 ‰) to expressively dry (δ18O = 34.6 ‰) between the dated periods, a change corroborated by some palynological data. The multiannual paleoecological analysis, based on sequential sampling of three dentin layers of an incisor, indicated the relative stability of vegetation and climate despite fluctuations in hydrology. The perceived disassociation between vegetation dynamics and local hydrology corroborates the idea that factors other than precipitation may play a significant role in the environmental dynamics of the Cerrado biome.

Exploiting Legal Reserve Compensation as a Mechanism for Unlawful Deforestation in the Brazilian Cerrado Biome, 2012–2022

This research investigates the illegal Vegetation Suppression Authorization (VSA) for Legal Reserve Compensation (LRC) in Tocantins, Brazil, between 2012 and 2014. The VSA is a license issued by state environmental agencies that authorizes the removal or clearing of native vegetation. This infraction resulted from the state environmental agency’s mistaken interpretation of Resolution 07/2005 of the State Environmental Council and the Native Vegetation Protection Law, which operates nationally and takes precedence over state resolutions and laws. The study area focuses on eight municipalities contained in the Formoso, Pium, and Javaés river basins. The methodology examines irregular VSA-LRC cases through geospatial data analysis, multitemporal analysis using remote sensing data, and economic assessments in both the origin and destination areas of the compensatory reserve. The results showed that among the 217 VSA-LRC applications during the period, 93 had corresponding geospatial data, indicating that 57.1% of the geospatial data were missing. This discrepancy between the authorized area and the available geospatial information restricts the oversight capability of the regulatory agency. A decade-long deforestation analysis of VSA-LRCs using remote sensing data considered three phases: 2012–2014 (application and issuance of VSA-LRCs), 2015–2018 (operational issuance), and 2019–2022 (post-expiration of VSA-LRCs). The results revealed a total VSA-LRC area of 343.291 km2 (34,329.10 ha), with 229.1169 km2 (22,911.69 ha) of deforestation. Notably, 54.877 km2 (5487.70 ha) of this deforestation occurred in 2019–2022, after the VSA-LRC had expired. The analysis of the LRC origin and destination areas demonstrates a unidirectional strategy, from economically more valuable areas to less valuable ones, according to land market dynamics. Therefore, this investigation concludes the need for greater administrative transparency, the development of a reliable geospatial data system for monitoring, and policy changes and actions to enhance environmental protection.

Evaluation of chemical constituents in Norantea guianensis aubl. Extracts, embryotoxicity, and acetylcholinesterase inhibitory potential in Danio rerio.

Norantea guianensis Aubl., commonly referred to as the parrot flower or macaw-tail vine, is a plant species found in the Brazilian Amazon, Caatinga, and Cerrado biomes. It is used in folk medicine for its anxiolytic properties, as well as its ability to alleviate headaches and reduce fever. Nevertheless, despite its therapeutic benefits, the impact on embryonic development has yet to be fully explained. The objective of this study was to evaluate chemical constituents by HPLC-DAD, UV-visible and classical phytochemistry and the LC50 of ethanolic extracts from the stem and leaves of N. guianensis in Danio rerio after 96h and to investigate their effect on developmental morphology. The findings were then related to both Acetylcholinesterase (AChE) activity and the plant's chemical composition. Zebrafish embryos were exposed to 0, 20, 40, 60, 80 and 100mg/L concentrations of stem and leaves extracts. Phytochemical analysis revealed that the stem extract contained predominantly phenolic compounds, tannins, and anthraquinones, while the leaf extract contained alkaloids and flavonoids. The LC50 values for the stem and leaf extracts were 64.55mg/L and 7.16mg/L, respectively, being the stem extract was more toxic than the leaf extract. Induced malformations and alterations in the zebrafish development in different concentrations for both extract including pericardial edema, increased heart rate, spinal malformation and equilibrium disruption. Unlike to stem extract, the leaf extract delayed larval hatching and inhibited AChE activity. The findings indicate that the leaf extract possesses higher embryotoxicity and its use should be avoided during pregnancy.

The Use of Integrated Crop–Livestock Systems as a Strategy to Improve Soil Organic Matter in the Brazilian Cerrado

This study aimed to analyze the carbon (C) stock and stabilization of soil organic matter in particulate- and mineral-associated fractions across different land use systems after 32 years of experimentation in the Brazilian Cerrado. The experiment was established in 1991 and was performed in Planaltina-DF. The treatments evaluated included continuous pasture with monoculture grasses; integrated crop–livestock systems under no tillage; continuous cropping under no tillage; minimum tillage; and the preservation of the native Cerrado biome in its original condition. Soil sampling was performed to a depth of 30 cm. Carbon and nitrogen (N) stocks were quantified for the years 2001, 2009, 2013, and 2023, with soil organic matter fractionation performed on samples from 2023. Land use change resulted in significant losses of soil C and N in areas managed with conventional soil preparation practices. Systems that promote plant diversity, such as integrated crop–livestock systems, enhanced soil C and N stocks (72.8 and 5.5 Mg ha−1, respectively) and increased both particulate organic matter and mineral-associated fractions, most of which were in more stabilized forms. Integrated crop–livestock systems are management practices that offer an effective alternative to present methods in terms of combating climate change and supporting ecosystem sustainability.

The Ecological Modernization Paradigm applied to fiduciary donations to traditional peoples organizations in the Brazilian Cerrado Biome

This article aims to understand the modus operandi used by the Center for Alternative Agriculture in Northern Minas Gerais (CAA), an non-governmental organization of traditional peoples from the northern region of the state of Minas Gerais, in the course of the implementing the Dedicated Grant Mechanism for Indigenous Peoples, Quilombolas and Traditional Communities of the Brazilian Cerrado Biome (DGM Brazil Project). Based on an empirical study carried out while I was an employee of a non-governmental organization, I propose a differentiated theoretical-methodological format for interdisciplinary research, combining professional experience, documentary analysis and bibliographic review. The article is concerned with linking this experience to other economic and political contexts, with emphasis on the recent dynamics of management and implementation of fiduciary donations by non-governmental organizations in Latin America. It supports the hypothesis that these experiences reproduce the Ecological Modernization Paradigm, based in the belief in efficiency as a means of achieving sustainable development.

Performance of infiltration trenches in different soils in native vegetation and urban area of the Federal District of Brazil

The increase in flooding events in major cities has driven the search for new strategies that incorporate Low Impact Development (LID) units into urban drainage. This research analyzes the performance of infiltration trenches to determine flow and infiltration physical parameters in different soil types in the Federal District of Brazil (FDB), comparing urban area and preserved Cerrado biome. The results show that most soils in the Federal District of Brazil have good infiltration conditions, particularly the Red Latosol and Red-Yellow Latosol. Infiltration trenches were effective in storing significant volumes of water, especially during initial runoff events where favorable soil moisture conditions exist. The Regolithic Neosol exhibited low infiltrability, making its use for infiltration trenches not recommended. Keywords: cerrado, infiltration trench, red latosol.

The First Standardised Sampling Plan Designed to Scout Dalbulus maidis (Hemiptera: Cicadellidae) Adults in Corn Crops Using Yellow Sticky Traps

ABSTRACTThe corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae) is one of the main pests in corn crops causing yield reductions of up to 70%. Dalbulus maidis primarily damages corn by transmitting pathogens. Direct plant damage is caused by sap suction and toxin injection. Sampling corn fields to determine the D. maidis density for decision‐making systems can be challenging due to field size, time and cost. Yellow sticky traps (YST) are commonly employed to determine the presence and density of D. maidis because they are easy to use and allow quick and low‐cost assessment. This study aimed to propose and evaluate a new sampling plan for D. maidis in corn crops using YST. This research was carried out in commercial corn crops in the Brazilian Atlantic Forest and Cerrado biomes for 3 years. The pest density in traps and on plants were moderately (r = 0.54–0.66) and significantly (p < 0.0001) correlated. The density evaluated in the traps showed relative variance of less than 25% and sampling time of up to 2 min per sample unit. The negative binomial distribution was adequate to represent the D. maidis density probability distribution for the YST sampling method with K common (2.8979) among all the fields evaluated. The sampling plan consisted of installing one trap every two hectares. The plan's total cost ranged from US$/ha 0.41 to 0.50, with a total sampling time of up to 2.5 min/ha. We propose this new sampling plan for D. maidis, which is suitable for incorporation into management programmes in corn crops, as it is representative, accurate, fast and low cost.

Evapotranspiration measurements in pasture, crops, and native Brazilian Cerrado based on UAV-borne multispectral sensor.

In Brazil, agriculture consumes most of the available freshwater, especially in the Cerrado biome, where the rain cycle is marked by long periods of drought. This study, conducted at the Brazilian Agricultural Research Corporation (Embrapa) Research Corporation unit in Santo Antônio de Goiás, Goiás, Brazil, estimated evapotranspiration (ET) in different crops and soil cover. Using multispectral unmanned aerial vehicle (UAV) images, Sentinel satellite data, weather station information, and towers employing the eddy covariance method, we applied the "Simple Algorithm for Evapotranspiration Retrieving" (SAFER) to calculate ET in common bean, pasture, and semideciduous seasonal forest areas. The results showed a good agreement between UAV and satellite data, with R2 = 0.84, also validated with flow towers by the eddy covariance method. UAV-based ET was observed to correspond well to tower (EC) during full vegetative development of beans but is underestimated at the beginning of planting and in the final periods of plant senescence, due to the influence of soil or straw cover. These findings contribute to a better understanding of water dynamics in the system and to enhancing sustainable agricultural practices. This method, adapted for multispectral aerial imaging, can be applied flexibly and on-demand, in different contexts and ground cover. The study highlights the importance of integrated agricultural practices for better management of water resources and preservation of the Cerrado in balance with cultivation areas.

Harvestmen (Arachnida: Opiliones) from the Atlantic Forest-Cerrado transition zone of Luminárias, southern Minas Gerais state, Brazil

The Cerrado and Atlantic Forest biomes are global biodiversity hotspots. However, these environments still face large knowledge gaps concerning fauna inventory studies, such as those on harvestmen. Our study aimed to sample harvestmen species in a transition area between the Atlantic Forest and Cerrado, contributing to future biodiversity assessments and conservation efforts in Brazil. The surveys were conducted in the municipality of Luminárias, southern Minas Gerais state, between May 2023 and March 2024. We collected 185 individuals from six species and three morphospecies with a wide geographical distribution, but whose conservation status has not been assessed, which may affect their protection. Additionally, loss of native vegetation may lead to reductions in their populations. Our study supports current literature, which suggests the need for more inventories and the creation of a Conservation Unit in the Luminárias region.

Distinct morphophysiological responses of the native C4 grasses Axonopus siccus and Eragrostis polytricha to shading

Light, an important abiotic factor that shapes plant growth, is explored here in its impact on two native C4 grasses of campo rupestre, an important and unique montane ecoregion in Brazil that occurs across the biomes Cerrado, Atlantic Forest and Caatinga. Axonopus siccus and Eragrostis polytricha seedlings were subjected to two light conditions: (i) artificial shading and (ii) full sun. The evaluations included growth, nutrition, photosynthesis, leaf anatomy, and metabolism. Shading influenced most of the growth parameters of E. polytricha but left A. siccus morphology almost unchanged. The parameters of leaf anatomy, nutrient content, and chlorophyll a fluorescence exhibited consistent patterns between species. In particular, A. siccus showed higher Amax and light compensation point in shaded conditions, while E. polytricha revealed distinctive changes in carbon metabolism (soluble sugars, sucrose, and starch content), explaining its increased shade growth. These findings underscore various light stress responses in these native C4 grasses.

Exploring Natural Infection in Alternative Hosts of Corn Stunt Spiroplasma and Tracking the Temporal Dynamics of Corn Stunt Disease in the Brazilian Cerrado Biome

The corn stunt disease complex, caused by the mollicutes Spiroplasma kunkelii (Css) and the maize bushy stunt (MBS) phytoplasma, is a major phytosanitary issue for corn production in the neotropical region of Brazil. In this study, we investigated the presence of alternative hosts for S. kunkelii (Css) in the Brazilian Cerrado biome and explored the potential for asymptomatic Css infections in maize plants. To identify possible alternative hosts, we collected samples from ornamental and native plants located in Cerrado regions with a high incidence of corn stunt disease. We also monitored the disease’s progression over time and calculated the area under the disease progression curve (AUDPC). Additionally, we sampled healthy, asymptomatic maize plants growing near symptomatic ones and analyzed all the samples using qPCR to detect the pathogen. Our results showed no Css infection in the sampled alternative host species and no asymptomatic infections in the nearby maize plants. The incidence of maize stunting varied significantly among the sample years and counties. In Aparecida do Rio Negro, the infection rate was six times higher in 2020 compared to 2019, while Porto Nacional showed an 11-fold increase. During surveillance from March to July, the infection rate at the Sierra site went from less than 3% in March 2019 to 6% in July (F1,4 = 415.84; p = 0.0003). MBS infection increased significantly, while S. kunkelii remained stable below 3%. In 2020, MBS increased from 3% to more than 12%. A similar trend was observed at the Sede site, where MBS increased from 9% in 2019 to 11% in 2020. An increase in the AUDPC was observed both within individual years and between 2019 and 2020, indicating a worsening trend in disease severity. Overall, our findings reinforce the need for preventive measures in managing maize crop fields.

Therapeutic applications of Eugenia klotzschiana O.Berg: A Systematic Bibliographic Review

Eugenia klotzschiana O.Berg is an endemic plant from the Brazilian Cerrado biome with substantial chemical and genomic diversity. This systematic review aimed to identify the therapeutic applications of the plant in the SciELO, Bireme, LILACS, PubMed/Medline, Web of Science, Google Scholar, and Science Direct bibliographic databases, using the START Program for identifying and selecting studies. Forty-six articles were obtained, of which 42 were excluded, and four were included in the review. The described therapeutic applications were antioxidant, antimicrobial, antiparasitic, and cytotoxic activities of fruits, flowers, and leaves. The small number of published articles indicates the need for developing studies on the therapeutic potential of E. klotzschiana O. Berg.

A new record of Platyphora flavozonata (Blanchard, 1843) (Chrysomelidae: Chrysomelinae) in the Parque Estadual Altamiro de Moura Pacheco, a Cerrado remnant

The Cerrado biome is a vast ecoregion of tropical savanna in eastern Brazil. The leaf beetle species Platyphora flavozonata (Blanchard, 1843) is a new record for Parque Estadual Altamiro de Moura Pacheco in the Cerrado biome. Distribution of record of the species, the high-resolution photos and notes on external morphology are provided.

Conservation gaps for Brazilian bats, limited protection across conservation units and the importance of the indigenous lands.

In Brazil, there is 13% of the world's bat diversity, is the second most diverse group of mammals, playing a crucial role in providing ecosystem services that benefit humans. However, anthropogenic disturbances exacerbate processes of species extinction, shifts in geographic distributions, and phenological changes, despite efforts to safeguard biodiversity through the creation of Conservation Units and Indigenous Lands. Moreover, gaps in taxonomic knowledge and challenges related to species distribution hinder the effective implementation of conservation strategies in protected areas. This study assesses the contribution of Brazilian Conservation Units (both Full Protection and Sustainable Use) and Indigenous Lands to the conservation of bat species and their ecosystem services. It also presents maps illustrating species richness by trophic guilds and threat classification according to IUCN, including species listed as Data Deficient. The findings reveal low percentages of potential bat distribution areas within these protected regions, especially for insectivorous, nectarivorous, and frugivorous bats in the Cerrado biome, which are classified as Near Threatened. Additionally, the highest bat species richness was observed in the Amazon and Atlantic Forest biomes.

Accessing the genetic diversity of natural populations of Acrocomia aculeata (Arecaceae) through microsatellites

Acrocomia aculeata holds cultural and economic importance as the second-largest producer of oleaginous fruits in arid and semi-arid regions, with potential for exploitation as an agricultural crop. It is a tropical arborescent species distributed throughout tropical and subtropical Americas, with a significant occurrence of natural populations in the Cerrado biome. Its broad distribution across various landscapes is supported by a mixed reproductive system, diverse pollination strategies, and resilience to environmental stressors. Despite its resilience in fragmented habitats, anthropogenic habitat fragmentation generally harms populations, affecting gene flow and genetic diversity. In this context, this study aimed to assess genetic diversity and structure of natural populations of A. aculeata from northern Minas Gerais using microsatellites. The five natural populations were named according to the municipality where they were collected, being them Espinosa, Mirabela, Claro dos Poções, Grão Mogol, and Itacambira. The results revealed that four SSR loci amplified 46 different alleles, totaling 1352 alleles. The loci showed significant deviations from Hardy-Weinberg Equilibrium, with a mean f of 0.288. Among the populations analyzed, ITA was the most genetically diverse, while ESP was the least diverse. As subpopulations, they displayed high genetic structure, with a mean FST of 0.329 and FIT of 0.484. The study suggests the natural populations of Macaw palm in northern Minas Gerais that can be used for germplasm banks, and the ones that requires human intervention to restore genetic diversity.