Cell Wall Damage Research Articles

A peptide-receptor module links cell wall integrity sensing to pattern-triggered immunity.

Plants employ cell-surface receptors to perceive non- or altered-self, including the integrity of their cell wall. Here we identify a specific ligand-receptor module responsive to cell wall damage that potentiates immunity in Arabidopsis. Disruption of cell wall integrity by inhibition of cellulose biosynthesis promotes pattern-triggered immunity transcriptionally in a manner dependent on the receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2). Notably, while MIK2 can perceive peptides of the large SERINE RICH ENDOGENOUS PEPTIDE family, a single member of this family, SCOOP18, is transcriptionally induced upon cell wall damage and is required for subsequent responses such as lignification and immunity potentiation. Collectively, our results identify the SCOOP18-MIK2 ligand-receptor module as an important central hub, connecting plant cell wall integrity sensing with immunity.

Influence of cell-wall permeability on starch digestion in sweet potato cells

This study aims to evaluate the effects of cell-wall permeability on starch digestibility in sweet potato (SP) cells. Intact cells were separated from purple, red, and white SP tubers, which differ in terms of cell-wall composition and structure, and used as whole SP food models. Purple SP cells exhibited markedly higher polyphenol content but lower starch crystallinity than red and white SP cells. Thermal analysis showed that the gelatinization enthalpy of starch considerably increased due to cell wall damage and the damaged cells of purple SP increased more. The purple SP cell wall dissolved the least pectin and had the lowest permeability for amylase-sized fluorescence probes after cooking, indicating it had stronger thermal stability and resistance to amylase penetration. Among the three cell samples, purple SP cells had the highest starch digestibility (93.95%) when the cell walls were damaged; however, when cell walls remained intact, the starch digestibility (75.95%) of purple SP cells was significantly lower. Therefore, cell-wall permeability mainly controls starch digestion within SP tubers. More polyphenols may enhance cell-wall stability and barrier amylase capacity. This study provides novel insights into the function of the cell wall, which protects starchy plants from the action of amylase.

Piperazine reveals as a cell wall targeting antifungal agent against Candida albicans

Candida albicans, a dimorphic opportunistic fungal pathogen, poses a significant threat to immunocompromised patients, with infections becoming increasingly severe due to the emergence of multi-drug resistance and the lack of effective treatments. This study investigates the antifungal properties of piperazine, a heterocyclic organic compound, and its mechanism of action against Candida species. In silico molecular docking, analysis was conducted to evaluate the interaction between piperazine and cell wall synthesis proteins, complemented by pharmacokinetic ADMET studies to assess its potential as a drug candidate. The minimum inhibitory concentration for eight Candida species ranged from 256 to 512 μg/mL. Morphological changes in C. albicans treated with piperazine were observed using scanning electron microscopy, revealing significant cell wall damage. Our findings suggest that piperazine could serve as a potential cell wall-targeting antifungal agent, potentially expanding the current arsenal of antifungal therapies.

Investigating the Impact of Flavonoids on Aspergillus flavus: Insights into Cell Wall Damage and Biofilms.

Aspergillus flavus, a fungus known for producing aflatoxins, poses significant threats to agriculture and global health. Flavonoids, plant-derived compounds, inhibit A. flavus proliferation and mitigate aflatoxin production, although the precise molecular and physical mechanisms underlying these effects remain poorly understood. In this study, we investigated three flavonoids-apigenin, luteolin, and quercetin-applied to A. flavus NRRL 3357. We determined the following: (1) glycosylated luteolin led to a 10% reduction in maximum fungal growth capacity; (2) quercetin affected cell wall integrity by triggering extreme mycelial collapse, while apigenin and luteolin caused peeling of the outer layer of cell wall; (3) luteolin exhibited the highest antioxidant capacity in the environment compared to apigenin and quercetin; (4) osmotic stress assays did not reveal morphological defects; (5) flavonoids promoted cell adherence, a precursor for biofilm formation; and (6) RNA sequencing analysis revealed that flavonoids impact expression of putative cell wall and plasma membrane biosynthesis genes. Our findings suggest that the differential effects of quercetin, luteolin, and apigenin on membrane integrity and biofilm formation may be driven by their interactions with fungal cell walls. These insights may inform the development of novel antifungal additives or plant breeding strategies focusing on plant-derived compounds in crop protection.

Arabidopsis Pentatricopeptide Repeat Protein GEND2 Participates in Mitochondrial RNA Editing

Abstract In Arabidopsis, RNA editing alters more than 500 cytidines (C) to uridines (U) in mitochondrial transcripts, a process involving the family of pentatricopeptide repeat (PPR) proteins. Here, we report a previously uncharacterized mitochondrial PLS-type PPR protein, GEND2, which functions in the mitochondrial RNA editing. The T-DNA insertion in the 5′-untranslated region of GEND2, referred to as gend2-1, results in defective root development compared to wild-type (WT) plants. A comprehensive examination of mitochondrial RNA-editing sites revealed a significant reduction in the gend2-1 mutant compared to WT plants, affecting six specific mitochondrial RNA editing sites, notably within the mitochondrial genes CcmFn-1, RPSL2 and ORFX. These genes encode critical components of cytochrome protein maturation pathway, mitochondrial ribosomal subunit and twin arginine translocation subunits, respectively. Further analysis of the transcriptional profile of the gend2-1 mutant and WT revealed a striking induction of expression in a cluster of genes associated with mitochondrial dysfunction and regulated by ANAC017, a key regulator coordinating organelle functions and stress responses. Intriguingly, the gend2-1 mutation activated an ANAC017-dependent signaling aimed at countering cell wall damage induced by cellulose synthase inhibitors, as well as an ANAC017-independent pathway that retarded root growth under normal condition. Collectively, our findings identify a novel mitochondrial PLS-type PPR protein GEND2, which participates in the editing of six specific mitochondrial RNA editing sites. Furthermore, the gend2-1 mutation triggers two distinct pathways in plants: an ANAC017-dependent pathway and ANAC017-independent pathway.

In Vitro Antimicrobial Synergistic Activity and the Mechanism of the Combination of Naringenin and Amikacin Against Antibiotic-Resistant Escherichia coli.

Bacterial drug resistance is becoming an increasingly serious problem, and the development of antibacterial synergists is urgently needed. Combining existing antibiotics with promising nonantibiotic agents is one strategy that has been shown to be effective at overcoming the widespread emergence of antibiotic-resistant pathogens. In this study, we investigated the antibacterial activities and mechanism of naringenin (NG) combined with amikacin (AMK) against multidrug-resistant Escherichia coli (E. coli). We first measured the fractional inhibitory concentration (FIC) of NG combined with antibiotics via the checkerboard method. The results indicated that the combination of NG and AMK had a synergistic effect on E. coli ATCC 25922 and E. coli C7F3. In addition, this synergistic effect was verified by time-kill assays. Moreover, scanning electron microscopy (SEM) was used to observe cell morphology. The results showed that the cell wall of E. coli was destroyed. Furthermore, we assessed the leakage of alkaline phosphatase (AKP), K+, and protein. The extracellular AKP activity increased after the combinational group of 1/2MIC NG and 1/2MIC AMK, suggesting an impairment in cell wall permeability. An increase in the leakage of intracellular K+ and protein indicated an increase in cell inner membrane permeability. These results revealed that NG and AMK inhibited E. coli by damaging cell walls and membranes. In addition, PI uptake rapidly increased after treatment with NG and AMK. Confocal laser scanning microscopy (CLSM) revealed that NG caused cell wall and cell membrane damage in E. coli. In summary, our results provide a new strategy for responding to the development of E. coli drug resistance.

Exploring the antibiofilm potential of chitosan nanoparticles by functional modification with chloroquine and deoxyribonuclease

Planktonic bacteria tend to form sessile community architectures to shield resident bacteria from various environmental stresses. The formed biofilm leads to the failure of conventional antimicrobial therapy. Extracellular macromolecules, including extracellular DNA (eDNA), proteins, lipids, and polysaccharides, crosslink into gel-like structures through electrostatic forces in the mature biofilm matrix. The stereo-structural integrity and chemical inertia of the extracellular polymeric matrix result in comprehensive antimicrobial resistance to antibacterial polysaccharides. Herein, an ionic gelation method was employed to functionalize cationic chitosan nanoparticles (CSNPs) with chloroquine and deoxyribonuclease. The modification involved shifting eDNA chirality through a DNA-intercalating agent, chloroquine, and hydrolyzing an eDNA scaffold with deoxyribonuclease. The antibiofilm activity was assessed against a standard Staphylococcus aureus strain and clinical subtype isolates. Functional modifications targeting eDNA improved the chitosan anti-biofilm efficiency (residual biomass decreased from 74.2 to 90.3 % to 16.7–24.6 %) by disrupting the biofilm matrix. The functional CSNPs worked as a sensitizer prodrug, contributing to a bactericidal process of chitosan itself (cell wall damage increased from 11.38–18.16 % to 55.2–61.4 %) by dispersing the biofilm-enclosed bacteria. In vivo, the bacterial burden of infected mouse joints was reduced by 4.1 lg CFU/mL. Our results indicate the potential of this chitosan-based anti-infection strategy in biofilm-related infections.

Fraction Activities Rosella Flower (Hibiscus Sabdariffa L) as an Antibacterial and Antibiofilm Escherichia Coli ATCC 25922

Rosella flowers (Hibiscus sabdariffa L) contain alkaloid, flavonoid, saponin and tannin compounds. The compounds contained in rosella flowers have activity as antibacterial and antibiofilm. Biofilm is the ability of microorganisms that stick to the surface of objects and form a matrix. This study aims to antibacterial and biofilm activity in the form of inhibition of biofilm formation and biofilm degradation of E.coli bacteria. Rosella flowers were extracted using maceration method with 96% ethanol solvent. The thick extract was then fractionated with n-hexane, ethyl acetate and water solvents. Antibacterial activity test using diffusion and dilution methods followed by observation of cell wall and membrane damage using SEM and ion leakage with AAS. Biofilm activity test was conducted using a microplate device and readings were taken at a wavelength of 595 nm. The absorbance results of the inhibition percentage to determine the IC50 and EC50 values. The average IC50 values of biofilm formation inhibition test on extract, n-hexane fraction, ethyl acetate fraction and water fraction against E. coli bacteria are 1.059; 1.070; 0.921; 0.863. The EC50 values of biofilm degradation test on extract, n-hexane fraction, ethyl The minimum killing concentration value of rosella flower water fraction against E. coli is 25 mg/ml. the effect of the active fraction of rosella flower on E. coli bacteria is on the cell wall and cell membrane

Unravelling novel molecular targets of Thymol and Carvacrol in Candida glabrata

The emergence of non-albicans Candida as a potential causative agent of candidiasis following Candida albicans is occurring globally. It is known that phenolic cresols, thymol, and carvacrol have anti-Candida characteristics. However, there are few papers that disclose the mechanism of action, which is characterized by a damaged cell wall and ergosterol synthesis, as well as calcium-induced death in C. albicans. This work utilized in-silico docking analysis to examine the molecular targets of thymol and carvacrol in Candida glabrata. The results were then validated in vitro by antifungal susceptibility testing and growth curve analysis. The study incorporated C. glabrata deletion mutants for the target proteins to gain understanding of the function of these proteins in the antifungal effects of the two monoterpenoids. After conducting molecular docking and in silico toxicity evaluations, thymol and carvacrol were chosen for in vitro investigations. Experimental tests conducted in a laboratory setting have shown that both phytocompounds exhibit strong binding affinity towards cell wall synthesis proteins (Kre1p, Kre2p, Ecm33p), calcium channel proteins (Mid1p, Ecm7p), and proteins involved in the ergosterol synthesis pathway (Erg5p). These proteins are likely to be the specific targets for the anti-Candida properties of the two isomeric monoterpenoids in C. glabrata. Minimum inhibitory concentration (MIC50) values for thymol and carvacrol range from 50 µg/ml to 75 µg/ml. The wild-type strains exhibit a minimum fungicidal concentration of 100 µg/ml for thymol and 125 µg/ml for carvacrol. The results underscore the significance of these proteins in the fungal reaction to thymol and carvacrol and also indicate a promising opportunity for the development of novel antifungal treatment methods utilizing these proteins.

Antibacterial mechanism of the methanol extract of Thamnolia subuliformis (Ehrh.) W. Culb against Staphylococcus aureus.

Thamnolia subuliformis (Ehrh.) W. Culb is a species of lichen with edible and medicinal applications in China. Our previous studies demonstrated that the methanol extract of Thamnolia subuliformis (METS) exhibits broad antibacterial activity and stability against foodborne pathogens. This study aimed to investigate the antibacterial mechanism of METS against Staphylococcus aureus using nontargeted metabolomics, focusing on cell wall and membrane damage. The results revealed that the minimum inhibitory concentration (MIC) was 0.625mg ml-1 and that METS had good biosafety at this concentration. METS caused significant damage to the cell wall and membrane integrity, based on both morphological observation by electron microscopy and the leakage of alkaline phosphatase, protein, and nucleic acid in the cell cultures. Treatment with METS at the MIC disrupted the lipid metabolism of S. aureus, causing a decrease in the metabolism of various phospholipids and sphingolipids in the cell membrane and an increase in the ratio of saturated fatty acids to unsaturated fatty acids. Moreover, it influenced intracellular amino acid and energy metabolism. These results shed light on the antibacterial mechanism of METS against S. aureus while also serving as a reference for the further development of natural antibacterial compounds derived from Thamnolia subuliformis.

Screening of the global health priority BoxⓇ reveals potential new disinfectants against the emerging multidrug-resistant pathogen Candida auris

BackgroundCandida auris has been identified by the World Health Organization as a critical pathogen due to its invasive nature, resistance to multiple drugs, and high mortality rates in hospital outbreaks. This fungus can persist on surfaces and human skin for extended periods, complicating infection control efforts. The need for effective disinfection strategies is urgent, as current disinfectants are often ineffective against C. auris biofilms. ObjectiveThe study aimed to identify potential disinfectants from a collection of 240 compounds in the Global Health Priority Box® that are effective against C. auris, particularly strains resistant to existing options. MethodsThe research employed a screening protocol using a fluconazole-resistant strain of C. auris (149/23). Antifungal activity was assessed using the microdilution method to determine Minimum Inhibitory Concentrations (MICs) and Minimum Fungicidal Concentrations (MFCs). Additional assays were conducted to evaluate biofilm inhibition, biofilm eradication, cell membrane integrity, nucleotide leakage, sorbitol protection assay, efflux pump inhibition, and hemolysis assay. ResultsTwo compounds, Hydramethylnon (MMV1577471) and Flufenerim (MMV1794206), demonstrated significant inhibitory effects against C. auris. Hydramethylnon exhibited potent antifungal activity, inhibiting up to 93 % of fungal growth with an MFC of 16 μg/mL. Flufenerim inhibited up to 58 % of fungal growth, showing fungistatic action with an MFC greater than 4 μg/mL. Biofilm inhibition tests showed that both compounds significantly inhibited biofilm formation, with increased efficacy at higher concentrations. Both compounds showed eradication rates in both stages. Furthermore, Hydramethylnon and Flufenerim did not affect cell membrane integrity or nucleotide leakage, suggesting a mode of action not reliant on disrupting these cellular components. The sorbitol protection assay revealed that neither compound caused cell wall damage. In the efflux pump inhibition assay, Hydramethylnon did not activate efflux pumps, while Flufenerim activated efflux pumps, reducing its effectiveness. Hemocompatibility assay showed safety. ConclusionThe study highlights Hydramethylnon and Flufenerim as promising candidates for further development as disinfectants, offering potential solutions to the urgent need for effective disinfection agents against C. auris. The findings underscore the value of screening compound collections to identify novel antifungal agents and understand their mechanisms of action, thereby contributing to the advancement of new disinfection strategies in healthcare settings.

Cinnamaldehyde and baicalin reverse colistin resistance in Enterobacterales and Acinetobacter baumannii strains

PurposeColistin is used as a last resort antibiotic against infections caused by multidrug-resistant gram-negative bacteria, especially carbapenem-resistant bacteria. However, colistin-resistance in clinical isolates is becoming more prevalent. Cinnamaldehyde and baicalin, which are the major active constituents of Cinnamomum and Scutellaria, have been reported to exhibit antibacterial properties. The aim of this study was to evaluate the capacity of cinnamaldehyde and baicalin to enhance the antibiotic activity of colistin in Enterobacterales and Acinetobacter baumannii strains.MethodsThe MICs of colistin were determined with and without fixed concentrations of cinnamaldehyde and baicalin by the broth microdilution method. The FIC indices were also calculated. In addition, time-kill assays were performed with colistin alone and in combination with cinnamaldehyde and baicalin to determine the bactericidal action of the combinations. Similarly, the effects of L-arginine, L-glutamic acid and sucrose on the MICs of colistin combined with cinnamaldehyde and baicalin were studied to evaluate the possible effects of these compounds on the charge of the bacterial cell- wall.ResultsAt nontoxic concentrations, cinnamaldehyde and baicalin partially or fully reversed resistance to colistin in Enterobacterales and A. baumannii. The combinations of the two compounds with colistin had bactericidal or synergistic effects on the most resistant strains. The ability of these agents to reverse colistin resistance could be associated with bacterial cell wall damage and increased permeability.ConclusionCinnamaldehyde and baicalin are good adjuvants for the antibiotic colistin against Enterobacterales- and A. baumannii-resistant strains.

Antibacterial, Antibiofilm, and Antioxidant Activities of Aqueous Crude Gymnema inodorum Leaf Extract against Vancomycin-Resistant Enterococcus faecium.

Vancomycin-resistant Enterococcus faecium (VREF) causes nosocomial infections with high mortality and morbidity rates. This study aimed to evaluate the antibacterial and antibiofilm activities of aqueous crude Gymnema inodorum leaf extract (GIE) against the VREF ATCC 700221 strain. The antimicrobial activity of GIE against VREF was performed using disk diffusion and broth microdilution. The antibiofilm activities were evaluated using the crystal violet staining assay. The antioxidant potential was evaluated. Preliminary screening of the antimicrobial activity of 50 and 100 µg/disk of GIE against VREF revealed inhibition zones of 8.33 ± 0.58 mm and 8.67 ± 0.29 mm, respectively. Additionally, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against VREF were 125 and ≥ 250 mg/mL, respectively. SEM analysis showed that treatment with GIE caused morphological changes, including incomplete cell division, damaged cell walls, and cell content leakage, suggesting a disruption of bacterial cells. GIE also inhibited and eradicated biofilms formed by VREF. The extract exhibited antioxidant activities in the DPPH and ABTS assays. While GIE shows potential as an antibacterial and antibiofilm agent, further studies are necessary to fully understand the underlying mechanisms and optimize its use for therapeutic applications.

Decoding nature's defense dance: Mechanistic insights into biochemical and metabolic shifts in Cajanus cajan and Cajanus platycarpus during combat with the lepidopteran pest Helicoverpa armigera provide evidence for non-host plant immunity

This study provides intriguing mechanistic insights into the innate immunity of the pigeonpea wild relative, Cajanus platycarpus, in comparison to the cultivated Cajanus cajan, based on biochemical and metabolic changes during Helicoverpa armigera infestation. Over a 96 h herbivory challenge, both species exhibited 164 metabolites, with 54 undergoing significant alterations. In C. platycarpus, primary carbohydrates such as sucrose, glucose, galactose, and mannose decreased, while structural carbohydrates like xylose, talose, and tagatose accumulated, indicating cell wall damage in C. cajan. Notably, C. platycarpus down-regulated most amino acids except for phenylalanine, which is crucial for the synthesis of defense compounds. The linoleic/linolenic acid pathway, which produces jasmonic acid, was exclusively identified in C. platycarpus, highlighting active defense. As herbivory continued, levels of galactinol, mannitol, and pinitol significantly increased in C. platycarpus, while sorbitol and xylitol levels rose in C. cajan. Essentially, C. platycarpus reduced nutrient availability to insects, enhanced specific defense hormones and pathways, and maintained reactive oxygen species (ROS) equilibrium. These findings offer significant potential for understanding the biology of the wild relative and developing innovative strategies for herbivore control and food sustainability.

Biosynthesis, Antibacterial and Antifungal Activities of Ag Nanoparticles Prepared Using Piper nigrum Fruit Extract

Nanomaterials are promising alternatives to antibiotics with the potential to handle the menace of emerging multidrug resistance in pathogenic microbes. We report a simple biosynthetic approach to prepare Ag nanoparticles (NPs) with strong antibacterial and antifungal actions by using Piper nigrum fruit extract as a bioreducing agent. The biosynthesized material was characterized by SEM, AFM, TEM, HRTEM, XRD and UV–Visible absorption spectroscopy. SEM, AFM and TEM analyses provided evidence that the average diameter of the Ag NPs was 18[Formula: see text]nm, while XRD and HRTEM confirmed the FCC structure of Ag NPs. The biosynthesized Ag NPs demonstrated extremely strong antibacterial activity against Bacillus oceanisediminis and Escherichia coli, and showed extremely strong antifungal action against Schizosaccharomyces pombe, inhibiting their growth at very low concentrations of 3[Formula: see text][Formula: see text]g/mL, 4[Formula: see text][Formula: see text]g/mL and 3[Formula: see text][Formula: see text]g/mL, respectively. The molecular origin of their antibacterial and antifungal activity was studied using various molecular and biochemical assays. The results of these assays revealed a major role for reactive oxygen species (ROS) in the antifungal and antibacterial actions of the biosynthesized Ag NPs. Furthermore, morphological changes in cells and cell wall damage upon Ag NP treatment were observed by scanning electron microscopy. In addition, significant enhancement in protein leakage and fragmentation of DNA was also observed upon treatment of S. pombe, B. oceanisediminis and E. coli with Ag NPs. In conclusion, the observed extremely strong antibacterial and antifungal activities of biosynthesized Ag NPs are due to the oxidative stress triggered by ROS, causing cell wall damage and subsequent protein leakage and DNA fragmentation.

The dual-specificity kinase MoLKH1-mediated cell cycle, autophagy, and suppression of plant immunity is critical for development and pathogenicity of Magnaporthe oryzae

Cell cycle progression, autophagic cell death during appressorium development, and ROS degradation at the infection site are important for the development of rice blast disease. However, the association of cell cycle, autophagy and ROS detoxification remains largely unknown in M. oryzae. Here, we identify the dual-specificity kinase MoLKH1, which serves as an important cell cycle regulator required for appressorium formation by regulating cytokinesis and cytoskeleton in M. oryzae. MoLKH1 is transcriptionally activated by H2O2 and required for H2O2-induced autophagic cell death and suppression of ROS-activated plant defense during plant invasion of M. oryzae. In addition, the Molkh1 mutant also showed several phenotypic defects, including delayed growth, abnormal conidiation, damaged cell wall integrity, impaired glycogen and lipid transport, reduced secretion of extracellular enzymes and effectors, and attenuated virulence of M. oryzae. Nuclear localization of MoLKH1 requires the nuclear localization sequence, Lammer motif, as well as the kinase active site and ATP-binding site in this protein. Site-directed mutagenesis showed that each of them plays crucial roles in fungal growth and pathogenicity of M. oryzae. In conclusion, our results demonstrate that MoLKH1-mediated cell cycle, autophagy, and suppression of plant immunity play crucial roles in development and pathogenicity of M. oryzae.

Antibacterial Activity, Cell Wall Damage, and Cytotoxicity of Zinc Oxide Nanospheres, Nanorods, and Nanoflowers

Antibacterial Activity, Cell Wall Damage, and Cytotoxicity of Zinc Oxide Nanospheres, Nanorods, and Nanoflowers

Utilizing zinc oxide nanoparticles as an environmentally safe biosystem to mitigate mycotoxicity and suppress Fusarium graminearium colonization in wheat

The biosynthesis of zinc oxide nanoparticles (ZnONPs) offers great potential for plant disease management due to their potent antimicrobial properties and environmental safety. However, the precise mechanisms underlying their antifungal mode of action and role in suppressing mycotoxins remain unclear. This study aims to elucidate the mechanisms by which ZnONPs suppress the pathogenic fungus Fusarium graminearium, known to cause Fusarium head blight in wheat. Additionally, it investigates how ZnONPs mitigate the production of mycotoxins, which pose risks to humans and ruminants. The study demonstrates that ZnONPs, bioproduced by Pseudomonas poae (P. poae), inhibit not only fungal growth, colony formation, and spore germination, but also significantly reduce mycotoxin production of F. graminearium by inhibiting the synthesis of deoxynivalenol (DON), downregulating the FgTRI gene, and causing morphological alterations of the toxisomes. The results also highlight that ZnONPs exert significant effects on fungi through multiple mechanisms, including cell wall damage and the generation of reactive oxygen species (ROS). Moreover, ZnONPs effectively inhibit F. graminearium in wheat leaves and coleoptiles. Fluorescence microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and optical microscopy all show that ZnONPs stop F. graminearium from getting into wheat plants and colonising them. Overall, the findings of this study provide evidence that ZnONPs are highly effective in reducing F. graminearium colonization in wheat plants and effectively decreasing mycotoxin production through multiple pathways.

Role of particle size in modulating starch digestibility and textural properties in a rye bread model system

In cereal products, the use of flour containing clusters of intact cells has been indicated as a potential strategy to decrease starch digestion. Rye possesses more uniform and thicker cell walls than wheat but its protective effect against starch digestion has not been elucidated. In this study, rye flours with three different particle sizes, large (LF) (∼1700 μm), medium (MF) (∼1200 μm), and small (SF) (∼350 μm), were used to produce model bread. The textural properties of these breads were analysed using Textural Profile Analysis (TPA). The starch digestibility of both the flour and the bread was measured using Englyst’s method, while the presence of intact cell clusters was examined using Confocal Laser Scanning Microscopy (CLSM). Additionally, the disintegration of bread digesta during simulated digestion was assessed through image analysis. CLSM micrographs revealed that bread made with MF and LF retained clusters of intact cells after processing, whereas bread made with SF showed damaged cell walls. Starch digestibility in LF and MF was lower (p ≤ 0.05) than that in SF. Bread produced with MF and LF exhibited the least (p ≤ 0.05) cohesive and resilient texture, disintegrated more during digestion, and exhibited higher starch digestibility (p ≤ 0.05) than bread made with SF. These results highlight the central role of bread texture on in vitro starch digestibility.

Optimisation of garlic mustard oil macerate with respect to its antifungal activity against Candida albicans MTCC 183 and in-silico molecular docking of the volatile compounds with N-myristoyltransferase

Candida albicans infections are widespread in people and cause cutaneous and systemic infections. Optimisation of garlic mustard oil macerate (GMM) based on antifungal activity against C. albicans was done using agar diffusion method. Upon vapour diffusion assay, the volatile organic compounds of both GMM and MO were found to eradicate C. albicans. During agar diffusion, MO did not inhibit fungal growth, while undiluted GMM oil demonstrated a 26.33 ± 0.33 mm zone of inhibition. The minimum inhibitory concentration and minimum fungicidal concentration against C. albicans were 12.5%, v/v of GMM oil and 25%, v/v of GMM oil, respectively. Scanning electron microscopy analysis showed cell membrane disintegration of fungal cells by 50%, v/v of GMM oil, and MO caused no cell wall damage. In-silico analysis revealed strong binding affinity of sinigrin, ajoene, dithiin with N-myristoyltransferase. In conclusion, the optimised GMM preparation can be a potential antifungal agent against tropical C. albicans infections.