Ice Cave Research Articles

Cold-Active Lipase from the Ice Cave Psychrobacter SC65A.3 Strain, a Promising Biocatalyst for Silybin Acylation

Cold-active lipase from the psychrophilic bacterial strain Psychrobacter SC65A.3 isolated from Scarisoara Ice Cave (Romania) was cloned and characterized as an extremophilic biocatalyst for silybin acylation. Structural analyses highlighted conserved motifs confirming a functional lipase and the presence of primary structure elements for catalysis at low temperatures. The recombinant enzyme (PSL2) heterologously expressed in Escherichia coli was purified in one step by affinity chromatography with a yield of 12.08 ± 1.72 µg L−1 of culture and a specific activity of 20.1 ± 3.2 U mg−1 at 25 °C. Functional characterization of PSL2 showed a neutral (7.2) optimal pH and a high thermal stability up to 90 °C. Also, this lipase was stable in the presence of different organic solvents, with 60% residual activity when using 20% DMSO. Kinetic measurements indicated performant catalytic efficiency of PSL2 for different short and long chain fatty acids, with Km in the mM range. The catalytic activity of PSL2 was assessed for silybin acylation with various fatty acids and fatty acid methyl esters, demonstrating a 90% silybin conversion when methyl decanoate ester was used. This result clearly highlights the biocatalytic capability of this new cold-active lipase.

Brief communication: Stalagmite damage by cave ice flow quantitatively assessed by fluid–structure interaction simulations

Abstract. Mechanical damage to stalagmites is commonly observed in mid-latitude caves. Former studies have identified thermoelastic ice expansion as a plausible mechanism for such damage. This study builds on these findings and investigates the role of ice flow along the cave bed as a possible second mechanism in stalagmite damage. Utilizing fluid–structure interaction models based on the finite-element method, forces created by ice flow are simulated for different stalagmite geometries. The resulting effects of such forces on the structural integrity of stalagmites are analyzed and presented. Our results suggest that structural failure of stalagmites caused by ice flow is possible, albeit unlikely.

Comparison of Non-Contact Measurement Technologies Applied on the Underground Glacier—The Choice for Long-Term Monitoring of Ice Changes in Dobšiná Ice Cave

Comparison of Non-Contact Measurement Technologies Applied on the Underground Glacier—The Choice for Long-Term Monitoring of Ice Changes in Dobšiná Ice Cave

Modelling the effect of free convection on permafrost melting rates in frozen rock clefts

Abstract. This research develops a conceptual model of a karst system subject to mountain permafrost. The transient thermal response of a frozen rock cleft after the rise in the atmospheric temperature above the melting temperature of water is investigated using numerical simulations. Free convection in liquid water (i.e. buoyancy-driven flow) is considered. The density increase in water from 0 to 4 °C causes warmer meltwater to flow downwards and colder upwards, resulting in significant enhancement of the heat transferred from the ground surface to the melting front. Free convection increases the melting rate by approximately an order of magnitude compared to a model based on thermal conduction in stagnant water. The model outcomes are compared qualitatively with field data from the Monlesi ice cave (Switzerland) and confirm the agreement between real-world observations and the proposed model when free convection is considered.

Biodiversity of a temperate karst landscape-ice cave collapse doline supports high α-diversity of the soil mesofauna.

Caves represent a specific environment with a special microclimate and fauna adapted to it. However, we have still a restricted knowledge on soil fauna communities in an environments with a marked microclimate gradient at the interface between cave and surface habitats. In the present study, we investigated the community patterns of dominant soil microarthropod group, oribatid mites, and their relationship to environmental factors across the microclimate gradient along a transect with seven study sites from cold and wet cave entrance zone to warm and drier deciduous forest. The cold and wet sites showed low oribatid abundances and a high spatial clustering of individuals in comparison to the warm sites. The oribatid mites showed significantly higher abundance and species richness at warm forest sites. Indicator species analysis indicated specific cold-tolerant species that dominated at cold sites of the gradient, but which were absent at the warmest sites. Variance analysis clarified that community composition at sites were significantly driven by environmental factors: temperature, soil moisture, pH and C/N ratio. The study underlines the importance of ice cave entrances as sources of high α-diversity of soil mesofauna and thus the conservation priority of these valuable habitats.

Environmental and Human Impact on Ice Caves: The Example of the Wielka Śnieżna Cave in the Tatra Mountains (Poland)

Ice caves are characterised by specific microclimate, defined by the external climate as well as cave morphology (hence the location). They are unique components of Earth’s heritage. The ice formed there holds important paleoclimatic information and can be used to assess the global warming effect on the cryosphere in non-glaciated areas. We present an example from Wielka Śnieżna, the deepest cave system in the Polish Tatra Mountains, located in Tatra National Park (Tatrzański Park Narodowy). There have been no recent studies completed on the topic in this location. We provide the first contemporary results of temperature measurements by analysing microclimatic patterns and their response to global warming. There is ongoing ablation of the Ice Fall located in one of the lower entrances (Śnieżna) showing that the ice level has decreased by around 2 m in the last 10 years. The cave is under a minor human impact as it can be accessed only by professional cavers. However, some practices impact the balance of this fragile environment. By comparing our results with the outside temperature records, we aim to understand how dependent the cave microclimate is on the external fluctuations and changes. The ice in the Śnieżna is expected to be gone in the current century.

Radiological monitoring and assessment of the radiation situation in the Kungur Ice Cave

This article presents the results of comprehensive radiological studies conducted in 2021 in order to assess the radiation situation in the Kungur Ice Cave (hereinafter referred to as the KIC). Since the cave is a fairly popular sightseeing object, therefore, the safety of both tourists and workers is a key task for scientific study. The radiation safety assessment was carried out taking into account the parameters of the radiation (gamma) background, measurements of radon and thoron concentrations, microclimatic indicators (air temperature, humidity), airflow rate, illumination and calculation of the indicator of a special assessment of working conditions (SAWC). Such comprehensive and detailed studies were conducted in the KIC for the first time, allowing some patterns to be identified and confirmed. For the indicators of gamma-activity and radon concentration, there is a clear dependence on the season of the year, which is directly related to microclimatic indicators, as well as to compliance with the ventilation regulations that have been established in the cave. The applied ventilation modes provide safe values of the radiation background and toron. In the summer months, the average radon content in the cave exceeds the maximum permissible concentrations by a factor of 7, which required calculation of the time of work in the cave. For the first time, special assessment of working conditions has been calculated for Kungur Ice Cave, but only for the winter period. The SAWC is defined for a working group consisting of engineers, workers and guides, taking into account such parameters as temperature, illumination, humidity, severity and intensity of labor and ionizing radiation (radiation background). According to preliminary data, the overall assessment of labor based on all parameters falls under the “harmful” class of conditions.

Molecular Screening for Cyanobacteria and Their Cyanotoxin Potential in Diverse Habitats.

Cyanobacteria are adaptable and dominant organisms that exist in many harsh and extreme environments due to their great ecological tolerance. They produce various secondary metabolites, including cyanotoxins. While cyanobacteria are well studied in surface waters and some aerial habitats, numerous other habitats and niches remain underexplored. We collected 61 samples of: (i) biofilms from springs, (ii) aerial microbial mats from buildings and subaerial mats from caves, and (iii) water from borehole wells, caves, alkaline, saline, sulphidic, thermal, and iron springs, rivers, seas, and melted cave ice from five countries (Croatia, Georgia, Italy, Serbia, and Slovenia). We used (q)PCR to detect cyanobacteria (phycocyanin intergenic spacer-PC-IGS and cyanobacteria-specific 16S rRNA gene) and cyanotoxin genes (microcystins-mcyE, saxitoxins-sxtA, cylindrospermopsins-cyrJ), as well as amplicon sequencing and morphological observations for taxonomic identification. Cyanobacteria were detected in samples from caves, a saline spring, and an alkaline spring. While mcyE or sxtA genes were not observed in any sample, cyrJ results showed the presence of a potential cylindrospermopsin producer in a biofilm from a sulphidic spring in Slovenia. This study contributes to our understanding of cyanobacteria occurrence in diverse habitats, including rare and extreme ones, and provides relevant methodological considerations for future research in such environments.

Exploring Ice Cave Biodiversity in Northeastern Italy

The ice stored in caves is a widespread yet neglected cryospheric component. The cold-adapted biodiversity of ice caves has received very little attention from research, despite the potential abundance of endemic troglobiotic and cryophilic species and their consequent sensitivity to the changing climate. In this study, we investigated the invertebrate diversity of two ice caves in Northeastern Italy (Bus delle Taccole and Caverna del Sieson, Veneto Region). During 2022 and 2023, we sampled, using pitfall traps, the invertebrates dwelling at different locations in each cave: the shaft base, an intermediate hall, and the cave bottom. At each cave location, we also collected ice samples, on which we measured the stable isotopes of oxygen and hydrogen (δ18O, δ2H), and monitored the air temperature with data-loggers. The two caves had different invertebrate communities, both dominated by a combination of troglobiotic and cryophilic taxa. Despite a low taxonomic richness, which was higher at Taccole (15 taxa) than at Sieson (11 taxa), both caves hosted rare/endemic species, four of which are not described yet. At each cave, the ice water isotopic signatures differed among cave locations, suggesting the ice had formed under different climatic conditions, and/or resulted from different frequencies of thawing/freezing events. The occurrence of summer melt at both caves suggests that these unique ecosystems will quickly disappear, along with their specialized and unique biodiversity.

Characteristics of methane and carbon dioxide in ice caves at a high-mountain glacier of China

The exploration of the spatiotemporal distribution of greenhouse gas (GHG) exchange in the cryosphere (including ice sheet, glaciers, and permafrost) is important for understanding its future feedback to the atmosphere. Mountain glaciers and ice sheets may be potential sources of GHG emissions, but the magnitude and distribution of GHG emissions from glaciers and ice sheets remain unclear because observation data are lacking. In this study, in situ CH4 and CO2 and the mixing ratios of their carbon isotope signatures in the air inside an ice cave were measured, and CH4 and CO2 exchange in the meltwater of Laohugou glacier No. 12, a high-mountain glacier in an arid region of western China, was also analyzed and compared with the exchange in downstream rivers and a reservoir. The results indicated elevated CH4 mixing ratios (up to 5.7 ppm) and depleted CO2 (down to 168 ppm) in the ice cave, compared to ambient levels during field observations. The CH4 and CO2 fluxes in surface meltwater of the glacier were extremely low compared with their fluxes in rivers from the Tibetan Plateau (TP). CH4 and CO2 mixing ratios in the air inside the ice cave were mainly controlled by local meteorological conditions (air temperature, wind speed and direction) and meltwater runoff. The carbon isotopic compositions of CH4 and CO2 in the ice cave and terminus meltwater indicated δ13C-CH4 depletion compared to ambient air, suggesting an acetate fermentation pathway. The abundances of key genes for methanogenic archaea/genes encoding methyl coenzyme M reductase further indicated the production of CH4 by methanogenic archaea from the subglacial meltwater of high-mountain glaciers. The discovery of CH4 emissions from even small high-mountain glaciers indicates a more prevalent characteristic of glaciers to produce and release CH4 from the subglacial environment than previously believed. Nevertheless, further research is required to understand the relationship between this phenomenon and glacial dynamics in the third pole.

First insights into the age of the giant ice deposits in the Eisriesenwelt cave (Austria)

Frozen water is the most widespread type of ice present in ice caves and forms ice stalagmites and stalactites as well as floor ice, which is often several meters thick. Organic macroremains are commonly rare in this type of cave ice, which makes it difficult to establish a chronology and severely limits the use of such ice deposits as paleoenvironmental archives. Here, the chronology of such ice deposits in the inner part of the glaciated Eisriesenwelt, one of the world’s largest ice caves located in the European Alps of Austria, is determined by a combination of radiocarbon and 230Th dating of cryogenic calcite. The data suggest that this cave ice has formed over the last three millennia, with a marked increase in the average accumulation rate during the thirteenth century, coinciding with the onset of the Little Ice Age in the Alps. Data from a second site closer to the entrance suggests that large parts of this tourist cave were likely ice-free during the Medieval Warm Period and that a substantial part of the ice is probably a relic of the Little Ice Age. The current warming has already penetrated deeper into the cave than during the Medieval Warm Period, although air exchange during the warm season is restricted by a door at the cave entrance.

Extremophiles and their expanding biotechnological applications.

Microbial life is not restricted to any particular setting. Over the past several decades, it has been evident that microbial populations can exist in a wide range of environments, including those with extremes in temperature, pressure, salinity, and pH. Bacteria and Archaea are the two most reported types of microbes that can sustain in extreme environments, such as hot springs, ice caves, acid drainage, and salt marshes. Some can even grow in toxic waste, organic solvents, and heavy metals. These microbes are called extremophiles. There exist certain microorganisms that are found capable of thriving in two or more extreme physiological conditions simultaneously, and are regarded as polyextremophiles. Extremophiles possess several physiological and molecular adaptations including production of extremolytes, ice nucleating proteins, pigments, extremozymes and exopolysaccharides. These metabolites are used in many biotechnological industries for making biofuels, developing new medicines, food additives, cryoprotective agents etc. Further, the study of extremophiles holds great significance in astrobiology. The current review summarizes the diversity of microorganisms inhabiting challenging environments and the biotechnological and therapeutic applications of the active metabolites obtained as a response to stress conditions. Bioprospection of extremophiles provides a progressive direction with significant enhancement in economy. Moreover, the introduction to omics approach including whole genome sequencing, single cell genomics, proteomics, metagenomics etc., has made it possible to find many unique microbial communities that could be otherwise difficult to cultivate using traditional methods. These findings might be capable enough to state that discovery of extremophiles can bring evolution to biotechnology.

Calcium carbonate precipitating extremophilic bacteria in an Alpine ice cave

Extensive research has provided a wealth of data on prokaryotes in caves and their role in biogeochemical cycles. Ice caves in carbonate rocks, however, remain enigmatic environments with limited knowledge of their microbial taxonomic composition. In this study, bacterial and archaeal communities of the Obstans Ice Cave (Carnic Alps, Southern Austria) were analyzed by next-generation amplicon sequencing and by cultivation of bacterial strains at 10 °C and studying their metabolism. The most abundant bacterial taxa were uncultured Burkholderiaceae and Brevundimonas spp. in the drip water, Flavobacterium, Alkanindiges and Polaromonas spp. in the ice, Pseudonocardia, Blastocatella spp., uncultured Pyrinomonadaceae and Sphingomonadaceae in carbonate precipitates, and uncultured Gemmatimonadaceae and Longimicrobiaceae in clastic cave sediments. These taxa are psychrotolerant/psychrophilic and chemoorganotrophic bacteria. On a medium with Mg2+/Ca2+ = 1 at 21 °C and 10 °C, 65% and 35% of the cultivated strains precipitated carbonates, respectively. The first ~ 200 µm-size crystals appeared 2 and 6 weeks after the start of the cultivation experiments at 21 °C and 10 °C, respectively. The crystal structure of these microbially induced carbonate precipitates and their Mg-content are strongly influenced by the Mg2+/Ca2+ ratio of the culture medium. These results suggest that the high diversity of prokaryotic communities detected in cryogenic subsurface environments actively contributes to carbonate precipitation, despite living at the physical limit of the presence of liquid water.

Jaskinia Lodowa w Ciemniaku (Ice Cave in Ciemniak), Western Tatra, Poland - over a century-long investigations of climate warming-caused degradation of subterranean ice mass

Jaskinia Lodowa w Ciemniaku (Ice Cave in Ciemniak), in the Tatra Mountains (Tatry), is believed to host the largest subterranean ice mass in Poland. It has been known for over a century, however, the onset of its scientific investigations dates back to 1922, when Tadeusz and Stefan Zwoliński mapped it. Since then, it has become one of the best-known caves in Poland. It was described in over a hundred scientific and popular science papers. They include findings of international importance, e.g. works related to radioisotopes, ice-mass balance and age. However, some of the questions asked a century ago are still partly unanswered. One may wonder if they will be delivered before climate warming causes the largest ‘cave glacier’ in Poland to disappear.

What is the primary culprit that causes the "Doomsday Glacier" in Antarctica?

Antarctica, situated in the southernmost part of the earth is surrounded by the Southern Ocean. It has an extremely important role in tuning global climate, both because of its geographic location, and its giant ice mass, that comprises ~90% of the world's ice. In the past decades, the Thwaites Glacier in the Amundsen Sea of West Antarctica is undergoing the fastest recession in the region. The ice loss in Thwaites Glacier is currently responsible for roughly four percent of the global sea-level rise, which has been attributed to climate change and ocean warming. Due to the continuous collapsing and melting of Thwaites Glacier and the severe threat to humans, scientists gave it a terrifying name "Doomsday Glacier". With increasingly geological and geophysical studies conducted in West Antarctica, geothermal heat flux has been discovered to play a vital role in icesheet retreating. The rapidly retreating Thwaites and Pope glaciers are underlain by areas of largely elevated geothermal heat flow, which relates to the tectonic and magmatic history of the West Antarctic Rift System in this region, suggesting that this area is coupled to the dynamics of the underlying lithosphere. The collapsing and melting of the West Antarctic glaciers are without doubt a realistic and complex issue. From the current geological surveys, the heat flux of the crust of West Antarctica appears to be accelerating, coupled with frequent earthquakes and volcanoes. Geothermal features such as hot water lakes, thermal rivers, and giant ice caves beneath the glaciers have been continuously discovered. Therefore, it is reasonable to speculate that geothermal effects play a significant role in modifying the vast ice masses, causing glacier sliding, cracking, collapsing, and ultimately melting, and create conditions for a warming climate to melt West Antarctic glaciers. Many studies also suggest that warm ocean water is intruding beneath the glaciers across the grounding line, leading to melting of glacier bottom, which has been generally considered to be associated with human-emitted greenhouse gases, but it is thought here not to be a primary factor for glacier melting, but most likely a secondary factor. It is believed that primary and secondary factors, Milankovitch orbits, black body radiation, solar activities, human activities, and other factors are all interconnected to form a feedback loop between the glacier base and the ocean, or even a positive feedback loop, which further accelerates the collapsing and melting of the west Antarctic glaciers

Comparative studies on radon seasonal variations in various undeground environments: Cases of abandoned Beshtaugorskiy uranium mine and Kungur Ice Cave

It is well known that one of the most important risk factors in underground environment is the harmful effects of radon. The reasons for strong seasonal fluctuations in radon content in underground environments remain not fully understood. The purpose of this article is to improve existing ideas about this phenomenon. The article presents the results of a study of radon transport in two different underground spaces – the Beshtaugorskiy uranium mine (North Caucasus) and the Kungur Ice Cave (Middle Ural). We have used the direct measurements of the equilibrium equivalent concentration (EEC) of radon progeny in air, as well as the air flow velocity. A very wide range and strong seasonal variations in the radon levels have been recorded in both cases. The EEC has a range of 11–6653 by Bq m−3 and 10–89,020 Bq m−3 in the Kungur cave and the Beshtaugorskiy mine, respectively. It has been established that seasonal fluctuations in radon levels both in the mine and in the cave are caused by the same process – convective air circulation in the underground space due to the temperature difference between the mountain massif and the atmosphere (so called chimney effect). Overall, these results indicate that due to convective air circulation, underground spaces are periodically intensively ventilated with atmospheric air, and then, on the contrary, they are filled with radon-enriched air that seeps into caves or adits from rocks and ores. In both cases, the EEC of radon progeny exceeds the permissible level for the population and workers. The results of this study highlight the need for the development of measures to limit the presence of people in the surveyed underground spaces.

Anthropogenic Influence on the Microclimate and Glaciation of the Kungur Ice Cave during the Period of Maximum Tourist Load

Anthropogenic Influence on the Microclimate and Glaciation of the Kungur Ice Cave during the Period of Maximum Tourist Load

Cross-Polarized SfM Photogrammetry for the Spatial Reconstruction of Challenging Surfaces, the Case Study of Dobšiná Ice Cave (Slovakia)

Geodetic methods are integral to mapping surface and subsurface objects and phenomena. Modern geodetic technologies such as laser scanning and digital photogrammetry have also become a standard part of the mapping and documentation of cave spaces. In some cases, these technologies cannot accurately capture the measured surface and thus provide reliable data. One such example is the ice with specific surface characteristics in caves with ice deposits. One of the world’s most studied ice caves is the Dobšiná Ice Cave (Slovakia), which has undergone significant changes in the ice-filling area and volume in recent years. To monitor and analyze all these changes properly, we need to know the surface and volume of this ice mass and monitor it regularly. Where modern geodetic methods such as terrestrial laser scanning (TLS) or digital photogrammetry may fail due to the ice’s physical properties, we propose using cross-polarized Structure-from-Motion (SfM) photogrammetry. As a case study, this method was used in a 28 m long ice tunnel in this cave. Two polarizing filters (on the flash as a light source and on the camera lens) were used in 90° rotation to each other to achieve the cross-polarization effect and remove surface reflections. This removed the surface reflections, giving us a compact and accurate point cloud of the entire tunnel. The dense cloud from cross-polarized (CP) photogrammetry is denser and more compact and does not contain as many outliers and noise points when compared to non-cross-polarized (non-CP) photogrammetry. The TLS point cloud covers the entire surface of the tunnel without significant holes; however, the penetration of the beam through the ice makes such a cloud unusable. Only the cloud from CP photogrammetry covers the entire surface of the tunnel densely enough without additional noise. This methodology can then be used in other parts of the cave or other geomorphological applications to suppress reflections so high-quality results for further processing and analysis can be obtained.

Mode of formation of cryogenic cave carbonates: Experimental evidence from an Alpine ice cave

Coarsely crystalline carbonates that formed cryogenically in caves (CCCcoarse for short) are important paleoenvironmental archives that provide evidence of former perennial ice deposits in currently ice-free caves and allow the timing of the past freezing conditions close to 0 °C in the subsurface to be determined. Despite their significance, the mode of formation of these deposits has never been observed in statu nascendi.We conducted a series of freezing experiments in an Alpine ice cave to test the conceptual model of CCCcoarse formation in freezing pools of water in perennial ice deposits. Our results document a number of features associated with the freezing of such confined water bodies, including ice bulging, cracking and episodic expulsion of water. These observations help explain the degassing of carbon dioxide required to maintain carbonate precipitation in these semi-closed pockets of slowly freezing water. Our experiments yielded a range of morphological types of cryogenic calcite crystals and aggregates comparable to those reported from CCCcoarse from currently ice-free caves elsewhere. The experimentally grown crystals also exhibit the same depleted O isotope composition characteristic of natural carbonates interpreted as being formed subaqueously in a semi-closed pools subject to freezing. The main difference to naturally formed CCCcoarse is their smaller crystal size, reflecting the comparably short freezing time of our experiments of less than two weeks.This study confirms the conceptual model explaining the formation of CCCcoarse in localized pools in ice and calls for a revision of the currently existing classification scheme for cryogenic cave carbonates.

Multiparametric Monitoring System of Mt. Melbourne Volcano (Victoria Land, Antarctica).

Volcano monitoring is the key approach in mitigating the risks associated with volcanic phenomena. Although Antarctic volcanoes are characterized by remoteness, the 2010 Eyjafjallajökull eruption and the 2022 Hunga eruption have reminded us that even the farthest and/or least-known volcanoes can pose significant hazards to large and distant communities. Hence, it is important to also develop monitoring systems in the Antarctic volcanoes, which involves installing and maintaining multiparametric instrument networks. These tasks are particularly challenging in polar regions as the instruments have to face the most extreme climate on the Earth, characterized by very low temperatures and strong winds. In this work, we describe the multiparametric monitoring system recently deployed on the Melbourne volcano (Victoria Land, Antarctica), consisting of seismic, geochemical and thermal sensors together with powering, transmission and acquisition systems. Particular strategies have been applied to make the monitoring stations efficient despite the extreme weather conditions. Fumarolic ice caves, located on the summit area of the Melbourne volcano, were chosen as installation sites as they are protected places where no storm can damage the instruments and temperatures are close to 0 °C all year round. In addition, the choice of instruments and their operating mode has also been driven by the necessity to reduce energy consumption. Indeed, one of the most complicated tasks in Antarctica is powering a remote instrument year-round. The technological solutions found to implement the monitoring system of the Melbourne volcano and described in this work can help create volcano monitoring infrastructures in other polar environments.