Cellular Wall Research Articles

Revealing relationships between microstructure and hardening nature of additively manufactured 316L stainless steel

Relationships between microstructures and hardening nature of laser powder bed fused (L-PBF) 316 L stainless steel have been studied. Using integrated experimental efforts and calculations, the evolution of microstructure entities such as dislocation density, organization, cellular structure and recrystallization behaviors were characterized as a function of heat treatments. Furthermore, the evolution of dislocation-type, namely the geometrically necessary dislocations (GNDs) and statistically stored dislocations (SSDs), and their impacts on the hardness variation during annealing treatments for L-PBF alloy were experimentally investigated. The GND and SSD densities were statistically measured utilizing the Hough-based EBSD method and Taylor's hardening model. With the progress of recovery, the GNDs migrate from cellular walls to more energetically-favourable regions, resulting in the higher concentration of GNDs along subgrain boundaries. The SSD density decreases faster than the GND density during heat treatments, because the SSD density is more sensitive to the release of thermal distortions formed in printing. In all annealing conditions, the dislocations contribute to more than 50% of the hardness, and over 85.8% of the total dislocations are GNDs, while changes of other strengthening mechanism contributions are negligible, which draws a conclusion that the hardness of the present L-PBF alloy is governed predominantly by GNDs.

Hydroplastic foaming of graphene aerogels and artificially intelligent tactile sensors.

Direct foaming from solids is the most efficient method to fabricate porous materials. However, the ideal foaming fails to prepare aerogel of nanoparticles because the plasticity of their solids is denied by the overwhelming interface interactions. Here, we invent a hydroplastic foaming method to directly convert graphene oxide solids into aerogel bulks and microarrays, replacing the prevalent freezing method. The water intercalation plasticizes graphene oxide solids and enables direct foaming instead of catastrophic fragmentation. The bubble formation follows a general crystallization rule and allows nanometer-precision control of cellular wall thickness down to 8 nm. Bubble clustering generates hyperboloid structures with seamless basal connection and renders graphene aerogels with ultrarobust mechanical stability against extreme deformations. We exploit graphene aerogel to fabricate tactile microarray sensors with ultrasensitivity and ultrastability, achieving a high accuracy (80%) in artificially intelligent touch identification that outperforms human fingers (30%).

Dual antifungal activity against Candida albicans of copper metallic nanostructures and hierarchical copper oxide marigold-like nanostructures grown in situ in the culture medium.

This study aimed to determine in vitro activity of copper nanoparticles and copper nanowires against Candida albicans strains and to assess their effects on morphology and submicron structure. The microdilution method determined the minimal inhibitory concentration (MIC) of copper nanoparticles (CuNPs) and copper nanowires (CuNWs) against three strains of C. albicans: ATCC 10231 and two clinical strains (C and E). Effects on the morphology and ultrastructure of C. albicans strains were examined by scanning electron microscopy and transmission electron microscopy. MIC for CuNPs was 129·7µgml-1 for strain ATCC 10231, 1037·5µgml-1 for strain C and 518·8µgml-1 for strain E. MIC for CuNWs was similar for all strains tested (260·3µgml-1 ). SEM and TEM studies showed alterations in morphology, cell wall and the complete collapse of the yeast after incubation with CuNPs. In contrast, most of the yeast cells maintained their structure with an intact cell wall, and only decreased the number and size of fimbriae when C. albicans was exposed to CuNWs. CuNPs and CuNWs formed hierarchical copper oxide nanostructures growing in situ in the culture medium. Results suggest a dual mechanism for antifungal activity: (i) free Cu2+ ions act as a biocide, (ii) sharp edges of marigold-like petal nanostructures could injure the cellular wall and membrane and cause the death of the yeast. CuNPs and CuNWs inhibited the growth of the three strains of C. albicans tested. Moreover, CuNPs disrupted cell wall with leakage of the cytoplasmic content. Each concentration of the series used for the determination of the activity of CuNPs and nanowires against C. albicans formed copper oxide marigold-like nanostructures. This study suggests that CuNPs and CuNWs are good candidates for formulating new therapeutic agents for candidiasis.

On the Thermal Stability of Dislocation Cellular Structures in Additively Manufactured Austenitic Stainless Steels: Roles of Heavy Element Segregation and Stacking Fault Energy

The thermal stability of dislocation cellular structures in three additively manufactured (AM) austenitic stainless steels (SSs), 316L SS, 304L SS, and Al modified 316L SS (316L(Al)), were studied. Minor alloying elements, Mo and Al, were found affecting the stability of the cellular structures in AM austenitic SS, resulting in a stability ranking of AM 316L SS > AM 304L SS > AM 316L(Al) SS. As a result, their abilities towards recrystallization also differed. Owing to the high stacking fault energy (SFE) due to Al addition, AM 316L(Al) SS had the least stable subgrain cellular structure and exhibited the lowest recovery temperature. Although 316L SS possessed slightly higher SFE than 304L SS, the pinning effect due to Mo segregation at the cellular walls in AM 316L SS significantly enhanced its thermal stability. While the low-SFE AM 316L SS and AM 304L SS recovered their cellular structures via the equiaxed cell growth, the dislocation cellular walls in high-SFE AM 316L(Al) SS continuously vanished along a preferred direction. The fast recovery of cellular structures led to recrystallization retardation. The Hall–Petch model was found incapable of correlating cell size to strength because of the continuous weakening of cellular walls during heat treatment.

The Arabidopsis RLCK VI_A2 Kinase Controls Seedling and Plant Growth in Parallel with Gibberellin.

The plant-specific receptor-like cytoplasmic kinases (RLCKs) form a large, poorly characterized family. Members of the RLCK VI_A class of dicots have a unique characteristic: their activity is regulated by Rho-of-plants (ROP) GTPases. The biological function of one of these kinases was investigated using a T-DNA insertion mutant and RNA interference. Loss of RLCK VI_A2 function resulted in restricted cell expansion and seedling growth. Although these phenotypes could be rescued by exogenous gibberellin, the mutant did not exhibit lower levels of active gibberellins nor decreased gibberellin sensitivity. Transcriptome analysis confirmed that gibberellin is not the direct target of the kinase; its absence rather affected the metabolism and signalling of other hormones such as auxin. It is hypothesized that gibberellins and the RLCK VI_A2 kinase act in parallel to regulate cell expansion and plant growth. Gene expression studies also indicated that the kinase might have an overlapping role with the transcription factor circuit (PIF4-BZR1-ARF6) controlling skotomorphogenesis-related hypocotyl/cotyledon elongation. Furthermore, the transcriptomic changes revealed that the loss of RLCK VI_A2 function alters cellular processes that are associated with cell membranes, take place at the cell periphery or in the apoplast, and are related to cellular transport and/or cell wall reorganisation.

The effect of carbon dioxide on the resistance of cellular concrete wall blocks of high-rise buildings

The article is devoted to the influence of the carbonization process on the physical and technical properties of external wall blocks made of gas concrete used in high-rise buildings. The mechanism of the long-term carbonization effect of carbon dioxide on the chemical composition and physical and technical properties of gas concrete is considered.

Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis

Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense.

Recent Advances in Surface Nanoengineering for Biofilm Prevention and Control. Part II: Active, Combined Active and Passive, and Smart Bacteria-Responsive Antibiofilm Nanocoatings.

The second part of our review describing new achievements in the field of biofilm prevention and control, begins with a discussion of the active antibiofilm nanocoatings. We present the antibiofilm strategies based on antimicrobial agents that kill pathogens, inhibit their growth, or disrupt the molecular mechanisms of biofilm-associated increase in resistance and tolerance. These agents of various chemical structures act through a plethora of mechanisms targeting vital bacterial metabolic pathways or cellular structures like cell walls and cell membranes or interfering with the processes that underlie different stages of the biofilm life cycle. We illustrate the latter action mechanisms through inhibitors of the quorum sensing signaling pathway, inhibitors of cyclic-di-GMP signaling system, inhibitors of (p)ppGpp regulated stringent response, and disruptors of the biofilm extracellular polymeric substances matrix (EPS). Both main types of active antibiofilm surfaces, namely non-leaching or contact killing systems, which rely on the covalent immobilization of the antimicrobial agent on the surface of the coatings and drug-releasing systems in which the antimicrobial agent is physically entrapped in the bulk of the coatings, are presented, highlighting the advantages of each coating type in terms of antibacterial efficacy, biocompatibility, selective toxicity, as well as drawbacks and limitations. Developments regarding combined strategies that join in a unique platform, both passive and active elements are not omitted. In such platforms with dual functionality, passive and active strategies can be applied either simultaneously or sequentially. We especially emphasize those systems that can be reversely and repeatedly switched between the non-fouling status and the bacterial killing status, thereby allowing several bacteria-killing/surface regeneration cycles to be performed without significant loss of the initial bactericidal activity. Eventually, smart antibiofilm coatings that release their antimicrobial payload on demand, being activated by various triggers such as changes in local pH, temperature, or enzymatic triggers, are presented. Special emphasis is given to the most recent trend in the field of anti-infective surfaces, specifically smart self-defensive surfaces for which activation and switch to the bactericidal status are triggered by the pathogens themselves.

Effect of Silver Diamine Fluoride on Reducing Candida albicans Adhesion on Dentine.

Candida albicans is the most frequent pathogenic fungus in oral cavities. It adheres to dental tissues as part of dental plaques and contributes to caries formation. To evaluate the effect of silver diamine fluoride (SDF) on reducing C. albicans adhesion on dentine surfaces. Flat dentine surfaces were prepared from bovine dental disks, and samples were divided into three groups. The first and second groups were pretreated for 3min with 299mM or 2.99M SDF, respectively, and the third group (control) did not undergo any SDF pretreatment. All samples were washed, inoculated with C. albicans suspension onto their dentine surface, incubated at 30°C for 6h, and washed again to remove any nonadherent cells. The abundance of adherent cells was investigated using colorimetric and real-time polymerase chain reaction approaches. Subsequently, the morphological changes in C. albicans by pretreatment with SDF were observed under a scanning electron microscope (SEM). SDF inhibited candidal growth at concentrations as low as 2.99µM. Dentine disks pretreated with 299mM or 2.99M SDF displayed significantly fewer adhered cells as compared with the control group. Upon pretreatment with SDF, SEM images showed severe morphological changes in the cellular walls, in a dose-dependent manner, suggesting a fungicidal effect of SDF against the yeast. SDF should be considered for clinical applications aimed at inhibiting dental plaque caused by C. albicans, particularly in children and elderly individuals.

Protein Nanoparticles with Enzymatic and Antigen-Binding Activities Induce Th1 Cytokine Gene Expression

To improve protein stability and membrane permeability the protein nanoparticles (NP) were fabricated from enzymes or virus-specific antibodies by nanoprecipitation. Lysozyme NP destroyed bacterial cellular walls. RNase-based NP hydrolyzed RNA. Lipase, catalase and horseradish peroxidase activities were also registered for corresponding protein NP. NP from polyclonal antibodies against the influenza A virus and monoclonal antibodies against hemagglutinin H1, H3; proteins NS and NP could interact with their specific antigens. NP from monoclonal antibodies against the hepatitis B surface antigen bound with the antigen as shown by ELISA. The protein NP were stable in water at 4°C for several months and in the presence of blood sera or saliva for more than 5 days. The protein NP were not toxic for human cells and mice. The protein NP could penetrate and accumulate in cells in 2 hours with maximum after 2 days and subsequent gradual decline until background values. Entry of foreign protein NP into the eukaryotic cells induced cytokine gene expression. RNA of interferon (IFN) a, b and l but not IFN g were detected by reverse transcription with real time PCR. Polarization index (Th2:Th1 cytokine RNA) was near 0. Accordingly, cellular uptake of non-toxic protein NP induced Th1 polarization of immune response.

Rotating Magnetic Nanoparticle Clusters as Microdevices for Drug Delivery.

BackgroundMagnetic nanoparticles (MNPs) hold promise for enhancing delivery of therapeutic agents, either through direct binding or by functioning as miniature propellers. Fluid-filled conduits and reservoirs within the body offer avenues for MNP-enhanced drug delivery. MNP clusters can be rotated and moved across surfaces at clinically relevant distances in response to a rotating magnet. Limited data are available regarding issues affecting MNP delivery by this mechanism, such as adhesion to a cellular wall. Research reported here was initiated to better understand the fundamental principles important for successful implementation of rotational magnetic drug targeting (rMDT).MethodsTranslational movements of four different iron oxide MNPs were tested, in response to rotation (3 Hz) of a neodymium–boron–iron permanent magnet. MNP clusters moved along biomimetic channels of a custom-made acrylic tray, by surface walking. The effects of different distances and cellular coatings on MNP velocity were analyzed using videography. Dyes (as drug surrogates) and the drug etoposide were transported by rotating MNPs along channels over a 10 cm distance.ResultsMNP translational velocities could be predicted from magnetic separation times. Changes in distance or orientation from the magnet produced alterations in MNP velocities. Mean velocities of the fastest MNPs over HeLa, U251, U87, and E297 cells were 0.24 ± 0.02, 0.26 ± 0.02, 0.28 ± 0.01, and 0.18 ± 0.03 cm/sec, respectively. U138 cells showed marked MNP adherence and an 87.1% velocity reduction at 5.5 cm along the channel. Dye delivery helped visualize the effects of MNPs as microdevices for drug delivery. Dye delivery by MNP clusters was 21.7 times faster than by diffusion. MNPs successfully accelerated etoposide delivery, with retention of chemotherapeutic effect.ConclusionThe in vitro system described here facilitates side-by-side comparisons of drug delivery by rotating MNP clusters, on a human scale. Such microdevices have the potential for augmenting drug delivery in a variety of clinical settings, as proposed.

Impression materials: does water affect the performance of alginates?

Alginic acid is a polysaccharide widely present in the cellular walls of brown algae. Alginate is widely used as a mold material in dentistry, in the production of prostheses and in the production of positives for small-scale casting. It is also used in the pharmaceutical, cosmetic and food industries for various uses. The purpose of our study is to assess whether the chemical and physical characteristics of water can influence the characteristics and performance of these materials. These impression materials are often marketed as a powder to be mixed with water, but water is not included during the purchase. We have considered different articles, but unfortunately the results that speak of this topic are few and contain little information. We have therefore carried out a review of the present literature on Pubmed and Embase search engine. The same product used by two people with two different waters will have different characteristics. The possibility of knowing these effects could allow for more performing materials and above all for identical and reproducible materials. In conclusions, the results indicate to follow the manufacturer's instructions, and eventually turn to the use of automatic devices.

Athermal ω phase transformation from β in Ti-28Ta driven by ultra-rapid quenching

The athermal ω phase has been reported to be preferably formed at the quench rate below 103 K/s in Ti-30 at.%Ta alloys. Contrary to this common viewpoint, this work presents a similar compositional alloy of Ti-28 at.%Ta with the existence of athermal ω fabricated by plasma rotating electrode process upon quenching at an ultra-high rate of 104–106 K/s. This study aims to probe the underlying mechanism for the athermal ω formation after ultra-rapid quenching of the Ti-28 at.%Ta. Microstructure and phase constituent of the ultra-rapid solidified (URS) Ti-28Ta were investigated. Results showed that the cellular-structured URS Ti-28Ta exhibited predominant β with martensitic α” and athermal ω. Elemental microsegregation is resulted in the URS Ti-28Ta with Ti atoms preferred to concentrate at the cellular walls while Ta accumulate in the matrix. Cellular walls are fully concentrated with martensitic α” and dislocations but free of ω, while the dislocation-free β matrix nearby the walls contains the athermal ω. The athermal ω is suppressed by the high-density dislocations at the grain boundaries, while compositional fluctuation due to the microsegregation in the metastable β matrix favor to form the athermal ω.

The Role of Mechanoperception in Plant Cell Wall Integrity Maintenance.

The plant cell walls surrounding all plant cells are highly dynamic structures, which change their composition and organization in response to chemical and physical stimuli originating both in the environment and in plants themselves. They are intricately involved in all interactions between plants and their environment while also providing adaptive structural support during plant growth and development. A key mechanism contributing to these adaptive changes is the cell wall integrity (CWI) maintenance mechanism. It monitors and maintains the functional integrity of cell walls by initiating adaptive changes in cellular and cell wall metabolism. Despite its importance, both our understanding of its mode of action and knowledge regarding the molecular components that form it are limited. Intriguingly, the available evidence implicates mechanosensing in the mechanism. Here, we provide an overview of the knowledge available regarding the molecular mechanisms involved in and discuss how mechanoperception and signal transduction may contribute to plant CWI maintenance.

On the strengthening and embrittlement mechanisms of an additively manufactured Nickel-base superalloy

The γ′ phase strengthened Nickel-base superalloy is one of the most significant dual-phase alloy systems for high-temperature engineering applications. The tensile properties of laser powder-bed-fused IN738LC superalloy in the as-built state have been shown to have both good strength and ductility compared with its post-thermal treated state. A microstructural hierarchy composed of weak texture, sub-micron cellular structures and dislocation cellular walls was promoted in the as-built sample. After post-thermal treatment, the secondary phase γ′ precipitated with various size and fraction depending on heat treatment process. For room-temperature tensile tests, the dominated deformation mechanism is planar slip of dislocations in the as-built sample while dislocations bypassing the precipitates via Orowan looping in the γ′ strengthened samples. The extraordinary strengthening effect due to the dislocation substructure in the as-built sample provides an addition of 372 MPa in yield strength. The results of our calculation are in agreement with experimental yield strength for all the three different conditions investigated. Strikingly, the γ′ strengthened samples have higher work hardening rate than as-built sample but encounter premature failure. Experimental evidence shows that the embrittlement mechanism in the γ′ strengthened samples is caused by the high dislocation hardening of the grain interior region, which reduces the ability to accommodate further plastic strain and leads to premature intergranular cracking. On the basis of these results, the strengthening micromechanism and double-edge effect of strength and ductility of Nickel-base superalloy is discussed in detail.

Eradication of planktonic Vibrio parahaemolyticus and its sessile biofilm by curcumin-mediated photodynamic inactivation

Vibrio parahaemolyticus is the leading pathogen in seafoods. This study established the efficient blue light-emitting diode (LED) photodynamic inactivation (PDI) to eradicate Vibrio parahaemolyticus and its biofilm with photosensitizer curcumin, and the antibacterial and antibiofilm mechanisms were elucidated by determining the DNA integrity, protein changes, morphological alteration, gene expression levels, chemical composition and structural parameters of biofilm, etc. Results showed that the planktonic V. parahaemolyticus could not be visibly detectable on the medium after the curcumin-mediated PDI treatment with 1.0 μM curcumin within 5 min (1.14 J/cm2), and its biofilm was almost completely eradiated with 20.0 μM curcumin within 60 min (13.68 J/cm2). The cellular wall and proteins of V. parahaemolyticus were the vulnerable target for the PDI treatment. Meanwhile, the PDI efficiently inactivated the living cells, reduced the key chemical composition of extracellular polymeric substance and negatively altered the architectures of biofilm. Furthermore, the PDI treatment down-regulated the expression of virulence genes (tdh and toxR) and biofilm formation genes (oxyR, aphA, luxR and opaR) of V. parahaemolyticus, which would impede the bacterial infection, colonization and biofilm formation. The study will enrich our knowledge of the PDI-induced inactivation of V. parahaemolyticus, hence unlock the design of novel PDI technology to eradicate bacteria in food industry.

Evaluation of citrus flavonoids against Aspergillus parasiticus in maize: Aflatoxins reduction and ultrastructure alterations

In this study, the effects of citrus flavonoids naringin (NAR), neohesperidin (NEO) and quercetin (QUER) on aflatoxins accumulation by a selected Aspergillus parasiticus strain in maize at 0.95 aw were studied by response surface methodology using a Box-Behnken design. Multiple response optimization was applied to simultaneously minimize the contamination with aflatoxins (AFs) B1, G1, B2 and G2. The application of the optimal mixture in maize at 0.95 aw (0.39 mM NAR, 0.24 mM NEO and 0.40 mM QUER) reduced from 85% to 100% AFs accumulation. The same mixture at 0.98 aw, led to a reduction in AFs accumulation that ranged from 93% to 98%.Ultrastructure alterations of cellular membranes and walls in A. parasiticus, evidenced by transmission electron microscopy images, were severe and depended on the type of flavonoid and their combination. Flavonoid mixtures may provide an environmentally friendly alternative for decreasing AFs accumulation in stored maize, replacing synthetic compounds.

A Review on Staphylococcus sp. and its pathogens

The genus Staphylococcus is an important microorganism because of its direct and dangerous impact on the lives of other organisms, and Alexander Ogaston was the first to release this designation in 1880 to denote spherical bacteria usually associated with pus in wound infections. The members of this species are found in various environments such as dust, water, air, feces, on the skin, and the mucous membranes of warm-blooded vertebrates, on clothing, and other places. Most species of this species live as natural flora on human skin and mucous membranes, but some are opportunistic pathologies. The species of staphylococcus is characterized by having several virulence factors that increase the efficacy and pathogenicity of its species on the occurrence of infection due to its possession of tequic acid and the adhesion factor and other factors that enable it to get rid of the host defenses as an enzyme (Staphylokinase) or enable it to invade and spread as an enzyme (Hyaluronidase). Its possession of cellular wall proteins, its formation of the capsule and the exogenous polysaccharides and its production of various types of extracellular enzymes and various toxins such as intestinal toxins fixed in the heat, secretion of immune system inhibitors and their containment of antibiotic resistance genes. It shows the total resistance to the degradation of lysozyme. Staphylococcus aureus is resistant to many antimicrobial agents and thus produces treatment problems. The sex of staphylococcus includes 36 species and 80 subtypes, where more is clarified From 20 types of staphylococcus aureus.

Selectivity of Bedaquiline reacting with different polypeptide chains. Theoretical approach

ABSTRACTIn this article, we take a theoretical approach to analyse the pharmaceutical activity of Bedaquiline. This pharmaceutical agent has proved to be efficacious for tuberculosis treatment; however, it also shows toxic effects for human beings. Therefore, by simulating (DFT-ADMP) reactions, this study has reproduced the chemical activity of this substance, when faced with fragments of polypeptides coming from different sources; for example, the cellular wall of the Koch basilum or the corresponding chain from human albumin cells. Bedaquiline's preference for glutamate and arginine is demonstrated on the basis of chemical arguments and we propose the design of a Fullerene-Bedaquiline complex with low toxic effects.

Effects of cell aspect ratio and relative density on deformation response and failure of honeycomb core structure

The extensive applications of honeycomb (HC) core in sandwich structures necessitates the influence of the cellular geometry and cell wall base material on the mechanical response to be quantified. In this respect, this paper establishes the mechanics of the deformation and the failure processes of the HC core under the out-of-plane compressive, tensile, and shear loading. The corresponding mechanical properties are determined and the mechanisms of failure of the HC core structure are identified. The influence of the relative density (ρ*/ρs) and the cell aspect ratio (H/c) of the hexagonal HC core on the compressive deformation response, the out-of-plane properties and the characteristic dissipation energy density (DED) of the structure is established. Results show that the compressive strength increases exponentially from 1.5 to 10.6 MPa over the relative density range of 0.028 ≤ (ρ*/ρs) ≤ 0.125. The out-of-plane shear modulus, G13 and G23 are 33.9 and 58.2 MPa, while the shear strength, τ13 and τ23 are 1.07 and 2.03 MPa, respectively. The HC core with a low aspect ratio (H/c < 2.64) failed due to the early debonding of the double-wall hexagonal cells, while at H/c ≥ 2.64, by elastic buckling of the cells. A phenomenological model is formulated to highlight the combined effects of both parameters on the compressive strength (σc) of the HC cores, covering the range of 0.028 ≤ (ρ*/ρs) ≤ 0.056 and 2.5 ≤ (H/c) ≤ 5.62. Furthermore, the characteristic dissipation energy density (DED) under the out-of-plane compression varies linearly within the range of 2.5 < (H/c) < 5.62 for the HC core with ρ*/ρs = 0.056. The HC core with H/c = 3.96, but with twice higher ρ*/ρs exhibits about twice larger DED. These resulting properties and failure mechanisms of the anisotropic paper-based HC core are useful for the validation of the predictive computational models.