Event Abstract Back to Event Myelination in the pituitary adenylate cyclase activating polypeptide (PACAP) deficient mice A. Vincze1, D. Reglődi2, Z. Helyes3, H. Hashimoto4, N. Shintani4 and H. Ábrahám1 1 University of Pécs, Faculty of General Medicine, Hungary 2 University of Pécs, Department of Anatomy, Hungary 3 University of Pécs, Department of Pharmacology and Pharmacotherapy, Hungary 4 Osaka University, Graduate School of Pharmaceutical Sciences, Japan The neuroprotective effect of pituitary adenylate cyclase activating polypeptide (PACAP) has been established in several studies. Receptors for PACAP are present in many cell types including oligodendrocyte progenitor cells (OLPs), and PACAP has been shown to stimulate proliferation, delay maturation of OLPs and consequently the initiation of myelination in vitro cerebellar slice cultures. In order to elucidate the role of PACAP in myelination in vivo, we examined myelination in PACAP-deficient and wild type (WT) mice using immunohistochemistry against a major myelin protein called myelin basic protein (MBP). We found no difference in the number of MBP-immunoreactive oligodendroglial cells (OLG) at postnatal day 3 (P3) between the two groups, but at P5 more OLGs were found in the WT than in PACAP-deficient mice. The first MBP-immunoreactive fibers were seen at P8 in the corpus callosum, cingulum, and fornix in the PACAP-deficient and WT mice, but the density of myelinated fibers was lower in WT. In older animals until P60, stronger myelination was observed in all cortical and subcortical regions of the brain in PACAP-deficient than in WT mice, indicating that PACAP has an inhibitory role on myelin formation. We conclude that PACAP delays myelination in mice in vivo. Further investigations are needed to shed light on the modulatory mechanism of PACAP on myelination. Acknowledgements Supported by OTKA grants #K 047109, K72592, CNK78480, by the Bolyai Scholarship Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Vincze A, Reglődi D, Helyes Z, Hashimoto H, Shintani N and Ábrahám H (2011). Myelination in the pituitary adenylate cyclase activating polypeptide (PACAP) deficient mice. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00070 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers A. Vincze D. Reglődi Z. Helyes H. Hashimoto N. Shintani H. Ábrahám Google A. Vincze D. Reglődi Z. Helyes H. Hashimoto N. Shintani H. Ábrahám Google Scholar A. Vincze D. Reglődi Z. Helyes H. Hashimoto N. Shintani H. Ábrahám PubMed A. Vincze D. Reglődi Z. Helyes H. Hashimoto N. Shintani H. Ábrahám Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.