Cd-contaminated Rice Research Articles

Screening nitrogen-fixing cyanobacteria that can alleviate cadmium toxicity to rice and reduce cadmium accumulation in brown rice

Among food crops, rice is the most serious food crop contaminated with cadmium (Cd). It is critical to reduce Cd bioavailability in the soil and prevent its accumulation in brown rice. The use of microorganisms to lessen Cd toxicity in rice has been the subject of numerous studies carried out in recent years. The nitrogen-fixing cyanobacterium (NFC) is a potent biofertilizer and heavy metal biosorbent. However, there is a dearth of research on the impact of NFC supplementation on rice's susceptibility to Cd poisoning. This study screened for Cd-tolerant NFCs and assessed how well they might promote growth and lower the amount of Cd in rice. Four NFCs with tolerance to 1 mg L−1 Cd were selected from ten NFCs. The results of seedling experiment showed that SCAU-10 treatment increased plant height and biomass while reducing Cd levels in seedlings by 62.0 %. The pot experiment also demonstrated that SCAU-10 was effective in lowering Cd content in brown rice by 85.3 %. Reducing the bioavailability of soil Cd, shielding rice roots, preventing Cd transfer from below to above ground, generating phytohormones, and enhancing the photochemical electron quantum yield of rice leaves are a few possible action mechanisms of the NFC. It indicates that Trichormus sp. SCAU-10 has a great deal of promise for use as a biofertilizer in the safe management of Cd-contaminated rice fields. This is the first report on using Trichormus sp. biofertilizer for relieving the toxicity of Cd to rice and reducing Cd accumulation in brown rice.

Bioaccessibility and bioavailability assessment of cadmium in rice: In vitro simulators with/without gut microbiota and validation through in vivo mouse and human data

Assessing the bioaccessibility and bioavailability of cadmium (Cd) is crucial for effective evaluation of the exposure risk associated with intake of Cd-contaminated rice. However, limited studies have investigated the influence of gut microbiota on these two significant factors. In this study, we utilized in vitro gastrointestinal simulators, specifically the RIVM-M (with human gut microbial communities) and the RIVM model (without gut microbial communities), to determine the bioaccessibility of Cd in rice. Additionally, we employed the Caco-2 cell model to assess bioavailability. Our findings provide compelling evidence that gut microbiota significantly reduces Cd bioaccessibility and bioavailability (p<0.05). Notably, strong in vivo-in vitro correlations (IVIVC) were observed between the in vitro bioaccessibilities and bioavailabilities, as compared to the results obtained from an in vivo mouse bioassay (R2 = 0.63–0.65 and 0.45–0.70, respectively). Minerals such as copper (Cu) and iron (Fe) in the food matrix were found to be negatively correlated with Cd bioaccessibility in rice. Furthermore, the results obtained from the toxicokinetic (TK) model revealed that the predicted urinary Cd levels in the Chinese population, based on dietary Cd intake adjusted by in vitro bioaccessibility from the RIVM-M model, were consistent with the actual measured levels (p > 0.05). These results indicated that the RIVM-M model represents a potent approach for measuring Cd bioaccessibility and underscore the crucial role of gut microbiota in the digestion and absorption process of Cd. The implementation of these in vitro methods holds promise for reducing uncertainties in dietary exposure assessment.

Can cadmium-contaminated rice be used to produce food additive sodium erythorbate?

Cadmium (Cd) in rice is a significant concern for its quality and safety. Currently, there is a crucial need to develop cost-effective and efficient ways to remove Cd or re-utilize Cd-contaminated rice. The food additive sodium erythorbate is produced via 2-ketogluconic acid (2KGA) fermentation by Pseudomonas plecoglossicida and lactonization using starch-rich raw materials, such as rice. We aimed to determine whether cadmium-contaminated rice can be used to produce sodium erythorbate. To achieve this aim, the migration of cadmium during the production of sodium erythorbate from Cd-contaminated rice was studied. Five rice varieties with different Cd contents from 0.10 to 0.68 mg/kg were used as raw materials. The results indicated the presence of Cd in rice and CaCO3 did not have a notable impact on the fermentation performance of 2KGA. The acidification of 2KGA fermentation broth, the addition of K4Fe(CN)6·3H2O and ZnSO4, and 2KGA purification using cation exchange effectively removed >98% of the Cd in the fermentation broth, but the 2KGA yield remained high at approximately 94%. The sodium erythorbate synthesized from Cd-contaminated rice was of high quality and free from Cd, meeting the requirements of the Chinese National Standard, GB 1886.28–2016. The study provided a safe and effective strategy for comprehensively utilizing Cd-contaminated rice to produce high value-added food additive.

Microplastic co-exposure elevates cadmium accumulation in mouse tissue after rice consumption: Mechanisms and health implications

Rice cadmium (Cd) and microplastics are prevalent contaminants, posing a co-exposure threat to humans by means of dietary intake. To assess whether co-exposure of microplastics affects the bioavailability of rice Cd, mice were exposed to Cd-contaminated rice with microplastic co-exposure. We found that polyethylene (PE), polystyrene (PS), polypropylene (PP), and polyamide (PA) microplastic co-exposure via diet consumption (2 μg g–1) caused 1.17–1.38-fold higher Cd accumulation in tissue of mice fed by Cd-rice. For mice with co-exposure of PE microplastics, the higher rice-Cd bioavailability corresponded to colonization of Lactobacillus reuteri (38.9 % vs 17.5 %) in the gut compared to control mice, which caused higher production of gut metabolites particularly peptides, likely causing a ‘side effect’ of elevating Cd solubility in the intestinal lumen. In addition, abundance of sphingosine 1-phosphate in the gut of mice was reduced under PE microplastic exposure, which may reduce intracellular calcium ions (Ca2+) in enterocytes and form a weaker competition in pumping of intracellular Ca2+ and Cd2+ across the basolateral membrane of enterocytes, leading to higher Cd2+ transport efficiency. The results suggest elevated Cd exposure risk from rice consumption with microplastic co-exposure at environmentally relevant low concentrations.

Integrating Transcriptomics and Proteomics to Characterize the Intestinal Responses to Cadmium Exposure Using a Piglet Model.

Cadmium (Cd) is a heavy metal element with a wide range of hazards and severe biotoxicity. Since Cd can be easily accumulated in the edible parts of plants, the exposure of humans to Cd is mainly through the intake of Cd-contaminated food. However, the intestinal responses to Cd exposure are not completely characterized. Herein, we simulated laboratory and environmental Cd exposure by feeding the piglets with CdCl2-added rice and Cd-contaminated rice (Cdcr) contained diet, as piglets show anatomical and physiological similarities to humans. Subsequent analysis of the metal element concentrations showed that exposure to the two types of Cd significantly increased Cd levels in piglets. After verifying the expression of major Cd transporters by Western blots, multi-omics further expanded the possible transporters of Cd and found Cd exposure causes wide alterations in the metabolism of piglets. Of significance, CdCl2 and Cdcr exhibited different body distribution and metabolic rewiring, and Cdcr had stronger carcinogenic and diabetes-inducing potential. Together, our results indicate that CdCl2 had a significant difference compared with Cdcr, which has important implications for a more intense study of Cd toxicity.

Mitigating cadmium exposure risk in rice with foliar nano-selenium: Investigations through Caco-2 human cell line in-vivo bioavailability assay

The contamination of paddy fields by cadmium and lead is a major issue in China. The consumption of rice grown in heavy metals contaminated areas poses severe health risks to humans, where bioavailability and bioaccessibility remains the critical factor for risk determination. Selenium nanoparticles (Se-NPs) can mitigate the toxicity of heavy metals in plants. However, there exists limited information regarding the role of Se-NPs in dictating cadmium (Cd) toxicity in rice for human consumption. Moreover, the impact of Se-NPs under simultaneous field and laboratory controlled conditions is rarely documented. To address this knowledge gap, a field experiment was conducted followed by laboratory scale bioavailability assays. Foliar application of Se-NPs and selenite (at 5, 10 mg L−1) was performed to assess their efficiency in lowering Cd accumulation, promoting Se biofortification in rice grains, and evaluating Cd exposure risk from contaminated rice. Obtained results indicate that foliar treatments significantly reduced the heavy metal accumulation in rice grains. Specifically, Se-NP 10 mg L−1 demonstrated higher efficiency, reducing Cd and Pb by 56 and 32 % respectively. However, inconsistent trends for bioavailable Cd (0.03 mg kg−1) and bioaccessible (0.04 mg kg−1) were observed while simulated human rice intake. Furthermore, the foliage application of Se-NPs and selenite improved rice quality by elevating Se, Zn, Fe, and protein levels, while lowering phytic acid content in rice grains. In summary, this study suggests the promising potential of foliage spraying of Se-NPs in lowering the health risks associated with consuming Cd-contaminated rice.

Sex-dependent effects of rice cadmium exposure on body weight, gut microflora, and kidney metabolomics based on a mouse model

Consumption of cadmium (Cd) contaminated rice is the main dietary source of Cd exposure and toxicity. To protect humans from Cd toxicity, it is pivotal to fully understand the sex-dependent toxicity of subchronic rice-Cd exposure. However, the sex-dependent effects of subchronic rice-Cd exposure on body weight gain, gut microflora, and kidney metabolomics are still unclear. In this study, a Cd-free and a Cd-contaminated rice (0.005 and 0.74 mg Cd kg−1) were fed to both female and male mice for one month, with changes in body weight gain, Cd accumulation in tissue, bone mineral concentration, expression of intestinal channels involving in Cd and calcium (Ca) absorption, gut microbiota, and kidney metabolites assessed for both genders. Results showed that female mice had normal body weight gain after rice-Cd exposure, while body weight of male mice was decreased from 19.8 to 17.5 g over the one-month consumption of the Cd-contaminated rice (0.74 mg kg−1), suggesting specific toxicity on growth of male mice. Rice-Cd exposure had limited effects on gut microbiota for both genders. However, higher Cd accumulation in liver and femur was observed in male mice than in females, which may be due to higher intestinal expression of Ca channels involving in intestinal Cd absorption in male mice with rice-Cd exposure. Greater risk of osteoporosis was also observed in male mice. In addition, kidney metabolomic profiling showed special disruption of adrenocortical hormone homeostasis for male mice with rice-Cd exposure. Particularly, expression of cortisol in kidneys of male mice was elevated 37.1-fold with rice-Cd exposure, likely resulting in Cushing's syndrome and contributing to growth retardation. This study advances our understanding of the sex-dependent toxicity of rice-Cd exposure, and highlights the priority of protecting males from the adrenocortical hormone disrupting effects of rice-Cd exposure.

Mitigation strategies for excessive cadmium in rice.

Cadmium (Cd)-contaminated rice is a human food safety problem that lacks a clear solution. A large amount of rice having an excessive Cd content is processed yearly, but it cannot be discarded and placed in landfills because it will cause secondary pollution. How do we best cope with this toxic rice? From the perspectives of food safety, food waste prevention, and human hunger eradication, the use of contemporary physical, chemical, and biological techniques to lower the Cd content in postharvest Cd-contaminated rice so that it can be used safely is the best course of action. In this review, the contamination, chemical speciation, and distribution of Cd in rice are analyzed and discussed, as are the methods of Cd removal from rice, including a comparison of the advantages and disadvantages of various techniques. Owing to the limitations of current technology, research and technological development recommendations for removing Cd from rice grain are presented. The chemical and biological methods produce higher Cd-removal rates than physical methods. However, they are limited to small-scale laboratory applications and cannot be applied on a large industrial scale. For the efficient safe removal of Cd from food, mixed fermentation with lactic acid bacteria and yeast has good application prospects. However, limited strains having high Cd-removal rates have been screened. In addition, modern biotechnology has rarely been applied to reduce rice Cd levels. Therefore, applying genetic engineering techniques to rapidly obtain microorganisms with high Cd-removal rates in rice should be the focus of future research.

Effects of Straw Removal Measure on Soil Cd Bioavailability and Rice Cd Accumulation

A field experiment was conducted in a lightly Cd-contaminated rice field in Ningxiang City, Hunan Province, to study the effects of straw removal measures on the soil Cd bioavailability and rice Cd accumulation. The results showed that:① two consecutive seasons of straw removal measures (T1-T4 treatments) effectively increased soil pH by 0.04-0.58 units, reduced soil organic matter by 0.68%-25.87%, and reduced the Cd content of rhizosphere soil by 3.76%-12.78%. ② The proportions of Cd in the acid-extractable fraction and oxidizable fraction decreased, and the proportion of Cd in the residual fraction increased. Furthermore, straw removal measures significantly reduced the bioavailability of Cd in rhizosphere soil, and the Cd contents in TCLP, DTPA, and CaCl2 extracts all significantly decreased compared with those in CK. ③ The straw removal measure could significantly reduce the content of DOC and Cd in soil pore water; and the contents of Cd in soil pore water decreased by 4.54%-40.00% and 2.75%-67.34% under the straw removal measure (T1-T4) for two consecutive seasons, respectively, indicating that DOC was one of the key factors affecting the content of Cd in soil pore water. ④ Two consecutive straw removal measures (T1-T4) reduced the accumulation of Cd in different rice tissues, among which, under the treatment of all straw and root removal (T4), the Cd contents of brown rice in late rice planting in 2020 and early rice planting in 2021 decreased by 18.52% and 39.69%, respectively. Therefore, full or partial removal of straw in Cd-contaminated rice fields is a powerful measure to reduce the risk of exceeding Cd levels in brown rice.

Different composites inhibit Cd accumulation in grains under the rice-oilseed rape rotation mode of karst area: A field study

Ensuring the safe production of food and oil crops in soils with elevated cadmium (Cd) content in karst regions is crucial. We tested a field experiment to examine the long-term remediation effects of compound microorganisms (CM), strong anion exchange adsorbent (SAX), processed oyster shell (POS), and composite humic acids (CHA) on Cd contamination in paddy fields under a rice-oilseed rape rotation system. In comparison to the control group (CK), the application of amendments significantly increased soil pH, cation exchange capacity (CEC), and soil organic matter (SOM) content while markedly decreasing the content of available Cd (ACd). During the rice cultivation season, Cd was predominantly concentrated in the roots. Relative to the control (CK), the Cd content in each organ was significantly reduced. The Cd content in brown rice decreased by 19.18–85.45%. The Cd content in brown rice following different treatments exhibited the order of CM > POS > CHA > SAX, which was lower than the Chinese Food Safety Standard (GB 2762–2017) (0.20 mg/kg). Intriguingly, during the oilseed rape cultivation season, we discovered that oilseed rape possesses potential phytoremediation capabilities, with Cd mainly accumulating in roots and stems. Notably, CHA treatment alone significantly decreased the Cd content in oilseed rape grains to 0.156 mg/kg. CHA treatment also maintained soil pH and SOM content, consistently reduced soil ACd content, and stabilized Cd content in RSF within the rice-oilseed rape rotation system. Importantly, CHA treatment not only enhances crop production but also has a low total cost (1255.230 US$/hm2). Our research demonstrated that CHA provides a consistent and stable remediation effect on Cd-contaminated rice fields within the crop rotation system, as evidenced by the analysis of Cd reduction efficiency, crop yield, soil environmental change, and total cost. These findings offer valuable guidance for sustainable soil utilization and safe production of grain and oil crops in the context of high Cd concentrations in karst mountainous regions.

Dietary intake of household cadmium-contaminated rice caused genome-wide DNA methylation changes on gene/hubs related to metabolic disorders and cancers

Cadmium (Cd) contamination in food has raised broad concerns in food safety and human health. The toxicity of Cd to animals/humans have been widely reported, yet little is known about the health risk of dietary Cd intake at the epigenetic level. Here, we investigated the effect of a household Cd-contaminated rice (Cd-rice) on genome-wide DNA methylation (DNAm) changes in the model mouse. Feeding Cd-rice increased kidney Cd and urinary Cd concentrations compared with the Control rice (low-Cd rice), whereas supplementation of ethylenediamine tetraacetic acid iron sodium salt (NaFeEDTA) in the diet significantly increased urinary Cd and consequently decreased kidney Cd concentrations. Genome-wide DNAm sequencing revealed that dietary Cd-rice exposure caused the differentially methylated sites (DMSs), which were mainly located in the promoter (32.5%), downstream (32.5%), and intron (26.1%) regions of genes. Notably, Cd-rice exposure induced hypermethylation at the promoter sites of genes Caspase-8 and interleukin-1β (Il-1β), and consequently, their expressions were down-regulated. The two genes are critical in apoptosis and inflammation, respectively. In contrast, Cd-rice induced hypomethylation of the gene midline 1 (Mid1), which is vital to neurodevelopment. Furthermore, ‘pathways in cancer’ was significantly enriched as the leading canonical pathway. Supplementation of NaFeEDTA partly alleviated the toxic symptoms and DNAm alternations induced by Cd-rice exposure. These results highlight the broad effects of elevated dietary Cd intake on the level of DNAm, providing epigenetic evidence on the specific endpoints of health risks induced by Cd-rice exposure.

Tracking cadmium pollution from source to receptor: A health-risk focused transfer continuum approach

Cadmium (Cd) exposure poses a substantial risk to human health. Despite this, the multi-stage process through which Cd is released to the environment before being taken up and impacting human receptors has rarely been investigated. Here we utilized an integrated model involving Cd emissions, atmospheric transport, deposition, uptake by rice, receptor ingestion and metabolic processing in quantifying the critical emission sources and human health risks of Cd. Atmospheric Cd emissions in the study area in southeastern China were estimated at 147 kg (2016), with >53 % of emissions from non-ferrous metals (NFM) smelting activities. Atmospheric Cd depositions caused elevated Cd content in soil and rice, accounting for 3–79 % and 50–85 % of, respectively, soil and rice Cd. Cumulative frequency analysis showed that an estimated 1.3 % of predicted urine Cd through the consumption of Cd-contaminated rice and exceeded existing safety standards (1 μg g−1), thus highlighting the risks posed to health from high levels of Cd pollution. Applying stricter industrial emission standards to the NFM sector in particular and effective soil management practices could substantially reduce exposure to Cd pollution. The results contribute to understanding of the Cd transfer process and draw attention to the relative health benefits of interventions aimed at mitigating Cd levels and exposure risks at different stages along the Cd transfer continuum from source to receptor.

Genetic Regulation Mechanism of Cadmium Accumulation and Its Utilization in Rice Breeding.

Cadmium (Cd) is a heavy metal whose pollution in rice fields leads to varying degrees of Cd accumulation in rice. Furthermore, the long-term consumption of Cd-contaminated rice is harmful to human health. Therefore, it is of great theoretical significance and application value to clarify the genetic regulation mechanism of Cd accumulation in rice and cultivate rice varieties with low Cd accumulation for the safe use of Cd-contaminated soils. This review summarizes the effects of Cd on rice growth, yield, and quality; the physiological and molecular mechanisms of Cd absorption in the roots, loading, and transport of Cd in the xylem, the distribution of Cd in nodes, redistribution of Cd in leaves, and accumulation of Cd in the grains; the regulation mechanism of the Cd stress response; and the breeding of rice with low Cd accumulation. Future directions on the genetic regulation of Cd in rice and application are also discussed. This review provides a theoretical basis for studies exploring the genetic regulation of Cd stress in rice. It also offers a basis for formulating effective strategies to reduce the Cd content in rice.

The role of nickel in cadmium accumulation in rice

Rice is one of the world's staple foods. Cadmium (Cd) levels in paddy soil are still increasing, and “Cd-contaminated rice” is a frequent occurrence, posing a serious threat to human health. Therefore, Cd contamination in rice is a key issue in agricultural production that needs to be addressed urgently. The Cd accumulation in rice is closely related to other elements. In this study, the impact of nickel (Ni) on the uptake and accumulation of Cd in rice was revealed, and the mechanism was discussed. Statistical analysis of field data showed that Cd concentration in rice grains decreased exponentially with increasing Ni concentration in paddy soils, which was verified by the hydroponic experiments. Under 5 μmol/L Cd exposure conditions, the addition of Ni (100 μmol/L) reduced the Cd contents in roots, stems, and leaves by 81.6 %, 60.6 %, and 65.9 %, respectively. With the presence of Ni, the amount of iron plaque decreased, and the Cd content in the iron plaque was reduced due to the competition between Ni and Cd for adsorption sites. In addition, the migration of Cd from stems to leaves was reduced. At the same time, the distribution of Cd in the cell was altered, and the concentration of Cd in the root cell walls increased with increasing Ni addition under 5 μmol/L Cd exposure. These findings highlight the critical role of Ni in inhibiting Cd accumulation in rice, and provide important information for understanding the effects of coexisting elements in Cd-contaminated soils on Cd accumulation in crops.

Effect of Cd on Pyrolysis Velocity and Deoxygenation Characteristics of Rice Straw: Analogized with Cd-Impregnated Representative Biomass Components.

The pyrolysis characteristics of cadmium (Cd)-impregnated cellulose, hemicellulose, and lignin were studied to elucidate the pyrolysis velocity and deoxygenation characteristics of Cd-contaminated rice straw. The results show that Cd significantly affects the pyrolysis characteristics of a single biomass component. With a heating rate of 5 °C·min−1 and a Cd loading of 5%, the initial pyrolysis temperature of cellulose and hemicellulose decreases while that of lignin increases. The maximum pyrolysis velocity of cellulose, hemicellulose, and lignin is decreased by 36.6%, 12.4%, and 15.2%, respectively. Cd increases the pyrolysis activation energy of the three components and inhibits their deoxygenation. For the pyrolysis of Cd-contaminated rice straw, both the initial depolymerization temperature and the pyrolysis velocity of hemicellulose is reduced, while the pyrolysis velocity of cellulose is accordingly increased. When Cd loading amplifies to 0.1%, 1%, and 5%, the maximum pyrolysis velocity of hemicellulose is decreased by 7.2%, 10.5%, and 21.3%, while that of cellulose is increased by 8.4%, 62.1%, and 97.3%, respectively. Cd reduces the release of volatiles and gas from rice straw, such as CO2, CO, and oxygen-containing organics, which retains more oxygen and components in the solid fraction. This research suggested that Cd retards the pyrolysis velocity and deoxygenation of rice straw, being therefore beneficial to obtaining more biochar.

Chloride application weakens cadmium immobilization by lime in paddy rice soil

Contamination of agricultural products by cadmium (Cd) is a global health problem, causing chronic abnormalities. The consumption of rice, the most-consumed foods, is an important exposure route of Cd to human body. Chloride (Cl-) is reported to increase Cd uptake by rice; however, the effect on Cd uptake and accumulation by rice in the presence of lime is not clear. Therefore, a pot culture experiment was performed to explore the influence of Cl- on the absorption and accumulation of Cd in rice plants under lime remediation and its possible mechanisms. The results showed that Cl- promoted Cd accumulation in rice grains, mainly because of increased Cd bioavailability in the soil and by impeding the formation of iron plaques on rice roots, which reduced chelating and precipitation of Cd. Moreover, increased overexpression of the main transporters of Cd in rice roots, including OsNramp5, OsNramp1, OsIRTs and OsHMA2, favored the upward translocation of Cd from the root to shoot and increased the transfer factors (TFs) from soil to root, root-stem, leaf to grain, and soil to grain. Therefore, the application of Cl-rich materials to Cd-contaminated rice fields should be avoided during liming of the soil for Cd immobilization.

Recognition method for the health risks of potentially toxic elements in a headwater catchment

The spatial association of potentially toxic elements (PTEs) in soil–crop–groundwater systems is poorly recognised. In this study, the contents of arsenic (As), cadmium (Cd), copper (Cu) and lead (Pb) in paddy soils, rice and groundwater in the Xiancha River catchment were determined. The intrinsic effects of PTEs in soils on their spatial distribution in groundwater and rice were explored. Also, the potential sources and health risks of PTEs in multi-media were investigated. Results showed that the mean contents of As and Cd in soils were 23.86 and 0.26 mg kg−1, respectively. In groundwater, the maximum (average) content of As reached 6.55 (1.84) μg L−1. Moreover, As contents in soils and groundwater showed a sound spatial correlation (q = 0.81), and this is supported by the result of the soil column experiment, indicating homology and the strong vertical migration capacity of As. The non-homologous patterns of Pb, Cu and Cd contaminations in soil–groundwater system suggested that geogenic processes influenced the distribution of these PTEs. Cd presented a poor spatial correlation in soil–rice system, as multiple factors controlled its transfer process. Multivariate statistical analysis results demonstrated that As, Cu and Pb in soils mainly came from agricultural sources, whereas high Cd levels were from mining activities. Additionally, direct consumption of As-contaminated groundwater and Cd-contaminated rice posed significant health risks to local residents. This study, which proposes a risk recognition method used to investigate target PTEs in multi-media, may serve as a valuable reference for further research involving catchments.

Cadmium Speciation Distribution Responses to Soil Properties and Soil Microbes of Plow Layer and Plow Pan Soils in Cadmium-Contaminated Paddy Fields.

Cadmium (Cd) speciation ratio in arable land determines the Cd exposure risk and Cd uptake in crops. However, the driving mechanisms of Cd speciation change on the vertical scale of paddy fields remain poorly understood. In this study, the effects of plow layer and plow pan on Cd speciation distribution were investigated in a long-term Cd-contaminated rice ecosystem. The Cd accumulative effect within rice grain was enhanced with high levels of activated Cd speciation ratios in soils. Activated Cd speciation ratios were higher in plow layer soils, while stabilized Cd speciation ratios were elevated in plow pan soils. Soil physicochemical properties and soil microbes synergistically affected the Cd speciation changes in different ways between the two soil layers. Soil pH and organic elements in plow layer environment directly hindered the transformation of stabilized Cd speciation, while in plow pan environment, soil pH and organic elements indirectly decreased activated Cd speciation ratios and resulted in the accumulation of stabilized Cd speciation via regulating the predominant bacterial taxa. This study will improve our understanding of how soil environments regulate Cd speciation distributions in rice ecosystems and help to seek effective remediation methods of Cd-contaminated paddy fields to reduce the Cd accumulation in rice.

Unveiling Endophytic Bacterial Community Structures of Different Rice Cultivars Grown in a Cadmium-Contaminated Paddy Field.

Endophytic bacteria play potentially important roles in the processes of plant adaptation to the environment. Understanding the composition and dynamics of endophytic bacterial communities under heavy metal (HM) stress can reveal their impacts on host development and stress tolerance. In this study, we investigated root endophytic bacterial communities of different rice cultivars grown in a cadmium (Cd)-contaminated paddy field. These rice cultivars are classified into low (RBQ, 728B, and NX1B) and high (BB and S95B) levels of Cd-accumulating capacity. Our metagenomic analysis targeting 16S rRNA gene sequence data reveals that Proteobacteria, Firmicutes, Actinobacteria, Acidobacteria, Bacteroidetes, and Spirochaetes are predominant root endophytic bacterial phyla of the five rice cultivars that we studied. Principal coordinate analysis shows that the developmental stage of rice governs a larger source of variation in the bacterial communities compared to that of any specific rice cultivar or of the root Cd content. Endophytic bacterial communities during the reproductive stage of rice form a more highly interconnected network and exhibit higher operational taxonomic unit numbers, diversities, and abundance than those during the vegetative stage. Forty-five genera are significantly correlated with Cd content in rice root, notably including positive-correlating Geobacter and Haliangium; and negative-correlating Pseudomonas and Streptacidiphilus. Furthermore, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States analysis shows that functional pathways, such as biosynthesis of siderophore and type II polyketide products, are significantly enhanced during the reproductive stage compared to those during the vegetative stage under Cd stress. The isolated endophytic bacteria from the Cd-contaminated rice roots display high Cd resistance and multiple traits that may promote plant growth, suggesting their potential application in alleviating HM stress on plants. This study describes in detail for the first time the assemblage of the bacterial endophytomes of rice roots under Cd stress and may provide insights into the interactions among endophytes, plants, and HM contamination.

Removal of cadmium from rice grains by acid soaking and quality evaluation of decontaminated rice

Contamination of rice by cadmium (Cd) is threatening a large population in China. In this study, we report that soaking rice grains in a hydrochloric acid (HCl) solution can remove Cd to a desirable extent. The results indicated that the degree of Cd removal was up to 45%∼85% at different soaking times and concentrations of HCl (0.06 M ∼ 0.18 M), which was found to be logarithmically correlated with the reaction time at the optimized liquid–solid ratio of 1:2. Three HCl concentration-dependent mathematical models were established, which revealed various optimal soaking conditions depending on the initial Cd contamination. Four Cd-contaminated rice grain samples with different degrees of contamination were then tested based on the mathematical models, and the final Cd content was reduced to an acceptable extent. Moreover, the physicochemical and food properties of rice flours and rice grains after Cd removal were evaluated to highlight their potential applications.