SummaryPot experiments were conducted to investigate the subcellular distribution and chemical forms of cadmium (Cd) in order to understand the accumulation, detoxification, and biological toxicity of Cd in strawberry plants. The results showed that the majority of the Cd in leaf cells (82.3%) was deposited in cell walls, followed by organelles (9.7%), and the solublefraction (8.0%). This distribution pattern remained fairly constant, irrespective of Cd treatment. A similar Cd distribution pattern was found in root cells, although the roots accumulated much higher levels of Cd than the leaves. Sephadex column chromatography showed that the Cd was bound to soluble proteins in the roots. The addition of Cd caused changes in the protein composition of roots, resulting in increased levels of high molecular weight proteins. Sequential solvent extraction showed that the NaCl-extractable fraction contained the highest levels of Cd (89.5%) in leaves as well as in roots. Small amounts of Cd were also present in the extracts prepared using 80% (v/v) ethanol, deionised water, 2% (v/v) acetic acid, or 0.6 M HCl, as well as in the final residues. These results suggest that the preferential accumulation of Cd in roots and in cell walls could provide a mechanism for Cd detoxification.