The fundamental period of structures is a parameter used in structure under design and for evaluating existing structures. Data-driven methods using ambient vibrations have become popular, particularly for the adjustment of empirical relationships applied to building classes. This study presents the results of a survey of ambient vibrations performed in 146 reinforced concrete buildings in the center of Quito (Ecuador). Classical functional forms giving period (T) for height (H) or number of floors (N) are derived and compared with the relationships available in the Ecuadorian seismic design provisions. We highlight variations in the empirical relationships according to soil conditions, but above all according to the date of construction and the historic seismic sequence to which the buildings have been exposed. The cumulative damage effect is finally confirmed by repeating ambient vibration measurements after the 2016 Mw 7.8 Pedernales earthquake located in the subduction zone, about 175 km from Quito. Even with such a long epicentral distance, leading to low macroseismic intensity (IEMS98 = IV), the seismic ground motion of between 0.017 and 0.081 g recorded in Quito reduced the resonant frequency of the buildings tested by between 2 and 13%. This confirms the effect of cumulative damage in reinforced concrete buildings located in seismic zones, even for weak ground motions, and the variability of empirical T/H relationships associated with damage.