Cell-free Supernatant Fractions Research Articles

The glyoxylate cycle as a stage in the conversion of fat to carbohydrate in castor beans

1. 1. Cell-free supernatant fractions of castor beans catalysed the following reactions: 2. (a) the aldol fission of isocitrate, to produce equal amounts of glyoxylate and succinate; 3. (b) the activation of acetate in the presence of CoA and ATP; 4. (c) the condensation of acetyl CoA with oxaloacetate, to form citrate, and with glyoxylate, to form malate; 5. (d) the overall formation of malate from acetyl CoA and either citrate or isocitrate. 6. 2. These reactions of the glyoxylate cycle, when coupled with the reactions leading from fat to acetyl CoA, and from malate to carbohydrate, provide a route for the net conversion of fat to carbohydrate.