This study aimed to investigate the effect of Scolopin-2, a cationic antimicrobial peptide from centipede venoms, and amidated Scolopin-2 on Hela cell viability in vitro and in vivo. The cellular proliferation was investigated with the MTT assay. Confocal laser scanning, flow cytometry, and Western blot analysis were employed to localize Scolopin-2-NH2 in Hela cells and to study the caused cells apoptosis. We subcutaneously injected Hela cells into BALB/c nude mice and studied if Scolopin-2-NH2 suppressed tumor growth in the mice. Scolopin-2-NH2 inhibited Hela proliferation in vitro in a dose-dependent manner with an IC50 of 35 μM. In addition, Scolopin-2-NH2 combined with mitochondria and regulated caspase-related apoptosis pathways in Hela cells. Scolopin-2-NH2 significantly suppressed tumor growth in the tumor-bearing mice without side effects, such as weight loss or abnormal changes in tissues, including liver, spleen, kidney, and lung. These results indicate Scolopin-2-NH2 may be a good therapeutic candidate for the treatment of Hela cervical cancer.