CCl4-induced Lipid Peroxidation Research Articles

Differences in the carbon tetrachloride-induced damage to components of the smooth and rough endoplasmic reticulum from rat liver

There is a higher activity of ethyl morphine N-demethylase (EM-ase) and cytochrome P-450 (P-450) reductase as well as higher P-450 content in the smooth endoplasmic reticulum (SER) than in the rough endoplasmic reticulum (RER). The extent of the irreversible binding of the14C from14CCl4 to lipids and proteins, as well as the CCl4-induced destruction of P-450 is more intense in SER than in RER while the opposite was found for glucose 6-phosphatase (G6P-ase) destruction. CCl4-induced lipid peroxidation is as intense in SER as is in RER.14C from14CCl4 gets irreversibly bound to ribosomal proteins.

Carbon tetrachloride effect on rat liver and adrenals related to their mixedfunction oxygenase content

In liver: CCl 4 interacts with microsomal cytochrome P-450 (P-450) giving type I spectral changes; 14CCl 4 irreversibly binds to microsomal lipids and less to those of mitochondria; CCl 4-induced lipid peroxidation occurs in microsomes and not in mitochondria; P-450 destruction is intense; “in vitro” CCl 4 increases lysosomal permeability. In adrenals: CCl 4 gives either type I or type II spectral changes by acting on either mitochondria or microsomal P-450 respectively; 14CCl 4 binds irreversibly to a similar extent to either mitochondrial or microsomal lipids; lipid peroxidation occurs in microsomes and less in mitochondria; P-450 destruction occurs in microsomes and not in mitochondria; “in vitro” CCl 4 increases lysosomal permeability.

Effect of drug pretreatment on carbon tetrachloride-induced lipid peroxidation in rat liver microsomal lipids

Treatment of rats with phenobarbital sodium (80 mg/day for 3 days) resulted in a 50% increase in liver microsomal lipid peroxidation in vivo as a response to intragastric administration of carbon tetrachloride. Microsomal lipid peroxidation was measured as conjugated diene absorption ( E 1cm 1% at 235 mμ). By contrast, treatment of rats with SKF-525A 30 minutes before administration of carbon tetrachloride led to a marked diminution (in some cases complete inhibition) of the CCl 4-induced microsomal lipid peroxidation. Marked inhibition by SKF-525A of an in vitro pro-oxidant action of carbon tetrachloride was observed.