Celery Plants Research Articles

AgZDS, a gene encoding ζ-carotene desaturase, increases lutein and β-carotene contents in transgenic Arabidopsis and celery

ζ-Carotene desaturase (ZDS) is one of the key enzymes regulating carotenoids biosynthesis and accumulation. Celery transgenic efficiency is low and it is difficult to obtain transgenic plants. The study on ZDS was limited in celery. Here, the AgZDS gene was cloned from celery and overexpressed in Arabidopsis thaliana and celery to verify its function. The AgZDS has typical characteristic of ZDS protein and is highly conserved in higher plants. Phylogenetic analysis showed that AgZDS has the closest evolutionary relationship with ZDSs from Solanum lycopersicum, Capsicum annuum and Tagetes erecta. Overexpression of AgZDS gene in A. thaliana and celery resulted in increased accumulations of lutein and β-carotene and up-regulated the expression levels of the genes involved in carotenoids biosynthesis. The contents of lutein and β-carotene in two lines, AtL1 and AgL5, were the highest in transgenic A. thaliana and celery, respectively. The relative expression levels of 5 genes (AtPDS, AtZISO, AtZEP, AtNCED3, and AtCCD4) were up-regulated compared to the wild type plants. The relative expression levels of most genes in carotenoids biosynthesis pathway, such as AgPDS, AgCRTISO1, and AgZISO, were up-regulated in transgenic celery plants. The antioxidant capacity of A. thaliana and photosynthetic capacity of celery were also enhanced. This research is the first report on the function of structure gene related to carotenoid biosynthesis in transgenic celery plants. The findings in this study demonstrated the roles of AgZDS in regulating carotenoids metabolism of celery, which laid a potential foundation for quality improvement of celery.

The Production of Compost from Organic Wastes using Bioactivators and Its Application to Celery (Apium graveolens L.) Plant

Dilakukan pembuatan pupuk kompos dari limbah Jerami padi sawah percobaan dan seresah daun tanaman buah-buahan kebun plasma nutfah Cibinong Science Center-Botanic Garden (CSC-BG) LIPI menggunakan bioaktivator StarTmik@OK dan Tricho Plus (LIPI) dan Promi (produk komersial). Kompos yang dihasilkan diaplikasikan kepada tanaman seledri (Apium graveolens L.) di rumah kasa (screenhouse) Puslit Bioteknologi-LIPI Cibinong, Kabupaten Bogor, Jawa Barat. Parameter pertumbuhan yang diamati adalah tinggi tanaman, jumlah petiol daun, dan biomassa. Penelitian disusun berdasarkan Rancangan Acak Lengkap (RAL) dengan tiga ulangan. Data hasil penelitian dianalisis secara statistik menggunakan SPSS ver. 24 dilanjutkan dengan uji jarak berganda Duncan (DMRT). Hasil menunjukkan bahwa secara umum kompos yang berasal dari jerami padi dengan menggunakan berbagai bioaktivator menghasilkan nilai rataan pertumbuhan tertinggi yaitu tinggi tanaman (35,7 cm), jumlah petiol (23,7), dan biomassa (42,3 g) dibandingkan dengan kompos yang menggunakan bahan lainnya. Penggunaan pupuk kimia masih diperlukan untuk meningkatkan pertumbuhan tanaman seledri.Abstract The organic waste of rice straw from the experimental paddy field and broadleaf litter from the fruit germplasm garden of Cibinong Science Center-Botanic Garden (CSC-BG) was processed into compost with the addition of bioactivators. The bioactivator of StarTmik@OK and Tricho Plus (LIPI) and Promi (commercial product) was added to the composting raw materials. Produced compost was then applied to celery (Apium graveolens L.) plants in the screenhouse of RD Center for Biotechnology-LIPI. The growth parameters observed were plant height, leaf petiole, and biomass. The experiment was arranged in a Completely Randomized Design (CRD) with three replications. Obtained data were statistically analyzed using SPSS ver.24, followed by Duncan’s multiple range tests (DMRT). The result showed that the highest growth rates, i.e., plant height (35.7 cm), number of petiol (23.7), dan biomass (42.3 g), were obtained by using rice straw compost using various bioactivators. Meanwhile, chemical fertilizer is still needed to increase the growth of celery plants.

Pengaruh Penggunaan Limbah Air Cucian Beras terhadap Pertumbuhan Tanaman Seledri (Apium graveolens L.) dengan Sistem Penanaman Hidroponik

Rice washing water waste is the result of community waste which in general is no longer used, even though the waste can still be processed and used, one of which is as fertilizer for plants. Therefore, this study aims to determine the effect of the use of rice washing water waste on increasing the growth of celery (Apium graveolens L.) with a hydroponic planting system. The research location is in the Laboratory and Green House of the Biology Education Study Program, FKIP, Tadulako University. The study was carried out experimentally with a completely randomized design (CRD), which consisted of 4 types of treatment and was repeated 4 times. The treatments consisted of: K0 (control) = 0% without giving rice washing water; K1 = 25% of rice washing water waste; K2= 50% of rice washing water waste; and K3 = 75% rice washing water waste. The data obtained were then analyzed using the ANOVA test and a further BNT test was carried out which was processed using the SPSS 25 program. The results showed that the height of celery plants at the age of 10 DAP (Days After Planting), 20 DAP, and 30 DAP, was significantly affected by the treatment. rice washing water waste, although the concentrations were not significantly different between treatments. These results indicate that rice washing water has a significant effect on increasing the growth of celery plants.

Responses of Leaf Celery to Floating Culture System with Different Depths of Water-substrate Interface and NPK-fertilizer Application

Wetland areas in Indonesia cover more than 33,3 million hectares, and slightly less than 40 % is inland swamp. During the rainy season, for up to 9 months annually, the wetlands are flooded, and no conventional agricultural activities can be done by local farmers. However, this condition can be seen as an opportunity to employ floating culture system. The objective of this research was to evaluate responses of leaf celery to floating culture system with different depths of water-substrate interface and NPK-fertilizer application. The results of this study indicated that the depth of water-substrate interface (WSI) should be maintained between 1 to 3 cm. At less than 1 cm, continuous contact between the water surface and the bottom part of the substrate cannot be ensured; meanwhile, aerobic substrate volume was reduced and caused significant effects on growth and yield in celery plants if WSI was deeper than 3 cm. Moreover, the effectiveness of NPK-fertilizer application was weakened if the depth of WSI was at 6 cm. Fresh leaf yield in celery plants harvested at 45 days after transplanting (DAT) can be predicted as early as 3 weeks earlier using the midrib length of the largest leaf or plant height as a predictor measured at 26 DAT.
 HIGHLIGHTS
 
 Depth of water-substrate interface (WSI) should be maintained between 1 to 3 cm for better growth and higher yield in floating culture system
 Effects of NPK fertilizer application diminished if WSI deeper than 3 cm
 Yield of celery harvested at 45 days after transplanting can be predicted as earlier as 3 weeks using midrib length of the largest leaf or plant height as predictor
 
 GRAPHICAL ABSTRACT

RESPONS PERTUMBUHAN DAN HASIL TANAMAN SELEDRI (APIUM GRAVEOLENS L.) TERHADAP MEDIA TANAM DAN KONSENTRASI LARUTAN NUTRISI YANG BERBEDA PADA WICK SYSTEM

This research aims to find out 1) the interaction between the medium of planting and the concentration of nutrient solutions to the growth and yield of celery plants hydroponically. 2) types of planting media that can give different influences on the growth and yield of celery plants, and 3) concentration of nutrient solution that provides the best growth and yield of celery plants in different planting media.The research was conducted from December 2019 to February 2020 at the Integrated Laboratory of the Faculty of Agriculture of Mulawarman University, Samarinda. The research used factorial exsperiment 3×4 on Randomized Completely Block Design (RCBD), replicated three times with each treatment consisting of three plants. The first factor is the planting medium consisting of rockwool, charcoal husk, and combination of charcoal husk + tea amps. The second factor is the concentration of AB Mix nutrient solution consists of 500, 1,000, 1,500, and 2,000 ppm. The data was analyzed with test F and continued Least Significant Difference (LSD) test with a level of 5%. The results showed that there is an interaction between planting media and concentration of nutrient solution in the growth and yield of celery plants. The interaction between the combination of charcoal planting media + tea pulp and concentration of 1,500 ppm is not real with the interaction of rockwool planting media and concentration of 1,500 ppm. From various types of planting media used, charcoal husk planting media provides the best influence on the growth and yield of celery plants. Based on polynomial orthogonal test the concentration of nutrient solution with an optimum point of 1,460.84 ppm affects the growth and yield of celery plants with a maximum dry weight of 2.14 g.

REPELLENCY OF CELERY ESSENTIAL OILS (Apium graveolens L.) AGAINST Spodoptera frugiperda (Lepidoptera: Noctuidae) IN THE LABORATORY

Repellency of celery essential oil (Apium graveolens L.) against Spodoptera frugiperda (Lepidoptera: Noctuidae) in thelaboratory. Essential oil is one of the ingredients that has repellent ability to herbivorous insects. Celery is one of plantscontaining essential oil which may also have the repellent ability. This study was aimed to determine the differences in therepellent ability of celery essential oils compounds that were taken from 3 different locations against S. frugiperda in thelaboratory. This research was arranged in a factorial completely randomized design (CRD) with two factors. The first factorwas the origin of the celery plants (Malang, Surabaya, and Lamongan) and the second factor was the concentration level ofessential oils (1000, 2000, and 3000 ppm) which was repeated three times. The reliability test was carried out using filter papermethod. The results showed that the essential oil extracted from celery originated from Malang with 3000 ppm concentrationhad the highest percentage level of repellency (level 5) that reached 93.33% of repellency. Location and concentration factorshad no different effect on S. frugiperda larvae repellency. There was no correlation between location and concentration on therepellency of S. frugiperda larvae.

Cultivation and Farming Analysis of Red Chili Intercropping with Celery

Intercropping is an alternative that should be developed especially to make maximum use of the land. Aromatic plants such as celery contain essential oils which, among other things, act as pest repellents. Therefore, celery plants can be used as a way of controlling pests by intercropping with chili plants as the main crop. This study aimed to determine the cultivation technology and analysis of the intercropping farming of red chilies with celery at the farmer level. The research was conducted at the Mekar Sari Farmer Group, Paal Merah Village, Paal Merah District, Jambi City, Jambi Province in October 2018. The data collected included primary data in the form of information from farmers as well as secondary data and conditions of the research area, social and economic potential. Primary data were collected using survey techniques, namely interviews with farmers and key information using a questionnaire. Samples were randomly assigned to a population of 15 farmer cooperator farmers who were intercropping red chili and celery at the study site. The data analysis technique included tabulation analysis which was used to understand the farmers' financial farming conditions, and the economic feasibility analysis uses the R/C ratio. The results of the research on the intercropping of red chili and celery, without considering the labor costs in the family, showed that this business was profitable with a total profit of 20,553,500,-IDR This farming has benefited from the production of celery as much as 405 kg, production of red chilies as much as 585 kg, and BEP prices of 15,495,-IDR/kg of celery and 14,143,-IDR/kg of red chilies. The R/C ratio value of 2.

Gas Chromatography- Mass Spectrometry Analysis of Phthalide Composition of Celery oil from Apium graveolens L. cultivated in Nigeria.

Phthalides are important bioactive constituents of celery plants. The medicinal uses of celery and their characteristic aroma have been linked to the phthalides. In this report, the leaf and stalk oils of celery were extracted either by hydrodistillation or maceration in water and the phthalide constituents of the oils were characterized by gas chromatography-mass spectrometry (GC-MS) analysis. The leaf oil consists mainly of dihydroxyphthalides while the volatile phthalides are the main phthalides in the stalk oil. The phthalide content of the leaf oil and the stalk oil are 39.8% and 29.95% respectively. The phthalides identified in the leaf oil are the isomeric compounds of senkyunolide I and senkyunolides (J & N), while the major phthalide constituents of the stalk oil include 3-n- butylphthalide, sedanolide, neocnidilide and sedanenolide/senkyunolide A. The results suggest that the Nigerian celery plant could be considered a good source of phthalides which are known for their nutraceutical properties such as anti-oxidant, antitumor, anti- platelet aggregation, hypotensive, hypoglycemic and hypolipidemic properties. Further phytochemical and pharmacological evaluation of the locally cultivated celery are required in order to exploit the medicinal potentials of the plant for applications in pharmaceutical and cosmetic industries.

Pengaruh Pemberian Pupuk Bokashi Terhadap Pertumbuhan dan Produksi Tanaman Seledri (Apium Graveolens L.)

<p class="abstrak">wet weight, but did not have a significant effect on the number of celery stems (<em>Apium </em>and Celery <em>(Apium graveolens</em> L.) is a vegetable plant that has very bright prospects. Every year the population that continues to increase causes the need for vegetables to also increase. So to meet the needs of consumers celery production needs to be increased. Therefore, it is necessary to look for alternative efforts to increase the yield of celery cultivation, one of which is the application of Bokashi fertilizer. Bokashi is the result of fermentation of organic ingredients with EM4 technology. The purpose of this study was to determine the effect of giving Bokashi on the growth and yield of celery (<em>Apium graveolens</em> L.) and to determine at what dose the application of Bokashi fertilizer on growth and production of celery plants (<em>Apium graveolens</em> L.). The research was conducted at Jalan Sempurna Pasar 7 Termbung and the wet weight of celery was carried out at the Biology laboratory, Faculty of Science and Technology, State Islamic University of North Sumatra, Medan in July 2019 - November 2020. The data obtained were tabulated and analyzed using the One Way Analysis of Variance test. (ANOVA) at the real level (significant) used, namely α = 0.05 and continued with the Duncan's Multiple Range Test (DMRT) to see the significant results between treatments. Data analysis was using the software statistical Product and Service Solutions (SPSS) version 23. The results showed that bokashi fertilizer had a significant effect on plant height and production of celery (<em>Apium graveolens</em> L.) is the D4 treatment with a dose of 40 grams.<em> graveolens</em> L. ). 5.1.2 The optimum dose of bokashi fertilizer which has a significant effect on the growth</p>

First Report of Alternaria tenuissimain Causing Leaf Spot of Celery (Apium graveolens) in China.

Celery (Apium graveolens) is one of the most widely grown vegetables in the world. A survey in Anding District of Gansu Province in 2019 showed that the incidence of celery leaf spot was 25%-45%. The disease mainly occurs in late June and July. The leaf spot is conducive to the onset at high temperature and humidity environment. The initial symptoms were many small light brown, irregular-shaped on the leaves. The lesions gradually enlarged in the later stage of the disease, and multiple lesions coalesced to form large irregular brown spots, eventually the whole leaves died. A 3~4mm leaf tissue was cut from the junction of the diseased leaf and the healthy area, the leaf tisse was surface-sterilized in 1.5% NaClO for 1 min and washed with sterile water. Then, it was incubated on potato dextrose agar (PDA) and obtained the pure culture (Q1). After 5 days of cultivation at 25°C, the fungal colonies were olivaceous to dark olive with white margins and abundant aerial mycelia. The conidia were obclavate or ellipsoid, pale brown, with 3~4 longitudinal septa and 2~7 transverse septa, and measured 20.0 to 50.0 × 3.5 to 14.0μm (n=50). Conidiophores were septate, arising singly, and measured 3.5 to 40.0 × 2.5 to 4.5 μm (n=50). Based on morphological characteristics, the fungus was preliminarily identified as A.tenuissima (Simmons 2007). To further confirm the identification, the internal transcribed spacer region (ITS), translation elongation factor 1-α gene (TEF), RNA polymerase II second largest subunit (RPB2), major allergen Alt a 1 gene (Alt a 1), endopolygalacturonase gene (endoPG), anonymous gene region (OPA10-2) and glyceraldehyde 3-phos-phatedehydrogenase (GAPDH) were amplified and sequenced using primers ITS1/ITS4 (Peever et al. 2004), EF1-728F/EF1-986R (Carbone et al. 1999), RPB2-5F2/RPB2-5R (Sung et al. 2007), Alt-for/Alt-rev (Hong et al. 2005), EPG-specific/EPG-3b (Peever et al. 2004), OPA10-2R/OPA10-2L (Peever et al. 2004) and Gpd1/Gpd2 (Berbee et al. 1999) (GenBank accession no.MN046364, MW016001, MW016002, MW016003, MW016004, MW016005, MW016006). DNA sequences of TEF, RPB2, endoPG, OPA10-2 and GAPDH were 100% identical to those of A. tenuissima (MN256108, MK605866, KP789503, JQ859829 and MK683802), but ITS and Alt a 1 were 100% similarity with A. tenuissima (MN615420, JQ282277) and A. alternate (MT626589, KP123847). The ITS and Alt a 1 sequence did not distinguish A. tenuissima from the A. alternate complex. Maximum likelihood phylogenetic analyses were performed for the combined data set with TEF, RPB2, and endoPG using MEGA6 under the Tamura-Nei model (Kumar et al. 2016). The isolate Q1 clustered with type strain A. tenuissima CBS 918.96. The 20 celery plants of 4-7 leaf age were used test the pathogenicity of Q1, the ten plants were sprayed with 20ml of spore suspension (1×105 spores/ml), the control was sprayed with 20mL sterile water, which were placed in a growth chamber (25℃, a 14h light and 10h dark period, RH > 80%). Eight days after inoculation, 40% of the leaves formed lesions, which were consistent with the field observation，the control group was asymptomatic. The pathogen was reisolated from infected leaves to fulfill Koch's postulates. To our knowledge, this is the first report of A. tenuissima causing leaf spot on celery in China.

Molekularna karakterizacija 'Candidatus Phytoplasma solani' na celeru - studija u Futogu

'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.

Scirtothrips dorsalis (Thysanoptera: Thripidae) Is a Pest of Celery, Apium graveolens (Apiales: Apiaceae): First Report and Diagnostic Characters

Abstract Scirtothrips dorsalis (Hood), the chilli thrips, is reported for the first time as a pest of celery, Apium graveolens L. Infested celery plants in a polyhouse at Kalahalli village (13°05′46.0′′N, 77°47′12.2′′E) in Hoskote taluk of Bengaluru Rural district, Karnataka, India, had light to dark brownish scars on various parts besides discolored and distorted leaves. Association of fungal or bacterial disease was dismissed based on microscopic analysis of tissue sections. Eggs, juveniles, and adults of thrips were abundant, especially on the leaves. Diagnostic characters of the adult S. dorsalis collected on celery are presented here along with photomicrographs. Juvenile and adult stages of S. dorsalis were also recorded on 27 other plant species in 18 families during the surveys conducted in 18 districts of Karnataka from 2015 to 2021. The possible role of S. dorsalis as a vector of tospoviruses in celery needs to be investigated.

Acclimating leaf celery plant (Apium graveolens) via bottom wet culture for increasing its adaptability to tropical riparian wetland ecosystem

Abstract. Lakitan B, Kartika, Susilawati, Wijaya A. 2021. Acclimating leaf celery plant (Apium graveolens) via bottom wet culture to increase its adaptability to the tropical riparian wetland ecosystem. Biodiversitas 22: 320-328. Bottom-wet culture was set up for acclimating leaf celery plant prior to cultivation at shallow water table conditions. The aim of this research was to evaluate adaptability of leaf celery plants to riparian wetland ecosystem. Leaf celery was selected as potential candidate since natural habitat of its wild relatives is marshlands. Shading at 0%, 20%, and 60% was applied to reduced tropical sunlight intensity. Results of this study indicated that soil moisture was significantly increased in plants exposed to 60% shading, but leaf SPAD value was not significantly affected. Leaf celery is a perennial vegetable that can be frequently harvested. Weekly harvesting was rewarded with optimum yield and good quality leaves, i.e. high SPAD value (45.73 to 51.89). Delaying harvest to 3 weeks increased total yield but 52.12% of the harvested leaves were non-marketable. Mother plant of leaf celery produced suckers, but number of suckers only moderately correlated with yield (R2 = 0.56). Plants exposed to 60% shading produced significantly less suckers (9.00) than those exposed to full sunlight (12.46) and 20% shading (12.88) Use of zero intercept linear regression model, with length of leaf midrib (LLM) x leaf wingspan (LWS) as predictor, resulted in a geometrically based and accurate leaf area estimation model (LA = 0.3431(LLM x LWS); R2 = 0.87) for compound leaves of leaf celery plant. In conclusion, the most crucial factor in optimizing quantity and quality of yield was weekly harvesting focusing on marketable-size leaves.

The effect of vertical gardens on temperature and CO2 levels in urban housing

The city's thermal environment plays an important role in achieving comfort and quality of life, especially during the current global pandemic. Meanwhile, reduction in the green areas has been observed to be continuously causing climate change in cities and one of the proposed solutions to this is by developing a greening system for buildings. This paper, therefore, assessed the ability of vertical garden to decrease air and surface temperatures as well as CO2 levels. The research involved field measurements of these parameters both inside and outside the building along with the modification of the vertical garden distance at 0.5 m and 1 m as well as the plant type including red spinach or amaranthus hybridus, mustard or brassica juncea, celery or apium graveolens linn, and cat's whiskers or orthosiphon spicatus. The results showed an average decrease of 0.75°C in air temperature, 16.4ºC in surface temperature, and 58.8 ppm in CO2 levels. Moreover, a maximum reduction of 6ºC was achieved in air temperature with red spinach plants at 0.5 m, 26.3ºC in surface temperature by the Rred spinach plant, and 124 ppm in CO2 levels by celery plants. In conclusion, a closer distance and darker color of the leaves as well as the red spinach species were found to be the main consideration in the application of vertical gardens in urban homes due to their ability to reduce the temperature on the limited land.
 © 2020 Agung Murti Nugroho

Celery (Apium graveolens L.) Performances as Subjected to Different Sources of Protein Hydrolysates.

The vegetable production sector is currently fronting several issues mainly connected to the increasing demand of high quality food produced in accordance with sustainable horticultural technologies. The application of biostimulants, particularly protein hydrolysates (PHs), might be favorable to optimize water and mineral uptake and plant utilization and to increase both production performance and quality feature of vegetable crops. The present study was carried out on celery plants grown in a tunnel to appraise the influence of two PHs, a plant-derived PH (P-PH), obtained from soy extract and an animal PH (A-PH), derived from hydrolyzed animal epithelium (waste from bovine tanneries) on yield, yield components (head height, root collar diameter, and number of stalks), mineral composition, nutritional and functional features, as well as the economic profitability of PHs applications. Fresh weight in A-PH and P-PH treated plants was 8.3% and 38.2% higher, respectively than in untreated control plants. However, no significant difference was found between A-PH treated plants and control plants in terms of fresh weight. Head height significantly increased by 5.5% and 16.3% in A-PH and P-PH treated plants, respectively compared with untreated control (p ≤ 0.05). N content was inferior in PHs treated plants than in untreated control. Conversely, K and Mg content was higher in A-PH and P-PH treated plants as compared to the untreated ones. Furthermore, A-PH and P-PH improved ascorbic acid content by 8.2% and 8.7%, respectively compared with the non-treated control (p ≤ 0.001). Our results confirmed, also, that PHs application is an eco-friendly technique to improve total phenolic content in celery plants. In support of this, our findings revealed that animal or plants PH applications increased total phenolics by 36.9% and 20.8%, respectively compared with untreated plants (p ≤ 0.001).

First Report of Cercospora apiicola Causing Leaf Spots and Stem Lesions on Celery in China.

Celery (Apium graveolens L.) is a vegetable crop cultivated widely in the Mediterranean, Europe and parts of Asia. From March to May in 2014, leaf spots and stem lesions were observed on celery plants in Yanqing (116°03'E, 40°32'N), Beijing and Chengdu (104°06'E, 30°67'N), Sichuan Province. Plants developed 0.3-1.8 cm diameter subcircular leaf spots with brown centers surrounded by pale yellow halos. Spots on leaves were amphigenous. Necrotic areas on stems were subcircular to elongated, pale brown to brown. Plants in five greenhouses were surveyed with 30 to 60% disease incidence. Necrotic tissue from 8 stems and 12 leaves were cut from the margins of lesions and divided into two parts. One part was treated with lactophenol and used for microscopic examination. The other part was surface sterilized with 4% sodium hypochlorite for 2 min, rinsed three times in sterile water, placed onto 2% malt extract agar (MEA), and incubated at 26°C for seven days with natural daylight. Stromata on leaves and stems were not well developed. Four-to-ten conidiophores (15.3-56.5 × 2.8-5.5 μm) formed in fascicles, emerged through stomata or erupted through the cuticle. Conidia (n=50) were 60-135 × 2.5-4.5 μm, solitary, septate, cylindrical to obclavate-cylindrical, hila thickened and darkened. Colonies were white to smoke-gray, and aerial mycelia were sparse to moderate. Morphological characteristics of the pathogen were similar to Cercospora apiicola (Groenewald et al. 2006; Groenewald et al. 2013). The gDNA of 20 isolates was extracted from mycelium using the Plant Genomic DNA Kit (Tiangen, China). The internal transcribed spacers (ITS), actin (ACT), translation elongation factor 1-α (TEF1) and histone H3 (HIS3) regions were amplified with primer pairs ITS1/ITS4 (Groenewald et al. 2013), ACT-512F/ACT-783R (Carbone and Kohn 1999), EF1-728F/EF1-986R (Carbone and Kohn 1999), CYLH3F/CYLH3R (Crous et al. 2006). Phylogenetic analysis of multiple genes (Bakhshi et al. 2018) was conducted with the neighbor-joining method using MEGA7. The sequences of our isolate (QC14030702) and five published sequences of C. apiicola were clustered into one clade with a 99% confidence level. The sequences of QC14030702 have been deposited in GenBank with accessions KU870468 for ITS, KU870469 for ACT, KU870470 for TEF1, and KU870471 for HIS3. Pathogenicity of the isolates was tested on plants (cv. Jia Yuan Xi Yang Qin). Because the pathogen sporulated poorly on various media, mycelial fragments were sprayed on leaves in a suspension of 1×106 mL-1 in a greenhouse (temperature 26±0.5°C; RH 98%; photoperiod 12 h). Healthy plants were sprayed with sterilized water as controls. Three replicates of every isolate were conducted, and each replicate included 5 celery plants. After 7 days, leaf spots appeared on all inoculated plants, which were similar to those on celery in the field. All control plants remained asymptomatic. Re-isolation of the fungus from infected tissues showed same morphological and cultural characteristics of C. apiicola as the original isolates. C. apiicola has been reported in Greece, Korea, South Korea and Venezuela on celery, but never been reported in China (Farr and Rossman 2020). C. apiicola potential threatens celery production, and this the first report of the disease in China.

Spatial Distribution of Listroderes costirostris and Hypera postica (Curculionidae: Cyclominae, Hyperinae) on a Celery Crop in Mexico's Northwest Region

Celery, Apium graveolens L. (Apiaceae), is an essential salad ingredient in different parts of the world, and production is principally destined for the fresh market. Celery hosts several insect species, including the weevil Listroderes costirostris Schoenher (Coleoptera: Curculionidae). Listroderes costirostris currently is present in the San Quintin Valle, Baja California, Mexico, on commercial celery crops. Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) feeds on several plants of the Fabaceae family and is the principal pest affecting Medicago sativa L. (Fabaceae) alfalfa crops. The purpose of this work was to assess the degree of infestation, spatial distribution, and the association between L. costirostris and H. postica on A. graveolens, in Baja California, Mexico. We established 4 quadrants in 1 ha, and we sampled 100 plants per quadrant in each sample. We collected 22 samplings, counting the larvae and adults of both species. Hypera postica was reported herein for the first time feeding on A. graveolens plants. Between 9.57% and 28.68% of the celery plants served as hosts to both species. Listroderes costirostris had the highest percentage of infested plants. The competitive interaction of these 2 species affected H. postica, which showed lesser abundance. Both species were associated in all the sample quadrants. Listroderes costirostris registered an aggregated distribution pattern, whereas H. postica presented a uniform distribution. The spatial distribution of H. postica is the result of competing for food and space with L. costirostris.

Medium-Term Influence of Organic Fertilization on the Quality and Yield of a Celery Crop

For some years now, part of society has been demanding the implementation of circular economy models and so the use of organic matter as a source of nutrients is once again taking center stage. In this scenario, the aim of this work was to implement an integrated management model for a farm and to study the influence on a celery crop of organic amendments (animal and vegetable) obtained on the farm, as opposed to inorganic fertilization. This influence was evaluated for the yield and the nutritional, organoleptic, and sanitary quality of the resulting crops. The yield and size of the marketable parts of the celery plants were greater with the inorganic treatment; however, the nutritional and sanitary quality was better in the organic treatments, while the chromatic attributes, as well as the total P and Ca, were not affected by the different fertilization treatments applied. It is therefore concluded that the organic management model is environmentally and economically sustainable.

Biocontrol actinomycetes better protects cell membranes in celery (apium graveolens l.) under freezing stress in the presence of fungal pathogen

Beneficial microbes can mitigate biotic or abiotic stress-induced damage to plant cell membranes. Yet, little is known about the effects of actinomycetes on cell membrane permeability in plants under joint biotic and abiotic stresses. Herein, the effects of three biocontrol actinomycetes (Streptomyces pactum Act12, S. globisporus Act7, and S. globisporus subsp. globisporus C28) on cell membrane permeability in the leaves of celery (Apium graveolens L. cv. “Hanyusiji”) were evaluated under fungal pathogen (biotic) and freezing (abiotic) stresses by using electrical conductivity measurements. Our results showed that, under freezing stress, any of three fungal pathogens alone resulted in increased cell membrane permeability. Under the single stress of freezing, medium and high concentrations of C28 respectively reduced cell membrane permeability by 37.0% and 30.6%; Act7 exerted no significant effects, whereas high concentration of Act12 increased cell membrane permeability. Under the dual stresses of fungal pathogen and freezing, these protective effects of Act12, Act7, and C28 did not differ significantly. Nonetheless, these protective effects depended on the type of pathogen infection involved: the largest reduction in cell membrane permeability occurred in the presence of F. oxysporum f. sp. vasinfectum (46.4-69.2%) followed by A. alternate (17.4-51.8%), with F. sambucinum ranked lowest (8.8-35.5%). In conclusion, inoculating an appropriate concentration of actinomycetes can mitigate freezing-induced cell membrane injury in celery plants. Importantly, the actinomycete strains better protected the cell membrane against freezing injury under fungal pathogen stress, but this benefit depends on the adverse effects of pathogens on cell membrane permeability.

PENGARUH PUPUK KASCING DAN NPK MUTIARA 16:16:16 TERHADAP PERTUMBUHAN SERTA HASIL TANAMAN SELEDRI (Apium graviolens L.)

ABSTRACT
 The purpose of this study is to determine the effect of interaction and the main influence of vermicompost and NPK Pearl 16:16:16 on the growth and yield of celery plants. This research was carried out for four months, from August to November 2018. This research used a factorial completely randomized design (RAL) consisting of two factors, where the first factor was vermicompost fertilizer (K) consisting of 4 levels, namely 0, 60, 80, 100 g / polybag and the second factor is NPK Pearl 16:16:16 (N) fertilizer consisting of 4 levels, 0, 0.8, 1.6, 2.4 g/polybag. The observation parameters in this study were plant height (cm), relative growth rate (g), root volume (cm3), number of tillers, number of midribs, wet weight of crop (g), and age of harvest (days). The last observation data were statistically analyzed and continued with a BNJ follow-up test at the 5% level. The results showed that the interaction between vermicompost and NPK Pearl 16:16:16 fertilizer significantly affected plant height from week two to week seven, the relative growth rate in the first week, root volume, number of tillers, number of fronds and wet weight per plant. The best treatment is K3N3 (K3 vermicompost fertilizer = 100 g /polybag and NPK Pearl 16:16:16 N3 = 2,4 g/polybag), but it has no significant effect on the age of harvest and the main effect of vermicompost and NPK Pearl 16:16: 16 significantly affected plant height, relative growth rate, root volume, number of tillers, number of midribs and wet weight of the crop, with the best treatment being K3 (vermicompost fertilizer 100 g/polybag) and N3 (NPK Pearl 16:16:16 2,4 g/polybag).
 Keywords: Celery, Vermicompost, NPK pearl 16:16:16