Cell Wall Constituents Research Articles

​Effect of Sowing Time on Production Potential of Maize Fodder and its Nutritive Value Before and After Ensiling

Background: This study was taken up to assess the effect of time of sowing and number of irrigations on the yield of maize cultivar J-1006 and to see their impact on the nutritive value of fresh and ensiled fodder. Methods: Maize cultivar J-1006 was sown at 50 cm row to row and 10 cm plant to plant spacing at 10 days interval in quintuplicate. The crop was harvested after 80-85 days of sowing. The J-1006 on the first date of sowing (B1) was given one irrigation and rest of the sowings (B2-B5) received two irrigations till harvest. The nutritive value (NV) of fresh and ensiled maize fodder was assessed by in vitro gas production (IVGP) technique. Result: Highest biological, DM and protein yield was recorded in B2 maize fodder while the lowest was recorded in B5. The cell wall constituents were the highest (P less than 0.01) in B1 and the lowest in B5 fodder resulting in highest (P less than 0.01) microbial biomass production (MBP) and the NV in B5 maize fodder. Irrespective of time of sowing, OM, NDF and hemicellulose contents were reduced (P less than 0.01) in ensiled maize fodder. The net gas production (NGP; P less than 0.01) and ME (P less than 0.05) were depressed and the total and individual VFAs and MBP were improved (P less than 0.01) in the ensiled maize. Irrespective of processing, the maize given two irrigations resulted in higher (P less than 0.01) MBP and NV as comparison to that given single irrigation. It was concluded that B5 maize fodder with the lowest yield given two irrigations had the best nutritive value.

Synthesis and testing for mechanical integrity of nano cellulose composites

This scientific research focused on extraction of the polymer from an appropriate source and preparation of films with mechanical integrity and desirable chemical properties. Reinforcement of said films with nano-fillers like Nano silica and clay were administered to reinforce their mechanical properties. Further development on these films would contribute to eliminating plastics and replacing them with better and sustainable materials. Cellulose may be a biopolymer found in plant cell walls which will be derived from biomass sources like sugarcane. It is one among the main constituents of plant cell walls alongside hemicellulose and lignin. “Cellulose consists of long unbranched fibers of glucose held together by hydrogen bonds”. It is widely utilized in paper, plastics, coatings and casings.

A GH115 α-glucuronidase structure reveals dimerization-mediated substrate binding and a proton wire potentially important for catalysis.

Xylan is a major constituent of plant cell walls and is a potential source of biomaterials, and the derived oligosaccharides have been shown to have prebiotic effects. Xylans can be highly substituted with different sugar moieties, which pose steric hindrance to the xylanases that catalyse the hydrolysis of the xylan backbone. One such substituent is α-D-glucuronic acid, which is linked to the O2' position of the β-1,4 D-xylopyranoses composing the main chain of xylans. The xylan-specific α-glucuronidases from glycoside hydrolase family 115 (GH115) specifically catalyse the removal of α-D-glucuronic acid (GlcA) or methylated GlcA (MeGlcA). Here, the molecular basis by which the bacterial GH115 member wtsAgu115A interacts with the main chain of xylan and the indirect involvement of divalent ions in the formation of the Michaelis-Menten complex are described. A crystal structure at 2.65 Å resolution of wtsAgu115A originating from a metagenome from an anaerobic digester fed with wastewater treatment sludge was determined in complex with xylohexaose, and Asp303 was identified as the likely general acid. The residue acting as the general base could not be identified. However, a proton wire connecting the active site to the metal site was observed and hence a previous hypothesis suggesting a Grotthuss-like mechanism cannot be rejected. Only a single molecule was found in the asymmetric unit. However, wtsAgu115A forms a dimer with a symmetry-related molecule in the crystal lattice. The xylohexaose moieties of the xylohexaose are recognized by residues from both protomers, thus creating a xylohexaose recognition site at the dimer interface. The dimer was confirmed by analytical size-exclusion chromatography in solution. Kinetic analysis with aldouronic acids resulted in a Hill coefficient of greater than 2, suggesting cooperativity between the two binding sites. Three Ca2+ ions were identified in the wtsAgu115A structures. One Ca2+ ion is of particular interest as it is coordinated by the residues of the loops that also interact with the substrate. Activity studies showed that the presence of Mg2+ or Mn2+ resulted in a higher activity towards aldouronic acids, while the less restrictive coordination geometry of Ca2+ resulted in a decrease in activity.

Role for a Lytic Polysaccharide Monooxygenase in Cell Wall Remodeling in Streptomyces coelicolor.

ABSTRACTPeptidoglycan is a major constituent of the bacterial cell wall and an important determinant for providing protection to cells. In addition to peptidoglycan, many bacteria synthesize other glycans that become part of the cell wall. Streptomycetes grow apically, where they synthesize a glycan that is exposed at the outer surface, but how it gets there is unknown. Here, we show that deposition of the apical glycan at the cell surface of Streptomyces coelicolor depends on two key enzymes, the glucanase CslZ and the lytic polysaccharide monooxygenase LpmP. Activity of these enzymes allows localized remodeling and degradation of the peptidoglycan, and we propose that this facilitates passage of the glycan. The absence of both enzymes not only prevents morphological development but also sensitizes strains to lysozyme. Given that lytic polysaccharide monooxygenases are commonly found in microbes, this newly identified biological role in cell wall remodeling may be widespread.

Advantages of MW-assisted water extraction, combined with steam explosion, of black alder bark in terms of isolating valuable compounds and energy efficiency

“Green” extraction of black alder bark using deionized water as an alternative to organic solvents and microwave (MW) - induced heating instead of convective/conductive heating was studied. An extractor of original construction with the MW capacity of 2.4 kW, equipped with a pressurized extraction chamber (V = 1350 cm3) and a condenser section, was used. The MW-assisted heating of the suspension up to the desirable temperature was performed with a uniform high rate of ~ 30 ℃ min−1. The yield of the black alder bark water extracts obtained by MW-assisted extraction varied from 15.4% to 26.4% (on bark dry matter) increasing with increasing extraction temperature (70–150 ℃) and increasing duration of the isothermal step of extraction (0–30 min). The electricity consumption per dry weight of obtained products needed for MW-assisted heating of the substrate containing suspension varied from 6.2 to 32.0 kWh kg−1 being higher at higher extraction temperature and longer duration of isothermal heating step. UHPLC-ESI-MS/MS, GC-FID, Py-GS/MS/FID, UV spectroscopy methods revealed that the main constituents of the obtained black alder bark water extracts were diarylheptanoids, condensed tannins, carbohydrates, organic acids, flavonoids, lignin related phenols. The MW-induced depolymerization of alder bark cell wall constituents (hemicellulose, lignin) evidenced by Py-GC/MS/FID, GC-FID, wet chemistry analyses of extracts and bark residues after extraction, resulted in the disruption of the bark cell wall and isolation of metabolites bound within it by microwaves-assisted extraction (MAE). Therefore, the black alder bark water extracts, obtained by MAE at 70–110 ℃, compared to those isolated by advanced accelerated solvent extraction (ASE) at a given temperature, have higher diversity and higher contents of different groups of phenolic extractives, including a higher content of their major class in alders – diarylheptanoids. The increase of the extraction temperature above 110 ℃ and the duration of extraction up to 30 min led to an increase of the content of carbohydrates and noticeable decrease of phenolics content in extracts, including the dominating diarylheptanoid - oregonin. The content of oregonin, having a high antioxidant activity and valuable biological activities, was in focus of our research and practical interest. MAE with dynamic heating to 90 ℃ without keeping of isothermal heating is the most attractive for obtaining of oregonin-enriched extracts, containing up to 57% of oregonin, in contrast to ASE water extracts with the maximum oregonin content of 39%. In this regime, two-fold less energy consumption (7 vs 15 kWh kg−1) and two and a half times more productivity (1.5 vs 0.6)% min−1 was achieved in comparison to the case of the ASE method used as reference.

Internalization of Polymeric Bacterial Peptidoglycan Occurs through Either Actin or Dynamin Dependent Pathways.

Peptidoglycan (PGN), a polymeric glycan macromolecule, is a major constituent of the bacterial cell wall and a conserved pathogen-associated molecular pattern (PAMP) that triggers immune responses through cytosolic sensors. Immune cells encounter both PGN polymers and hydrolyzed muropeptides during infections, and primary human innate immune cells respond better to polymeric PGN than the minimal bioactive subunit muramyl dipeptide (MDP). While MDP is internalized through macropinocytosis and/or clathrin-mediated endocytosis, the internalization of particulate polymeric PGN is unresolved. We show here that PGN macromolecules isolated from Bacillus anthracis display a broad range of sizes, making them amenable for multiple internalization pathways. Pharmacologic inhibition indicates that PGN primarily, but not exclusively, is internalized by actin-dependent endocytosis. An alternate clathrin-independent but dynamin dependent pathway supports 20–30% of PGN uptake. In primary monocytes, this alternate pathway does not require activities of RhoA, Cdc42 or Arf6 small GTPases. Selective inhibition of PGN uptake shows that phagolysosomal trafficking, processing and downstream immune responses are drastically affected by actin depolymerization, while dynamin inhibition has a smaller effect. Overall, we show that polymeric PGN internalization occurs through two endocytic pathways with distinct potentials to trigger immune responses.

Insights into the molecular basis of hypergravity-induced root growth phenotype in bread wheat (Triticum aestivum L.).

Hypergravity is a condition where the force of gravity exceeds that on the surface of the Earth and can be simulated by centrifugation. Previously, a significant increase in root growth phenotype was observed when wheat seeds were exposed to hypergravity (10g for 12h). In the present study, we investigated the molecular basis of this change through root transcriptome. The data revealed a total of 3765 up-regulated and 2102 down-regulated transcripts in response to hypergravity. GO enrichment analysis revealed hormonal responses, cell division, and cell-wall-related terms were significantly enriched in hypergravity. The increased isoform level expression of transcripts involved in auxin biosynthesis, transport, and signaling was observed. Further, enhanced expression of cell division transcripts and down-regulation of cell number regulator genes suggests rapid cell division. Overexpression of cellulose and hemicellulose biosynthesis transcripts suggests demand for cell-wall constituents. Collectively, this study identified candidate genes associated with hypergravity-induced enhanced root growth.

Functional Classification and Characterization of the Fungal Glycoside Hydrolase 28 Protein Family.

Pectin is a major constituent of the plant cell wall, comprising compounds with important industrial applications such as homogalacturonan, rhamnogalacturonan and xylogalacturonan. A large array of enzymes is involved in the degradation of this amorphous substrate. The Glycoside Hydrolase 28 (GH28) family includes polygalacturonases (PG), rhamnogalacturonases (RG) and xylogalacturonases (XG) that share a structure of three to four pleated β-sheets that form a rod with the catalytic site amidst a long, narrow groove. Although these enzymes have been studied for many years, there has been no systematic analysis. We have collected a comprehensive set of GH28 encoding sequences to study their evolution in fungi, directed at obtaining a functional classification, as well as at the identification of substrate specificity as functional constraint. Computational tools such as Alphafold, Consurf and MEME were used to identify the subfamilies’ characteristics. A hierarchic classification defines the major classes of endoPG, endoRG and endoXG as well as three exoPG classes. Ascomycete endoPGs are further classified in two subclasses whereas we identify four exoRG subclasses. Diversification towards exomode is explained by loops that appear inserted in a number of turns. Substrate-driven diversification can be identified by various specificity determining positions that appear to surround the binding groove.

Simultaneous elevation of acid-insoluble lignin and syringyl lignin is the preliminary Cd detoxification strategy in a Cd pollution-safe cultivar (Cd-PSC) of Brassica parachinensis L.

Cadmium (Cd) immobilization in the cell wall is responsible for Cd detoxification in Cd-accumulating cultivars. Lignin is the dominant constituent of the secondary cell wall (SCW), but its role in Cd compartmentalization is poorly understood. The mechanism of Cd compartmentalization by lignin was thus investigated herein. Lignin content and composition, as well as related genes and proteins, were analyzed in the Cd pollution-safe cultivar Brassica parachinensis L. ‘SJ19’. The lignin microstructural regions and Cd affinities were characterized by 2D-heteronuclear single quantum coherence nuclear magnetic resonance (HSQC NMR) and density functional theory (DFT). Lignin combined with 14.2% of the total Cd in SJ19, with a higher Cd distribution in acid-insoluble lignin (AIL). Additionally, the proportion of AIL increased under Cd treatment. The increase in the proportion of the lignin aromatic region syringyl (S) and decrease in the guaiacyl (G) proportion were in accordance with the significant up-regulation of peroxidase P7 and down-regulation of caffeoyl-CoA O-methyltransferase At1g67980 under Cd stress. Moreover, the Cd affinity associating with the lignin aromatic region ranked as S > G> H (p-hydroxyphenyl). Therefore, rather than lignin biosynthesis, the simultaneous elevation of AIL and S lignin was recognized as the preliminary strategy for lignin compartmentalization of Cd in SCW for the first time herein. This is beneficial for Cd detoxification in the Cd pollution-safe cultivar of B. parachinensis.

Luminescent β-diketonate coordinated europium(III) sensor for rapid and sensitive detection of Bacillus Anthracis biomarker

The hazardous B. Anthracis are harmful pathogens causes anthrax could be potentially used as biothreat agents or bioweapons and have severe consequences to public health. These bacterial endospores contain dipicolinic acid (DPA) as calcium dipicolinate (CaDPA) as a primary cell wall constituent (1 M, 1015 molecules/spore). The anthrax attacks in USA by shipping spores in postal mails have triggered the hunt for a simple, rapid, sensitive, and selective detection of Bacillus Anthracis spores. Therefore, a fast and sensitive sensing platform for anthrax spores is critical to contain such biothreats and outbreaks of infection. Herein, we intend to develop a sensitive and selective optical detection strategy for anthrax spore biomarker based on DPA-sensitized lanthanide luminescence. The anionic hard DPA2− strongly binds with hard Ln3+ forming thermodynamically stable complex and sensitizing unique LnIII time-resolved luminescence originated from f-f transitions with a high S/N ratio. Here, we employed and repurposed a simple luminescent EuIII complex: [Eu(TTA)3(H2O)2] (1) (TTA: 3-thenoyltrifluoroacetonate) for the detection of the DPA2− biomarker. Complex 1, when titrated with DPA, displayed instant Switch-ON optical response by 10-fold enhancement in the time-resolved luminescence (TRL) intensity of the 5D0→7F2 transition, which is sensitive to the changes in the symmetry of the EuIII primary coordination sphere. Upon binding with DPA, we also observed shifting of the excitation wavelength (λex) from ∼345 nm to 275 nm in the presence of ∼3.0 mol equivalent of dipicolinic acid. After stoichiometric reaction with DPA, the resulting complex has a long-lived, highly stable excited state of the EuIII ion with a lifetime of 2100 μs in solution compared to only 260 μs for the original probe. We proposed possible displacement of the TTA− and H2O from the primary coordination sphere of the EuIII ion by potent chelating antenna ligand DPA2−. This reaction results in the formation of nine-coordinated [Eu(DPA)3]3- complex in solution confirmed by ESI-MS analysis. The luminescence response of the complex 1 is found highly selective for DPA in the presence of various interfering carboxylic acids and quantitatively able to detect up to 1.20 μM.

Pectin self-assembly and its disruption by water: insights into plant cell wall mechanics.

Plant cell walls undergo multiple cycles of dehydration and rehydration during their life. Calcium crosslinked low methoxy pectin is a major constituent of plant cell walls. Understanding the dehydration-rehydration behavior of pectin gels may shed light on the water transport and mechanics of plant cells. In this work, we report the contributions of the microstructure to the mechanics of pectin-Ca gels subjected to different extents of dehydration and subsequent rehydration. This is investigated using a pectin gel composition that forms 'egg-box bundles', a characteristic feature of the microstructure of low methoxy pectin-Ca gels. Large amplitude oscillatory shear (LAOS) rheology along with small angle neutron scattering and near infrared (NIR) spectroscopy on pectin gels is used to elucidate the mechanical and microstructural changes during dehydration-rehydration cycles. As the extent of dehydration increases, the reswelling ability, strain-stiffening behavior and yield strain decrease. These effects are more prominent at faster rates of dehydration and are not completely reversible upon rehydration to the initial undried state. Microstructural changes due to the aggregation of egg-box bundles and single chains and the associated changes in the water configurations lead to these irreversible changes.

Influence of bioprocessing treatments on phytochemical and functional properties, in vitro digestibility, protein secondary structure and morphological characteristics of Indian barnyard millet flour

SummaryThe objective of the present investigation was to assess the effects of different bioprocessing treatments on Indian barnyard millet (Echinochloa frumentacea) at preliminary optimised conditions including soaking (12 h at 25 °C), germination (48 h at 25 °C), lactic acid fermentation (20 h at 38 °C) and combination of aforesaid treatments. Anti‐nutritional factors were significantly (P < 0.05) lowered (5–70% reduction) during different treatments. Enhanced enzymatic activity further resulted in liberation of bound phytochemical compounds (phenolic acid and flavonoids) in addition to their synthesis, which enhanced the antioxidant potential by several folds. Variation in the hydrophobic‐hydrophilic characteristics of barnyard millet flour during given treatments modified the techno‐functionality in terms of hydration properties, surface characteristics, gelation and tristimulus colour parameters. In vitro starch and protein digestibility were significantly (P < 0.05) improved from 6.49 to 23.65 mg maltose released per 100 g and 72.21 to 80.48 g hydrolysed protein per 100 g, respectively. Morphological changes as revealed by scanning electron microscopy showed that bioprocessing of barnyard millet flour disrupted the starch‐protein matrix, cell wall constituents and starch‐fibre association which made the protein and starch molecules susceptible to in vitro digestibility conditions. Further, Pearson’s correlation matrix was employed to determine the relationship among different observations.

Absolute abundance values reveal microbial shifts and co-occurrence patterns during gut microbiota fermentation of dietary fibres in vitro

The compositional alterations of human colonic microbiota when fermenting dietary fibres (DF) have been well studied. However, there is a challenge in quantifying microbiota responses to DF in absolute terms, and thus the extents to which these microbes grow on diverse DF are unclear. This study aimed to compare relative and absolute abundance datasets, to investigate how human faecal microbiota altered during the in vitro fermentation of arabinoxylan, mixed-linkage beta-glucan and bacterial cellulose (representing major wheat cell wall constituents), and a physical mixture of the three. Different microbial shifts occurred per DF, where the absolute abundance of total bacteria was observed to peak at 12 h, while this trend was not observed for the relative abundance data. The DF mixtures more efficiently supported microbial growth than the individual components. Additionally, the absolute abundance of the genera Faecalibacterium, Roseburia, Anaerostipes and the Eubacterium hallii group positively correlated to butyrate production, but no significant (P < 0.05) correlations were found based on the relative abundance data. While 95 genera were common to the DF types, interestingly, their co-occurrence patterns in absolute terms vastly differed, with 45.6% specific edges unique to the BC co-occurrence network. Therefore, applying absolute quantification is recommended for experiments focusing on how microbiota respond to DF.

In-Silico Validation of Prosopis ciniraria Therapeutic Peptides Against Fungal Cell Wall: Better Treatment Strategy for Fungal Diseases.

Prosopis cineraria commonly known as Druce are valuable herb that holds antibacterial role, antifungal properties. We identified different peptides from this plant by deploying CADD (Computer-aided-drug-designing) approaches, these peptide sequences are as follows seq1 (RHDEEEEKAKV),seq3(KSNSTVEISQNVQSVDSSKM),seq4(KQVAEMNKPAVGSKTSDANHDLKS),seq5(KTKSAGNDSIQSTKPVPSALTVDKA),seq6(RELEDSNIHHVAASVVLESKSSRT), and seq8(LYSKVELHPFGLHNLGNSCYANAVFSV), these peptides holds therapeutic properties as shows interaction with chitin, a major constituent of fungal cell wall. Molecular docking was conducted by using AutoDock-Vina tool and the results were found to be promising where all binding energies were found in the range of − 9.1 to − 7.5 kcal/mol, it indicates strong binding of peptide sequences with chitin molecule. Even the toxicity analysis supports the considered peptide sequences to hold therapeutic role against fungus with non-toxic effect on humans. These peptides were successfully predicted as important therapeutic agents of P. cinerariaseed that can initiate chitin breakdown, due to their possible strong interaction with fungal cell wall and it also suggests this medicinal plant holds the key for multiple fungal disease treatments. This study will open new research dimensions and integration of computational biology with microbial pathology that will assist scientific and medical community to develop rapid disease prevention strategies against fungal pathogenesis.

Differential Response of Two Tomato Genotypes, Wild Type cv. Ailsa Craig and Its ABA-Deficient Mutant flacca to Short-Termed Drought Cycles.

Two tomato genotypes with constitutively different ABA level, flacca mutant and wild type of Ailsa Craig cv. (WT), were subjected to three repeated drought cycles, with the aim to reveal the role of the abscisic acid (ABA) threshold in developing drought tolerance. Differential responses to drought of two genotypes were obtained: more pronounced stomatal closure, ABA biosynthesis and proline accumulation in WT compared to the mutant were compensated by dry weight accumulation accompanied by transient redox disbalance in flacca. Fourier-transform infrared (FTIR) spectra analysis of isolated cell wall material and morphological parameter measurements on tomato leaves indicated changes in dry weight accumulation and carbon re-allocation to cell wall constituents in flacca, but not in WT. A higher proportion of cellulose, pectin and lignin in isolated cell walls from flacca leaves further increased with repeated drought cycles. Different ABA-dependent stomatal closure between drought cycles implies that acquisition of stomatal sensitivity may be a part of stress memory mechanism developed under given conditions. The regulatory role of ABA in the cell wall restructuring and growth regulation under low leaf potential was discussed with emphasis on the beneficial effects of drought priming in developing differential defense strategies against drought.

Potential of waste flowers used as feed additive on the performance of goat kids

The present study was conducted to assess the impact of temple waste flowers as feed additive on the growth of goat kids. Marigold (Calendula officinalis) flowers constituted the bulk of WFs with negligible red roses (Rosa indica L). Marigold flowers as compared to rose flowers contained high (P&lt;0.01) total phenols and flavonoids; and low (P&lt;0.01) anthocyanins, saponins and vitamin C content. The in vitro studies revealed that the supplementation of WFs at 3 and 5% improved (P&lt;0.01) digestibility of nutrients and VFAs production as compared to control. NGP at t½ increased (P&lt;0.05) linearly up to 4% WFs supplementation and methane production decreased (P&lt;0.05) at all levels of supplementation. Fifteen Beetal goat kids divided into three equal groups were fed TMR, or TMR supplemented with WFs at 3 or 5% of DM intake for 90 days. The daily DM consumed by the animals was similar in all the groups. The digestibility of proximate constituents at 3% level of WF was comparable with control TMR, but depressed (P&lt;0.05) at 5% level of supplementation. The digestibility of cell wall constituents and N retention were not affected by WF supplementation. The average daily gain in weight was improved considerably. It was concluded that bio-active compounds present in WFs reduced the methane emission, resulting in improvement in nutrient utilization and growth of goat kids.

Using In vitro Technique to evaluation adding Nano cobalt to some roughages

This paper is evaluating the addition of nanocobalt on dry matter, organic matter, cel-lulose and hemicellulose degradability of In-vitro gases technique. Nanocobalt as an addi-tive were used as 0, 25, 50, 75, 100 and 125% from the animal requirements on DM basis. The regular ration (1:1 concentrate: clover hay) was incubated for 24 hours. Then 24 and 48 hours of dry matter, organic matter, cellu-lose and hemicellulose were used to research the impact of adding 25 and 50% nanocobalt to some roughages (rice, bean, wheat, bagasse and peanut) straws using In-vitro disappear-ance. Results of In-vitro gas indicated that the DMD degradability values were increased (P<0.05) in 50 and 75 %, while the highest of OMD was in 25 and 50 % of nanocobalt. The nanocobalt had no effect on pH, but had a greater influence on the values of ammonia and TVF’s, as well as the degradability of cell wall components with microbial proteins. In control ration, the gas production was high, adding cobalt (100 %) and nanocobalt (25 and 50 %) than other of addition. High values of microbial protein (MP) and microbial protein efficiency (EMP) recorded for all different nanocobalt added. Nanocobalt 25% output gas of DM, NDF, ADF, cellulose and hemicellu-lose showed increase (P<0.05) compared to any additional levels. It concluded that the ad-dition of nanocobalt increased the production of gas, ammonia, TVF’s, metabolizable energy and degradability of cell wall constituents. It showed that the addition of nanocobalt had a stronger effect on disappearance of all roughages as DM, OM, NDF, ADF, cellulose and hemicellulose. Furthermore, the addition of 25% nanocobalt had a significant effect (P<0.05) on improving In-vitro DM and OM disappearance of all roughages except bagasse had a significant effect in add 50 % nanoco-balt.

AN EXHAUSTIVE REVIEW ON EMERGING DRUG TARGETS OF TUBERCULOSIS WITH SPECIAL EMPHASIS ON CELL WALL SYNTHESIS

Tuberculosis (TB) is one of the top 10 causes of mortality and morbidity. Worldwide, yet, it has been over 60 years since a novel drug was introduced in market to treat the disease exclusively. Increased number of drug resistant TB cases has prompted the search for novel potent anti-TB drug. Mycobacterial cell wall has unique structure which provides integrity to the cell. The future development of new potent anti-TB drug targets is associated with the synthesis of various cell wall constituents; the structural and genetic information about mycobacterial cell wall envelope is now available. In the present review, we have focused on prospective drug targets that can be optimum triumph for successful drug candidate.

Mushroom-derived polysaccharides as antitumor and anticancer agent: A concise review

Nowadays, mushrooms with enhanced medicinal properties are being focused on finding such compounds that could modulate the immune systems of the human body. Mushrooms are extensively known for their antimicrobial, antidiabetic, antiviral, hepatoprotective, antitumor, and immunomodulatory properties owing to the presence of various bioactive components. However, a few of them are characterized and reported so far. Various polysaccharides, including β-glucans, are the principal constituent of the mushroom cell wall and play a significant role in their biological activity. This review aimed to focus on a concise report on the extraction process of the active ingredients from a mushroom with some therapeutic applications. Here, we have briefly described the medicinal properties of some commonly used mushroom extracts or their derivatives. It is interesting to note that mushroom is a potential source of many bioactive products that boost immunity. Thus, the development of functional medicinal food is essential for human welfare.

The placenta of Physcomitrium patens: transfer cell wall polymers compared across the three bryophyte groups.

Following similar studies of cell wall constituents in the placenta of Phaeoceros and Marchantia, we conducted immunogold labeling TEM studies of Physcomitrium patens to determine the composition of cell wall polymers in transfer cells on both sides of the placenta. 16 monoclonal antibodies were used to localize cell wall epitopes in the basal walls and wall ingrowths in this moss. In general, placental transfer cell walls of P. patens contain fewer pectins and far fewer AGPs than those of the hornwort and liverwort. P. patens also lacks the differential labeling that is pronounced between generations in the other bryophytes. In contrast, transfer cell walls on either side of the placenta of P. patens are relatively similar in composition with slight variation in HG pectins. Compositional similarities between wall ingrowths and primary cell walls in P. patens suggest that wall ingrowths may simply be extensions of the primary cell wall. Considerable variability in occurrence, abundance, and types of polymers among the three bryophytes and between the two generations suggests that similarity in function and morphology of cell walls does not require a common cell wall composition. We propose that the specific developmental and life history traits of these plants may provide even more important clues in understanding the basis for these differences. This study significantly builds on our knowledge of cell wall composition in bryophytes in general and transfer cells across plants.