Cellular Metal Research Articles

Selenium alleviates cadmium and copper toxicity in Gracilaria lemaneiformis (rhodophyta) with contrasting detoxification strategies

Selenium (Se) is a beneficial element for plants, and can be used to mitigate the toxicity of heavy metals. However, the detoxification of Se in macroalgae, a crucial part of aquatic ecosystem productivity, has rarely been reported. In the present study, a red macroalga Gracilaria lemaneiformis was exposed to non-essential metal cadmium (Cd) or essential metal copper (Cu) with addition of different levels of Se. We then examined the changes in growth rate, metal accumulation, metal uptake rate, subcellular distribution, as well as thiol compound induction in this alga. Se addition alleviated Cd/Cu-induced stress in G. lemaneiformis by regulating cellular metal accumulation and intracellular detoxification. Specifically, supplementation of low-level Se displayed a significant decrease in Cd accumulation, and thus alleviated the growth inhibition induced by Cd. This may be caused by the inhibitory effect of endogenous Se instead of exogenous Se on Cd uptake. Although Se addition increased bioaccumulation of Cu in G. lemaneiformis, the important intracellular metal chelators, phytochelatins (PCs), were massively induced to alleviate Cu-induced growth inhibition. High-dose Se addition did not deteriorate but failed to normalize the growth of algae under metal stress conditions. Reduction in Cd accumulation or induction of PCs by Cu could not suppress the toxicity of Se above safe levels. Se addition also altered metal subcellular distribution in G. lemaneiformis, which might affect the subsequent metal trophic transfer. Our results demonstrated that the detoxification strategies of Se between Cd and Cu were different in macroalgae. Elucidating the protective mechanisms of Se against metal stress may help us better apply Se to regulate metal accumulation, toxicity, and transfer in aquatic environment.

Duodenal macrophages control dietary iron absorption via local degradation of transferrin

AbstractIron is an essential cellular metal that is important for many physiological functions including erythropoiesis and host defense. It is absorbed from the diet in the duodenum and loaded onto transferrin (Tf), the main iron transport protein. Inefficient dietary iron uptake promotes many diseases, but mechanisms regulating iron absorption remain poorly understood. By assessing mice that harbor a macrophage-specific deletion of the tuberous sclerosis complex 2 (Tsc2), a negative regulator of mechanistic target of rapamycin complex 1 (mTORC1), we found that these mice possessed various defects in iron metabolism, including defective steady-state erythropoiesis and a reduced saturation of Tf with iron. This iron deficiency phenotype was associated with an iron import block from the duodenal epithelial cells into the circulation. Activation of mTORC1 in villous duodenal CD68+ macrophages induced serine protease expression and promoted local degradation of Tf, whereas the depletion of macrophages in mice increased Tf levels. Inhibition of mTORC1 with everolimus or serine protease activity with nafamostat restored Tf levels and Tf saturation in the Tsc2-deficient mice. Physiologically, Tf levels were regulated in the duodenum during the prandial process and Citrobacter rodentium infection. These data suggest that duodenal macrophages determine iron transfer to the circulation by controlling Tf availability in the lamina propria villi.

MicroRNA let-7d attenuates hypertrophic scar fibrosis through modulation of iron metabolism by reducing DMT1 expression.

Hypertrophic scar is an unavoidable result of wound healing following burns and trauma, which remains a challenging problem for clinicians. Previously, we demonstrated that exosomal microRNAs (miRs) of human amniotic epithelial cells accelerated wound healing and inhibited scar formation. However, the underlying mechanism is still unclear. In this particular study, we found that miR-let-7d reduced collagen deposition, and this was accompanied by decreased level of iron content in myofibroblasts. Importantly, inhibition of miR-let-7d in myofibroblasts accelerated collagen deposition and promoted cell proliferation. In addition, bioinformatics prediction combined with classical dual-luciferase reporter gene assay demonstrated that the cellular iron importer divalent metal transporter 1 (DMT1) was a target gene of miR-let-7d, and the miR-let-7d mimics inhibited the expression of DMT1 in myofibroblasts. Moreover, silencing of DMT1 with small interfering RNA (siRNA) reduced the deposition of extracellular matrix. Consistent with the results in vitro, the miR-let-7d mimics effectively ameliorated hypertrophic scar fibrosis in a rabbit ear hypertrophic scar model. Taken together, our results indicated for the first time that miR-let-7d attenuated hypertrophic scar fibrosis through modulation of iron metabolism by reducing iron uptake through DMT1, which may provide a novel therapeutic strategy for hypertrophic scar.

SOD2 in platelets: with age comes responsibility

SOD2 in platelets: with age comes responsibility

Selection of RNA-Cleaving TNA Enzymes for Cellular Mg2+ Imaging.

Catalytic DNA-based fluorescent sensors have enabled cellular imaging of metal ions such as Mg2+ . However, natural DNA is prone to nuclease-mediated degradation. Here, we report the in vitro selection of threose nucleic acid enzymes (TNAzymes) with RNA endonuclease activities. One such TNAzyme, T17-22, catalyzes a site-specific RNA cleavage reaction with a kcat of 0.017 min-1 and KM of 675 nM. A fluorescent sensor based on T17-22 responds to an increasing concentration of Mg2+ with a limit of detection at 0.35 mM. This TNAzyme-based sensor also allows cellular imaging of Mg2+ . This work presents the first proof-of-concept demonstration of using a TNA catalyst in cellular metal ion imaging.

Mapping 3D grain and precipitate structure during in situ mechanical testing of open-cell metal foam using micro-computed tomography and high-energy X-ray diffraction microscopy

• 3D grain structure is mapped and tracked during loading of open-cell metal foam. • Challenges included sample size relative to beam size and large ligament motions. • X-ray CT and far-field HEDM measurements collected at incremental displacements. • New scanning and stitching methodology established for far-field HEDM measurements. • Ligament-tracking code used to express crystal orientation in local reference frame. Open-cell metal foams are ultra-low-density cellular metals with complex hierarchical structures that span bulk, cell, ligament, and sub-ligament scales and give rise to desirable properties such as high strength-to-weight ratio and excellent energy absorption. Although literature suggests that intrinsic material structures at sub-ligament length scales (e.g., grains and precipitates) play an important role in mechanical behavior of open-cell metal foams, there are very few experimental measurements of such structures in three dimensions and for meaningful volumes of foam. This study seeks to map and track the three-dimensional (3D) grain and precipitate structures of an intact volume of open-cell aluminum foam by advancing microstructural characterization techniques that leverage X-ray micro-computed tomography ( μ CT ) and far-field high-energy X-ray diffraction microscopy (FF-HEDM). A 6%-relative-density aluminum foam sample was mechanically tested in compression while μ CT and FF-HEDM measurements were collected at interrupted loading states at beamline 1-ID of the Advanced Photon Source. A new scanning strategy and reconstruction algorithm were established to enable characterization of a foam volume with diameter approximately four times wider than the nominal width of the X-ray beam. The result is a set of maps that detail both the 3D grain and precipitate structures throughout the foam volume at successive strain steps. A novel grain tracking procedure was developed to track individual grains within the foam volume by accounting for the large rigid-body motions that individual ligaments can undergo during mechanical loading. The ability to track grains and precipitate structures in three dimensions throughout large bulk deformation of ultra-low-density polycrystalline materials enables new possibilities for validating numerical models and investigating local failure mechanisms. Furthermore, the methods and procedures developed in this study could be applied to other ultra-low-density structures, such as additively manufactured lattices.

Plastic recognition and electrogenic uniport translocation of 1st-, 2nd-, and 3rd-row transition and post-transition metals by primary-active transmembrane P1B-2-type ATPase pumps.

Transmembrane P1B-type ATPase pumps catalyze the extrusion of transition metal ions across cellular lipid membranes to maintain essential cellular metal homeostasis and detoxify toxic metals. Zn(ii)-pumps of the P1B-2-type subclass, in addition to Zn2+, select diverse metals (Pb2+, Cd2+ and Hg2+) at their transmembrane binding site and feature promiscuous metal-dependent ATP hydrolysis in the presence of these metals. Yet, a comprehensive understanding of the transport of these metals, their relative translocation rates, and transport mechanism remain elusive. We developed a platform for the characterization of primary-active Zn(ii)-pumps in proteoliposomes to study metal selectivity, translocation events and transport mechanism in real-time, employing a "multi-probe" approach with fluorescent sensors responsive to diverse stimuli (metals, pH and membrane potential). Together with atomic-resolution investigation of cargo selection by X-ray absorption spectroscopy (XAS), we demonstrate that Zn(ii)-pumps are electrogenic uniporters that preserve the transport mechanism with 1st-, 2nd- and 3rd-row transition metal substrates. Promiscuous coordination plasticity, guarantees diverse, yet defined, cargo selectivity coupled to their translocation.

Preface

After successfully held for seven consecutive year, 2022 8th International Conference on Mechanical Engineering, Materials and Automation Technology (MMEAT 2022) was successfully held during May 27-29, 2022 in Qingdao, China. MMEAT 2022 is to bring together innovative academics and industrial experts in the field of materials, mechanical engineering and automation technology to a common forum. The primary goal of the conference is to promote research and developmental activities in Mechanical Engineering, Materials and Automation Technology and another goal is to promote scientific information interchange between researchers, developers, engineers, students, and practitioners working all around the world. The conference will be held every year to make it an ideal platform for people to share views and experiences in materials, mechanical engineering and automation technology and related areas.This year, more than 350 participants took part in the conference and we were greatly honored to have two professors to serve as our conference chairman and seven industry elites as our keynote speakers. The conference model was divided into two sessions, including oral presentations and keynote speeches. In the first part, some scholars, whose submissions were selected as the excellent papers, were given 15 minutes to perform their oral presentations one by one. Then in the second part, keynote speakers were each allocated 30-45 minutes to hold their speeches.In the keynote presentation part, A.Prof.Thomas Fiedler, from the University of Newcastle as our first keynote speaker. His particular interest are cellular metals for impact attenuation, damping, functional applications and lightweight design. In addition, we had Prof.Zongyu Chang, Ocean University of China. His research content mainly includes: mechanical dynamics, dynamics and control of marine equipment and human factors in offshore engineering. A.Prof.Guanglei Wu, from Dalian University of Technology. His research interests include robotic technology and their applications. And then we had Assoc.Prof. Guangming Wang, Shandong Agricultural University, China. His main research interest is hydrostatic power split continuously variable tractor transmission. Assoc.Prof. Yufei Gao, Shandong University, China. He is mainly engaged in the research of digitization and simulation, precision machining, diamond wire saw slicing and high-performance electroplated diamond wire preparation, intelligent manufacturing and intelligent equipment. Assoc.Prof.Fei Gao, Shanghai Jiao Tong University, China. His current research interests include microgrids, wireless power transfer and more electric transportation systems. Prof.Zhengfang Wang, our finale keynote speaker, from Shandong University of Technology, China.List of Committee member is available in this pdf.

Elemental Analysis for the Characterization of Antimicrobial Effects.

To address the mounting resistance challenge, novel antibiotics and unprecedented mechanisms of action are urgently needed. In this context, metals have attracted attention in two distinct ways: First, the bacterial metal ion homeostasis is essential for many cellular processes, making it a putatively lucrative antibiotic target. Metal ions are, for example, cofactors for enzymes, and they contribute to signaling and transport processes or to energy metabolism. Possible antibacterial strategies include, for example, depletion of accessible essential metals by sequestration or disruption of metal ion homeostasis by ionophores that transport ions across membranes. Second, organometallic antibiotics that contain metals as integral structural elements can provide unique chemistry with unique modes of action. Since many metal-containing structures used in synthetic chemistry are unprecedented in nature, such antibiotics could circumvent existing mechanisms of resistance. Here, we present a method for quantification of cellular metal/metalloid levels and outline the procedures necessary for antibiotic treatment of Bacillus subtilis, subsequent sample preparation, elemental analysis, and data evaluation. This approach allows to investigate disturbances of the cellular metal ion homeostasis, as well as the localization and quantitation of antibiotics that contain metals rarely found in biological systems, overall aiding in the elucidation of antibiotic mechanisms of action.

Rare earth element uptake mechanisms in plankton in the Estuary and Gulf of St. Lawrence

The global shift toward green energy alternatives escalates demands for new resources, including rare earth elements (REEs), as per their implications in various green innovations. However, our understanding of their environmental cycle, especially the interactions with aquatic organisms, remains deficient, ultimately hindering environmental protection efforts. Here, we investigate the accumulation of REEs and 18 other elements in bulk and sorted plankton collected with different net mesh sizes (30, 63, 200, 333, 500 μm) in the Estuary and Gulf of St. Lawrence in the summer and winter of 2020. We observed significant correlations between the concentrations of REEs and elements of different charge numbers and ionic radii (Ba, Co, Cs, Fe, Mn, Pb, Rb and V), indicating non-selective uptake of REEs into plankton. All these elements have their highest concentrations in the fluvial corridor and upper estuary, with more significant enrichment in phytoplankton ([La] = 26.4 ± 4.8 mg kg−1) than zooplankton ([La] = 11.6 ± 8.3 mg kg−1). Their concentrations decrease to the minimum in the Gulf of St. Lawrence, especially in zooplankton ([La] = 4.8 × 10−2 ± 3.2 × 10−2 mg kg−1). We also assessed REE patterns to identify differential REE fractionation processes and anomalies. The freshwater plankton exhibits enrichment of middle REEs (MREEs) relative to the light and heavy REEs (LREEs and HREEs), potentially because of the higher binding affinity of MREEs on cellular surface transporters and metal loading effects. In estuarine and marine settings, the REE patterns in biological samples align with suspended particles, exhibiting a linear trend with LREE enrichment. This process is more noticeable in sorted macrozooplankton, which have significant Eu anomalies (Eu/Eu* up to 2), indicating differential incorporation of REEs into the chitin shells. This study highlights the significant enrichment of REEs into freshwater primary producers and the accumulation pathway similar to other inorganic elements.

Potential effects of metal ion induced two-state allostery on the regulatory mechanism of add adenine riboswitch

Riboswitches normally regulate gene expression through structural changes in response to the specific binding of cellular metabolites or metal ions. Taking add adenine riboswitch as an example, we explore the influences of metal ions (especially for K+ and Mg2+ ions) on the structure and dynamics of riboswitch aptamer (with and without ligand) by using molecular dynamic (MD) simulations. Our results show that a two-state transition marked by the structural deformation at the connection of J12 and P1 (CJ12-P1) is not only related to the binding of cognate ligands, but also strongly coupled with the change of metal ion environments. Moreover, the deformation of the structure at CJ12-P1 can be transmitted to P1 directly connected to the expression platform in multiple ways, which will affect the structure and stability of P1 to varying degrees, and finally change the regulation state of this riboswitch.

FERONIA-like receptor 1-mediated calcium ion homeostasis is involved in the immune response

Calcium (Ca2+) is the most abundant divalent cation in plants, and cellular levels of Ca2+, which functions as a nutrient and secondary messenger, play a critical role in plant immunity. In the present study, we found that FERONIA-like receptor 1 (FLR1) positively regulates Magnaporthe oryzae resistance and that expression of FLR1 is strongly induced in response to Ca2+ deficiency. In addition, the Ca content in the shoots of flr1 was lower than that in wild-type, and the M. oryzae-sensitive phenotype of the flr1 mutant was not rescued by exogenous application of Ca2+. Moreover, RNA sequencing revealed 2,697 differentially expressed genes (DEGs) in the flr1 mutant compared with wild-type, and some of these DEGs are involved in cellular metal ion homeostasis and transition metal ion homeostasis. Changes in expression of overlapping genes between the flr1 mutant and in plants under low-Ca2+ treatment were consistent in terms of direction, indicating that FLR1 is involved in Ca2+ homeostasis. In summary, we detected FLR1-mediated resistance to M. oryzae, a phenomenon associated with Ca2+ homeostasis.

Transcriptional Responses of Stress-Related Genes in Pale Chub (Zacco platypus) Inhabiting Different Aquatic Environments: Application for Biomonitoring Aquatic Ecosystems.

Pale chub (Zacco platypus) is a dominant species in urban rivers and reservoirs, and it is used as an indicator to monitor the effects of environmental contaminants. Gene responses at the molecular level can reflect the health of fish challenged with environmental stressors. The objective of this study was to identify correlations between water quality factors and the expression of stress-related genes in Z. platypus from different lake environments (Singal and Juam Lakes). To do so, transcriptional responses of genes involving cellular homeostasis (heat-shock protein 70, HSP70; heat-shock protein 90, HSP90), metal detoxification (metallothionein, MT), and antioxidation (superoxide dismutase, SOD; catalase, CAT) were analyzed in the gill and liver tissues of Z. platypus. HSP70, HSP90, and MT genes were overall upregulated in Z. platypus from Singal Lake, which suffered from poorer water quality than Juam Lake. In addition, gene responses were significantly higher in Singal Lake outflow. Upregulation of HSP70, HSP90, and MT was significantly higher in Z. platypus gills than in the liver tissue. In addition, integrated biomarker response and heatmap analysis determined correlations between expression of biomarker genes or water quality factors and sampling sites of both lakes. These results suggest that stress-related genes used as multiple biomarkers may reflect spatial characteristics and water quality of different lake environments, and they can be used for biomonitoring and ecological risk assessment.

Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracileRS19

AbstractFunctional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaetoceros neogracile RS19, originally isolated from the Ross Sea, Antarctica, has a Zn requirement that cannot be met by Co and thus does not demonstrate a Zn/Co substitution ability as assessed by growth rate. We investigated this diatom's inability to use Co to alleviate Zn‐limited growth rates at the transporter, sensor/chaperone, and metalloenzyme level using metal quota and proteomic analyses of cultures grown over a range of Zn and Co availability. Analysis of total cellular metal quotas revealed that, although incapable of substitution, this diatom still actively assimilated dissolved Co. We furthermore observed distinct trends in the abundance levels of putative α and θ‐CAs, ZIP transporters, Zn fingers, and a Zn chaperone in response to increasing media Zn2+. Overall, Co appears to be transported into the cell, but not efficiently utilized by Zn metalloenzymes.

Divalent Metal Uptake and the Role of ZIP8 in Host Defense Against Pathogens.

Manganese (Mn) and Zinc (Zn) are essential micronutrients whose concentration and location within cells are tightly regulated at the onset of infection. Two families of Zn transporters (ZIPs and ZnTs) are largely responsible for regulation of cytosolic Zn levels and to a certain extent, Mn levels, although much less is known regarding Mn. The capacity of pathogens to persevere also depends on access to micronutrients, yet a fundamental gap in knowledge remains regarding the importance of metal exchange at the host interface, often referred to as nutritional immunity. ZIP8, one of 14 ZIPs, is a pivotal importer of both Zn and Mn, yet much remains to be known. Dietary Zn deficiency is common and commonly occurring polymorphic variants of ZIP8 that decrease cellular metal uptake (Zn and Mn), are associated with increased susceptibility to infection. Strikingly, ZIP8 is the only Zn transporter that is highly induced following bacterial exposure in key immune cells involved with host defense against leading pathogens. We postulate that mobilization of Zn and Mn into key cells orchestrates the innate immune response through regulation of fundamental defense mechanisms that include phagocytosis, signal transduction, and production of soluble host defense factors including cytokines and chemokines. New evidence also suggests that host metal uptake may have long-term consequences by influencing the adaptive immune response. Given that activation of ZIP8 expression by pathogens has been shown to influence parenchymal, myeloid, and lymphoid cells, the impact applies to all mucosal surfaces and tissue compartments that are vulnerable to infection. We also predict that perturbations in metal homeostasis, either genetic- or dietary-induced, has the potential to impact bacterial communities in the host thereby adversely impacting microbiome composition. This review will focus on Zn and Mn transport via ZIP8, and how this vital metal transporter serves as a “go to” conductor of metal uptake that bolsters host defense against pathogens. We will also leverage past studies to underscore areas for future research to better understand the Zn-, Mn- and ZIP8-dependent host response to infection to foster new micronutrient-based intervention strategies to improve our ability to prevent or treat commonly occurring infectious disease.

Design of Pb(II)-Specific E. coli-Based Biosensors by Engineering Regulatory Proteins and Host Cells.

Bacterial cell-based biosensors have been widely developed for detecting environmental toxic materials. The znt-operon in Escherichia coli is a Zn(II)-responsive genetic system and is employed in Zn(II), Cd(II), and Hg(II)-sensing biosensors. In this study, point mutations were introduced in the regulatory protein ZntR to modulate its target selectivity, and metal ion-exporting genes, such as copA and zntA, in host cells were deleted to increase cellular metal ion levels and enhance specificity. Thus, the overall responses of the E. coli cell-based biosensors toward metal(loid) ions were increased, and their selectivity, which was originally for Cd(II) and Hg(II), was shifted to Pb(II). The gene encoding ZntA, known as the Zn(II)-translocating P-type ATPase, showed an impact on the ability of E. coli to export Pb(II), whereas copA deletion showed no significant impact. Noteworthily, the newly generated biosensors employing ZntR Cys115Ile showed the capacity to detect under 5 nM Pb(II) in solution, without response to other tested metal ions within 0–100 nM. To understand the marked effect of single point mutations on ZntR, computational modeling was employed. Although it did not provide clear answers, changes in the sequences of the metal-binding loops of ZntR modulated its transcriptional strength and target selectivity. In summary, the approaches proposed in this study can be valuable to generate new target-sensing biosensors with superior selectivity and specificity, which can in turn broaden the applicability of cell-based biosensors to monitor Pb(II) in environmental systems.

Design and Validation of Additively Manufactured Metallic Cellular Scaffold Structures for Bone Tissue Engineering.

Bone-related defects that cannot heal without significant surgical intervention represent a significant challenge in the orthopedic field. The use of implants for these critical-sized bone defects is being explored to address the limitations of autograft and allograft options. Three-dimensional cellular structures, or bone scaffolds, provide mechanical support and promote bone tissue formation by acting as a template for bone growth. Stress shielding in bones is the reduction in bone density caused by the difference in stiffness between the scaffold and the surrounding bone tissue. This study aimed to reduce the stress shielding and introduce a cellular metal structure to replace defected bone by designing and producing a numerically optimized bone scaffold with an elastic modulus of 15 GPa, which matches the human’s cortical bone modulus. Cubic cell and diagonal cell designs were explored. Strut and cell dimensions were numerically optimized to achieve the desired structural modulus. The resulting scaffold designs were produced from stainless steel using laser powder bed fusion (LPBF). Finite element analysis (FEA) models were validated through compression testing of the printed scaffold designs. The structural configuration of the scaffolds was characterized with scanning electron microscopy (SEM). Cellular struts were found to have minimal internal porosity and rough surfaces. Strut dimensions of the printed scaffolds were found to have variations with the optimized computer-aided design (CAD) models. The experimental results, as expected, were slightly less than FEA results due to structural relative density variations in the scaffolds. Failure of the structures was stretch-dominated for the cubic scaffold and bending-dominated for the diagonal scaffold. The torsional and bending stiffnesses were numerically evaluated and showed higher bending and torsional moduli for the diagonal scaffold. The study successfully contributed to minimizing stress shielding in bone tissue engineering. The study also produced an innovative metal cellular structure that can replace large bone segments anywhere in the human body.

Ambient Availability of Amino Acids, Proteins, and Iron Impacts Copper Resistance of Aspergillus fumigatus

The transition metals iron and copper are required by virtually all organisms but are toxic in excess. Acquisition of both metals and resistance to copper excess have previously been shown to be important for virulence of the most common airborne human mold pathogen, Aspergillus fumigatus. Here we demonstrate that the ambient availability of amino acids and proteins increases the copper resistance of A. fumigatus wild type and particularly of the ΔcrpA mutant that lacks export-mediated copper detoxification. The highest-protecting activity was found for L-histidine followed by L-asparagine, L-aspartate, L-serine, L-threonine, and L-tyrosine. Other amino acids and proteins also displayed significant but lower protection. The protecting activity of non-proteinogenic D-histidine, L-histidine-mediated growth inhibition in the absence of high-affinity copper uptake, determination of cellular metal contents, and expression analysis of copper-regulated genes suggested that histidine inhibits low-affinity but not high-affinity copper acquisition by extracellular copper complexation. An increase in the cellular copper content was found to be accompanied by an increase in the iron content, and, in agreement, iron starvation increased copper susceptibility, which underlines the importance of cellular metal balancing. Due to the role of iron and copper in nutritional immunity, these findings are likely to play an important role in the host niche.

Physiological Adaptation of Three Wild Halophytic Suaeda Species: Salt Tolerance Strategies and Metal Accumulation Capacity.

Understanding salt tolerance mechanisms in halophytes is critical for improving the world’s agriculture under climate change scenarios. Herein, the physiological and metabolic responses of Suaeda monoica, Suaeda vermiculata, and Suaeda schimperi against abiotic stress in their natural saline environment on the east coast of the Red Sea were investigated. The tested species are exposed to different levels of salinity along with elemental disorders, including deficiency in essential nutrients (N&P in particular) and/or elevated levels of potentially toxic elements. The tested species employed common and species-specific tolerance mechanisms that are driven by the level of salinity and the genetic constitution of Suaeda species. These mechanisms include: (i) utilization of inorganic elements as cheap osmotica (Na+ in particular), (ii) lowering C/N ratio (S. monoica and S. schimperi) that benefits growth priority, (iii) efficient utilization of low soil N (S. vermiculata) that ensures survival priority, (v) biosynthesis of betacyanin (S. schimperi and S. vermiculata) and (vi) downregulation of overall metabolism (S. vermiculata) to avoid oxidative stress. Based on their cellular metal accumulation, S. monoica is an efficient phytoextractor of Cr, Co, Cu, Ni, and Zn, whereas S. vermiculata is a hyper-accumulator of Hg and Pb. S. schimperi is an effective phytoextractor of Fe, Hg, and Cr. These results highlight the significance of Suaeda species as a promising model halophyte and as phytoremediators of their hostile environments.

Old dogs, new tricks: New insights into the iron/manganese superoxide dismutase family

Superoxide dismutases (SODs) are ancient enzymes of widespread importance present in all domains of life. Many insights have been gained into these important enzymes over the 50 years since their initial description, but recent studies in the context of microbial pathogenesis have resulted in findings that challenge long established dogmas. The repertoire of SODs that bacterial pathogens encode is diverse both in number and in metal dependencies, including copper, copper and zinc, manganese, iron, and cambialistic enzymes. Other bacteria also possess nickel dependent SODs. Compartmentalization of SODs only partially explains their diversity. The need for pathogens to maintain SOD activity across distinct hostile environments encountered during infection, including those limited for essential metals, is also a driver of repertoire diversity. SOD research using pathogenic microbes has also revealed the apparent biochemical ease with which metal specificity can change within the most common family of SODs. Collectively, these studies are revealing the dynamic nature of SOD evolution, both that of individual SOD enzymes that can change their metal specificity to adapt to fluctuating cellular metal availability, and of a cell's repertoire of SOD isozymes that can be differentially expressed to adapt to fluctuating environmental metal availability in a niche.