Central Region Research Articles

Flow characterization and turbulence in the eastern section of the Strait of Magellan, Southern Chile

The Strait of Magellan connects the Pacific and Atlantic oceans in South America’s southern region, and it has been recognized for centuries as an important transoceanic navigation route as well as a unique marine environment with a rich ecological diversity. Evaluations of the impact of human activities in the channel and multiple potential future developments require a better understanding of the physical environment to design sustainable strategies aimed at preserving these characteristics. In this investigation, we study the flow near the Atlantic inlet of the Strait where the dynamics is characterized by the interactions of the tide propagation within two narrows, which are the predominant features of the channel morphology. Tides amplified by the Patagonian shelf generate strong currents through these narrows and control the exchange between the Atlantic and central regions of the Strait. We employ bottom-mounted and vessel-mounted Acoustic Doppler Current Profilers (ADCPs) with tide gauges to analyze the mean flow, tidal propagation, and turbulence, complementing the data with previous available measurements. The analysis reveals residual flows directed toward ebb flow at the channel center and flood near the edges, showing a significant spring-neap variation. Turbulence statistics in the second narrows exhibit a significant variability between ebb and flood, with a balance between production and dissipation observed only during ebb phases.

Enhanced immune responses are accompanied by increased MAGEA expression in osteosarcoma metastases

ObjectiveOsteosarcoma is the most common primary bone sarcoma. About 50% of patients develop metastatic disease and their 5-year survival lingers at around 20%–30%. T cell checkpoint blockade immunotherapies have revolutionised cancer treatment in the last decade, but their impact remains limited in osteosarcoma.Methods and analysisIn order to reveal potentially novel immunotherapeutic strategies for advanced osteosarcoma, we conducted an immunogenomic characterisation of a unique sample set comprising 30 osteosarcoma samples from seven patients, collected throughout disease progression. We performed RNA-sequencing and imaging mass cytometry analysis on these samples to reveal the immunological landscape during osteosarcoma progression.ResultsTranscriptional and phenotypical hallmarks of cytotoxic T cell-driven anticancer immunity were enriched in metastatic lesions as compared with primary tumours. Spatial analysis showed T cells infiltrating central regions of osteosarcoma metastases, indicating the absence of an immune excluded environment. In parallel, we found a pronounced increase in the expression of cancer testis antigens, particularly melanoma antigen family A (MAGEA)-related antigens, in osteosarcoma metastases, which was validated in an independent cohort (N=91). In addition, we demonstrated the presentation of MAGE-derived peptides in three out of four osteosarcoma cell lines.ConclusionThese findings indicate a concurrent augmentation of cytotoxic antitumour immune responses and expression of MAGEA antigens from primary to metastatic osteosarcoma. This observation suggests the exploration of MAGEA antigens as potential targets for immunotherapy in the treatment of advanced osteosarcoma.

Identifying the crucial binding domain of Histain-1 to recombinant TMEM97 in activating chemotactic migration in human corneal epithelial cells.

TMEM97, also known as the sigma-2 receptor, plays a crucial role as an endoplasmic reticular protein involved in various physiological processes such as wound healing, and cholesterol metabolism. Moreover, TMEM97 has been implicated in multiple human diseases including neurodegenerative disorders and cancers. Histatin peptides are endogenous peptides with diverse biological effects, including antimicrobial, immunomodulatory, and wound healing functions. Recent studies have revealed that histatin-1 (Hst1) acts as an endogenous ligand for TMEM97 and is essential for Hst1-induced corneal epithelial migration. In this study, we sought to establish the crucial Hst1 residues that facilitate binding to TMEM97. The purified full-length (FL)-TMEM97 expressed from Escherichia coli exhibited comparable binding affinity, as indicated by the dissociation equilibrium constant (KD) determined by Surface plasmon resonance (SPR), to commercially sourced TMEM97 expressed in mammalian cells. SPR analysis revealed that TMEM97 bound to FL-Hst1 and selected deletion mutants of Hst1. Truncation experiments pinpointed the central region of Hst1 as crucial for its binding to TMEM97, with the loss of residues 15-19 either significantly weakening or completely abolishing the binding interaction. Furthermore, alanine substitution mutant experiments highlighted residues 9-19 as critical for the interaction between TMEM97 and Hst1. Functional assays including migration and signaling were also compared for Hst1 and mutant Hst1. Collectively, these findings underscore the specific binding of Hst1 to TMEM97 and elucidate the critical regions within Hst1 necessary for this interaction which is critically important for the epithelial migration and signaling changes in the ERK and Akt pathways.

Microfluidic device-assisted 3D cell spheroids isolation, staining and embedding

3D cell spheroids have emerged as indispensable tools in cell biology research. In this study, the efficacy of an open-close switchable microfluidic device for on-chip staining and agar-embedding of 3D spheroids was thoroughly validated, especially in preparation for immunohistochemistry (IHC) analysis. The on-chip staining procedure, employing Trypan blue and Calcein-AM/PI staining, was demonstrated to evaluate the cell viability of spheroids. During overextended culture periods, a discernible decline in cell viability was observed at the central region of the spheroids, offering valuable information on the temporal aspects of spheroid development. On-chip agar embedding stands out for its precise control over the location and orientation of three-dimensional spheroids, a key factor in exploring cellular architecture, especially in fused spheroids using IHC analysis. In comparison to tube-based staining, this method significantly cuts down on reagent use and analysis time. This not only boosts efficiency but also lowers the risk of spheroid damage or loss during staining, highlighting its potential for handling miniaturized tissue samples and biopsies in cell assays. The on-chip staining and agar-embedding techniques presented here not only provide valuable insights into spheroids’ behavior but also pave the way for further developments in miniaturized tissue analysis and cell-based assays.

Spatial Distribution Pattern of Aromia bungii Within China and Its Potential Distribution Under Climate Change and Human Activity.

Aromia bungii is a pest that interferes with the health of forests and hinders the development of the fruit tree industry, and its spread is influenced by changes in abiotic factors and human activities. Therefore, exploring their spatial distribution patterns and potential distribution areas under such conditions is crucial for maintaining forest ecosystem security. This study analyzed the spatial differentiation characteristics of the geographic distribution pattern of A. bungii in China using Moran's I and the Getis-Ord General G index. Hot spot distribution areas were identified using Getis-Ord Gi*. An optimized MaxEnt model was used to predict the potential distribution areas of A. bungii within China under four shared economic pathways by combining multivariate environmental data: (1) prediction of natural environmental variables predicted under current climate models; (2) prediction of natural environmental variables + human activities under current climate models; and (3) prediction of natural environmental variables under the future climate models (2050s and 2070s). Meanwhile, MigClim was used to simulate the unoccupied suitable area in the presence of obstacles under future climate change. The results showed that human activities, minimum temperature of the coldest month, and precipitation of the wettest month had positive effects on the distribution of A. bungii. However, in the current period, human activities drastically reduced the survival area of A. bungii, and its suitable distribution area was mainly concentrated in the eastern and central regions of China. Under the influence of climate change in the future, the habitat of A. bungii will gradually increase. Additionally, the MigClim model indicates that the area unoccupied by A. bungii has been on a continuous increasing trend. This study provides a positive reference for the prevention and control of A. bungii and the maintenance of forest health and ecosystem security, and provides important theoretical guidance for researchers, policymakers, and governments.

Self-similar Buildup and Inside-out Growth: Tracing the Evolution of Intermediate-to-high-mass Star-forming Galaxies since z = 2

We aim to discern scenarios of structural evolution of intermediate-to-high-mass star-forming galaxies (SFGs) since cosmic noon by comparing their stellar mass profiles with present-day stellar masses of log(M*,0/M⊙)=10.3−11 . We addressed discrepancies in the size evolution rates of SFGs, which may be caused by variations in sample selection and methods for size measurements. To check these factors, we traced the evolution of individual galaxies by identifying their progenitors using stellar mass growth histories (SMGHs), integrating along the star-forming main sequence and from the IllustrisTNG simulations. Comparison between the structural parameters estimated from the mass- and light-based profiles shows that mass-weighted size evolves at a slower pace compared to light-based ones, highlighting the need to consider the mass-to-light ratio (M/L) gradients. Additionally, we observed mass-dependent growth in stellar mass profiles: massive galaxies ( log(M*,0/M⊙)≳10.8 ) formed central regions at z ≳ 1.5 and grew faster in outer regions, suggesting inside-out growth, while intermediate-mass and less massive SFGs followed a relatively self-similar mass buildup since z ∼ 2. Moreover, slopes of observed size evolution conflict with the predictions of TNG50 for samples selected using the same SMGHs across our redshift range. To explore the origin of this deviation, we examined changes in angular momentum (AM) retention fraction using the half-mass size evolution and employing a simple disk formation model. Assuming similar dark matter halo parameters, our calculations indicate that the AM inferred from observations halved in the past 10 Gyr while it remained relatively constant in TNG50. This higher AM in simulations may be due to the accretion of high-AM gases into disks.

Embedded Young Stellar Objects near H72.97-69.39: A Forming Super Star Cluster in N79

We present 102 embedded young stellar object (YSO) candidates associated with the H72.97-69.39 super star cluster (SSC) in the Large Magellanic Cloud (LMC). With the use of the James Webb Space Telescope Mid-Infrared Instrument (MIRI) imaging mode, we utilize an F770W – F1000W versus F1000W color–magnitude diagram to select 70 YSO candidates. An additional 27 YSO candidates are selected based on model fitting using the four MIRI imaging filters employed for this study (F770W, F1000W, F1500W, and F2100W). The central region of H72.97-69.39 is saturated in MIRI imaging, however it is covered by observations made with the Medium Resolution Spectrometer (MRS), leading to the identification of five additional massive YSOs. The total star formation rate inferred based on the 102 YSO candidates is 0.02 M ⊙ yr−1, similar to other high-mass star-forming regions in the LMC which have undergone several generations of starburst events. Due to its young age, however, H72.97-69.39's stellar production rate is expected to increase. The central five YSOs identified with MRS have masses ranging from 21.1 to 40.3 M ⊙ and total luminosity over 106 L ⊙, making H72.97-69.39 a very compact and luminous star-forming region similar to other known SSCs. We theorize that the central five massive YSOs were formed via filamentary collision, while other YSO candidates of varying masses were triggered by wind, radiation, and expanding H ii shells based on their spatial distribution.

Estimating Dust Attenuation from Galactic Spectra. III. Radial Variations of Dust Attenuation Scaling Relations in MaNGA Galaxies

We investigate the radial dependence of the scaling relations of dust attenuation in nearby galaxies using integral field spectroscopy data from MaNGA. We identify ionized gas regions of kiloparsec size from MaNGA galaxies, and for each region we estimate both the stellar attenuation E(B − V)star and gas attenuation E(B − V)gas. We then quantify the correlations of 15 regional/global properties with E(B − V)gas and E(B − V)star, using both the feature importance obtained with the Random Forest regression technique and the Spearman correlation coefficients. The importance of stellar mass, metallicity, and nebular velocity dispersion found previously from studies based on the Sloan Digital Sky Survey can be reproduced if our analysis is limited to the central region of galaxies. The scaling relations of both E(B − V)gas and E(B − V)star are found to vary strongly as one goes from the galactic center to outer regions, and from Hα-bright regions to Hα-faint regions. For E(B − V)gas, [N ii]/[S ii] is top-ranked with a much higher correlation coefficient than any other property at 0 < R ≲ R e , while [O iii]/[O ii] outperforms [N ii]/[S ii] as the leading property in the outermost region. For E(B − V)star, stellar age shows the strongest correlation with no/weak dependence on radial distance, although ΣHα and specific star formation rate present similarly strong correlations with E(B − V)star in the galactic center. We find Hα-bright regions to generally show stronger correlations with E(B − V)gas, while Hα-faint regions are more strongly correlated with E(B − V)star, although this depends on individual properties and radial distance. The implications of our results for studies of high-z galaxies are discussed.

Effect of solid loading and particle size on bubble behavior and flow field structure in slurry bubble column

The complexity of fluid dynamics in a slurry bubble column reactor introduces significant uncertainty in reactor design and scale-up. This paper investigates the hydrodynamic performance of the gas–liquid–solid system within the reactor by employing computational fluid dynamics-population balance modeling numerical simulations alongside particle image velocimetry (PIV) experiments. The effect of superficial gas velocity and particle conditions on the overall gas holdup were analyzed, focusing on the effects of particle size and solid concentration on bubble size, bubble behavior, flow field structure, and local gas holdup distribution at high superficial gas velocities. Bubble size was evaluated using calibrated image measurements, and the impact of varying solid conditions was thoroughly explored. The results revealed that an increase in solid size correlated with higher gas holdup and smaller bubble sizes, whereas a greater solid concentration resulted in decreased gas holdup and larger bubble sizes. PIV experiments indicated that bubbles exhibited a tendency to migrate toward the central region of the reactor, leading to the formation of larger bubbles that accelerated the rise of surrounding bubbles, while smaller bubbles near the wall moved downward. As the slurry bed height increased, the range of local gas holdup distribution expanded, resulting in a symmetrical distribution of radial local gas holdup in the fully developed stage at a height of 0.16 m.

A cross-sectional geospatial analysis of access to cesarean delivery in Uganda by facility functionality

Introduction: There is minimal data on spatial access to surgical maternal care in Uganda. We aimed to determine geospatial access to facilities performing cesarean delivery in Uganda at differing facility functional levels. Methods: We defined three levels of facility functionality: level 1 – functional by designation, i.e., health system level; level 2 – performed at least one cesarean delivery in the past year; and level 3 – performed at least 20 cesarean deliveries in the past year. We mapped facilities in ArcGIS Pro using GPS coordinates, calculated Manhattan distances, and using transportation network data, identified the area indicating 30-minute, 1-hour, and 2-hour geographic walking and driving access to facilities. The population estimate raster was overlain with drive time analysis areas to calculate the total population catchment within each travel time. Results: Of 6937 total facilities, 339 (4.9%) met level 1 functionality, 287 (4.1%) met level 2 functionality, and 241 (3.5%) met level 3 functionality. The percentages of women within 30-minute, 1-hour, and 2-hour walking times from level 1 facilities were 9.7%, 19.8%, and 34.8%, respectively. These percentages decreased to 9.2%, 19%, and 33% for level 2 functionality and 8.1%, 17.4%, and 30.5% for level 3 functionality, respectively. Access varied regionally, with the poorest access in the Northern region (20% within 2 hours walking) compared to the Central region (55.2% within 2 hours walking). Conclusion: We demonstrate a substantial proportion of the Ugandan population does not have timely access to cesarean delivery, with substantial subnational variation. This inequity in access to surgical obstetric care is vital information that can assist policymakers in achieving universal maternal health access.

An interferometric search for SiO maser emission in planetary nebulae

Maser emission of SiO, H$_2$O, and OH is widespread in asymptotic giant branch (AGB) stars with oxygen- (O-) rich envelopes. This emission quickly disappears during the post-AGB phase and is extremely rare in planetary nebulae (PNe). So far, only eight PNe have been confirmed to show OH and/or H$_2$O maser emission, and none have ever been found to show SiO maser emission. We intend to obtain the first detection of a SiO maser from a PN. Such a detection would provide us with a useful tool to probe mass loss in PNe at a scales of a few AU from the central star, much shorter than the scales traced by H$_2$ or OH masers. We compiled two different samples. The first one comprises all known PNe with confirmed OH and/or H$_2$O maser emission, as well as two candidate PNe showing OH masers. For the second sample, we compiled single-dish SiO maser detections in the literature, and compared them with catalogs of PNe and radio continuum emission (which could trace photoionized gas in PNe). We identified five targets (either PN or radio continuum sources) within the beam of the single-dish SiO maser observations. We then carried out interferometric observations of both samples with the Australia Telescope Compact Array, to confirm the spatial association between continuum and SiO maser emission. We find no SiO maser emission associated with any confirmed or candidate PN. In all targets, except IRAS 17390$-$3014, there is no spatial coincidence between SiO masers and radio continuum emission. While in IRAS 17390$-$3014 we cannot completely rule out a possible association, it is unlikely that the radio continuum emission arises from a PN. The absence of SiO maser emission in PNe showing OH or H$_2$O masers is of special interest, since thermal SiO emission has been reported in at least one of these targets, indicating that SiO molecules can be present in the gas phase. Since some maser-emitting PNe show evidence of having O-rich outer envelopes, and carbon- (C-) rich central stars and inner envelopes, we speculate that SiO abundance could be very low in the central regions where physical conditions are optimal for maser pumping, and C-bearing molecules may be dominant in the gas phase at those locations.

Study on the Interactive Effect Between Public Library Construction and Human Capital Investment Efficiency

This paper measures the public library construction (PLC) level and the human capital investment (HCI) efficiency of 31 provinces in China from 2011 to 2021, tests the linkage effect and coupling coordination between PLC and HCI. The results show that: 1) the PLC level and the HCI efficiency of China shows a trend of rapid increase and fluctuating decrease respectively during this period, but the gap between regions is obvious. Both the PLC level and the HCI efficiency in the eastern region are obviously better than those in the central region and the western region. 2) There is not only a positive linkage effect between PLC and HCI efficiency, which promotes each other, but also the level of collaborative development between PLC and HCI efficiency shows a steady upward trend, with the coupling level reaching level 6, which is about to enter the primary coordination state, but there are obvious regional differences.

Newly found and rediscovered hornworts (Anthocerotophyta) in Poland: Indicators of climate change impact in Central Europe.

In 2023, field research in south-western Poland led to the noteworthy discovery of two hornworts: Notothylasorbicularis, a species previously unrecorded in this country, and the rediscovery of Anthocerosneesii for the Polish bryoflora. These findings are significant as they suggest a response to climate change, which is facilitating the range expansion of hornworts within Central Europe. Detailed descriptions of the new localities for both species are provided, highlighting the specific environmental conditions and habitats where they were found. Distribution maps for Notothylasorbicularis and Anthocerosneesii in Poland are provided, as well as SEM micrographs of spores. Additionally, a key to the identification of Polish hornwort species is also included. Furthermore, a model projecting the potential future spread of these hornworts within Poland and the broader Central European region is presented. This model considers climatic variables and habitat availability, offering insights into possible range shifts. This study contributes to the growing body of evidence that climate change is a driving factor in the redistribution of bryophytes.

Ecological and biological features of the cultivation of shrubby introduced plants in the conditions of the Central Chernozem region

Ecological and biological features of the cultivation of shrubby introduced plants in the conditions of the Central Chernozem region

EEG-Characteristics of Aesthetic Perception and Evaluation of Artworks During a Museum Visit: А Neuroaesthetic Study

Twenty-eight people (30–70 years old, 12 men, 16 women, artists and non-artists) participated in a comparative neuroaesthetic study in the conditions of real-life visit to M. Vrubel mono-exhibition (Russian Museum, St. Petersburg). During the visit to the exhibition (usually lasted for around 60 min), EEG of participants was recorded. The subjects were looking at the paintings for 30 seconds to 3 min and evaluated the subjective aesthetic “attractiveness” of the paintings by a series of button presses (from 1 to 10). Were analyzed EEG spectral power in the α1 (8–10 Hz)-, α2 (10–13 Hz)-, β1 (13–18 Hz)-, β2 (13–30 Hz)-frequency bands during viewing the most famous Vrubel paintings (“Bogatyr”, “Swan Princess”, “Swan”, “Sitting Demon”, “Flying Demon”, “Pan”, etc.) and event-related EEG synchronization/desynchronization in relation to the subjective emotional and aesthetic evaluation of these paintings. Professional artists showed lower spectral power values in α1 (leads F3, C3, T4, Pz) and α2 (F3, Fz, F4, C3, Cz, C4, P3, Pz, P4)-frequency bands in frontal, central, and parietal regions. The artists were also found to have lower power values in β1,2-frequency bands in frontal (F3, F4, C3) regions and higher power values in occipital (O1, O2 – β1, β2) and posterior temporal (β2) cortical regions compared to the group of subjects with no special artistic education. Moreover, artists decision-making about the high emotional-aesthetic attractiveness of paintings was accompanied by an increase in event-related EEG synchronization for 11.5–27 Hz in frontal and central cortical areas over 580–360 ms before giving the response, compared to non-artists, whereas low emotional-aesthetic evaluation was characterized by 9–27 Hz EEG desynchronization, which started 60 ms before the giving the response and lasted up to 440 ms after it, in the posterior temporal and parietal regions. The differences in frontal cortical areas may indicate a higher engagement of the reward system during the perception of aesthetically pleasing paintings, and the differences in parietal and posterior temporal areas may indicate a continuing visual synthesis (more sustained visual attention) during the perception of subjectively less attractive paintings in artists compared to non-artists.

The High-Quality Development of the Construction Industry in the Context of the Digital Economy—Based on the Mediating Effect of Entrepreneurship

The digital economy is an important engine in leading the high-quality development of the economy, and entrepreneurship is an endogenous force propelling economic growth. These factors are essential drivers for advancing the transformation and upgrading of the construction industry and improving efficiency. This study integrates entrepreneurship into the analytical framework of industrial development and explores the primary pathways and theoretical mechanisms of the digital economy for the high-quality development of the construction industry from the dimensions of entrepreneurial innovation spirit and entrepreneurial pioneering spirit. On this basis, we empirically test the role mechanism between the digital economy, entrepreneurship, and the high-quality development of the construction industry. The results of this study indicate that (1) the digital economy significantly promotes the high-quality development of the construction industry through an improvement in technical efficiency; (2) the innovation and entrepreneurship of entrepreneurs are important mechanisms for the digital economy to promote the high-quality development of the construction industry, and at this stage, entrepreneurial spirit promotes more than the spirit of innovation; and (3) the development of the digital economy plays a greater role in enhancing low-productivity regions and the central and eastern regions, and the inclusive characteristics of the digital economy are beginning to emerge.

Spatiotemporal Distribution of Human Rabies and Identification of Predominant Risk Factors in China from 2004 to 2020.

Human rabies is a prevalent issue in China, posing a significant public health concern in the country. This study fitted the Bayesian model of separable in spatial and temporal variation and inseparable spatiotemporal variation in disease risk respectively based on Integrated Nested Laplace Approximation (INLA) to investigate the spatiotemporal characteristics of human rabies across 31 provinces in China from 2004 to 2020. It also investigated the influence of natural and socio-environmental factors on the incidence of the disease. Within the study period, a total of 26,807 cases of human rabies were reported, with the highest risk of incidence occurring in 2007, followed by a steady annual decline to the lowest risk in 2020. Guangxi Province exhibited the highest risk, while Jilin Province had the lowest, with the southern, central, and eastern regions reporting higher risks than the northern and western areas. By 2020, most provinces such as Guangxi and Guizhou had significantly reduced their relative risk (RR) of human rabies from historical highs. However, some provinces like Hunan, Henan, and Jiangsu experienced an increase in RR compared to previous years. As the annual average temperature increases, the risk of human rabies incidence in China correspondingly rises. Conversely, with increases in the annual average daily sunshine duration, per capita disposable income of urban residents, and local government healthcare expenditures, the risk of human rabies incidence declines. We conclude that the risk of human rabies in China initially increased and then decreased annually from 2004 to 2020. Future efforts should continuously increase financial investments in rabies prevention and control, focusing particularly on Hunan, Henan, Jiangsu, and provinces characterized by higher temperatures, shorter sunshine durations, and lower economic levels.

Formin-like 1β phosphorylation at S1086 is necessary for secretory polarized traffic of exosomes at the immune synapse in Jurkat T lymphocytes

We analyzed here how formin-like 1 β (FMNL1β), an actin cytoskeleton-regulatory protein, regulates microtubule-organizing center (MTOC) and multivesicular bodies (MVB) polarization and exosome secretion at an immune synapse (IS) model in a phosphorylation-dependent manner. IS formation was associated with transient recruitment of FMNL1β to the IS, which was independent of protein kinase C δ (PKCδ). Simultaneous RNA interference of all FMNL1 isoforms prevented MTOC/MVB polarization and exosome secretion, which were restored by FMNL1βWT expression. However, expression of the non-phosphorylatable mutant FMNL1βS1086A did not restore neither MTOC/MVB polarization nor exosome secretion to control levels, supporting the crucial role of S1086 phosphorylation in MTOC/MVB polarization and exosome secretion. In contrast, the phosphomimetic mutant, FMNL1βS1086D, restored MTOC/MVB polarization and exosome secretion. Conversely, FMNL1βS1086D mutant did not recover the deficient MTOC/MVB polarization occurring in PKCδ-interfered clones, indicating that S1086 FMNL1β phosphorylation alone is not sufficient for MTOC/MVB polarization and exosome secretion. FMNL1 interference inhibited the depletion of F-actin at the central region of the immune synapse (cIS), which is necessary for MTOC/MVB polarization. FMNL1βWT and FMNL1βS1086D, but not FMNL1βS1086A expression, restored F-actin depletion at the cIS. Thus, actin cytoskeleton reorganization at the IS underlies the effects of all these FMNL1β variants on polarized secretory traffic. FMNL1 was found in the IS made by primary T lymphocytes, both in T cell receptor (TCR) and chimeric antigen receptor (CAR)-evoked synapses. Taken together, these results point out a crucial role of S1086 phosphorylation in FMNL1β activation, leading to cortical actin reorganization and subsequent control of MTOC/MVB polarization and exosome secretion.

Formin-like 1β phosphorylation at S1086 is necessary for secretory polarized traffic of exosomes at the immune synapse in Jurkat T lymphocytes.

We analyzed here how formin-like 1 β (FMNL1β), an actin cytoskeleton-regulatory protein, regulates microtubule-organizing center (MTOC) and multivesicular bodies (MVB) polarization and exosome secretion at an immune synapse (IS) model in a phosphorylation-dependent manner. IS formation was associated with transient recruitment of FMNL1β to the IS, which was independent of protein kinase C δ (PKCδ). Simultaneous RNA interference of all FMNL1 isoforms prevented MTOC/MVB polarization and exosome secretion, which were restored by FMNL1βWT expression. However, expression of the non-phosphorylatable mutant FMNL1βS1086A did not restore neither MTOC/MVB polarization nor exosome secretion to control levels, supporting the crucial role of S1086 phosphorylation in MTOC/MVB polarization and exosome secretion. In contrast, the phosphomimetic mutant, FMNL1βS1086D, restored MTOC/MVB polarization and exosome secretion. Conversely, FMNL1βS1086D mutant did not recover the deficient MTOC/MVB polarization occurring in PKCδ-interfered clones, indicating that S1086 FMNL1β phosphorylation alone is not sufficient for MTOC/MVB polarization and exosome secretion. FMNL1 interference inhibited the depletion of F-actin at the central region of the immune synapse (cIS), which is necessary for MTOC/MVB polarization. FMNL1βWT and FMNL1βS1086D, but not FMNL1βS1086A expression, restored F-actin depletion at the cIS. Thus, actin cytoskeleton reorganization at the IS underlies the effects of all these FMNL1β variants on polarized secretory traffic. FMNL1 was found in the IS made by primary T lymphocytes, both in T cell receptor (TCR) and chimeric antigen receptor (CAR)-evoked synapses. Taken together, these results point out a crucial role of S1086 phosphorylation in FMNL1β activation, leading to cortical actin reorganization and subsequent control of MTOC/MVB polarization and exosome secretion.

Crustal Heterogeneity of the Bhutan Himalaya: Insights from PgQ Tomography

Abstract We present the results of a seismic investigation conducted in Bhutan using data from the Geodynamics and Seismic Structure of the Eastern Himalaya Region broadband network, focusing on variations in crustal structure and seismic attenuation properties. Through analysis of seismic data from 56 events recorded between 2013 and 2014, with magnitudes exceeding 4.5 and depths shallower than 50 km, we examined 1 Hz PgQ (Q0)-values among multiple station pairs using the two-station method. Our findings reveal significant disparities in PgQ0-values across Bhutan. The western region exhibits low PgQ0 values, indicating high seismic attenuation, whereas the central region shows medium-to-high PgQ0 values, suggesting lower attenuation. Notably, these results are consistent with the geometry of the Moho, providing valuable insights into crustal geometry and rheology. This study contributes to a deeper understanding of Bhutan’s complex crustal structure, offering insights into crustal properties and seismic attenuation mechanisms in this geologically significant region.