Central-West Brazil Research Articles

Molecular detection and genetic characterization of Ehrlichia canis and Ehrlichia sp. in neotropical primates from Brazil

The Anaplasmataceae family includes obligate, arthropod-transmitted intracellular bacteria that can be zoonotic and potentially fatal. Studies focusing on the interaction between neotropical primates and the agents of this family are scarce. The present study aimed to identify agents of the Anaplasmataceae family in the whole blood of free-living and captive neotropical primates in the State of Mato Grosso, Central-West Brazil. Thirty-eight samples of six nonhuman primate (NHP) species were collected in seven municipalities and analysed through polymerase chain reaction (PCR), nucleotide sequencing, and phylogenetic analysis of the dsb, groEL, 16S rRNA, and gltA genes. DNA fragments similar to those of Ehrlichia canis were detected in Sapajus apella and Ehrlichia chaffeensis from Mico melanurus. The sequences generated in this study and homologous sequences retrieved from GenBank® were used for phylogenetic analyses to characterize the Ehrlichial agents detected in NHPs. The agents were then grouped into clades corresponding to different isolates from the NHP species. In addition, an Anaplasma sp. closely related to Anaplasma marginale was identified in two S. apella individuals. These findings shed light on the susceptibility of neotropical NHPs to Anaplasmataceae agents. These bacteria are known to be transmitted by ticks, which can also serve as possible sources of infection for other animals, including humans.

STYLAR MOVEMENT IN Talinum fruticosum (TALINACEAE): CONTRIBUTIONS TO THE REPRODUCTIVE SUCCESS OF THE SPECIES IN POPULATIONS FROM CENTRAL-WEST BRAZIL

In plant species, the movement of reproductive structures can be an adaptive advantage in mating system studies. This phenomenon can promote outcrossing or cause a reduction in intrafloral male-female sexual interference. Consequently, this contributes to the avoidance of self-pollination and/or delayed autonomous self-pollination. The aim of this short communication is to report the results of assessments done in two natural populations of a native species from central-west Brazil that grows spontaneously and sub-spontaneously in opening and ruderal areas of Pantanal. In this study, the movement of the stigma was recorded throughout floral anthesis, and the mating system was determined. Floral measurements such as: flower opening time, pollen viability and the period that the stigma was receptive were also reported. The formation of fruits by spontaneous self-pollination and cross-pollination reinforces the hypothesis that stylar movement in Talinum fruticosum can facilitate or hinder self-pollination in some situations.

Epidemiology of secondary infection after snakebites in center-west Brazil.

Snakebites represent a significant health problem in tropical countries, with an annual incidence of 2.7 million cases worldwide. The incidence of secondary infections after snake bites is also high and is usually caused by bacteria from the oral cavity of snakes. Morganella morganii has been identified as an important cause of infections and has been guiding antibiotic therapy in several regions of Brazil and the world. We performed a retrospective cross-sectional evaluation of snakebites in hospitalized patients between January 2018 and November 2019 and selected those with secondary infection in their medical records. During the period, 326 cases of snakebites were treated, and 155 (47.5%) of them eventually had secondary infections. However, only seven patients underwent culture of soft tissue fragments, in which three cases were negative culture results, while Aeromonas hydrophila was identified in four cases. Of these, 75% were resistant to ampicillin/sulbactam, 50% had intermediate sensitivity to imipenem, and 25% had intermediate sensitivity to piperacillin/tazobactam. Trimethoprim/sulfamethoxazole (TMP-SMX) was not tested on any strain. Of the 155 cases that progressed to secondary infections, 48.4% (75) were empirically treated with amoxicillin/clavulanate, 41.9% (65) with TMP-SMX, and 32 (22%) of these 144 cases required a change to a second regimen, and 10 of these 32 patients required a third therapeutic regimen. Wild animals act as reservoirs of resistant bacteria because their oral cavity favors biofilm formation, which explains the finding of A. hydrophila with a reduced sensitivity profile in this study. This fact is essential for the appropriate choice of empirical antibiotic therapy.

Associação entre Religião, Saúde Mental e Distanciamento Social na Pandemia de Covid-19

Abstract This cross-sectional study aimed to analyze the association of religiosity with behaviors and perceptions in the context of social distancing measures during the COVID-19 pandemic, as well as mental health outcomes, in a university community in Central-West Brazil. A sample of 1,796 subjects responded to an online form with socio-demographic questions and the DASS-21 and PWBS scales. Religion was associated with the frequency of interactions, perceptions of the duration of the social distancing measures, changes in emotional state and history of psychological illness. The prevalence of symptoms of depression, anxiety and stress was lower among people with religion and their scores in psychological well-being were higher.

Merkel cell polyomavirus (MCPyV) DNA prevalence in Brazilian blood donors.

Merkel cell polyomavirus (MCPyV) is an oncogenic virus that has been etiologically linked to Merkel cell carcinoma. Low levels of MCPyV DNA have been detected in blood donors with unclear impact on transfusion. The prevalence of MCPyV DNA in Brazilian blood donors is unclear. Therefore, the objective of this study was to evaluate the MCPyV DNA prevalence among Brazilian blood donors. We examined the presence of MCPyV DNA by real-time PCR (qPCR) in a total of 450 serum samples obtained from blood donors from three Brazilian regions (North, Central-West and South). The overall estimated MCPyV DNA prevalence was 1.1% (CI=95%, 0.16-2.06%). Divided by region, in North Brazil (city of Macapa, state of Amapá) and South Brazil (city of Santa Maria, state of Rio Grande do Sul), the MCPyV prevalence was the same: 1.33% (CI=95%, range 0.0-3.14%). In Central-West Brazil (city of Brasilia), the MCPyV prevalence was 0.6% (CI=95%, 0.0-1.96%). All MCPyV positive samples showed a high cycle threshold (median Ct=35.5), most probably related to the low viral load. More studies are necessary to unveil the impact of this oncogenic virus on transfusion medicine and if such exists, especially in regards of its infectivity and transmission potential.

Courtship, copulation, and territorialistic behaviors of Tropidurus torquatus (Tropiduridae) in a fragment of Cerrado in Central-West Brazil

This study observed and recorded courtship, copulation, and territorialistic behaviors in Tropidurus torquatus. Males of this species used a head bobbing or “push-up” display, which is the main communication sign with other males. Females, in turn, performed tail lifting, a specific sexual stimulus behavior indicating acceptance of copulation. Courtship took place with coordinated movements for both males and females and could result in effective copulation; females seemed to prefer dominant males during the reproductive period. Territorialistic behaviors occurred before and after courtship, demonstrating the efforts made by dominant males of T. torquatus to maintain their territory during the reproductive season.

Clinical-Epidemiological Characteristics and Outcomes of Latent Tuberculosis Treatment at a Tertiary Center in Central-West Brazil from 2017 to 2019.

Detailed information concerning latent tuberculosis infection (LTBI) and treatment outcomes is scarce in Brazil. This retrospective cross-sectional study aimed to describe LTB treatment (LTBT) at a tertiary center in Central-West Brazil from 2017 to 2019. We recommended the use of LTBTs before the implementation of a rifapentine-isoniazid (3HP) regimen in Brazil. We conducted a descriptive analysis using chi-square or t-tests to assess differences in the proportions and means. Of 79 notified adult patients (males, 68%; median age, 40 (interquartile range, 30-51) years), most people were living with human immunodeficiency virus (PLHIV) (82%) or receiving immunosuppressant medication (15%), and 92% were receiving their first treatment. Isoniazid (INH) for 6-9 months had previously been proposed for 95% of the patients, with only 35% completeness. Four patients treated with rifampicin (4RMP) completed the regimen (p = 0.009). Adverse events occurred in 19% of the patients. In this Brazilian tertiary center, the target population for LTBT were young PLHIV patients under immunosuppression with low education levels. However, the INH monotherapy dropout rate was 65%. Therefore, shorter courses, such as 3HP and 4RMP, are promising alternatives. Behavioral aspects, education level, and regimen length can influence the course completion, and further studies are required to evaluate the 3HP regime in Brazil.

First published record of rodent predation by guira cuckoo in Brazil: notes about vertebrate predation

Guira cuckoo (Guira guira) is a communal breeder bird species displaying a diversified diet. In this study, we report the first published predation event of a rodent by Guira cuckoo in Brazil. We searched for vertebrate predation by this bird species in Brazilian domains in the following databases: Google Scholar, SciELO, Scopus and Web of Science. We recorded the rodent predation event in a landscape composed of pasture and gallery forest in Campo Grande municipality, Mato Grosso do Sul, central-west Brazil. The predated rodent is a vesper mouse, Calomys sp. We found 19 vertebrate species predated by the Guira cuckoo, distributed in all Brazilian domains, within the orders: Anura (5 species), Passeriformes (1), Squamata (12), and Rodentia (1 - present study). Our result contributes to the natural history of the Guira cuckoo, and highlights that this cuckoo bird displays a generalist diet, feeding on a diversity of vertebrate species.

Glyphosate and aminomethyphosphonic (AMPA) contents in Brazilian field crops soils

Because of a lack of direct measurements, the presence and persistence of glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) in agricultural soils of Brazil remains unknown. This paper aims at reporting glyphosate and AMPA contents in Brazilian field crop soils at the country scale from southern states to eastern Amazon. Brazilian field crop farmers are among the highest users of glyphosate-based herbicides (GBH) in the world. Soybean and corn field crop soils from 17 sites were collected at two depths (i.e. 0-20 cm and 20-40 cm) in 2016 and 2018. The study area encompasses three regions of Brazil: southern Brazil, central-west Brazil and eastern Amazon, all having in common intensive, conventional, large-scale grain farming. ßGlyphosate and AMPA contents were measured using a GC-ECD following soil extraction. Both chemicals were ubiquitously detected in soils cultivated under various agricultural practices ranging from ploughing to no-till ones. Average measured glyphosate and AMPA contents were 0.08 ± 0.09 µg/g and 0.17 ± 0.16 µg/g respectively with maximum values of 0.57 µg/g and 0.98 µg/g. Glyphosate plus AMPA contents in the top 40 cm of soils presented cumulated amounts exceeding the annual glyphosate inputs via GBH. This is interpreted as a multiannual persistence of these chemicals in Brazilian agricultural soils. Downward migration of glyphosate and AMPA is also suspected with regard to the vertical distribution of these chemicals along with deeper soil horizons. This study highlights the backlash of conservation agriculture as far as glyphosate and AMPA presence in agricultural soils are concerned. These compounds persistence in Brazilian soils appear to be longer than what is usually reported in the literature, especially so for tropical environments. The worldwide concern about the ubiquitous presence of glyphosate and AMPA in the environment needs a broader screening in Brazilian field crop soils since the majority of the available data comes from Argentina, Europe and to a lesser extent North America.

Risk factors for potentially inappropriate medication use in older adults: a cohort study.

Much of the knowledge on the use of potentially inappropriate medications (PIM) in older adults is derived from cross-sectional studies, with little known about the risk factors over time. Longitudinal analysis was applied to estimate the occurrence and risk factors of PIM use among older adults in a 10-year follow-up. Longitudinal study with 418 older adult residents of a capital city of Central-West Brazil. The PIM were classified according to the Beers criteria 2019. The usage rate was calculated at baseline (2008) and at the 10-year follow-up moment (2018). Analysis of predictors (sociodemographic, self-rated health, hospitalization, number of comorbidities, polypharmacy, diabetes, hypertension, hypercholesterolemia and nutritional status) was performed using Generalized Estimating Equation (GEE) models. Mean age at baseline was 70.6years (SD 7.1) and 76% were women; 221 older adults took part in the follow up. The rate of PIM use was 50.4% at baseline and 57.5% at the 10-year follow-up. Multiple analysis showed that PIM use in the cohort was statistically higher in the older adults with a history of hospitalization (RRadj 1.20; 95% CI 1.01-1.40), with three or more diseases (RRadj 1.41; 95% CI 1.14-1.74), with polypharmacy (RRadj 1.81; 95% CI 1.47-2.24) and with diabetes mellitus (RRadj 1.24; 95% CI 1.05-1.47). A high level of potentially inappropriate medication use was observed, reaching 50% of the older adults, with a 7% increase in the prevalence over the 10-year follow-up period. Hospitalization, multimorbidities, polypharmacy and diabetes mellitus were associated with the use of these medications. Interventions for surveillance of the deprescribing process need to be encouraged to avoid potential harm caused by the use of medications.

Chromoblastomycosis-Leprosy Co-Infection in Central West Brazil. Presentation of Three Cases and Literature Review

Chromoblastomycosis and leprosy are chronic diseases with high prevalence in tropical and subtropical regions. Brazil is one of the countries with the highest incidence and prevalence for both diseases, however, reports of co-infections are scarce. The aim of this study was to describe three cases of chromoblastomycosis-leprosy co-infection in patients from Mato Grosso state, Brazil. A review of chromoblastomycosis-leprosy co-infection was performed of English, Portuguese and Spanish publications in LILACS, SciELO, PubMed and Web of Science databases using the descriptors (chromoblastomycosis OR cromoblastomicose OR cromoblastomicosis) AND (leprosy OR hanseníase OR lepra), without time period delimitation. Nineteen cases were included, 16 cases were published in 11 articles, plus the three cases reported in the current study. Most reported coninfection cases came from Brazil. Majority of the patients were male with a mean age of 52.2 years. Farmer was the main occupational activity reported. In 12 patients, the clinical signs and symptoms of leprosy started first. No contacts with patients affected by leprosy, armadillos or history of injuries at the anatomical site of chromoblastomycosis lesions were reported. Five leprosy patients who received steroid treatment for leprosy reactions or neuropathies, were diagnosed with chromoblastomycosis during immunosuppressive therapy. Four cases (21.1%) were reported among the elderly patients. Co-infections in patients with chromoblastomycosis or leprosy are uncommon, but the possibility should always be considered, especially if the patient is undergoing immunosuppressive treatment or is elder.

Socioeconomic disparities associated with symptomatic Zika virus infections in pregnancy and congenital microcephaly: A spatiotemporal analysis from Goiânia, Brazil (2016 to 2020).

The Zika virus (ZIKV) epidemic, which was followed by an unprecedented outbreak of congenital microcephaly, emerged in Brazil unevenly, with apparent pockets of susceptibility. The present study aimed to detect high-risk areas for ZIKV infection and microcephaly in Goiania, a large city of 1.5 million inhabitants in Central-West Brazil. Using geocoded surveillance data from the Brazilian Information System for Notifiable Diseases (SINAN) and from the Public Health Event Registry (RESP-microcefalia), we analyzed the spatiotemporal distribution and socioeconomic indicators of laboratory confirmed (RT-PCR and/or anti-ZIKV IgM ELISA) symptomatic ZIKV infections among pregnant women and clinically confirmed microcephaly in neonates, from 2016 to 2020. We investigated temporal patterns by estimating the risk of symptomatic maternal ZIKV infections and microcephaly per 1000 live births per month. We examined the spatial distribution of maternal ZIKV infections and microcephaly cases across the 63 subdistricts of Goiania by manually plotting the geographical coordinates. We used spatial scan statistics estimated by discrete Poisson models to detect high clusters of maternal ZIKV infection and microcephaly and compared the distributions by socioeconomic indicators measured at the subdistrict level. In total, 382 lab-confirmed cases of maternal ZIKV infections, and 31 cases of microcephaly were registered in the city of Goiania. More than 90% of maternal cases were reported between 2016 and 2017. The highest incidence of ZIKV cases among pregnant women occurred between February and April 2016. A similar pattern was observed in the following year, although with a lower number of cases, indicating seasonality for ZIKV infection, during the local rainy season. Most congenital microcephaly cases occurred with a time-lag of 6 to 7 months after the peak of maternal ZIKV infection. The highest estimated incidence of maternal ZIKV infections and microcephaly were 39.3 and 2.5 cases per 1000 livebirths, respectively. Districts with better socioeconomic indicators and with higher proportions of self-identified white inhabitants were associated with lower risks of maternal ZIKV infection. Overall, the findings indicate heterogeneity in the spatiotemporal patterns of maternal ZIKV infections and microcephaly, which were correlated with seasonality and included a high-risk geographic cluster. Our findings identified geographically and socio-economically underprivileged groups that would benefit from targeted interventions to reduce exposure to vector-borne infections.

Bioeconomics considerations on the modal production system of beef cattle in Pantanal / Considerações bioeconômicas sobre o sistema de produção modal do gado de corte no Pantanal

Beef cattle production is one of the main agricultural activities in Brazil accounting for approximately 75% of agricultural properties in the country and significantly impacting gross domestic product (GDP) figures. However, raising beef cattle is a complex activity, with great variability in the combination of production factors. In this study, we assessed scale gains in beef cattle and performed a temporal analysis of the modal system in the Pantanal biome, central-west Brazil. Beef cattle production systems were analyzed for Corumbá, state of Mato Grosso do Sul. Typical farms of 3,600 hectares (ha), 9,000 ha, 14,400 ha, and 30,000 ha were determined from primary data from focus group surveys conducted by CEPEA. Results showed that the smallest property was the most efficient in using natural resources and had the highest economic return relative to the effective operational cost (EOC), followed by the largest property. Profitability increased significantly over the years due to record calf prices in 2014 and increased production. Intermediate-sized properties had the worst economic results and negative net margin even when the producer’s pro-labore is not considered.

The role of prisons in disseminating tuberculosis in Brazil: A genomic epidemiology study

SummaryBackgroundGlobally, prisons are high-incidence settings for tuberculosis. Yet the role of prisons as reservoirs of M. tuberculosis, propagating epidemics through spillover to surrounding communities, has been difficult to measure directly.MethodsTo quantify the role of prisons in driving wider community M. tuberculosis transmission, we conducted prospective genomic surveillance in Central West Brazil from 2014 to 2019. We whole genome sequenced 1152 M. tuberculosis isolates collected during active and passive surveillance inside and outside prisons and linked genomes to detailed incarceration histories. We applied multiple phylogenetic and genomic clustering approaches and inferred timed transmission trees.FindingsM. tuberculosis sequences from incarcerated and non-incarcerated people were closely related in a maximum likelihood phylogeny. The majority (70.8%; 46/65) of genomic clusters including people with no incarceration history also included individuals with a recent history of incarceration. Among cases in individuals with no incarceration history, 50.6% (162/320) were in clusters that included individuals with recent incarceration history, suggesting that transmission chains often span prisons and communities. We identified a minimum of 18 highly probable spillover events, M. tuberculosis transmission from people with a recent incarceration history to people with no prior history of incarceration, occurring in the state’s four largest cities and across sampling years. We additionally found that frequent transfers of people between the state’s prisons creates a highly connected prison network that likely disseminates M. tuberculosis across the state.InterpretationWe developed a framework for measuring spillover from high-incidence environments to surrounding communities by integrating genomic and spatial information. Our findings indicate that, in this setting, prisons serve not only as disease reservoirs, but also disseminate M. tuberculosis across highly connected prison networks, both amplifying and propagating M. tuberculosis risk in surrounding communities.FundingBrazil’s National Council for Scientific and Technological Development and US National Institutes of Health.

Abiotic drivers shape seed inputs and outputs in a tropical wetland on Croton trinitatis population

BackgroundPredicting how natural and anthropogenic drivers shape different ecological indicators, such as plant populations along environmental gradients, can be a relevant tool for establishing management and conservation criteria of tropical wetlands. We aimed to assess the effects of seasonal flood disturbance, type of grasslands and topographical conditions on Croton trinitatis population distribution in a tropical wetland.MethodsThe study was carried out in a seasonally flooded grassland (Central-West Brazil). We conducted samplings of soil on the dry and flood hydrophases of the Pantanal. We took the samples in eight seasonal ponds, with 1 km interval between them. Transects were marked during the flood period, observing the water level, one in the lowest zone, in the middle of the pond = low (ca. 60 cm deep), one at the pond edge = mid (ca. 30 cm deep) and one in the higher zone, on the external part = high (ca. 1 cm deep).ResultsThe results showed that the topography, seasonality, and types of grassland determine differences in the abundance patterns of adult plants and seedlings, and seed bank and seed predation. The abiotic factors can shape plant population-related ecological processes and patterns, with outputs (germination and predation) and inputs (local dispersion and from neighbouring areas) of proportional seeds for the population maintenance in this environment.ConclusionWe emphasize the importance of these findings, to show that abiotic factors are not the only ones to be considered in ecological studies of distribution and structuring of populations in habitats with extreme seasonal events.

Continental freshwater carbonate coated grains: oncoids in Quaternary deposits of the Serra da Bodoquena region, Central-West Brazil

Continental freshwater carbonate coated grains: oncoids in Quaternary deposits of the Serra da Bodoquena region, Central-West Brazil

Phytoseiidae mites associated with native and cultivated solanaceous in Central-West Brazil

Predatory mites represent important biological control agents and those belonging to the Phytoseiidae family are the most promising for the control of phytophagous mites and small insects. The control of key pests of tomato and other solanaceous crops, highlighting phytophagous mites, has been a challenge and the biological control constitutes a promising strategy. Prospecting predatory mites in wild host plants, natural environments as well as in agroecosystems is relevant because these non-crop and crop areas can serve as reservoirs for promising species for biological control programs. This study aimed to know the Phytoseiidae fauna associated with wild and cultivated solanaceous plants in a poorly prospected area in Brazil, the Central-West Region. A detailed taxonomic identification of phytoseiid mites was conducted, and the most important morphological traits are presented for each species. In addition, associated phytophagous mites mainly belonging to the Tetranychidae, Tenuipalpidae and Eriophyoidea were identified. Surveys were carried out in 23 species of solanaceous collected in the Distrito Federal (12 areas) and Goiás State (1 area), from February 2017 to January 2018. Nineteen species of predators belonging to ten genera were recorded: Amblyseius (2 species), Euseius (3), Galendromus (1), Iphiseiodes (1), Neoseiulus (3), Paraphytoseius (1), Phytoseius (3), Proprioseiopsis (2), Typhlodromalus (2, one probably new to science) and Typhlodromips (1). Solanum lycocarpum was the solanaceous that harbored the highest richness (11 species), as well as the one with the highest abundance of phytoseiids (250 specimens). Typhlodromalus aripo was the most common species, being the most abundant (423 specimens; 32%) and registered on the largest number of hosts (14). Many of phytoseiid species found present morphological traits that facilitate their occurrence in leaves with trichomes, as in the case of tomato and other cultivated solanaceous. These traits and the association between predators and phytophagous mites may indicate that these species are promising for biological control programms. Thus, extensive studies to assess the efficiency of the identified predatory mites to control key solanaceous pests are required.

Inequality of gender, age and disabilities due to leprosy and trends in a hyperendemic metropolis: Evidence from an eleven-year time series study in Central-West Brazil.

The present study aimed to investigate the epidemiological situation of leprosy (Hansen's Disease), in a hyperendemic metropolis in the Central-West region of Brazil. We studied trends over eleven years, both in the detection of the disease and in disabilities, analyzing disparities and/or differences regarding gender and age. This is an ecological time series study conducted in Cuiabá, capital of the state of Mato Grosso. The population consisted of patients diagnosed with leprosy between the years 2008 and 2018. The time series of leprosy cases was used, stratifying it according to gender (male and female), disability grade (G0D, G1D, G2D, and not evaluated) and age. The calendar adjustment technique was applied. For modeling the trends, the Seasonal-Trend decomposition procedure based on Loess (STL) was used. We identified 9.739 diagnosed cases, in which 58.37% were male and 87.55% aged between 15 and 59 years. Regarding detection according to gender, there was a decrease among women and an increase in men. The study shows an increasing trend in disabilities in both genders, which may be related to the delay in diagnosis. There was also an increasing number of cases that were not assessed for disability at the time of diagnosis, which denotes the quality of the services.

Molecular epidemiological investigation of Mayaro virus in febrile patients from Goiania City, 2017-2018.

Mayaro virus (MAYV) has historically been associated with sylvatic transmission; however, urban outbreaks have been reported in Brazil, including cases of co-detection with dengue virus (DENV). Therefore, we performed a molecular survey to investigate MAYV circulation and cocirculation with DENV within Goiania, a major city in Central-West Brazil. Among 375 subjects with arbovirus-like symptoms, 259 were positive for DENV and 26 for MAYV. Of these, 17 were coinfected with DENV-2, suggesting co-transmission of the viruses. The most common complaints at the time of inclusion were myalgia, headache, fever, arthralgia, retro-orbital pain, and skin rash. No specific symptoms were associated with MAYV when either detected alone or co-detected with DENV, compared to that when DENV was detected alone. Most MAYV-infected subjects were women with no recent travel history to rural/sylvatic areas. Phylogenetic reconstruction indicated that the MAYV identified in this study is closely related with a lineage observed in Peru, belonging to genotype D. Our results corroborate the growing circulation of MAYV in urban environments in Brazil and reinforce the need to implement laboratory diagnosis in the Unified Health System, considering that the clinical manifestations of Mayaro fever are similar to those of other arboviruses, particularly dengue. Furthermore, most cases occurred in association with DENV-2. Further phylogenetic studies are needed to evaluate MAYV, which has not been widely examined.

Immigration as practice of organization: discussions about practices of organization, displacement, and integration of Haitian immigrants in the Goiânia Metropolitan Region, in Goiás, Brazil

Abstract This study aims to discuss the practices of displacement and organization that are part of the process of integrating Haitian immigrants in the countries that they live, in their diaspora process. Haiti is a country historically marked by displacement, which is observed since the colonial period, when people were forcibly displaced to be enslaved. This article will focus on the process of displacement, through the theoretical lens of Practice-Based Studies, using Michel de Certeau’s theory about social practices, associated with the concepts of Tim Cresswell about displacement and immigration as practice. The research was conducted between August and December 2017 in two Haitian communities in the Goiânia Metropolitan Region, in the state of Goiás, located in Central-West Brazil. For the production of empirical material, we used the techniques of life history interview and participant observation, by accompanying the everyday life activities in the immigrant’s communities. The results show that the practice of displacement of Haitian immigrants is an unfinished and still in process phenomenon, involving different dimensions of events, with international, national, and commuting displacement. It was also possible to conclude that the displacement practices are permeated by organizational practices and that “organizing” and “migrating” are processes involved. In addition, the practice of organization is a key component for integration and access to the workplace in Brazil. Therefore, immigration can be considered sets of practices to organize mobility experiences that are spatially multi-localized and incorporated.