Cell Membranes Of Patients Research Articles

Prognostic Value of CD14 Expression in Peripheral Blood Mononuclear Cell Membrane in Patients with Severe COPD Complicated with Pulmonary Infection.

Severe chronic obstructive pulmonary disease (COPD) patients with pulmonary infections face higher morbidity and mortality. To investigate mononuclear cell membrane CD14 as a prognostic marker for their outcome. A total of 311 participants were included: 122 in the coinfection group, 127 in the severe COPD group, and 62 in the control group. The patients in the coinfection group were categorized into survival (n=106) and death (n=16) groups based on hospitalization prognosis. The CD14%, CD14MFI, and CD14IND values were compared between the groups. Death risk factors were assessed by COPD grading, FEV1% pred, FEV1/FVC, CD14%, CD14MFI, and CD14IND. Correlations between CD14 parameters and mortality, COPD grade, FEV1%pred, and FEV1/FVC were analyzed. The critical value for CD14IND to predict patient death was determined and survival rates were compared between the high and the low-risk groups. CD14% values were significantly lower in the COPD and co-infection groups than in the control groups (p<0.05). The survival group showed a steady increase in mCD14 expression, while the death group showed fluctuating low levels. Low value of CD14% was identified as a risk factor for death and correlated with mortality and COPD severity (p<0.001). CD14IND≤74.36 predicted death with 91.22% sensitivity and 95.51% specificity. The high-risk group had a significantly lower 30-day survival rate (68.42%) compared with the low-risk group (95.24%) (log-rank χ2=10.067, p=0.002). The CD14 parameters of mononuclear cell membranes prove to be promising markers for predicting prognosis and death in severe COPD patients with lung infection.

Changes in the spectrum of protein components and the concentration of phospholipids of the erythrocyte membrane in patients with ulcerative colitis

Ulcerative colitis is a chronic inflammatory bowel disease with unclear etiology. An insufficient amount of data on the etiopathogenetic mechanisms of the disease creates opportunities to research them at the cellular level, including the membrane level. Since the functional state of the cytoplasmic membrane largely depends on protein-lipid interactions, we studied the effect of the proportional ratio of three proteins (glucose transporter, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glutathione-S-transferase (GST)) on the level of phosphatidylethanolamine and phosphatidylcholine in the erythrocyte membrane of patients with ulcerative colitis during an acute attack. The study involved 51 patients with ulcerative colitis during the acute attack and 26 clinically healthy individuals. The groups are comparable by gender and age to study the proteins of the cytoplasmic membrane, Laemmli electrophoresis technique in polyacrylamide gel was used, followed by an assessment of the intensity of protein bands staining on electropherograms. Phospholipid fractions were obtained using thin-layer chromatography and their content was assessed using the Mathcad 2001 Professional system. Statistical processing was carried out by the program Statistica 10.0 for Windows. Changes of phosphatidylcholine and phosphatidylethanolcholine levels in patients and healthy individuals are not of the same type and are interrelated with changes in the proportion of membrane proteins of the glucose transporter, GAPDH and GST. The proportions of proteins that affect the level of phospholipids have calculated. In patients with ulcerative colitis, a decrease in the level of the studied membrane lipids have revealed in comparison with the clinically healthy group, in addition, an increase in the proportion of the glucose transporter and a decrease in the proportion of GST have noted. In addition, the relationship between an increase in the level of the cytoplasmic membrane phospholipids in ulcerative colitis and a change in the proportion of GAFDH in a smaller direction, which slows down the process of glucose oxidation, was determined. The obtained data confirm the strengthening of the processes of antioxidant protection and the increased need for energy supply of the cell membrane in patients with ulcerative colitis during an acute attack.

Indicators of adrenergic reactivity of erythrocyte membranes in patients with myocardial infarction and nonobstructive atherosclerosis of the coronary arteries after 1 year

Abstract Funding Acknowledgements Type of funding sources: None. Background We hypothesized that MINOCA patients have distinctive features of sympatho-adrenal system (SAS) activation in comparison with patients with stenosis atherosclerosis which can play a significant role in the development of ischemic events at the time of the index hospitalization and after 1 year. Aim To study the parameters of β-adrenoreception of cell membranes in patients with MINOCA compared with patients with AMI and single-vessel coronary artery disease after 1 year. Material and methods: The study is non-randomized open controlled. Adrenergic reactivity of the body was assessed by the method for assessing the β-adrenergic reactivity of erythrocyte membranes (β-ARM) for studying the parameters of adrenergic reception of cell membranes. This parameter (β-ARM) was studied upon admission, at days 2, 4 and 7 and 1 year after AMI. The normal level of β- ARM &amp;lt;20 rel.units. Results The study included 40 patients with AMI (19 patients in the main group and 21 patients in the control group). Three patients (15.7%) with diagnosed acute myocarditis were excluded from the analysis. The median age in the main and control groups was 66 (54; 70) years and 59 (55; 65) years, respectively. These groups were different at the admission in such parameters: in smoking frequency (31,3% vs 52.3%), history of angina pectoris (62,5% vs 28,5%), time of admission to the hospital (390 min. vs 180 min.) and thrombolytic therapy at the prehospital phase (3% vs 11%), p &amp;lt; 0,05. The median β-ARM in MINOCA patients upon admission was 41.7 (29.0; 61.5) rel. units, 1 day - 48.6 (38.5; 57.3) rel. units, 4 days - 49, 4 (39.0; 63.3) rel. units, 7 days - 53.5 (35.2; 67.7) rel. units, after 1 year - 35.7 (25.5; 42.6) rel. units. In the control group, the median β-ARM upon admission was 52.5 (25.4; 64.5) rel. units, 1 day –51.6 (28.3; 56.9) rel. units, 4 days - 48, 5 (34.9; 61.2) rel. units, 7 days - 45.1 (32.2; 68.9) rel. units, after 1 year - 20.8 (14.8; 29.3). In MINOCA patients β-ARM indices in the early postinfarction period statistically higher than the 1 year: at 1, 2, 4 days, p &amp;lt;0.05, on day 7 no differences were found (p = 0.34). A dynamic comparison of β-ARM indicators in the control group at the time of the index hospitalization and through 1 year revealed differences at all days early postinfarction period, p &amp;lt;0.05. In the control group, the β-ARM indicator reached normal values by 1-year follow-up period. In MINOCA patients, β-ARM indices after 1 year were statistically higher than in the control group, p = 0.008. Conclusions: The β - ARM indices in MINOCA patients after 1 year from the ischemic event are higher than in the control group. In dynamics, the β - ARM indices statistically decreased in the control group, but did’n change in MINOCA patients. Despite the use of a beta-blocker in MINOCA patients, increased SAS activity persists; therefore, β-APM values did’n change significantly after 1 year.

Dynamics of adreneractivity after transfer of myocardial infarction: annual observation

To study the change in the -adrenergic reactivity of red blood cell membranes in patients during the first year after acute myocardial infarction. The study included 25 patients with acute myocardial infarction (AMI) who signed informed consent to participate in the study. The erythrocyte membrane -adrenoreactivity index (-ARM) was determined in venous blood samples upon admission to the intensive care unit, one day after admission, 6 and 12 months after the index MI was transferred using the BETA-ARM-AGAT reagent kit (Agat-Med, Russia). According to the results of dynamics assessment of -APM during the first day, patients included in the study were divided into 2 groups. Group 1 (n=14) included patients who had an increase in -APM in the first day, and group 2 (n=21) included patients in whom -ARM either did not change or decreased. At the time of admission to the hospital in the formed groups, there were no differences in the -APM index and clinical and anamnestic characteristics. A day after hospitalization, the value of -APM in group 1 significantly exceeded the same indicator in group 2 (p=0.02). At the periods of 6 and 12 months, the -APM indices in the groups did not differ. In the 2nd group of patients, the progression of chronic heart failure to one or more functional classes (NYHA) was significantly more often compared with the 1st group. The study showed that on the first day in patients with AMI, both an increase and a decrease in the activity of the sympathoadrenal system are possible with a further leveling of these differences over the next year. For a group of patients with decreased activity of sympathoadrenal system on the first day, a more unfavorable course of heart failure in the post-infarction period is characteristic.

Studying fatty acid content in red blood cell membranes in diabetic retinopathy of type 2 diabetes patients

Introduction. Studying pathogenetic pathways that are formed under conditions of hyperglycaemia and trigger further metabolic changes resulting in diabetes complications over time are of special interest both in theoretical and practical medicine. Membrane of the red blood cell that stays in blood constantly and relatively durable is an obvious participant of all metabolic changes and cannot but reflect changes in metabolism. Objective: to study content of fatty acids (FAs) in red blood cell membranes of patients with diabetic retinopathy (DR) with type 2 diabetes (T2D). Materials and methods. The study enrolled 73 subjects maximally matched by age and gender: 50 patients with established long-lasting T2D complicated with DR and 23 patients in the control group. All the biochemical tests were performed at the certified laboratory under the standard methods. Gas chromatography was applied to study FA spectrum. Statistical analysis was performed in IBM SPSS Statistics 23. Results and discussion. The key difference between the lipid metabolism of patients with complicated long-lasting T2D and healthy subjects was a significant difference in FA content redistribution in the red blood cell membranes represented by increased “saturation”. The content of saturated FAs (SAFAs)in patients with DR was higher than in the CG: 1.5-fold for palmitic (С:16), 2-fold for myristic(С:14), pentadecanoic (С:15) and margaric (С:17) (Р &lt; 0.05). The content of saturated stearic (С:18) FA was not changed significantly.The content of unsaturated FAs(USFAs) have changed multidirectionally: the content of linoleic (С18:2) and arachidonic (С20:4) decreased 1.5-1.7-fold, respectively. The content of linolenic (С18:3) increased 2-fold, and content of oleic FA (С18:1) was not changed significantly. Conclusion. Study of the fluctuations of FA levels in red blood cell membranesplays animportant role in the development of insulin resistance, T2D and its microvascular complicationsthat can be applied in creating treatment and prevention strategies ofDRin type 2 diabetes patients.

Defects of Red Blood Cell Membranes in Patients with Brain Dysfunction (Pilot Study)

The aimof the paper: to identify promising diagnostic and prognostic biomarkers of pathological processes development based on the red blood cell membrane morphology and nanostructure in patients with brain disorders in the Intensive Care Unit.Materials and methods. The study included 24 patients from the anesthesiology and resuscitation ward of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology. Blood was acquired from the patients for standard tests, and all further tests were performed in vitro. The images of red blood cells were obtained using the atomic force microscope «NTEGRA Рrima» (NT-MDT, Russia) in semi-contact mode.Results. Patients from the anesthesiology and intensive care ward with traumatic brain injury, ischemic and hemorrhagic stroke, cerebral edema, and post-hypoxic encephalopathy had different blood cell shapes and localized defects of different topology on the surface of erythrocyte membranes including defects of pallor, torus, and nanostructure.Conclusion. In this pilot study we have shown that several defects represent the trigger mechanisms for the development of a total membrane damage. Local topographic defects of nanostructures and abnormalities of erythrocyte morphology are irreversible. The number and quality of these abnormalities may eventually be used as a diagnostic and prognostic biomarker of pathological processes.

Функциональное состояние клеточных мембран у больных мочекаменной болезнью

Функциональное состояние клеточных мембран у больных мочекаменной болезнью

Дослідження цитоплазматичних мембран клітин епітелію слизової оболонки носа у хворих на хронічний гнійний риносинуїт методом флуоресцентних зондів

Дослідження цитоплазматичних мембран клітин епітелію слизової оболонки носа у хворих на хронічний гнійний риносинуїт методом флуоресцентних зондів

Significant decrease of saturation index in erythrocytes membrane from subjects with non-alcoholic fatty liver disease (NAFLD)

BackgroundThe lipidomic profiling of erythrocyte membranes is expected to provide a peculiar scenario at molecular level of metabolic and nutritional pathways which may influence the lipid balance and the adaptation and homeostasis of the organism. Considering that lipid accumulation in the cell is important in promoting tissue inflammation, the purpose of this study is to analyze the fatty acid profile in red blood cell membranes of patients with Non-Alcoholic Fatty Liver Disease (NAFLD), in order to identify and validate membrane profiles possibly associated with the degree of hepatic damage.MethodsThis work presents data obtained at baseline from 101 subjects that participated to a nutritional trial (registration number: NCT02347696) enrolling consecutive subjects with NAFLD. Diagnosis of liver steatosis was performed by using vibration-controlled elastography implemented on FibroScan. Fatty acids, extracted from phospholipids of erythrocyte membranes, were quantified by gas chromatography method.ResultsThe subjects with severe NAFLD showed a significant decrease of the ratio of stearic acid to oleic acid (saturation index, SI) compared to controls, 1.281 ± 0.31 vs 1.5 ± 0.29, respectively. Low levels of SI in red blood cell membranes, inversely associated with degree of liver damage, suggest that an impairment of circulating cell membrane structure can reflect modifications that take place in the liver. Subjects with severe NAFLDalso showed higher levels of elongase 5 enzymatic activity, evaluated as vaccenic acid to palmitoleic acid ratio.ConclusionsStarting from these evidences, our findings show the importance of lipidomic approach in the diagnosis and the staging of NAFLD.

The state of erythrocyte cell membrane in patients with B-cell non-Hodgkin`s lymphoma

The state of erythrocyte cell membrane in patients with B-cell non-Hodgkin`s lymphoma

Cardiopulmonary syndrome and adrenoreactivity of an organism

Aim. To study β-adrenoreactivity of the cell membrane in patients with different variants of heart failure in association with chronic obstructive pulmonary disease.Methods. 120 heart failure patients including 68 of them who suffer from concominant chronic obstructive pulmonary disease were evaluated. Assessment of clinical features of heart failure, patients’ quality of life and study of β-adrenoreactivity were performed.Results. Adrenoreactivity of an organism in heart failure and concominant chronic obstructive pulmonary disease was 2 times higher and was 55.4±18.8 U and in heart failure only it was 29.4±8.5 U. Intensification of β-adrenoreactivity was found to be proportional to worsening of clinical features of chronic heart failure in all patients that was more prominent in patients with pulmonary disease. Responders with heart failure in association with chronic obstructive pulmonary disease had higher values of β-adrenoreactivity of cell membranes more frequently.Conclusion. The results of our investigation confirm increased activity of sympathetic system in heart failure and concominant chronic obstructive pulmonary disease that worsens clinical manifestations of heart failure.

SUN-PP038: Lipidomics Profile of Red Blood Cell Membrane in Patients on Long-Term Home Parenteral Nutrition: Effect of Two Different Lipid Emulsions

SUN-PP038: Lipidomics Profile of Red Blood Cell Membrane in Patients on Long-Term Home Parenteral Nutrition: Effect of Two Different Lipid Emulsions

IGFBP-3/transferrin/transferrin receptor 1 complexes as principal mediators of IGFBP-3 delivery to colon cells in non-cancer and cancer tissues

PurposeThe aim of this work was to study the involvement of IGFBP-3/Tf complexes in the pathology of colorectal carcinoma (CRC), quantify them, investigate their relation to iron concentration and binding to transferrin receptor (TfR) in colon tissue (non-cancer and cancer), and to assess the priority of this pathway for internalization of IGFBP-3. MethodsThe presence of IGFBP-3/Tf complexes was analyzed in sera from healthy persons and patients with CRC, and in colon tissue by immunoblotting. Complexes were immunoprecipitated, quantified by immunoassay and structurally characterized by immunoblotting, lectin blotting and mass spectrometry. Complexes which interacted with colon cells were immunoprecipitated with anti-TfR1 antibody and studied. Colon tissue slides were subjected to immunohistochemical analysis. ResultsThe concentration of IGFBP-3/Tf complexes was three times lower in patients with CRC. They were increasingly carbonylated, sialylated, contained more Galβ4GlcNAc units, expressed altered charge density and increased affinity for metal ions. Immunoprecipitation experiments revealed more TfR1 on membranes than in cytosol of colon cells, also more in cancer than non-cancer tissue. TfR1 on membranes were less occupied with IGFBP-3/Tf complexes than in cytosol. Immunofluorescent staining indicated a remarkable degree of co-localization of IGFBP-3 and TfR1, evenly distributed in non-cancer tissue and both evenly and cell surface concentrated in cancer tissue. ConclusionsIncreased expression of TfR1 on colon cell membranes in patients with CRC compensates for the reduced extracellular availability of IGFBP-3/Tf and TfR1 is the principal binding partner of extracellular IGFBP-3. IGFBP-3/Tf complexes in patients with CRC exhibit increased affinity for iron ions.

Protein and lipid composition of cell membranes in patients with bronchitis

Summary. We conducted a qualitative and quantitative analysis of erythrocyte cell membrane composition in patients with occupational bronchitis and in controls. Protein and lipid spectrum of erythrocyte membranes was investigated. The study involved 49 patients with occupational bronchitis and 31 healthy control persons. The study compared cell membrane proteins and phospholipids in patients suffering from bronchitis and in the control group. An excessive level of several cytoskeletal proteins and a significant deficit of fosfatidilinozitol / serin were found in patients with occupational bronchitis.

P03-138 Aberrant tyrosine transport - a biological marker for an inherited susceptibility to schizophrenia

A biological marker may serve as a link between the clinical manifestation of schizophrenia and the underlying susceptibility gene (s) and it may also serve the purpose to delineate subgroups or “endophenotypes”. Aberrant tyrosine transport across the cell membrane in patients with schizophrenia is such a biological marker. Previous studies have showed a reduced tyrosine transport across the fibroblast cell membrane in patients with schizophrenia. the experiments were performed in fibrobasts that had grown for several generations in vitro. Thus, the aberrant tyrosine transportation is likely to reflect a genetic trait that is transmitted through several cell generations. an association between aberrant tyrosine transport across fibroblast membrane and neurocognitive dysfunction was found for patients with schizophrenia in a previous study indicating a connection between deficient tyrosine transport and cognitive dysfunction. the aim of the present study was to investigate the occurrence of aberrant tyrosine transport across the fibroblast membrane in first-degree relatives to patients with schizophrenia to find indications of inheritance. Significant correlations between patients and their mothers were found regarding aberrant tyrosine transport and, furthermore, the mothers with aberrant tyrosine transport showd signs of reduced cognitive functioning. Maternal inheritance is a possible mode for genetic transmissionssion of susceptibility to schizophrenia for a subgroup of patients with low cognitive functioning.

Effects of type 2 diabetes mellitus on plasma fatty acid composition and cholesterol content of erythrocyte and leukocyte membranes

Insulin resistance is a major factor in the pathogenesis of type 2 diabetes mellitus (T2DM) and is related to the fatty acid profile of the plasma membranes. The purpose of the present study was to investigate fatty acid composition and cholesterol content of cell membranes in patients with type 2 diabetes and, thus, to evaluate the possible factors leading to the alteration of plasma membrane fluidity. The study was performed in 20 healthy control subjects and 32 patients with type 2 diabetes. The fatty acid profiles and cholesterol content of the erythrocyte (RBC) and leukocyte (WBC) membranes were determined by a gas chromatographic method. When one considers the membrane constituents increasing fluidity and the ones decreasing it, the diabetics had a membrane composition decreasing fluidity compared to controls. On the other hand, when compared to control subjects, type 2 diabetic patients showed a significantly higher proportion of C16:0 components in erythrocyte and leukocyte membranes and plasma samples (25.4+/-3.1% vs. 31.1+/-4%; 23.3+/-2.4% vs. 29.3+/-5.2%; 27.6+/-3.9% vs. 34.5+/-5.7%; p<0.005, p<0.01 and p<0.005, respectively). Our results suggest that the ratio of saturated:unsaturated fatty acids changes in plasma and cell membranes of patients with type 2 diabetes. This situation may cause, at least in part, RBC-WBC function abnormalities and insulin resistance because of inconvenient membrane fluidity.

Alterations in calcium and magnesium content of red cell membranes in patients with primary hypertension

A cellular calcium–magnesium antagonism seems to be involved in the pathogenesis of primary hypertension. Total plasma, intracellular, and membranous calcium (Ca) and magnesium (Mg) contents were determined in 39 untreated patients with essential hypertension (EH) and 40 normotensive healthy subjects (NT). Membranous and intracellular measurements were performed in erythrocytes. Ca and Mg contents were measured by atomic absorption spectroscopy and membrane protein was determined according to Bradford’s method as a membranous reference. There was no significant difference in plasma Ca (NT: 2.60 ± 0.15 v EH: 2.64 ± 0.17 mmol/L) and Mg concentrations (NT: 0.83 ± 0.12 v EH: 0.87 ± 0.14 mmol/L) in the studied groups. Intracellular Mg (NT: 1.72 ± 0.15 mmol/L v EH: 1.64 ± 0.19 mmol/L) and Ca (NT: 2.06 ± 0.20 mmol/L v EH: 2.10 ± 0.24 mmol/L) contents were also not significantly different between groups. Membrane Ca content was significantly increased in the EH group (2.23 ± 0.32 μmol/g membranous protein) compared to controls (1.05 ± 0.30 μmol/g membranous protein, P < .01). On the contrary, membranous Mg content was significantly decreased compared to controls (0.31 ± 0.09 v 0.50 ± 0.10 mmol/g membranous protein content, P < .01). The Ca/Mg ratio in membranes was significantly increased in EH as compared to healthy subjects ( P < .01) and correlated to mean arterial blood pressure values (r = 0.47, P < .01). We conclude that the membranous alterations of Ca and Mg metabolism, shown by increased Ca/Mg ratio in red cell membranes of hypertensive subjects, may play a role in the pathogenesis of primary hypertension.

Increased Expression of Neutrophil and Monocyte Adhesion Molecules LFA-1 and Mac-1 and Their Ligand ICAM-1 and VLA-4 Throughout the Acute Phase of Myocardial Infarction: Possible Implications for Leukocyte Aggregation and Microvascular Plugging

Objectives. This study sought to evaluate expression of adhesion molecules on neutrophils and monocytes throughout the acute phase of myocardial infarction.Background. Neutrophil and monocyte counts increase within days from onset of acute myocardial infarction. Because leukocytes are recruited to the involved myocardial region, we postulated that these activated cells would display an increased expression of adhesion molecules necessary for effective endothelial transmigration.Methods. We measured the expression of neutrophil and monocyte lymphocyte function associated antigen-1 (LFA-1), Mac-1, very late after activation antigen-4 (VLA-4) and intercellular adhesion molecule-1 (ICAM-1) by flow cytometry throughout the acute phase of acute myocardial infarction in 25 patients and 10 age-matched control subjects.Results. Expression of Mac-1 on neutrophils increased significantly, whereas no expression of VLA-4 and ICAM-1 was detected. The expression of LFA-1, Mac-1, VLA-4 and ICAM-1 on the monocyte cell membrane in patients with an acute myocardial infarction was increased compared with that in control subjects by 22% (on day 7), 67%, 13% and 44% (all on day 4), respectively (all p < 0.001). Elevated density of monocyte-specific CD14 in the AMI versus the control group was also shown (30%, p < 0.001).Conclusions. Increased expression of neutrophil and monocyte adhesion molecules may contribute to their adhesion to endothelium in the ischemic territory. This adhesion could feasibly precipitate vasoconstriction or add a local thrombotic effect due to tissue factor expression secondary to Mac-1 engagement. In addition, the manifestation of increased density of LFA-1 and Mac-1 by activated leukocytes with monocytes also expressing ICAM-1 suggests that leukocytes may form microaggregates that could cause microvascular plugging. This mechanism may facilitate the occurrence of the “no-reflow” phenomenon or slow coronary filling after acute myocardial infarction.

THE STRUCTURAL AND FUNCTIONAL STATE OF RED CELLS MEMBRANES IN CHILDREN WITH ACQUIRED APLASTIC ANEMIAS AND MYELODYSPLASTIC SYNDROME EXPOSED TO THE ACTION OF LOW-DOSES RADIATION. 154

From 1986 to 1996 53 children (age 1-15 years old) with acquired aplastic anemia (AAA) and myelodysplastic syndrome were followed. All children lived in the regions with chronic action of low doses radiation. The biophysical indexes of erythrocyte membranes (microviscosity of lipid bilayer (MLB), osmotic and thermostability, Mt-Hb level, daily hemolysis (DH), RBC lifespan) were investigated. It had been shown that red cells of children with AAA and MDS obtained improved osmotic resistance. There are some differences between kinetic values of red cell's thermohemolysis in children with AAA and MDS. The decrease of thermoresistance of red cell membrane in patient with AAA and MDS could be explained by changes in Hb values. In comparison with control group average level of Mt-Hb in red cells was elevated by 5-10%. Besides, the descrease of red cell thermoresistancy was associated with the disturbances of lipid-lipid interaction in membranes. It was revealed that kinetic values of membrane acetylcholinesterase were low in children with AAA and had no significant differences in children with MDS. There was an increase of MLB in red cell membrane in patients with AAA and no changes of the latter in patients with MDS. Kinetics of red cell revealed intensification of DH and short-term RBC lifespan in patients with AAA and MDS. Our data showed that red cell membranes are sensitive markers and take part in pathogenesis of AAA and MDS.

Changes in red blood cell membrane structure in patients with chronic renal failure.

The properties of red blood cell membranes in patients with chronic renal failure were investigated using electron paramagnetic resonance spectroscopy. Using spin traps, 5,5-dimethylpirroline-1 oxide and N-tert-butyl-alpha-phenylnitrone, we found generation of hydroxyl radicals in the blood of patients with chronic renal failure after 20 min of regular hemodialysis. The physical state of membrane proteins and membrane osmotic fragility and reductive properties of red blood cells were studied. The increase in the relative correlation time of 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidine-1 oxyl indicates the immobilization of membrane protein molecules in erythrocytes of chronic renal failure patients. The decrease in membrane protein mobility was observed in whole blood incubated with tert-butylhydroperoxide, regardless of the presence of iron. We found that the addition of ferrous ions did not aggravate profound changes in membrane proteins induced with tert-butylhydroperoxide. We also demonstrated higher osmotic fragility of erythrocytes in the patients with renal failure as compared to normal subjects. These alterations in membrane structure of red blood cells in hemodialysed patients suggest that hydroxyl radicals generated during hemodialysis can play an important role in the oxidative mechanism of erythrocyte damage.