Viral hepatitis is an infection of the liver caused by the hepatitis virus. There are six different types of viruses called Hepatitis A, B, C, D, E, and G. Hepatitis B virus (HBV) is the main cause of chronic hepatitis, liver cirrhosis, and 80% of hepatocellular carcinoma. Its transmission occurs through sexual, vertical and parenteral routes. The main vehicles for sexual transmission are genital secretions and semen. The best way to prevent hepatitis B is vaccination. A descriptive, cross-sectional study was carried out with the 4th and 5th year students of the Medicine Career - UNNE during the months of August and September 2019, to whom a questionnaire was sent to be completed anonymously to determine the Hepatitis knowledge. 90.78% answered that it is an infectious disease, 88.83% reported that the etiologic agent is a virus and 87.86% answered that the most affected organ is the liver. Furthermore, 90% answered that the transmission route corresponds to the parenteral, sexual and perinatal route. Likewise, 86.41% answered that vaccination is the main form of prevention and 91.26% have been vaccinated against HBV. The level of knowledge about hepatitis B was found to be high. In addition, some risky behaviors were identified such as puncture-cutting accidents and having sex without a barrier method.