p150Glued(Dynactin 1) is a component of the dynactin complex that is essential for retrograde axonal transport in neurons. The mouse p150GluedcDNA was isolated from mouse brain by RT-PCR. The complete sequence of the full length mouse cDNA was determined, including 19 bp of 5′ UTR, 3843 bp of coding sequence, and 162 bp of 3′ UTR. The predicted protein has a molecular mass of 142 kDa and exhibits 95% amino acid sequence identity to the predicted amino acid sequence of human p150Glued(DCTN1). The mouseDctn1gene was previously mapped in the central region of mouse chromosome 6, close to the neuromuscular disease genemnd2.Northern blot analysis, complete sequencing of the cDNA, Western blot, and functional tests of the protein did not detect any abnormalities of p150Gluedinmnd2mice.