Bioaccessibility Of Cd Research Articles

Evaluation of single-extraction methods to estimate the oral bioaccessibility of metal(loid)s in soils

Incidental ingestion of polluted soil particles exposes the population to toxic metal(loid)s. To refine the methods of exposure and risk assessment, it is relevant to use bioaccessible concentrations of metal(loid)s determined via in vitro digestion methods. However, some validated methods are complex and costly, involving high technical skills and numerous reagents. The objective of the present study was to evaluate the suitability of four simple chemical extractions to mimic the bioaccessible fraction of As, Cd, and Pb in the gastric (G) and gastrointestinal (GI) phases obtained using the validated UBM (unified bioaccessibility method) test. Acetic acid (0.11 M), citric acid (0.11 M), EDTA (0.16 M), and hydrochloric acid (HCl, 0.65%) were separately tested in 201 soil samples with a wide range of physicochemical parameters and metal(loid)s concentrations. Significant linear relationships were observed with HCl, EDTA, and to a lesser extent with citric acid. For the cheaper HCl method, correlations with the UBM ranged from 0.91 to 0.99 for the G phase and from 0.72 to 0.97 for the GI phase. This test can be used at least as a first-tier screening to assess the oral bioaccessibility of As, Cd, and Pb.

In vitro oral bioaccessibility investigation and human health risk assessment of heavy metals in wheat grains grown near the mines in North China

There is limited research on the effects of gut microbiota on bioaccessibility of heavy metals in wheat grains. In this study, bioaccessibility of heavy metals (Cu, Cd, Pb, and Zn) in wheat was determined to elucidate transfer characteristics in the soil-grain-human systems near two large-scale mining areas in Shandong Province, North China using the physiologically-based extraction test (PBET) in combination with a simulator of human intestinal microbial ecosystems (SHIME). The results showed the bioconcentration factors (BCFs) of Cu, Cd, Pb, and Zn were 0.123–0.327, 0.188–0.478, 0.019–0.099, and 0.262–0.825, respectively. Significant and positive correlations were observed between heavy metals in soils and wheat grains. In the simulated colon phase, bioaccessibility of Cd and Zn significantly decreased to 7.81% and 8.81%, respectively, being 53% and 64% of that in the simulated small intestinal phase. However, bioaccessibility of Pb showed an obvious escalating trend, being 2.4 times higher than that of intestinal incubation. Based on the estimated daily intakes and contribution, the relative high contribution of Cu to the benchmark dose in both phases, Cu metabolism by human gut microbiota should be considered in human health risk assessment regarding wheat consumption.

Soil biological characteristic, nutrient contents and stoichiometry as affected by different types of remediation in a smelter-impacted soil

ABSTRACTIn order to evaluate the ecological risk reductions of copper (Cu) and cadmium (Cd) and the change of nutrient contents and stoichiometry in a smelter-impacted farmland in Guixi, Jiangxi Province, China, with ∼ 800 mg Cu kg−1 soil and 0.8 mg Cd kg−1 soil, an three years in situ experiment was conducted. The field trial consisted of 4 × 5 m plots in a completely randomised block design. Hydroxyapatite was added at 10 g kg−1 soil and Sedum plumbizincicola, Elsholtzia splendens, and Pennisetum sp. were planted. Post-treatment soil and plant samples were collected annually and analysed for Cu and Cd bioaccessibility, soil carbon: nitrogen: phosphorus (C:N:P), and the stoichiometries of soil β-glucosidase (BG), N-acetylglucosaminidase (NAG), and acid phosphatase (AP) activity levels. The results indicated that the hydroxyapatite treatments significantly reduced Cu and Cd bioaccessibility as well as the ratio of C:P and N:P. Moreover, BG, NAG, and AP activity levels all increased relative to those in untreated soil. Plants may also influence soil BG, NAG, and AP activity. This study demonstrated that in situ Cu and Cd stabilisation by hydroxyapatite and phytoextraction is ecologically safe and can alter soil mineral nutrient ecological stoichiometry and enzyme activity.

Use of Dietary Components to Reduce the Bioaccessibility and Bioavailability of Cadmium in Rice.

Reducing Cd bioavailability in the systemic circulation is an alternative strategy to reduce Cd exposure. The influence of 39 dietary components on Cd bioaccessibility in water or rice was determined using an in vitro gastrointestinal model, following which an in vivo bioassay was used to determine the most effective components on Cd bioavailability in rice. The results showed that several components significantly reduced the solubility of Cd (10-98%) in the intestinal phase. Tannic acid, TiO2, zinc gluconate, CaCl2, and proanthocyanidins were the most effective in decreasing Cd bioaccessibility in rice, with reductions of 93-97, 54-61, 32-49, 24-32, and 11-14%, respectively. Upon adding the dietary components, the reduction rates of the Cd-relative bioavailability (Cd-RBA) were 20-58 and 10-31% in the kidneys and the liver, respectively. The results may have important implications for reducing health risks associated with Cd exposure via consumption of rice.

Bioaccessibility and health risk assessment of Pb and Cd in urban dust in Hangzhou, China.

Heavy metals in urban dust can enter the human body through a variety of ways, thus endangering human health. Understanding the bioaccessibility of heavy metals in urban dust is a key to its risk assessment. After the G20 summit in 2016, Hangzhou city has received much attention, including its environmental health risk. The surface dust collected from three different functional areas in Hangzhou were subjected to the in vitro physiologically based extraction test (PBET) to measure the bioaccessibility of Pb and Cd. In terms of spatial variation, the distribution of Pb bioaccessibility was in the order of residential areas > city parks > main roads > the Botanic Garden, while for Cd ordered in city parks > residential areas > main roads > the Botanic Garden. For temporal variation, the bioaccessibility of Pb was higher in autumn and winter, and the bioaccessibility of Cd was higher in spring and autumn. Based on multiple linear statistical analysis, the relationship between the spatial and temporal distribution differences of the bioaccessibility of Pb and Cd in the city and the main components was discussed. Meanwhile, the non-carcinogenic hazard quotients of Pb and the carcinogenic risk of Cd were calculated and showed no harm to human health, except the total Pb in the surface dust with a high non-carcinogenic risk for infants. Urban dust in Hangzhou city has a slight pollution and health risk from Pb. Currently, controlling and reducing the city's Pb emission is the key to maintain Hangzhou city's air quality and matching with its international tourism city.

Health risk assessment and bioaccessibilities of heavy metals for children in soil and dust from urban parks and schools of Jiaozuo, China.

Urban parks and schools sever as the mainly activity areas for children, but risk assessment posed by heavy metals (HMs) from soil and dust in these area has rarely been investigated. In this study, six urban parks and seven schools in Jiaozuo, China, were taken as research objects to understand the contamination level and bioaccessibility of HMs from soil and dust in urban parks and schools. The results indicated that Zn, Cu, Pb, Cd, As, Ni and Co from soil and dust were above the background values, especially Zn and Cd in dust, and As and Cd in soil. Serious Cd pollution was discovered, and respective Cd concentrations in soil and dust were 17.83 and 7.52 times the background value. Additionally, the average concentration and bioaccessibility of Zn, Mn, Pb, Cd, Cr, Ni and Co in dust were both higher than in soil. High concentration and high bioaccessibility of HMs in dust suggested that HMs contamination were serious and universal in Jiaozuo. The concentrations of most HMs were higher in the gastric phase, except for Cu and Cd which remained higher in the intestinal phase. Both in the gastric phase and intestinal phase, Mn, As and Cd in soil and dust both have high bioaccessibility which all exceed 10%. The carcinogenic and non-carcinogenic risks base on the total HMs for children (soil: 7.93, 1.96E-05; dust: 6.44, 3.58E-05) were greater than those for adults (soil: 6.35E-01, 1.32E-05; dust: 5.06E-01, 2.42E-05), and urban parks and schools posed high potential risk for children. Therefore, assessment the risk posed by HMs contamination of soil and dust in urban parks and schools is vital and urgent for children.

Load characteristics, in vitro bioaccessibility and health risk assessment of PM2.5-bounded heavy metals in indoor air of waterpipe and/or cigarette cafes compared to smoking-free cafes

ABSTRACT Samples of PM2.5 particulates were collected from indoor air of 36 cafés, 14 cafés in which only water pipe (WS) was used, eight in which only cigarette was smoked (CC), six in which both waterpipe and cigarette were smoked (WCC), and eight cafés in which no smoking occurred (SFC) in Tehran. After that, the concentration of lead (Pb), cadmium (Cd), nickel(Ni), and chromium (Cr) was examined by atomic absorption spectrophotometer (AAS) (GF 5000, Australia). The results indicated that the ƩMetal concentration (Mean ±SD) in WCC, WC, CC, and SFC cafés were 1118.5±50.42, 663.64±40.79, 425.57±17.55, and 79.02±5.13 ng/m3, respectively. The mean bioaccessibility of Pb, Cr, Ni, and Cd was obtained as 39.7, 31.4, 7.35, and 74.6%, respectively. The results of risk assessment indicated that exposure to heavy metals in the indoor air of smoking cafés of Tehran is considered high.

Environmental availability and oral bioaccessibility of Cd and Pb in anthroposols from dredged river sediments.

Dredging and disposal of sediments onto land sites is a common practice in urban and industrial areas that can present environmental and health risks when the sediments contain metallic elements. The aim of this study was to characterise and study the environmental and toxicological availability of Cd and Pb in anthroposols from dredged river sediments. To do this, 67 surface samples spread over 12 sediment disposal sites in northern France were studied. The results showed substantial heterogeneity for this matrix in terms of physicochemical parameters and contamination degree; however, ascending hierarchical clustering made it possible to classify the samples into eight groups. For each group, the mobile fraction of Cd and Pb was studied using single EDTA extraction, solid-phase distribution was analysed with sequential extractions and toxicological availability was assessed with the oral bioaccessibility test. The results showed that (i) Cd had a higher environmental and toxicological availability than Pb; (ii) this availability depends on the physicochemical characteristics of the matrix; and (iii) it is necessary to take into account the environmental and toxicological availability of contaminants when requalifying these sites in order to propose appropriate management measures. In the first years after sediment disposal, it would appear that the environmental and toxicological availability of Cd and Pb increased (from 52.5 to 71.8% and from 28.9 to 48.9%, respectively, by using EDTA and from 50.2 to 68.5% for Cd with the bioaccessibility test). Further studies would therefore be required to confirm this trend and understand the mechanisms involved.

Maize cultivars relieve health risks of Cd-Polluted Soils: In vitro Cd bioaccessibility and bioavailability

Dietary grain ingestion is the primary route of human exposure to the adverse effects of Cd; therefore, an understanding of the transfer characteristics of Cd in a system involving soil, grain, and humans is crucial for health risk alleviation and pollution control. In this study, Cd bioaccessibility and bioavailability for humans from grains of sweet maize (Zea mays convar. saccharata var. rugosa) cultivars grown on a contaminated field (1.05 mg Cd kg−1 soil) were assessed by combining a simulated in vitro digestion method with a Caco-2 cell model. Results showed that cultivars differed significantly in grain Cd concentration, bioaccessibility, and bioavailability with the corresponding values of 0.07 to 0.20 mg kg−1 DW (dry weight), 4.10 to 6.20%, and 0.01 to 0.04 µg g-1grain, respectively. The estimated daily intake of Cd through sweet maize grain was within the range of 0.04 and 0.25 μg kg−1 body weight, which is lower than the tolerable limit recommended by the Joint FAO/WHO Expert Committee on Food Additives (JEFCA). Conclusively, results from the present study indicate that most grain Cd remains non-bioaccessible and thus might not present adverse health effects in humans. Therefore, sweet maize cultivars could be used to produce healthy food crops in low-to-moderately Cd-contaminated soil.

Study of Cd Content Distribution and Its Bioaccessibility in Edible Tissues of Crab Portunus trituberculatus from the Coastal Area of Shandong, China.

Bioaccessibility study is of great significance to the health risk assessment of trace elements in the seafood. In this work, the most consumed crab (Portunus trituberculatus) in coastal area of Shandong, China was analyzed to study the distribution and the bioaccessibility of cadmium (Cd) in the edible tissues of crab, and the dietary risk from consumption of crab was also evaluated. Results showed that the content of Cd in the whole edible tissues of 109 crab specimens ranged from 0.052 to 8.89 mg/kg ww (wet weight) with mean of 2.26 mg/kg ww. The Cd content in 85% of the crab samples was higher than the national food safety limits (0.5 mg/kg ww) of China. The gender study indicated that there was no significant difference in Cd content in total edible tissues between the males and females (p > 0.05). Cadmium was highly concentrated in the brown meat with mean value of 4.13 mg/kg ww, which was about 5 times higher than that in the white meat (0.75 mg/kg ww). The bioaccessibility of Cd ranged from 48.1 to 71.0% in the white and brown meat. The risk assessment based on the bioaccessibility of Cd revealed that the consumption of the edible crab brown and white meat for adults should be limited in 0.13 kg and 1.56 kg per week respectively.

Effects of Soil Properties and Land Use Types on the Bioaccessibility of Cd, Pb, Cr, and Cu in Dongguan City, China

In order to determine the potential heavy metal contamination in soil across Dongguan City, 124 soil samples from seven land use types were collected, four heavy metals (Cd, Pb, Cr, and Cu) were analyzed. Total Cd, Cr, and Cu contents were significantly higher than the background values for Guangdong Province. Lead bioaccessibility in urban green land was lower than that in industrial and abandoned districts. The bioaccessibility of heavy metals was affected by total metal concentrations, soil properties, and land use types. The results showed that there was a negative correlation between the bioaccessibility of heavy metals (except for Cu) and their total concentrations. Soil pH and organic matter were the main factors affecting the bioaccessibility of Cd, Cr, Pb, and Cu in most land use types. Furthermore, sand, P, and clay also affected Pb, Cr, and Cu bioaccessibility. With the exception of the industrial zone periphery and urban green land, the bioaccessibility of heavy metals was mainly affected by clay.

ASSESSMENT OF A SIMPLE EXTRACTION METHOD TO DETERMINE THE BIOACCESSIBILITY OF POTENTIALLY TOXIC Tl, As, Pb, Cu, Zn AND Cd IN SOILS CONTAMINATED BY MINING-METALLURGICAL WASTE

The potential to determine the gastric bioaccessibility (BA) of Tl, As, Pb(II), Cu(II), Zn(II) and Cd(II) using a simple extraction method was evaluated in selected samples from soils contaminated by mining and metallurgical wastes. The SBRC (Solubility and Bioavailability Research Consortium) test was used as a gastric BA comparative method versus a simple extraction method using HCl solution at pH 1.5. High correlations (r ≥ 0.98, p ˂ 0.01) were obtained between the two extraction methods for Cd(II), Cu(II), Tl and Zn(II), in all samples investigated. Bioaccessible As, however, only showed a high correlation in the metallurgical area (r = 0.99, p ˂ 0.01), but not in the mining area (r = 0.88, p ˂ 0.01). Conversely, Pb(II) showed a better correlation in samples from the mining area (r = 0.99, p ˂ 0.01) than in those of the metallurgical area (r = 0.92, p ˂ 0.01). Three experimental conditions of the extraction procedures ensured the high correlations observed: (1) Maintaining a strict pH control of 1.5 (± 0.1), (2) a stirring time of 1 h (± 1 min), and (3) filtration of the extraction mixture through a membrane of small pore size (0.05 µm). Bioaccessible Tl was detected in all samples, a fact that has not been reported before in these environments, and alerts to potential health risks not previously identified.

Biosafety of cadmium contaminated sediments after treated by indigenous sulfate reducing bacteria: Based on biotic experiments and DGT technique

Sulfate reducing bacteria (SRB) biostabilization has attracted particular attention due to its ability to prevent and control heavy metal pollution. In this study, biotic experiments (immobilisation test of Daphnia (D.) magna, germination experiment of cucumber seeds, and in vitro experiment using gut juices of Sipunculus (S.) nudus) and diffusive gradients in thin films (DGT) technique were performed to investigate the biosafety of cadmium (Cd) contaminated sediments after being treated by indigenous SRB. Results showed that SRB treatment reduced Cd bioaccessibility of sediment to S. nudus, Cd levels in the overlying water and Cd bioavailability to D. magna. However, the treatment increased the biotoxicity of overlying water due to significant reduction in the root length and germination index of cucumber seeds. DGT results confirmed that SRB treatment increased Cd stability in sediment, and reduced its release from the sediment into the overlying water. The biotoxicity of overlying water was not caused by Cd, but possibly by the added culture medium, SRB itself, or its metabolites. More attention is required to assess the safety of SRB treatment when it is used to remediate environmental matrix contaminated by heavy metals.

Heavy metal phytoavailability in a contaminated soil of northeastern Oklahoma as affected by biochar amendment.

High concentrations of heavy metals (HM) in soils have negative impacts on plants, human health, and the environmental quality. The purpose of this study was to evaluate the effects of biochars on the bioaccessibility of Zn, Pb, and Cd in a contaminated soil in the Tar Creek area of NE Oklahoma, as well as on the growth and uptake of these elements by perennial ryegrass (Lolium perenne). Biochars were produced from switchgrass (SGB) and poultry litter (PLB) feedstocks at 700 °C and applied to the soil at 0.0, 0.5, 1.0, 2.0, and 4.0% (w/w), with three replications. Regardless of the feedstock, both soil organic carbon (SOC or OC) and pH increased as the rates of biochars increased, which significantly decreased the HM bioaccessibility (p < 0.01). The Zn and Cd extracted by DTPA were highly correlated (p < 0.0001) with their concentration in ryegrass shoots and roots. Except for some significant positive correlations (p < 0.05), HM concentrations in ryegrass shoots and roots were not correlated with their biomass (p > 0.05). Both bioconcentration factor (BCF) and transfer factor decreased as the rates of biochars applied increased, especially for Pb and Cd (p < 0.01). Our results suggest it is beneficial to use biochars at Tar Creek as a soil amendment to reduce HM bioaccessibility and metal uptake by ryegrass.

Selenium and cadmium in bioaccessible fraction of organic weaning food: Risk assessment and influence of dietary components

BackgroundThe tendency of some sectors of the population to consume organic food has also come to include baby food. Nevertheless, it is necessary to develop studies to support the true nutritional and toxicological value of these products, making special emphasis in several trace elements. To our knowledge, no studies have been conducted on this type of organic food. MethodsWeaning foods with different formulations categorized as organic were analyzed to determine Se and Cd contents as well as its bioaccesibility. The analyses were conducted by electro thermal atomic absorption spectroscopy (ET – AAS) after the treatment of the samples with acid mineralization. Besides, macronutrient analyses (protein, fat and dietary fiber) were also developed. Finally, a novelty statistic approach such as @Risk was used to evaluate contributions to DRI or PTWI of Se and Cd derived for consumption of these weaning foods. ResultsSe content ranged between 2.44–15.4 μg Kg 1. Samples with meat ingredients showed the highest Se contents, while weaning foods consisting of fruits or vegetables presented the lowest concentrations. Se bioccessible concentration ranged between 1.90–4.35 μg Kg−1 with a greater uniformity amongst analyzed samples. Regarding Cd, concentrations of this heavy metal ranged between 1.23 and 3.64 μg Kg−1. Furthermore, Cd bioaccessibility of organic weaning foods ranged between 0.17 and 1.38 μg Kg−1. The solubility of all samples studied was around 20% from the initial Cd concentration. A negative statistical correlation between fat content – Cd bioaccesible (p < 0.05; r = − 0.756) and Cd content – Se bioaccesible (p < 0.05; r = − 0.777) were also found. ConclusionsCd concentrations are considerably lower than those reported in weaning formulas which were not categorized as organic. On the other hand, the analysed organic jars did not represent a significant source of Se. The probabilistic assessment developed, showed that contributions to DRI of Se for infants 1–3 years old by consumption of these weaning foods, are excessively low (15% at best).

Polyaspartate and liquid amino acid fertilizer are appropriate alternatives for promoting the phytoextraction of cadmium and lead in Solanum nigrum L

Traditional metal chelators, such as ethylenediaminetetraacetic acid (EDTA), have been gradually replaced due to their poor biodegradability in soil and high risk of heavy metal leaching into groundwater, which pose high environmental risks to the health of humans and animals. In this study, a liquid amino acid fertilizer (LAAF, waste proteins from hydrolysates of animal carcasses) and polyaspartate (PASP) were used as additives to enhance the phytoextraction of cadmium (Cd) and lead (Pb) from contaminated soil. We conducted pot experiments to investigate the phytoextraction capacity of Solanum nigrum, a Cd accumulator, grown on soil highly contaminated with Cd and Pb in the absence (as controls) or presence of PASP and LAAF. Both PASP and LAAF significantly improved plant growth, Cd accumulation, and total Cd and Pb content in S. nigrum shoots and roots. PASP and LAAF application promoted Cd translocation from roots to shoots in S. nigrum and Cd bio-accessibility in rhizosphere soils, but this was not the case for Pb. Both PASP and LAAF increased Cd and Pb phytoextraction by S. nigrum plants, and Cd phytoextraction was more effective in LAAF-assisted S. nigrum than in PASP-assisted S. nigrum. These findings demonstrate that the low cost and ecofriendly features of recycled waste proteins make them good candidates for chelant-enhanced phytoextraction from heavy metal-contaminated soils.

Fractionation and Bioaccessibility of Manganese, Copper, Zinc, Cadmium, and Lead in Commercial Vegetable and Rice Baby Foods Using Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) with Central Composite Design (CCD)

Herein the bioaccessibility of Mn, Cu, Zn, Cd, and Pb, selected from essential and toxic elements, was determined in commercially sold vegetable purees intended for infant and toddler consumption. Chemical fractionation studies using water, acetone, diethyl ether, chloroform:methanol, and n-hexane were employed to predict the importance of the protein and lipid parts of a matrix to assess the bioaccessibility data. In addition, in-vitro gastrointestinal digestion was performed to determine the bioaccessibility of the elements using a five level, three factor central composite design (CCD) to maximize the elemental solubility. The total elemental concentrations in all of the fractions were determined by inductively coupled plasma – mass spectrometry (ICP-MS). Based on the consumption of one jar of vegetable/rice-based baby food, Zn was 1.3% of the recommended dietary allowance and Mn was 4.2% of the adequate intake level, while Cu was almost 100% of the adequate intake level. Additionally, Pb was always below the detection limit and Cd was sometimes under the detection limit for the percent bioaccessibility. However, in some samples, Cd was as high as 80% of the tolerable weekly intake level depending on the body weight.

Biosorption of cadmium by potential probiotic Pediococcus pentosaceus using in vitro digestion model.

An exponential increase of heavy metal toxicant in the human body is a growing health-related problem. In the present study, Pediococcus pentosaceus FB145 and FB181 strains were isolated from fermented seafood and served as highly Cd-resistant strains. The unchanged structural cells of P. pentosaceus strains after treatment with Cd cations were investigated using a scanning electron microscope energy dispersive X-ray analysis. Furthermore, both P. pentosaceus strains showed strong human gastrointestinal tract resistance properties. The Cd biosorption results fit the pseudo-second-order kinetics with capacities for P. pentosaceus FB145 and FB181 for Cd were 52.8 and 50.3 mg/g, respectively, whereas the maximum biosorption capacities were 52.65 and 50.35 mg/g, respectively. The equilibrium data were well fitted to the Freundlich isotherm model. The binding of Cd to bacterial cells may be caused by the presence of different functional groups such as carboxyl, amide, and phosphate on cell surface, which was confirmed by the Fourier transform infrared-attenuated total reflectance spectra. Moreover, these strains decreased the Cd bioaccessibility by 44.7-46.8% in the in vitro digestion model. These findings indicate that P. pentosaceus FB145 and FB181 are novel potent biosorbent for preventing cadmium toxicity and reducing its absorption into the human body.

Mobility and bioaccessibility of risk elements in the area affected by the long-term opencast coal mining

The potential environmental hazards of risk elements in the area affected by the opencast coal mine and/or coal combustion for plants and animals was assessed by using a suite of laboratory bioaccessibility tests. The chosen sampling area was in the vicinity of the largest coal mine spoil in the Sokolov coal basin (Czech Republic). For an estimation of the oral bioaccessibility of the risk elements in soils, the physiologically based extraction tests were applied. Among the available methods for estimating the pulmonary bioaccessibility of elements, the Gamble’s and Hatch’s tests were chosen. The results showed elevated pseudo-total soil contents of As, Be, Cd, Cu, Pb, V, and Zn. Among these elements, only Cd showed substantial bioaccessibility for plants, as documented by the high Risk Assessment Code, reaching up to 47%, and the highest plant-availability, where the maximum Bioaccumulation Factor in plants reached up to 4.5. The simulated body fluids showed the highest bioaccessibility of Cd, but also substantial bioaccessible pools of As and Be, the elements frequently found at the brown coal mining and processing areas. For better understanding of the risk element bioaccessibility under the specific conditions, the released element pools should be related to the particular soil physicochemical parameters.

Inhalation bioaccessibility of Cd, Cu, Pb and Zn and speciation of Pb in particulate matter fractions from areas with different pollution characteristics in Henan Province, China

Windowsill particulate matter (PM) samples were collected from an area near large lead-smelting facilities in Jiyuan (JP), the urban area of Jiyuan (JU) and the peri-urban area of Mianchi (MC) in Henan, China to investigate the concentration and inhalation bioaccessibility of Cd, Cu, Pb and Zn. The <10 μm portions of the samples were extracted with simulated lung fluid to assess the in vitro inhalation bioaccessibility. Lower concentrations of heavy metals were found in the MC samples than in the JP and the JU samples. The average concentrations of Pb, Cd and Cu in the portions of the same size are in the order of JP samples > JU samples > MC samples. For Pb, Cd and Zn, the maximum inhalation bioaccessibility fraction values are all found in the MC samples, which ranged 3.87–8.79%, while those of the JP and the JU samples are <2%. The Pb speciation analysis with X-ray absorption spectrometry indicate mineral bound Pb, PbS and Pb3(PO4)2 are the predominant Pb species in the JP samples; for the JU sample, organic bound Pb is the predominant Pb species in the 45–125 μm portion, while mineral bound Pb is the predominant Pb species in the 10–45 μm portion; for the MC samples, organic bound Pb is the predominant Pb species, followed by PbS. The results indicate that there is significant accumulation of Pb, Cd, Cu and Zn associated with PM in the area near the lead smelter and in the urban area of Jiyuan, especially Pb and Cd, however, the inhalation bioaccessibility of these metals in samples from the lead smelting impacted area is low, this may be due to the higher proportion of less soluble species of the metals in the samples from this area. However, organic matter bound Pb found in some samples has higher bioaccessibility than other Pb species.