You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Kidney & Bladder (II)1 Apr 20131161 CD1D-RESTRICTED T CELLS MODULATE SUSCEPTIBILITY TO UROPATHOGENIC E. COLI Vladimir Pavlov, Anthony Schaeffer, and David Klumpp Vladimir PavlovVladimir Pavlov Chicago, IL More articles by this author , Anthony SchaefferAnthony Schaeffer Chicago, IL More articles by this author , and David KlumppDavid Klumpp Chicago, IL More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.796AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Natural killer T cells (NKTs) are variously immunostimulatory and immunosuppressive, depending on the specific tissue and pathogenic insult. NKT cells exist in distinct subsets that vary by function, surface markers, and cytokines. But the role of these cells in UTI is unclear. Here, we characterized bladder NKT cells at rest and in response to UPEC infection in a murine UTI model. METHODS Female C57BL/6 or CD1d-deficient mice were instilled via transurethral catheter with UPEC isolate NU14 to evaluate NKT in response to infection by flow cytometry. To demonstrate the functional significance of NKTs in UTI, bladder colonization was quantified in bladder homogenates of wild type and CD1d-deficient mice. RESULTS NK1.1-positive cells constituted 12% of bladder lamina propria T cells and were distributed among CD4-positive (14%) and CD4-negative T-cell subsets (81%). We found that urothelium-expressed CD1 receptor mediates presence of NKT cells in CD4-positive and CD4-negative subpopulations of bladder T lymphocytes (6% of NK1.1+ T cells fewer than wild type mice). NU14 infection increased expression of T cell activation markers CD69, CD62L, and CD44, whereas CD25 was expressed but unaltered by infection. CD1d-deficent mice had significantly decreased NU14 colonization at 24 and 72 hours post-infection. CONCLUSIONS Bladder NKT cells are activated during UTI. Mice deficient in NKT cells exhibit enhanced clearance of UPEC, suggesting that bladder NKT cells suppress innate immunity. Thus, NKT cells are a novel target for enhancing bacterial clearance and promoting effective bladder immunity. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e474 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Vladimir Pavlov Chicago, IL More articles by this author Anthony Schaeffer Chicago, IL More articles by this author David Klumpp Chicago, IL More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...