The stability and compactness of myelin structure and brain homeostasis depend on MBP and glial cell plasma membrane interactions. In order to get more detailed mechanisms of this interaction, the MBP of different concentrations interacting with plasma membrane (POPC/POPE/POPS/Cholesterol (Chol)) model to form bionic membrane was studied by atomic force microscopy (AFM) and Langmuir monolayer technology. The surface pressure(π)-area(A) curve is analyzed by the elastic modulus ([Formula: see text]) and two-dimensional virial equation of state (2D-VES), and the second virial coefficient of the interaction between MBP and plasma membrane molecules was calculated. (i) According to two-dimensional virial equation, it could be analyzed that with the increase of MBP concentration in the subphase, the value of the second virial coefficient increases also, which indicates that MBP is absorbed into lipid membrane of the plasma membrane model at low surface pressure and that the interaction between the molecules is spatial repulsive force, and (ii) in the monolayers with MBP, resulting in an increasing mean molecular area and monolayer stability due to hydrophobic and electrostatic interactions between the positively charged MBP with hydrophobic residues and negatively charged POPS and neutral lipid (POPC, POPE). AFM surface topographic results correspond to the results of the curve analysis, indicating that MBP of different concentrations has significant influences on alignment and conformation of plasma membrane, which is of great medical value and biological significance to the application of interaction between MBP and myelin lipid membrane in treatment of central nervous diseases. Adsorption model of interaction between MBP and plasma membrane model.