Cellular Space Research Articles

The impact of biomembranes and their dynamics on organismic aging: insights from a fungal aging model.

Biomembranes fulfill several essential functions. They delimitate cells and control the exchange of compounds between cells and the environment. They generate specialized cellular reaction spaces, house functional units such as the respiratory chain (RC), and are involved in content trafficking. Biomembranes are dynamic and able to adjust their properties to changing conditions and requirements. An example is the inner mitochondrial membrane (IMM), which houses the RC involved in the formation of adenosine triphosphate (ATP) and the superoxide anion as a reactive oxygen species (ROS). The IMM forms a characteristic ultrastructure that can adapt to changing physiological situations. In the fungal aging model Podospora anserina, characteristic age-related changes of the mitochondrial ultrastructure occur. More recently, the impact of membranes on aging was extended to membranes involved in autophagy, an important pathway involved in cellular quality control (QC). Moreover, the effect of oleic acid on the lifespan was linked to basic biochemical processes and the function of membranes, providing perspectives for the elucidation of the mechanistic effects of this nutritional component, which positively affects human health and aging.

Phosphate in Cardiovascular Disease: From New Insights Into Molecular Mechanisms to Clinical Implications.

Hyperphosphatemia is a common feature in patients with impaired kidney function and is associated with increased risk of cardiovascular disease. This phenomenon extends to the general population, whereby elevations of serum phosphate within the normal range increase risk; however, the mechanism by which this occurs is multifaceted, and many aspects are poorly understood. Less than 1% of total body phosphate is found in the circulation and extracellular space, and its regulation involves multiple organ cross talk and hormones to coordinate absorption from the small intestine and excretion by the kidneys. For phosphate to be regulated, it must be sensed. While mostly enigmatic, various phosphate sensors have been elucidated in recent years. Phosphate in the circulation can be buffered, either through regulated exchange between extracellular and cellular spaces or through chelation by circulating proteins (ie, fetuin-A) to form calciprotein particles, which in themselves serve a function for bulk mineral transport and signaling. Either through direct signaling or through mediators like hormones, calciprotein particles, or calcifying extracellular vesicles, phosphate can induce various cardiovascular disease pathologies: most notably, ectopic cardiovascular calcification but also left ventricular hypertrophy, as well as bone and kidney diseases, which then propagate phosphate dysregulation further. Therapies targeting phosphate have mostly focused on intestinal binding, of which appreciation and understanding of paracellular transport has greatly advanced the field. However, pharmacotherapies that target cardiovascular consequences of phosphate directly, such as vascular calcification, are still an area of great unmet medical need.

Modeling and analysis of spatial difference and evolution of agricultural parks based on topological segmentation and cluster analysis

When the traditional evolution model studies the regional distribution of agricultural parks, the relationship between regions is not clear enough, which leads to the lack of generality of regional distribution. In order to solve this problem, this study adopts the methods of topological division and cluster analysis, establishes the model of regional diversity and evolution of farms, and clusters the spatial information of agricultural parks. According to the feature factors, the images are classified, and the image dimension values are reduced. The data space is divided into cellular space by the method of network topology structure division, and the effective coefficient of each cell is calculated, and the spatial structure and characteristics of agricultural parks are extracted to reveal the similarities and differences between different parks. The experimental results show that the evolution model of agricultural parks constructed by topological division and clustering method shows obvious clustering characteristics in space, and the relationships among the factors are good. It is proved that the model can describe the spatial differences and evolution trends of agricultural parks more accurately, so as to provide more targeted suggestions for the planning, management and sustainable development of agricultural parks.

Clinical and anatomical rationale for the effectiveness of treatment of phlegmon of the floor of the oral cavity and deep cellular spaces of the neck complicated with sepsis

Clinical and anatomical rationale for the effectiveness of treatment of phlegmon of the floor of the oral cavity and deep cellular spaces of the neck complicated with sepsis

The normal sonographic picture of the paratonsillar region and the prerequisites for ultrasound diagnosis of purulent pathology of the pharynx

Paratonsillar abscess (PTA) stands out among all otorhinolaryngological diseases, since it can cause life-threatening complications that develop in the shortest possible time due to the close connection of the cellular spaces of the head, neck and mediastinum. In addition to physical examination, computed tomography and magnetic resonance imaging are routinely used to diagnose PTA, sonography is less often used. With the help of the latter, it is possible to reliably distinguish PTA from both paratonsillitis and a number of other diseases, including an aneurysm of the internal carotid artery, using the Doppler mode. However, there are significant gaps in the ultrasound diagnosis of PTA and paratonsillitis: the use of a percutaneous convex sensor is not described, there is no description of clear anatomical landmarks for reliable and rapid recognition of PTA, and diagnostically complete algorithms for verification and visualization of paratonsillitis and PTA using sonographic examination have not been developed. Our work is aimed at filling these gaps.

Histological Evaluation of Arthritis in Mice

Arthritis is one the almost commonly prevalent painful diseases which cause limit the movement of joints. This study conducts to investigate histological alterations resulted in rheumatoid arthritis (RA)-induced in mice and estimating the levels of some serum biomarkers including serum IL-6, CRP and RF. Totally, 30 were purchased, acclimated, and divided into two groups; control that injected distilled water, and arthritis that injected adjuvant (CFA). Post the 25th day of experiment, all study mice of both groups were subjected for collection of blood and right ankle joints. Serologically, the findings of IL-6, CRP and RF of arthritis group were elevated significantly when compare to control mice. Histologically, the findings observed in mice of control group were included the proliferation of chondrocytes of articular cartilage with moderate cellular and subchondral marrow space, infiltration few inflammatory cells in dermis, proliferation of chondrocytes in articular cartilage with absence of inflammatory reaction in synovial cavity, moderate infiltration of inflammatory cells in subcutaneous tissue, nearly normal articular cartilage with regular tidemark and absence of sclerosis in marrow spaces, regenerative articular cartilage and regular tidemark line with presence of very narrow distance between articular cartilage and marrow bone, and existence of normal bone trabeculae. In arthritis mice, there were an infiltration of inflammatory cells in subcutaneous tissues and dermis, marked increases in numbers of osteoclasts, sclerosis, regular tidemark and proliferation of chondrocytes, low amonunt of abscesses in subcutaneous tissue, thickness of bone trabeculae, fragments of bone, subchondral sclerosis, moderate distance between bone and articular cartilage. In conclussion, RA causes severe impacts on tissues of affected joints. Studying the mechanism of inhibition during different inflammatory process appear needed.

Laser resolidification of Al-5Mg-0.1Sc alloy: Growth of cells and banded structures

The versatility of Al-Mg alloys makes them well-suited for applications ranging from everyday items to cutting-edge fields like automotive, microelectronics, and aerospace. Moreover, although Al-Mg alloys hold promise for improved surface features in components, research on their laser surface remelting (LSR) remains scarce, presenting an open opportunity to create novel components with these materials. Understanding the growth modes and morphologies of laser surface remelted products is critical in this context. Using a laser instrument, optical microscopy, SEM, XRD, Vickers hardness and image analysis techniques, a correlative analysis of microstructure features and laser heat input is proposed in this research. Furthermore, the Al-5Mg-0.1Sc (wt%) alloy was remelted using two distinct original substrates, each exhibiting varying degrees of microstructure coarsening. Additionally, four distinct laser heat input conditions were applied to each case. All molten pools regions were formed either by cells or bands, with higher growth rates producing a higher fraction of bands. While the variation in the original microstructure of the substrate did not significantly impact both cellular spacings and band/cellular fractions, the microhardness varied remarkably with the reduction in energy input from 10.0 J/mm to 1.7 J/mm. Kurz and Fisher model provided an accurate prediction of the cell spacings formed at the laser molten pool.

The diagonal of cellular spaces and effective algebro-homotopical constructions

In this survey article we discuss certain homotopy coherent enhancements of the coalgebra structure on cellular chains defined by an approximation to the diagonal. Over the rational numbers, C_\infty -coalgebra structures control the \mathbb{Q} -complete homotopy theory of spaces, and over the integers, E_\infty -coalgebras provide an appropriate setting to model the full homotopy category. Effective constructions of these structures, the focus of this work, carry geometric and combinatorial information which has found applications in various fields including deformation theory, higher category theory, and condensed matter physics.

Mathematical modeling method based on heterogeneous cellular network algorithm and computer multi-dimensional space

Due to the rapid development of services such as computer multi-dimensional space, traditional cellular networks are quantified and diversified, and it is difficult to meet the future connection needs of green network users. The heterogeneous cellular network algorithm places multiple microcells and relay nodes under the macrocell. This multi-layer network architecture can not only shorten the data transmission link, but also reduce the energy consumption of base stations and terminals. In addition, through spectrum space reuse Capability and spectrum efficiency improve the efficiency of the system, but this also increases the number of base stations and network construction costs, and significantly increases energy consumption and operating costs of operators' electricity expenditures. In addition, in order to achieve parallel transmission of data, placing multiple millimeter wave input and output antenna systems in heterogeneous network microcells can meet the high-speed data needs of indoor users, but the antenna array requires many RF links to drive, which increases the hardware implementation. Complexity, cost and energy consumption. According to the above-mentioned problems, a reconfigurable modular robot is established based on the multi-dimensional computer space. It is composed of modules with the same interface and can be assembled into different configurations according to different tasks. Compared with the original robot, the reconfigurable modular robot is more adaptable to the work and environment, and flexible. Then the basic and core technologies such as kinematics, type synthesis, scale synthesis, error and control of the reconfigurable modular robot are mathematically modeled, so as to effectively apply and promote the reconfigurable modular robot in practice, and make it has theoretical and practical value.

Solidification, α-Al morphologies, and tensile properties of low-Sc modified Al-Mg alloys

Al alloys are very attractive in engineering applications that require a combination of low density and mechanical strength, such as in aeronautical and aerospace industry devices. Therefore, the search for alloys with higher specific strength is justified from environmental and economic points of view. This work aims to study the influence of cooling rates on the phase morphology and tensile properties of ternary Al-Mg-Sc alloys, whose composition covers the spectrum of low-Sc Al-Mg-Sc alloys. The ternary Al-3, −5, and − 10 wt% Mg-0.1 wt% Sc alloys were processed by transient directional solidification. This process induces a wide range of cooling rates which causes the formation of a microstructural variety along the longitudinal cooling direction and, consequently, different tensile properties. Several characterization methods were employed, including: thermal analysis, x-ray diffraction (XRD) structural analysis, SEM-EDS, optical microscopy, CALPHAD, tensile tests, and fractography. Two major findings should be noted after fully characterizing the microstructures, the formed phases, and the tensile properties. Solidification evolution revealed that more Mg added resulted in higher solidification rates due to higher fluidity with more Mg. Furthermore, cell growth was mapped in the three alloy compositions while competing with dendrites. Solidification scaling relationships between dendritic or cellular spacing and cooling rate for all alloys were examined. Although the Al-10 wt% Mg-Sc alloy had more Mg in solid solution, the Al-5 wt% Mg-Sc alloy showed superior tensile strength due to the predominance of cells and thinner primary dendrites trunks forming its microstructure.

An experimental investigation of the effects of radiation and thermal parameters on the aluminum 1050 obtained from upward directional solidification

During the reactor operation, aluminum tank and the core components can experience radiation damage, resulting in limitation of its life span. Effects of radiation on the aluminum alloys are not yet fully understood, especially when material is obtained from upward directional solidification under transient heat-flow condition. For this paper, an experimental research has been conducted to investigate the radiation effects on the as-cast aluminum 1050, with focus on the microstructure, microhardness and porosity for different position along casting. Firstly, thermal parameters were determined during solidification experiment and then correlated with cellular spacings, microhardness and porosity content. The experimental results show that thermal parameters such as solidification speed, thermal gradient and cooling rate exerts an influence on the microstructure, microhardness and porosity. Experimental laws for microstructure, microhardness and porosity have been determined, indicating that the increase in thermal parameters has induced a refinement effect on the microstructure, increase in microhardness and a decrease in the porosity content. Further, measurable effects of the gamma radiation on the microstructure, microhardness and porosity formation along the ingot of aluminum 1050, were experimentally determined. It stands out among results that gamma radiation has favored an increase in cellular spacings, microhardness and porosity in ingot, obtained from a vertical upward directional solidification. However, even after the gamma radiation exposure, microstructure, microhardness and porosity found along the casting are dependent of the solidification thermal conditions.

Negative pressure wound therapy in the treatment of purulent wounds of the maxillofacial superficial cellular spaces

Relevance. The choice of the method for purulent wound management (open or closed) is a relevant problem in surgery. The way of drainage is the fundamental factor of the effect on the wound. The open-drainage systems are most frequent in maxillofacial surgery, and the drain is passive, combined with various drug administration. It has significant drawbacks: the short action of the injected substances, painful dressing changes, long wound healing time, etc. The instillation drainage therapy is not widely available and is used only in clinics with appropriate equipment. Active draining (negative pressure suction drainage system) allows closed wound management. Though, it is still rarely used in maxillofacial surgery.Purpose. The study aimed to increase the effectiveness of the treatment of maxillofacial purulent wounds formed after the incision of odontogenic soft tissue purulent inflammatory processes using negative pressure wound therapy.Material and methods. The study was a comparative analysis of clinical and laboratory indicators of 303 patients with purulent wounds formed after incision of superficial cellular spaces’ abscesses and phlegmons. The patients formed two groups: the main group had negative pressure wound therapy (NPWT), and the comparison group had wound treatment with the traditional open method. NPWT methodology included the tubular drain placement into the purulent cavity formed after the incision and administration of a surgical film on the wound. The distal tube end was attached to a vacuum source that simultaneously was a canister for exudate collection and analysis. We followed up on clinical manifestations and endointoxication indicators and studied the wound content toxicity and the dynamics of the respiratory enzyme succinate dehydrogenase activity.Results. The negative pressure system appears to allow fast and atraumatic wound management, reducing the inflammatory stage of the wound healing process, promoting quick normalization of endointoxication parameters, which improves the patient’s general condition (fast resolution of the clinical signs), and the effect of vacuum-assisted closure allows avoiding secondary wound closure.Conclusion. Low-dose negative pressure provides the wound healing process with specific characteristics. Continuous exudate aspiration promotes rapid elimination of bacteria and detoxification of the wound and surrounding tissues, normalization of endointoxication parameters, provides anti-oedematous and analgesic effects, early restoration of impaired functions, and the time of wound healing process reaches that of healing by primary tension, which allows us to reduce the number of medications, as well as treatment and rehabilitation time.

Mechanistic constraints on the trade-off between photosynthesis and respiration in response to warming.

Phytoplankton are responsible for half of all oxygen production and drive the ocean carbon cycle. Metabolic theory predicts that increasing global temperatures will cause phytoplankton to become more heterotrophic and smaller. Here, we uncover the metabolic trade-offs between cellular space, energy, and stress management driving phytoplankton thermal acclimation and how these might be overcome through evolutionary adaptation. We show that the observed relationships between traits such as chlorophyll, lipid content, C:N, and size can be predicted on the basis of the metabolic demands of the cell, the thermal dependency of transporters, and changes in membrane lipids. We suggest that many of the observed relationships are not fixed physiological constraints but rather can be altered through adaptation. For example, the evolution of lipid metabolism can favor larger cells with higher lipid content to mitigate oxidative stress. These results have implications for rates of carbon sequestration and export in a warmer ocean.

Depicting a cellular space occupied by condensates.

Condensates have emerged as a new way to understand how cells are organized, and have been invoked to play crucial roles in essentially all cellular processes. In this view, the cell is occupied by numerous assemblies, each composed of member proteins and nucleic acids that preferentially interact with each other. However, available visual representations of condensates fail to communicate the growing body of knowledge about how condensates form and function. The resulting focus on only a subset of the potential implications of condensates can skew interpretations of results and hinder the generation of new hypotheses. Here we summarize the discussion from a workshop that brought together cell biologists, visualization and computation specialists, and other experts who specialize in thinking about space and ways to represent it. We place the recent advances in condensate research in a historical perspective that describes evolving views of the cell; highlight different attributes of condensates that are not well-served by current visual conventions; and survey potential approaches to overcome these challenges. An important theme of these discussions is that the new understanding on the roles of condensates exposes broader challenges in visual representations that apply to cell biological research more generally.

Ticlopidine induces embryonic development toxicity and hepatotoxicity in zebrafish by upregulating the oxidative stress signaling pathway

Ticlopidine exerts its anti-platelet effects mainly by antagonizing platelet p2y12 receptors. Previously, a few studies have shown that ticlopidine can induce liver injury, but the exact mechanism of hepatotoxicity remains unclear. Oxidative stress, metabolic disorders, hepatocyte apoptosis, lipid peroxidation, and inflammatory responses can all lead to hepatic liver damage, which can cause hepatotoxicity. In this study, in order to deeply explore the potential molecular mechanisms of ticlopidine -induced hepatotoxicity, we used zebrafish as a model organism to comprehensively evaluate the hepatotoxicity of ticlopidine and its associated mechanism. Three days post-fertilization, zebrafish larvae were exposed to varying concentrations (1.5, 1.75 and 2 μg/mL) of ticlopidine for 72 h, in contrast, adult zebrafish were exposed exposure to 4 μg/mL of ticlopidine for 28 days. Ticlopidine-exposed zebrafish larvae showed changes in liver morphology, shortened body length, and delayed development of the swim bladder development. Liver tissues of ticlopidine-exposed zebrafish larvae and adults stained with Hematoxylin & Eosin revealed vacuolization and increased cellular interstitial spaces in liver tissues. Furthermore, using Oil Red O and periodic acid-Schiff staining methods and evaluating different metabolic enzymes of ticlopidine-exposed zebrafish larvae and adults suggested abnormal liver metabolism and liver injury in both ticlopidine-exposed zebrafish larvae and adults. Ticlopidine also significantly elevated inflammation and oxidative stress and reduced hepatocyte proliferation. During the rescue intervention using N-acetylcysteine, we observed significant improvement in ticlopidine-induced morphological changes in the liver, shortened body length, delayed swim bladder development, and proliferation of liver tissues showed significant improvement. In conclusion, ticlopidine might inhibit normal development and liver proliferation in zebrafish by upregulation of oxidative stress levels, thus leading to embryonic developmental toxicity and hepatotoxicity. In this study, we used zebrafish as a model organism to elucidate the developmental toxicity and hepatotoxicity induced by ticlopidine upregulation of oxidative stress signaling pathway in zebrafish, providing a theoretical basis for clinical application.

Cellular state landscape and herpes simplex virus type 1 infection progression are connected

Prediction, prevention and treatment of virus infections require understanding of cell-to-cell variability that leads to heterogenous disease outcomes, but the source of this heterogeneity has yet to be clarified. To study the multimodal response of single human cells to herpes simplex virus type 1 (HSV-1) infection, we mapped high-dimensional viral and cellular state spaces throughout the infection using multiplexed imaging and quantitative single-cell measurements of viral and cellular mRNAs and proteins. Here we show that the high-dimensional cellular state scape can predict heterogenous infections, and cells move through the cellular state landscape according to infection progression. Spatial information reveals that infection changes the cellular state of both infected cells and of their neighbors. The multiplexed imaging of HSV-1-induced cellular modifications links infection progression to changes in signaling responses, transcriptional activity, and processing bodies. Our data show that multiplexed quantification of responses at the single-cell level, across thousands of cells helps predict infections and identify new targets for antivirals.

Accurate characterization of full-chain infrared multispectral imaging features under an aerodynamic thermal environment.

The detection performance of infrared imaging systems during high-speed flight is significantly impacted by aero-optical and aero-thermal radiation effects. However, traditional numerical calculations struggle to balance accuracy and efficiency, and there is a lack of a comprehensive model for infrared imaging in an aerodynamic thermal environment. In this study, we propose a calculation method based on Cellular Automata (CA) ray tracing, which allows for parallel calculation of aero-optical and aero-thermal radiation effects by combining optical field transport rules with the cellular space obtained by interpolation under fluid-solid boundary constraints. Using this method, we extend the traditional imaging feature prediction model of the infrared imaging system to obtain an accurate characterization model of the full-chain imaging features adapted to the aerodynamic thermal environment. Finally, we investigate the characteristics of infrared multispectral imaging system in various spectral bands under the influence of aero-optical and aero-thermal radiation effects. With this full-chain imaging model, the key elements of the imaging system under aerodynamic thermal environment can be globally optimized.

Macropinoscope: Real-Time Simultaneous Tracking of pH and Cathepsin B Activity in Individual Macropinosomes.

A fluorescent sensor that allows simultaneous analysis of environmental factors in a limited cellular space is useful for understanding precise molecular interactions in live cells and their biological responses. Macropinocytosis is a ubiquitous endocytic pathway for massive uptake of extracellular fluids, resulting in the formation of macropinosomes. Although macropinocytosis may impact intracellular delivery and cancer proliferation, information on the intracellular behaviors of macropinosomes is limited. Here, we aimed to develop a macropinoscope, a sensor that simultaneously detects pH and cathepsin B activity in individual macropinosomes. A macropinosome-specific marker, dextran (70 kDa), was employed as a platform, onto which fluorescein, Oregon Green, and tetramethylrhodamine were loaded for ratiometric pH sensing and imaging. A cathepsin-B-cleavable peptide sequence bearing sulfo-Cy5 and the quencher BHQ-3 was also mounted; cleavage of the sequence was detected as an increase in sulfo-Cy5 fluorescence. A steep decrease in pH was observed 5-10 min after macropinosome formation, which was accompanied by an immediate increase in cathepsin B activity. Our design concept will lead to the development of other macropinoscopes for the simultaneous detection of other parameters in individual macropinosomes.

Optimization of desert lake information extraction from remote sensing images using cellular automata

Desert lakes are important wetland resources in the blown-sand area of western China and play a significant role in maintaining the regional ecological environment. However, large-scale coal mining in recent years has considerably impacted the deposition condition of several lakes. Rapid and accurate extraction of lake information based on satellite images is crucial for developing protective measures against desertification. However, the spatial resolution of these images often leads to mixed pixels near water boundaries, affecting extraction precision. Traditional pixel unmixing methods mainly obtain water coverage information in a mixed pixel, making it difficult to accurately describe the spatial distribution. In this paper, the cellular automata (CA) model was adopted in order to realize lake information extraction at a sub-pixel level. A mining area in Shenmu City, Shaanxi Province, China is selected as the research region, using the image of Sentinel-2 as the data source and the high spatial resolution UAV image as the reference. First, water coverage of mixed pixels in the Sentinel-2 image was calculated with the dimidiate pixel model and the fully constrained least squares (FCLS) method. Second, the mixed pixels were subdivided to form the cellular space at a sub-pixel level and the transition rules are constructed based on the water coverage information and spatial correlation. Lastly, the process was implemented using Python and IDL, with the ArcGIS and ENVI software being used for validation. The experiments show that the CA model can improve the sub-pixel positioning accuracy for lake bodies in mixed pixel image and improve classification accuracy. The FCLS-CA model has a higher accuracy and is able to identify most water bodies in the study area, and is therefore suitable for desert lake monitoring in mining areas.

Correlation between micro-to-macro mechanical properties and processing parameters on additive manufactured 18Ni-300 maraging steels

The 18-Ni 300 maraging steels with bulk cuboidal shape were fabricated by selective laser melting (SLM) to understand correlation between micro-to-macro mechanical properties and processing parameters. The as-built specimens fabricated under various laser power and scanning rate conditions were composed of α martensite phase with cellular arm spacing of 0.2 – 0.5 µm. The relative density of as-built specimens increased from 89.14% to 99.86%, depending on volume metric energy density (VED), laser power, and scanning rate, but it is more affected by the VED and laser power than the scanning rate. For porous specimens fabricated under lower VED than 73 J/mm3, the laser power had a greater impact on relative density than VED, whereas relative density of specimens had a positive correlation with VED in the range of 75 − 93 J/mm3 and reached full density. Micro Vickers hardness tended to decrease with increasing VED and relative density in porous specimens, whereas in the full dense specimens, micro Vickers hardness gradually increased with increasing VED. The contrast in micro hardness between porous and dense specimens was attributed to nanoprecipitation affected by intrinsic heat treatment during SLM. Compression test demonstrated that yield strength strongly correlated with relative density, gradually increasing to 1.13 GPa as the specimens reached full density. From the investigation, this study provided an optimized SLM processing condition for high strength as-built 18Ni-300 maraging steel.