Central Italy Research Articles

Evaluating small‐scale harvesting disturbance to the forest soil in Mediterranean beech high forests

AbstractEuropean beech (Fagus sylvatica L.) forests in Central and Southern Italy are managed applying the shelterwood system. Prior to the regeneration cut, it is common to apply 2–3 thinning interventions, aiming to obtain mostly firewood, considering the low dimension and poor quality of the stems. These interventions are usually carried out by local forest enterprises relying on a low or medium level of mechanization (small‐scale forestry). In particular, the short wood system is applied, thus processing the logs to 1 m length and extracting them with forestry‐fitted farm tractors equipped with forwarding bins. Despite the large application of this harvesting system in the Mediterranean forestry, no information is available in the literature about its possible disturbance to the forest soil. To fulfill this knowledge gap, we developed the first assessment of soil physicochemical (bulk density, penetration resistance, shear resistance, organic matter content) and biological (soil microarthropods biodiversity evaluated with the QBS‐ar index, that is, an index based on the idea that high‐quality soils have more groups of microarthropods that are morphologically better adapted to the soil than low‐quality soils) properties for this kind of logging operation. In three case study areas in Central Italy, we applied an experimental design to evaluate separately the impacts related to the passage of the machine and that of the silvicultural treatment itself. We further applied linear mixed‐effect models to investigate the relationship between changes in soil physicochemical and biological properties. We found the effect of the silvicultural treatment to be negligible, but there was a significant alteration of the investigated parameters in the soil affected by the passage of the machine. Soil penetration and shear resistance doubled in the forwarding trails (0.25 MPa and 4.02 t m−2, respectively) in comparison with the other two experimental treatments (control area and soil not affected by the machine passage; about 0.12 MPa and 2.10 t m−2, respectively). Soil organic matter and soil microarthropod biodiversity (QBS‐ar index) were reduced by 25% in the forwarding trails (about 30% and 92 respectively) in comparison with the soil not affected by the machine passage (about 39% and about 130, respectively). Such significant disturbance, which occurred even if the applied machinery had lower weight in comparison to other alternatives (forwarders), is related to the high number of machine passes needed to extract the woody material with forwarding bins applying the short wood system. We found a significant relationship between soil compaction and soil organic matter removal and microarthropod biodiversity, with the latter which resulted significantly lower in more compacted and organic matter‐poor soils. We demonstrated that this small‐scale extraction system can have a negative effect on soil features in the short term. We therefore recommend the application of best management practices, for instance placing brush mats and logging residues, on the forwarding trails to limit the soil disturbance within the framework of small‐scale forestry. We further recommend a wider application of aerial harvesting systems, which generally have lower impacts to the soil. However, this could happen only after an extensive training program aimed to increase the knowledge and skills of local loggers about aerial harvesting systems.

SARS-CoV-2 Molecular Evolution: A Focus on Omicron Variants in Umbria, Italy.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 6 million deaths worldwide, and the spread of new variants over time increased the ability of this virus to cause infection. The Omicron variant was detected for the first time in Umbria, a region of central Italy, in November 2021 and it induced an unprecedented increase in the number of infection cases. Here, we analysed 3300 SARS-CoV-2 positive samples collected in Umbria between April 2022 and December 2023. We traced the molecular evolution of SARS-CoV-2 variants over time through the Next-Generation Sequencing (NGS) approach. We assessed correlation between SARS-CoV-2 infection and patients' health status. In total, 17.3% of our samples came from patients hospitalised as a consequence of COVID-19 infection even though 81.4% of them received at least three vaccine doses. We identified only Omicron variants, and the BA.5 lineage was detected in the majority of our samples (49.2%). Omicron variants outcompeted each other through the acquisition of mutations especially in Spike glycoprotein that are fingerprints of each variant. Viral antigenic evolution confers higher immunological escape and makes a continuous improvement of vaccine formulation necessary. The continuous update of international genomic databases with sequencing results obtained by emergent pathogens is essential to manage a possible future pandemic.

Back to black: Analysis of the earliest natron glass found in Italy

An assemblage of black-appearing glass objects (predominantly beads) that is dated to the 9th–7th centuries BCE and found in Iron Age contexts of Southern Etruria and Latium (central Italy) was analysed using portable X-ray fluorescence spectrometry, laser ablation – inductively coupled plasma – mass spectrometry and micro-Raman spectroscopy. The analytical approach allowed us to discuss the technology of glass-making in a period of relevant technological changes. It resulted that the glass that reached the Italian peninsula was somewhat different, from a compositional point of view, from both earlier and later periods. The data collected showed that iron – the main colouring agent for these glasses – was obtained from several sources as was silica – the main component of glass. Lime, one of the main constituents of many types of glass, plays a modest role in this assemblage, which places these samples in a special position because of the peculiar batch formula. At least three centres of raw glass production are suggested by the compositional data, but in each case the glass was imported to the Italian peninsula.

Tsunami at school. An experimental strategy to increase students’ risk perception

We tested a methodology for assessing high school students’ tsunami risk perception and knowledge to improve their tsunami awareness. Our objective was to test whether the use of a structured questionnaire on tsunami risk perception could be used as a tool to assess students’ improvement after a lesson on tsunamis. For this purpose, we involved about 100 students from four classes of a high school (Polo Liceale Statale “Lazzaro Spallanzani”) in the town of Tivoli, in central Italy, about 20 km from Rome and 40 km from the seaside. We administered the questionnaire in three subsequent surveys (to 94, 96, and 109 students, respectively), the first of which without providing any information on which subject they would be dealing with. Students were asked to answer the 31 questions individually, each using her/his mobile phone during science class. After two months, all four classes were trained with a frontal lesson and a discussion on tsunami risk. Afterwards, we administered the questionnaire again and ran the second survey. Finally, we met the students one year later and administered the questionnaire again, then we compared the three survey results. The comparison shows that the students’ risk knowledge and perception, which was generally very low in the first survey, improved significantly after the lesson and the relative discussion. Although this result was somewhat expected, it has been possible to confirm and quantify the improvement. According to studies conducted using similar methods [Gray and Di Loreto, 2016; Ayllón et al., 2019], the active participation of students, the collaborative approach provided by teachers and researchers, and the use of a survey tool such as a questionnaire – which, despite being completed individually, fosters an environment of emotional sharing – all contribute to stimulating students’ curiosity and facilitating their learning in traditional educational settings. We then administered the questionnaire to the same classes one year later. The outputs of the third survey show a partial decrease in students’ awareness that nevertheless remains significantly better than in the first survey. This suggests that the students’ involvement in the learning process through the questionnaire and through an active discussion after watching videos and pictures works well, but also that a periodic reminder by the science teachers is needed to maintain a constant level of risk awareness and perception. We propose to replicate this approach by involving teachers in the survey, providing them with a recorded lesson with all the basic elements, to be shown between the first and second administration of the questionnaire, and with periodic (yearly) reminders.

Mutational Landscape of Alzheimer's Disease and Frontotemporal Dementia: Regional Variances in Northern, Central, and Southern Italy.

Alzheimer's Disease (AD) and Frontotemporal Dementia (FTD) are the two major neurodegenerative diseases with distinct clinical and neuropathological profiles. The aim of this report is to conduct a population-based investigation in well-characterized APP, PSEN1, PSEN2, MAPT, GRN, and C9orf72 mutation carriers/pedigrees from the north, the center, and the south of Italy. We retrospectively analyzed the data of 467 Italian individuals. We identified 21 different GRN mutations, 20 PSEN1, 11 MAPT, 9 PSEN2, and 4 APP. Moreover, we observed geographical variability in mutation frequencies by looking at each cohort of participants, and we observed a significant difference in age at onset among the genetic groups. Our study provides evidence that age at onset is influenced by the genetic group. Further work in identifying both genetic and environmental factors that modify the phenotypes in all groups is needed. Our study reveals Italian regional differences among the most relevant AD/FTD causative genes and emphasizes how the collaborative studies in rare diseases can provide new insights to expand knowledge on genetic/epigenetic modulators of age at onset.

A Eurasian otter <i>Lutra lutra</i> (Linnaeus 1758) (Carnivora: Mustelidae) in the Tiber basin near Rome: relict or range expansion?

The Eurasian otter Lutra lutra faced a significant decline in Italy during the 20th century, leading to local extinctions. After fifty years of otter disappearance from the Tiber basin, this study documents a sighting near Rome, obtained during a long-term trail camera monitoring. The study area, Rio Moscio, offers a habitat rich in hygrophilous vegetation and diverse fauna, including other protected species such as the Italian wolf, the European wildcat, and the European polecat. The observed otter could either mark a potential range expansion or could challenge the presumed extinctions in this region, since it could be in dispersal from the closer known populations or an individual from a Tiber basin relict population. The study emphasises the need for continued monitoring and prompt future genetic analyses to obtain necessary insights for conserving otters in Central Italy.

Molecular Survey on the Occurrence of Tick-Borne Bacteria in Wild Birds from Central Italy.

Birds are known to be carriers of ticks infected by tick-borne pathogens, including bacteria. However, not many studies have been carried out on avian tissues to detect these agents. The aim of the present survey was to investigate, using PCR, the presence of Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Chlamydia psittaci, Coxiella burnetii, Ehrlichia canis, Francisella tularensis, and Rickettsia spp. in the spleens collected from 300 wild birds of different orders and species from Central Italy. A total of 53 (17.67%) samples were PCR positive for at least one investigated pathogen. One (0.33%) bird was positive for Bartonella spp., five (1.67%) birds were positive for C. burnetii, eleven (3.67%) for B. burgdorferi s.l., and thirty-six (12%) for C. psittaci. No coinfection was detected. All samples were negative for A. phagocytophilum, E. canis, F. tularensis, and Rickettsia spp. The findings showed that wild birds may harbor different zoonotic tick-borne bacteria; therefore, they can contribute to the diffusion of these agents.

Simultaneous mating disruption of two moth pests of the vineyard (Lobesia botrana and Cryptoblabes gnidiella) through a biodegradable sex pheromone dispenser

Lobesia botrana (Lepidoptera: Tortricidae) and Cryptoblabes gnidiella (Lepidoptera: Pyralidae) represent a threat to wine production in Mediterranean countries. In recent years, the development of new formulations promoted the spread of pheromone-based mating disruption (MD) as an effective tool for the management of several insect pests in different agricultural contexts. In this study, we investigated the efficacy of an experimental dispenser designed for simultaneous MD of these two pests. The biodegradable double-tube dispenser (Isonet® L CG-BIOX235) was tested for two years in two Italian wine-growing sites, the first in Apulia (Southern Italy), and the second in Tuscany (Central Italy). Isonet® L CG-BIOX235 efficacy was evaluated by testing different doses (i.e., 300, 400, and 500 dispensers/ha), on different varieties (i.e., Aglianico, Syrah, and Viognier), and comparing it with an untreated control. The MD performed using this dispenser significantly reduced the infestation of both L. botrana (i.e., percentage of infested bunches and number of nests per bunch) and C. gnidiella compared to the untreated control, although the occurrence of the latter fluctuated throughout the two-year trials. Overall, although our results underline the possibility of combining the pheromones of the two pests in a single dispenser for their simultaneous MD, they also highlight the need for further studies on some aspects of C. gnidiella biology and consequently improve the MD efficacy against this species.

A strainmeter array as the fulcrum of novel observatory sites along the Alto Tiberina Near Fault Observatory

Abstract. Fault slip is a complex natural phenomenon involving multiple spatiotemporal scales from seconds to days to weeks. To understand the physical and chemical processes responsible for the full fault slip spectrum, a multidisciplinary approach is highly recommended. The Near Fault Observatories (NFOs) aim at providing high-precision and spatiotemporally dense multidisciplinary near-fault data, enabling the generation of new original observations and innovative scientific products. The Alto Tiberina Near Fault Observatory is a permanent monitoring infrastructure established around the Alto Tiberina fault (ATF), a 60 km long low-angle normal fault (mean dip 20°), located along a sector of the Northern Apennines (central Italy) undergoing an extension at a rate of about 3 mm yr−1. The presence of repeating earthquakes on the ATF and a steep gradient in crustal velocities measured across the ATF by GNSS stations suggest large and deep (5–12 km) portions of the ATF undergoing aseismic creep. Both laboratory and theoretical studies indicate that any given patch of a fault can creep, nucleate slow earthquakes, and host large earthquakes, as also documented in nature for certain ruptures (e.g., Iquique in 2014, Tōhoku in 2011, and Parkfield in 2004). Nonetheless, how a fault patch switches from one mode of slip to another, as well as the interaction between creep, slow slip, and regular earthquakes, is still poorly documented by near-field observation. With the strainmeter array along the Alto Tiberina fault system (STAR) project, we build a series of six geophysical observatory sites consisting of 80–160 m deep vertical boreholes instrumented with strainmeters and seismometers as well as meteorological and GNSS antennas and additional seismometers at the surface. By covering the portions of the ATF that exhibits repeated earthquakes at shallow depth (above 4 km) with these new observatory sites, we aim to collect unique open-access data to answer fundamental questions about the relationship between creep, slow slip, dynamic earthquake rupture, and tectonic faulting.

Damage‐informed ground motion and semi‐empirical fragility assessment

AbstractCalibrating parametric fragility curves via empirical damage data is one of the standard approaches to derive seismic structural vulnerability models. Fragilities based on empirical data require the characterization of the ground motion (GM) intensity at the building sites in the area affected by the earthquake producing the observed damages. This is commonly conducted via ShakeMap, that is, a map of the expected values of a Gaussian random field (GRF) of the logarithms of a GM intensity measure conditional to magnitude, location, and possibly a set of recordings of the earthquake. Once that intensity and damage data at the same sites are available, the typical approach calibrates a two‐parameter fragility model. However, ShakeMap estimates are affected by uncertainty deriving from that of the GM model used to characterize it. Furthermore, such an uncertainty can be reduced by building damage data, which provide information on the shaking intensity at the sites where damage is observed. It is shown herein that if this uncertainty is not addressed, also considering the shaking information provided by damage, the estimates of the fragility parameters obtained using a median ShakeMap only can be biased, and a recommended maximum likelihood estimation procedure – which exploits the expectation maximization algorithm – is provided. These arguments are illustrated via an application considering damage data from the 2009 L'Aquila earthquake in central Italy.

Non-native common snapping turtle Chelydra serpentina (Linnaeus, 1758) in Western Europe: a focus on Central Italy showing evidence of a hot spot of introduction

The common snapping turtle, Chelydra serpentina (Linnaeus, 1758), is a robust aquatic turtle native to southeast North America. However, it has also been introduced to other countries through the exotic pet trade, where it has gained popularity due to its distinctive appearance and impressive size. Over the past decade, a considerable number of individuals, abandoned by their owners, have been recorded and captured in Western Europe. Here, we report the first comprehensive summary of records at continental scale, showing that France and Italy represent the countries with the highest number of sightings. In France, exhibition, exchange, or sale events of exotic animals (until 2022 legal; now illegal) have been located near large cities, although records of C. serpentina were significantly higher outside the core of urban areas. We also recorded a significant increase in total number of records at European scale when comparing the periods of 2010-2015 and 2016-2020. Finally, we focused the current study on a peculiar spatial pattern at local scale (Central Italy) near an important site of exhibition and sale events of exotic animals, suggesting the presence of a hot spot of introduction. Popular events may represent critical colonization hubs from where these freshwater turtles can disperse into the surroundings.

Multicriteria decision tools for selection of sustainable integrated retrofits: application to the seismic and energy upgrade of a masonry building

The design of sustainable integrated retrofits is a new frontier in civil engineering that aims to minimize economic and environmental impacts of interventions on existing buildings. The enhanced methodology presented in this paper combines seismic and energy performance metrics with economic and environmental indicators associated with each integrated retrofit. The purpose is to identify the optimal retrofit through histograms, radar charts, proficiency ratios and iso-class plots, as the one corresponding to the higher seismic and energy performances and the minimum economic and environmental impacts. Such a methodology can be applied to either the installation phase of the retrofit or its entire life cycle, making it possible to compare these two scenarios in a long-term perspective. The methodology, generally valid for any building materials or structural typologies, is here applied to a two-story natural-stone masonry building, damaged by the 2016 Central Italy earthquake sequence.

On the occurrence of kalsilite in melilite-bearing ultrapotassic lavas from the Roman Province (Vulsini Mts., central Italy)

The Montefiascone Volcanic Complex belongs to the Vulsini Mts. volcanic district, one of the several leucite-bearing ultrapotassic Pleistocene volcanoes of the Roman Province (Central Italy). It is characterized by abundant pyroclastic successions and minor lava flows emplaced during a caldera formation phase occurred ∼0.3–0.2 Ma. The volcanic products span from leucite-basanites and leucite-tephrites to rarer leucitites and melilitites, occasionally associated with very rare kalsilite melilitolite ejecta. Here we report for the first time the presence of primary kalsilite in two lava flows in Forcinella and Feltricci localities, identifying a kamafugitic component among the Montefiascone volcanics.Detailed petrographic, SEM and EPMA studies on ten samples from the two localities revealed, in addition to kalsilite, variable amounts of clinopyroxene, melilite, leucite and nepheline plus rarer olivine coupled with carbonate-bearing segregation pockets in the groundmass. Geochemical and isotopic data confirm the exotic nature of the analysed rocks, showing ultrabasic/basic (SiO2 = 42.15–45.94 wt%) and ultrapotassic (K2O = 6.17–8.48 wt%; K2O/Na2O = 5.3–8.7) compositions, as well as strongly radiogenic 87Sr/86Sr (0.71038–0.711068), poorly radiogenic 143Nd/144Nd (0.512057.512091) and uniform middle radiogenic 206Pb/204Pb (18.76–18.78). Boron isotopes (δ11B ranges from 7.36 to 8.82‰) almost overlaps the typical MORB-OIB range values.These geochemical features recall those of the nearby kamafugite rocks from the Intra-Apennine Province. Major oxide and trace element modelling support fractionation links among the Italian kamafugite types. The overall geochemical and isotopic signatures speak for a common strongly subduction-metasomatized and orthopyroxene-free lithospheric mantle source. As already proposed for some nearby kamafugite centres in central Italy, limestone assimilation during magma ascent is a viable option to explain the mineralogical and whole-rock composition of Montefiascone lavas (i.e., the presence of carbonate plagues in the lava groundmass). This is particularly relevant considering the thick sedimentary sequence pierced by the limited volume of the upwelling magma. On the other hand, many other key features (such as the general lack of crustal xenoliths, the overall incompatible element fractionation and the radiogenic Sr isotopic ratios) clearly require also derivation from subduction-modified mantle sources.

Secondary metabolites of Araucaria cunninghamii Mudie from central Italy

In this paper, the first complete phytochemical analysis of an Italian specimen of Araucaria cunninghamii Mudie led to the identification of six secondary metabolites, one of which was identified in the species for the first time. The chemophenetic and ethnobotanical evaluations of this specimen are widely discussed.

Effects of Forest Management on Beetle (Coleoptera) Communities in Beech Forests (Fagus sylvatica) in the Apennines of Central Italy (Tuscany)

In European mountains most beech forest areas have been managed for timber production. This practice has reduced the availability of biomass for the whole forest-dwelling species assemblage and of deadwood for the saproxylic community. Despite most of Italy’s beech stands having a long history of management, its effects on forest species remain poorly understood. To address this gap, we studied beetle abundance and diversity in five beech-dominated forests with increasing management intensity in central Italy’s Apennines (Tuscany). We assessed if forests with similar management intensity exhibited comparable patterns in beetle diversity, abundance, and commonness versus rarity. Three forests were managed with even-aged shelterwood; one was managed with continuous cover forestry; and one was old-growth. We found 25 beetle families and 195 species across all sites with similar total abundance and richness. However, the representation of the most abundant families varied among sampling sites (ANOVA test: always significant for the total abundance of the most abundant families: F ≥ 2.77, d.f. = 4, p ≤ 0.038). The old-growth forest harbored more threatened species than managed sites. Saproxylic assemblages were similar between the recently cut site and the old-growth forest, and between shelterwood and continuous cover sites. While the similarity gradient among the whole species assemblages reflected geographical proximity, the similarity gradient among saproxylic assemblages reflected the successional proximity among forest management systems. Our research underscores the effects of management on beetle diversity, offering insights for sustainable forestry.

Low Effectiveness of Mid-Infrared Spectroscopy Prediction Models of Mediterranean Italian Buffalo Bulk Milk Coagulation Traits.

This study evaluated the potential use of mid-infrared spectroscopy to predict milk coagulation traits in bulk milk from Mediterranean Italian buffaloes. A total of 1736 bulk milk samples from 55 farms in central Italy were collected during the official milk quality testing system. The prediction models were developed based on modified partial least-squares regression with 75% of the samples and validated with the remaining samples. All bulk milk samples coagulated between 7.37 and 29.45 min. Average values for milk coagulation traits in the calibration set were 17.71 min, 3.29 min, and 38.83 mm for rennet coagulation time, curd firming time, and curd firmness, respectively. The validation set included samples with similar mean and standard deviation for each trait. The prediction models showed the greatest coefficient of determination of external validation (0.57) and the ratio of prediction to deviation (1.52) for curd firmness. Similar fitting statistics of the prediction models were obtained for rennet coagulation time and curd firming time. In conclusion, the prediction models for all three coagulation traits were below the threshold to consider the prediction models adequate even for rough screening of the samples.

Insights into Notonecta maculata biology and its consumptive and non‐consumptive effects on Aedes albopictus

AbstractNotonecta species (Hemiptera: Notonectidae) are generalist true predators with a strong impact on the size, structure, and richness of the aquatic communities, particularly on mosquito populations. Juvenile and adult backswimmers not only prey on mosquito larvae (consumptive effect) but their presence can also deter the oviposition of gravid mosquito females (non‐consumptive effect). In this study, we selected a Western Palearctic backswimmer, Notonecta maculata Fabricius, as a predator and deterrent of the highly invasive and health‐threatening Asian tiger mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae). In central Italy, N. maculata is univoltine and overwinters in the adult stage. Females lay eggs from February to the end of May, and the first new adults will emerge in late June; the complete nymphal development requires 44.5 ± 3.1 days. The number of Ae. albopictus larvae preyed on by N. maculata during its growth increases while developing, going from 0.03 ± 0.02 larvae per day for first instar nymphs to 21.38 ± 2.90 for mature adults. Furthermore, the presence of a single N. maculata adult in a mosquito ovitrap is enough to discourage the Ae. albopictus oviposition for up to 5 days. Limiting mosquito populations and, consequently, the transmission of viruses and parasites should be our main goal. Therefore, the integrated management of Ae. albopictus by the use of insecticides and a predator (such as backswimmer juveniles and adults), even if only in specific situations, may be promising and feasible. A more in‐depth knowledge of the biological cycle of N. maculata could help, consequently, to optimize its mass rearing.

Micro-habitat shifts by butterflies foster conservation strategies to preserve pollinator diversity in a warming Mediterranean climate

The Butterfly Monitoring Scheme based on linear transects conducted by citizen scientists has become a fundamental indicator for understanding the impact of climate change on butterfly populations. A key finding is the fast poleward expansion of butterfly distributions in Europe, coupled with slower population declines in southern regions. Given that the Mediterranean region is a climate change hotspot, understanding the largely unknown mechanisms employed by butterflies to cope with increasing aridity is crucial for developing data-driven conservation strategies. To address this issue, we developed a monitoring approach based on 90 transects fully covering areas of 50 m × 50 m squares instead of linear paths. This enabled us to explore the fine-scale dynamics of 80 butterfly species and their association with vegetational units in the Mediterranean hills (∼800 m a.s.l. as maximum altitude) of the MAB UNESCO Reserve of Monte Peglia (Central Italy). We sampled the transects from April to October 2022 (the locally hottest summer of the last decade) and documented three main patterns: 1) butterfly richness and abundance correlate with environmental features and these relationships vary across seasons. Richer sites consisted of some 30 % of woodland and were those closer to water, mostly during summer (area-based analysis). 2) Environmental preferences vary with species traits. Specialist species and those adapted to colder climates and narrower temperature ranges were more abundant in higher altitudes sites, closer to water and with wider woodland coverage (fourth-quadrant analysis). 3) Although most species preferred meadows, when temperature increases, they increased their abundance in sites with wider tree coverage, closer to streams, and at higher altitude (species-based analysis). These dynamics demonstrate that butterflies opportunistically move to cooler micro-habitats to buffer elevated temperatures. This phenomenon potentially involves most of the community since the species showing significant effects encompass >80 % of the observed individuals. Our analysis introduces new dimensions to the niche of Mediterranean butterflies, highlighting the significance of shady micro-habitats, altitudinal ranges, and water streams. These factors are likely to become increasingly critical with rising aridity levels. By combining our findings with existing literature data, we have provided a protocol aimed at preserving Mediterranean butterflies within the resist-accept-direct framework.

Late Cretaceous high trochospiral rotaliid foraminifera from Italy

High trochospiral architecture is relatively uncommon in rotaliid foraminifera as it is observed in a few species throughout the stratigraphic record of their 100 My history. Such a feature is recorded within the lockhartiniid group, for example in the Paleogene genus Sakesaria, whereas it is not reported from the Cretaceous. We describe two new Late Cretaceous species, Extentaspira pichezziae gen. nov., sp. nov. and Extentaspira pampaloniae sp. nov., displaying high trochospiral test, from the shallow-water facies of the Apennine and Adriatic carbonates platforms of Central and Northeastern Italy. Besides the absence of dimorphism, high trochospiral test architecture suggests an adaptation to an infaunal lifestyle. These occurrences may provide further evidence on the paleo-biogeographic gradient with the nearby Late Cretaceous Pyrenean gulf.

Groundwater and Dissolved Gases Geochemistry in the Pesaro-Urbino Province (Northern Marche, Central Italy) as a Tool for Seismic Surveillance and Sustainability

The Pesaro-Urbino province (PUP) (northern Marche, central Italy) is one of the most seismically active areas in Italy, with the most recent earthquakes (5.2 and 5.5 Mw) having occurred on 9 November 2022 with an epicenter located in the Adriatic Sea. A detailed geochemical and isotopic characterization of 87 groundwaters (and dissolved gases) circulating in the PUP was carried out to (i) unravel the geochemical processes controlling the water circulation, (ii) investigate the interplay between deep originated fluids and shallow aquifers, (iii) evaluate the reliability of specific geochemical parameters as tracers for seismic activity, and (iv) select the most suitable sampling sites to deploy a monitoring network to highlight possible compositional changes related to the regional and local tectonic activity. The geochemical dataset includes waters showing five different hydrochemical compositional facies: (i) calcium bicarbonate with low Total Dissolved Solids (TDS); (ii) calcium bicarbonate with relatively high concentrations of sulfate (>200 mg/L); (iii) sodium bicarbonate with pH > 8.8; (iv) calcium sulfate; (v) sodium chlorine. Two distinct groups of dissolved gases can be recognized: (a) N2-dominated gases with N2/Ar ratios similar to those of Air-Saturated Water (ASW); (b) CO2- and CH4-rich gases associated with high TDS and springs rich in S-bearing reduced species. The isotopic values of δ13C-CO2 and δ13C-CH4 suggest a predominant biogenic origin for both species with a negligible contribution from deep-seated fluids. The Ca-HCO3(SO4), Ca(Na)-SO4(Cl), and Na-HCO3 waters, being likely related to deep hydrological pathways, are the best candidates to be included in the monitoring network in the Pesaro-Urbino province. This will be of paramount importance in addressing the challenge of unravelling fluid geochemical precursors of earthquakes, thus increasing and improving seismic surveillance practices and hazard mitigation.