The nitrogen and phosphorus accumulation in different tree parts (including root systems) of Eucalyptus camaldulensis Dehnh., Eucalyptus urophylla Blake and Eucalyptus pellita F. Muell. planted at three spacings (3 m × 1.5 m, 3 m × 3 m and 4 m × 3 m) and at three ages (15, 31 and 41 months) were evaluated in the savanna region of central Minas Gerais state in southeastern Brazil. A series of equations were produced to estimate per-tree nitrogen and phosphorus from age, spacing, diameter and height, and per-hectare nitrogen and phosphorus using age, spacing and a tally of tree diameters and heights. The highest N and P concentrations were observed in foliage (15–23 g N kg −1 and 0.8–1.1 g P kg −1) and the lowest N concentration in bolewood (2.4–4.1 g N kg −1), while the lowest P concentration was observed in the taproot (0.16–0.29 g P kg −1). Total biomass N at age 41 months was greatest in E. urophylla (378–457 kg N ha −1), lower in E. pellita (238–326 kg N ha −1) and lowest in E. camaldulensis stands (204–240 kg N ha −1), depending on spacing. Total biomass P at age 41 months was also greatest in E. urophylla (16.6–21.8 kg P ha −1), and about equal in E. pellita (10.4–12.8 P ha −1) and E. camaldulensis stands (10.4–12.2 kg P ha −1), depending on spacing. As age and spacing increased, individual stems increased in diameter and total N and P, but the relationship between total N and P pools and age and spacing was more variable.