Drug-eluting coronary stents mediate inflammation-associated protein signature in an experimental in vitro study.

Dihydrotanshinone I inhibits ovarian tumor growth by suppressing ITGB1/FAK-mediated extracellular matrix signaling.

Zebrafish CD44a variants suppress antiviral immunity through regulation of RIG-I ubiquitination and degradation.

In situ piezoelectricity induces M2 polarization of macrophages to regulate Schwann cells for alleviating neuropathic pain of CCI rats.

Dysregulation of SUSD2-CLDN18.2-mediated cell adhesion contributes to lung adenocarcinoma progression associated with chronic low-dose nanoplastics exposure.

The kidney slit diaphragm resembles a fishnet.

ABSTRACTBackgroundCognitive impairment is one of the main complications after a stroke and seriously affects the quality of life and survival time of patients, thereby causing a heavy burden on the social economy and public health. Although exercise is an effective non‐pharmacological strategy for prevention and treatment of cognitive impairment, the mechanism(s) of this effect remain unclear.MethodsThe current study investigated the effects of irisin treatment on the behavioral characteristics of mice with post‐stroke cognitive impairment (PSCI). The expression levels of platelet endothelial cell adhesion molecule 1 (PECAM, PECAM‐1, CD31), glial fibrillary acidic protein (GFAP), vascular endothelial growth factor (VEGF), and molecules in the adenosine 5‐monophosphate (AMP)‐activated protein kinase (AMPK)–endothelial nitric oxide synthase (eNOS) signaling cascade in the hippocampus were then measured.ResultsIrisin significantly enhances learning and memory functions in cases of PSCI. This improvement correlates with a reduction in cerebral infarction size and decreased neuronal death. Additionally, irisin treatment resulted in a marked decrease in the levels of astrocytic scar formation in the cortex. Furthermore, irisin activates the AMPK‐eNOS signaling pathway, which promotes the expression of VEGF. The irisin compounds are involved in the process of brain angiogenesis and play a critical role in endothelial and reactive astrocytes function.ConclusionThe study revealed a potential mechanism by which exercise‐induced irisin secretion may attenuate PSCI. Irisin improved endothelial dysfunction and neuroinflammation, suggesting it may be a promising target for PSCI therapy.

Previous studies found that melanoma antigen genes (MAGE) were antigens expressed in various tumor cells but hardly expressed in normal tissues, and their unique expression pattern made them highly promising for cancer immunotherapy. MAGE-A10 was a member of the MAGE family, and although it was expressed in various tumors, its specific function remained unclear. In this study, we conducted a comprehensive pan-cancer analysis of MAGE-A10 expression, prognostic value, DNA methylation, genetic variation, function, immune infiltration, and drug sensitivity using multiple public databases. The results showed that MAGE-A10 was highly expressed in tumor tissues of most cancer types and was associated with poor prognosis. Additionally, MAGE-A10 was closely related to methylation levels, genetic variation, immune cell infiltration, immune therapy response, and chemotherapy resistance, possibly due to its role in regulating gene expression, cell differentiation, and immune response. Validation experiments in gastric cancer found that high expression of MAGE-A10 significantly affected patient prognosis. Gene set enrichment analysis (GSEA) indicated that high expression of MAGE-A10 was closely associated with the binding of histone deacetylases. Cell experiments showed that knocking down MAGE-A10 significantly reduced the proliferation, migration, and invasion capabilities of gastric cancer cells, which might be related to its regulation of the expression of cell adhesion molecule cadherins. In conclusion, this study indicated that MAGE-A10 was a potential tumor prognostic biomarker and immunotherapy target and played an important role in the proliferation, migration, and invasion of gastric cancer cells.Supplementary InformationThe online version contains supplementary material available at 10.1038/s41598-025-17987-y.

Effectiveness of circulating tumor cells and circulating tumor DNA in peritoneal lavage fluid for predicting metachronous peritoneal metastasis of gastric cancer.

Genetic compensation highlights the importance of neural cell adhesion molecule Ncam1 paralogs in balancing signaling pathways during zebrafish lateral line development.

Simple SummaryRed claw crayfish (Cherax quadricarinatus) is an important aquatic species, but its growth and health can be affected by poor water conditions and diseases in intensive farming. To address this, we studied whether adding Rhodotorula mucilaginosa to their feed could help. We fed crayfish different amounts of this yeast (0, 0.1, 1.0, and 10.0 g/kg of feed) for 56 days and observed their growth, digestion, immunity, and gut health. The results showed that adding the yeast improved the crayfish’s growth. It also helped them digest food better by increasing the activity of digestive enzymes. The yeast boosted their ability to fight off diseases by enhancing immune-related functions and reduced harmful bacteria in their guts while increasing beneficial ones. The best effect was seen when adding 1.0 g/kg of yeast to the feed. These findings can help farmers raise healthier crayfish more efficiently, supporting sustainable aquaculture and providing a safer food source for people.This study investigated the effects of dietary Rhodotorula mucilaginosa supplementation with different concentrations (0.0 g/kg, 0.1 g/kg, 1.0 g/kg, 10.0 g/kg) on red claw crayfish (Cherax quadricarinatus). Four groups were established: control group (CK, 0.0 g/kg), low-dose group (HL, 0.1 g/kg), medium-dose group (HM, 1.0 g/kg), and high-dose group (HH, 10.0 g/kg). The feeding trial lasted for 56 days. The results showed that, compared with the control group, all supplementation groups exhibited significantly reduced feed conversion ratios (p < 0.05). The HM and HH groups demonstrated significant increases in body length growth rate, specific growth rate, weight gain rate, hepatosomatic index, and survival rate (p < 0.05). All supplemented groups showed significantly enhanced trypsin and lipase activities in intestines and trypsin activity in the hepatopancreas (p < 0.05). The HM and HH groups exhibited elevated α-amylase activity in the hepatopancreas (p < 0.05). Compared with the control group, marine red yeast supplementation reduced colonization of potential pathogens while increasing probiotic abundance, effectively improving intestinal microbiota structure. The HM group significantly improved intestinal villus length, width, and muscular thickness (p < 0.05). All supplemented groups showed considerable upregulation of hepatopancreatic genes related to immunity (heat shock protein 70, down syndrome cell adhesion molecule, crustacean antibacterial peptide, serine proteinase inhibitors, crustacean hyperglycemic hormone, anti-lipopolysaccharide factor, lysozyme, and alkaline phosphatase) and antioxidant defense (superoxide dismutase, glutathione peroxidase, glutathione, and catalase) (p < 0.05). These findings indicate that R. mucilaginosa can significantly enhance digestive enzyme activity, maintain intestinal health, improve antioxidant and immune-related gene expression, and promote growth performance in red claw crayfish, with the HM group (1.0 g/kg R. mucilaginosa) showing optimal promotion effects.

Efficacy of Yangxue Zhiyang decoction in treating neurodermatitis and its impact on the expression levels of cell adhesion molecules intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1

Therapeutic potential of TIM-3 inhibition in cancer, viral infections, and autoimmune disorders.

Targeting the VIP-VPAC Pathway in Melanoma Models Inhibits Tumor Growth and Liver Metastasis.

ABSTRACTAcral melanoma (AM) is a rare subtype of cutaneous melanoma mainly found in acral locations. The treatment of advanced AM remains challenging due to its rarity and the distinct features of this subtype compared with the other common types of melanomas. There is thus an urgent need to develop effective therapeutic approaches for AM. This study was established to screen and evaluate potential therapeutic targets for AM. DNA microarray analysis comparing normal epidermal melanocytes and AM cell lines (SM2‐1 and MMG‐1) showed that approximately 500 genes were highly expressed in the AM cell lines compared with the levels in normal melanocytes. Among them, melanoma cell adhesion molecule (MCAM) was selected for further analyses and was found to be significantly highly expressed in AM cell lines compared with the levels in melanocytes and keratinocytes. Knockdown of MCAM significantly inhibited the proliferation of AM cell lines with decreased expression of cyclin D1 and BCL2. The cytotoxicity of MCAM‐targeted antibody‐drug conjugate was further evaluated and it significantly decreased the viability of AM cell lines. In conclusion, MCAM is highly expressed in AM cell lines and affects their proliferation, likely through modulating the expression of cyclin D1 and BCL2. These findings highlight the potential of MCAM as a therapeutic target of AM.

Obesity promotes conserved inflammatory and metabolic transcriptional programs in colon tumors: evidence from mouse models and the ColoCare Study Patient Cohort.

CEACAM7 enhances oral cancer metastasis by upregulating CD317 expression.

Development of an anti-CDH15/M-cadherin monoclonal antibody Ca15Mab-1 for flow cytometry, immunoblotting, and immunohistochemistry.

ELFN1 deficiency: The mechanistic basis and phenotypic spectrum of a neurodevelopmental disorder with epilepsy.

Differential effects of antiretroviral HIV integrase inhibitors on vascular cell adhesion molecules.