Background The impact of testosterone on autism symptoms is still being studied, and the precise mechanisms are not yet fully understood. Objectives Numerous studies have been conducted to investigate the relationship between autism and testosterone levels. The biochemical analysis of testosterone is important to perform for boys, especially for those diagnosed with autism spectrum disorders who also present a high level of aggression. Materials and methods While the exact causes of autism are still unknown, researchers have been studying the relationship between autism and testosterone levels in the body. It is suggested that testosterone may play a role in the higher prevalence of autism spectrum disorder to males compared to females. This has led researchers to investigate whether there is a link between autism and testosteronem. Results Some studies have reported higher levels of testosterone in individuals with autism compared to those without autism. After analyzing the results obtained in our study, higher values of testosterone are observed in children diagnosed with ASD compared to those without ASD who form the control group, but from a statistical point of view there are no significant differences. Some researchers propose that testosterone may have an effect on certain behaviors and characteristics commonly observed in individuals with autism. Studies have shown that children with autism tend to have higher levels of testosterone in their bodies than children without autism. It is important to note that not all children with autism have high levels of testosterone, and not all children with high levels of testosterone have autism. Conclusions These varying results may be attributed to the heterogeneity of autism itself, as it encompasses a wide range of symptoms and characteristics. Early intervention is the key and children with autism can benefit from therapies such as speech therapy, occupational therapy and behavioral therapy. More researches are needed to fully understand the relationship between autism and testosterone.