Cause Of Numerous Diseases Research Articles

Sperm DNA Fragmentation: Origin and Impact on Human Reproduction

Sperm DNA can be damaged due to a multitude of different noxae, which include disease, and occupational and environmental factors. Depending on the magnitude of the damage, such lesions may be repaired by the oocyte or the embryo. If this is not possible, a permanent damage can be manifested leading to mutations of the male genome. In cases where the oocyte or the embryo does not counter these damages to the male genome in terms of repair or an early abortion, sperm DNA damage and fragmentation can be a cause of numerous diseases including childhood cancer.

Tabagismo em idosos

O tabagismo representa um importante acelerador do processo de envelhecimento, comprometendo não apenas a expectativa, mas a qualidade de vida também. Fumantes com idade superior a 50 anos apresentam maior dependência da nicotina, fumam há mais tempo e um maior número de cigarros, tendo mais problemas de saúde relacionados ao tabagismo, e dificuldade maior em parar de fumar. As principais causas de morte por tabagismo são as doenças cardiovasculares, a doença pulmonar obstrutiva crônica e o câncer de pulmão. Atualmente, há uma série de estratégias farmacológicas e não-farmacológicas para o auxílio do abandono do tabagismo, que também podem ser uma alternativa para os idosos. O objetivo desta revisão é relatar dados do tabagismo no Brasil e no mundo, as patologias envolvidas e os efeitos tóxicos dos componentes do cigarro, bem como sugerir estratégias de tratamento, principalmente para os idosos. A revisão da literatura foi feita nas bases: Pubmed, Scielo, Portal Capes, Ministério da Saúde, Instituto Nacional do Câncer, IBGE e Organização Mundial da Saúde. Concluiu-se, através da literatura consultada, que o tabagismo representa uma fonte de risco e a causa principal de inúmeras doenças. Porém, o desejo de parar de fumar é maior entre aqueles que reconhecem ser esse hábito prejudicial à saúde. Apesar de os benefícios, com a interrupção do hábito de fumar, serem maiores entre os mais jovens, o abandono do tabagismo, em qualquer idade, reduz o risco de morte e melhora a condição geral de saúde.

How a routine checking of Escherichia coli in retailed food of animal origin can protect consumers against exposition to Campylobacter spp. and Listeria monocytogenes?

According to the literature that has been published over the last two decades Campylobacter spp i Listeria monocitogens can be identified as causes of numerous diseases derived by consuming food of animal origin. The purpose of this paper was to find out how established national microbiological criteria of the Republic of Serbia on food safety in retailed food of animal origin could contribute to consumer's protection against exposition to foodborne pathogens such as Campylobacter spp. and Listeria monocytogenes. During a routine microbiological safety control of randomly selected 60 samples of fresh poultry meat, 30 samples of other fresh meat readymade for grilling, 30 samples of sausage products, 37 samples of heat-treated meat, 39 samples of toppings for fast food of animal origin and 31 samples of dairy products a national food safety criteria (Escherichia coli, aerobic plate count, Salmonella spp., coagulasa positive Staphylococcus, Proteus spp., sulphito-reducting Clostridia) were applied and, as well as, testing to Campylobacter spp. and Listeria monocitogens. In determination of Campylobacter spp. and Listeria monocytogenes, food quality control methods of the Food and Agriculture Organization (FAO) were applied, while in determination of the other above motioned bacteria, national provisions on microbiological methods were applied who are adjusted to the FAO ones. Related to the national criteria on microbiological food safety, 88 (38.8%) samples, out of the total 227 tested, were rejected. When to these results, the results of laboratory tests on Listeria monocytogens were added, a terminal number of rejected samples were not changed. When to these results, the results of Campylobacter spp. testing were added, 91 (40.1%) out of the 227 samples were unsatisfied. Results of logistic regression model with occurrence of Escherichia coli as dependent variable indicated that Escherichia coli was 4.5 times likely to occur among samples with Campylobacter spp. than among samples without Campylobacter spp. (OR = 4.515, 95% CI: 1.019-20.002). Sensitivity of the fitted model (Hosmer-Lemeshowp = 0.268) was 76.8% and its specificity was 75.0%. At the same time Escherichia coli was confound in all (100%) food samples that were contaminated by Listeria monocytogenes. Statistical analysis indicated that Escherichia coli was completely sensitive to identify all samples contaminated with Listeria monocytogenas and highly sensitive to identify samples contaminated with Campylobacter spp. Nevertheless, 3 (1.3%) of the tested samples were not covered with Escherichia coli.

A robust penalized method for the analysis of noisy DNA copy number data

BackgroundDeletions and amplifications of the human genomic DNA copy number are the causes of numerous diseases, such as, various forms of cancer. Therefore, the detection of DNA copy number variations (CNV) is important in understanding the genetic basis of many diseases. Various techniques and platforms have been developed for genome-wide analysis of DNA copy number, such as, array-based comparative genomic hybridization (aCGH) and high-resolution mapping with high-density tiling oligonucleotide arrays. Since complicated biological and experimental processes are often associated with these platforms, data can be potentially contaminated by outliers.ResultsWe propose a penalized LAD regression model with the adaptive fused lasso penalty for detecting CNV. This method contains robust properties and incorporates both the spatial dependence and sparsity of CNV into the analysis. Our simulation studies and real data analysis indicate that the proposed method can correctly detect the numbers and locations of the true breakpoints while appropriately controlling the false positives.ConclusionsThe proposed method has three advantages for detecting CNV change points: it contains robustness properties; incorporates both spatial dependence and sparsity; and estimates the true values at each marker accurately.

The safety of dental amalgam in children

The safety of mercury-containing dental amalgam has been hotly debated for well over a century. Dental exposures from mercury have been suggested as the cause of numerous diseases including multiple sclerosis, autism and many others. Known health effects of mercury exposure include CNS and renal damage. However, these effects have only been shown at occupational or higher levels of exposure, and have not been conclusively shown to be present at levels of mercury exposure consistent with that from dental amalgam fillings. The use of mercury amalgam fillings remains a state-of-the-art treatment for dental caries throughout the world. Although there have been a small number of peer-reviewed reports examining the health effects of dental mercury in children, only very recently have the only randomised, controlled clinical trials (two) of the safety of mercury amalgam been published. The purpose of this review is to discuss the scientific evidence on the safety of the use of mercury-containing dental amalgam as a treatment for dental caries.

High-resolution mapping of DNA copy alterations in human chromosome 22 using high-density tiling oligonucleotide arrays

Deletions and amplifications of the human genomic sequence (copy number polymorphisms) are the cause of numerous diseases and a potential cause of phenotypic variation in the normal population. Comparative genomic hybridization (CGH) has been developed as a useful tool for detecting alterations in DNA copy number that involve blocks of DNA several kilobases or larger in size. We have developed high-resolution CGH (HR-CGH) to detect accurately and with relatively little bias the presence and extent of chromosomal aberrations in human DNA. Maskless array synthesis was used to construct arrays containing 385,000 oligonucleotides with isothermal probes of 45-85 bp in length; arrays tiling the beta-globin locus and chromosome 22q were prepared. Arrays with a 9-bp tiling path were used to map a 622-bp heterozygous deletion in the beta-globin locus. Arrays with an 85-bp tiling path were used to analyze DNA from patients with copy number changes in the pericentromeric region of chromosome 22q. Heterozygous deletions and duplications as well as partial triploidies and partial tetraploidies of portions of chromosome 22q were mapped with high resolution (typically up to 200 bp) in each patient, and the precise breakpoints of two deletions were confirmed by DNA sequencing. Additional peaks potentially corresponding to known and novel additional CNPs were also observed. Our results demonstrate that HR-CGH allows the detection of copy number changes in the human genome at an unprecedented level of resolution.

Proteomic Analysis of Mitochondrial Proteins

The chapter discusses the proteomic analysis of mitochondrial proteins. Mitochondria play a central role in multiple cellular processes. In addition, mitochondrial dysfunction has been implicated in the cause of numerous diseases and disorders, including defects in energy metabolism, alzheimer's and parkinson's diseases, cancer, type 2 diabetes, osteoarthritis, cardiovascular disease, and many drug side effects. A detailed map of the mitochondrial proteome, providing information on identity, function, and protein-protein interactions, would help to understand the complex mechanisms of the cellular function and disease. The rapid evolution of mass spectrometry (MS) based tools in conjunction with protein purification methods, such as 1-D gels, 2-D gels, fractionation techniques, and liquid chromatography allowed the development of mitochondrial protein maps. Numerous researchers have developed mouse models of human mitochondrial diseases by using homologous recombination. The descriptive proteomics techniques, such as mitochondrial protein maps and the functional proteomics techniques, such as (1) protein-protein interactions, (2) post-translational modifications, (3) proteomic expression profiling, and (4) differential protein expression studies using protein arrays, have helped in proteomic analysis. Differentially expressed mitochondrial and associated proteins can be identified by 2-D gel/orthogonal MALDI-TOF peptide mass fingerprinting. Future studies will undoubtedly correlate genomic, proteomic, and array data in an effort to more clearly elucidate the molecular mechanisms of mitochondria and ultimately, the whole cells.

History of tobacco and health

Tobacco comes from plants that are native to the Americas around Peru and Ecuador,where it has been found since prehistoric times. It was brought back to Europe by early explorers where it was adopted by society and re-exported to the rest of the world as European colonization took place. Smoking tobacco in pipes of one sort or other gave way to handmade and then manufactured cigarettes, especially during the First World War. Smoking rates increased dramatically during the 20th century in developed countries until recently and rates are still increasing in underdeveloped countries. An epidemic of smoking-related diseases has followed the prevalence of smoking. Scientific knowledge of the harmful effects of active tobacco smoking has accumulated during the past 60 years since early descriptions of the increasing prevalence of lung cancer. The first epidemiological studies showing an association between smoking and lung cancer were published in 1950. In 1990 the US Surgeon General concluded that smoking was the most extensively documented cause of disease ever investigated but governments worldwide have been ambivalent and slow in taking action to reduce smoking. Tobacco smoking is now agreed to be a major cause of a vast number of diseases and other adverse effects. Since the 1980s passive smoking including exposure in utero has also been implicated as a significant cause of numerous diseases. In response, the tobacco industry has managed to forestall and prevent efforts to control this major health problem.