Three different genera of opalinids were recovered from 14 out of 24 species of anurans collected in 12 departments of Colombia. At least 7 species of the opalinid genus Zelleriella were found in 10 different frogs. Five species of the genus Opalina were present in 4 colombian frogs and one Cepedea was recovered from a toad. None of the opalinid species identified has been aescribed previously from Colorribia, and except for Bufo marinus , all host species mentioned seem to be new records for the cited opalinids.