Causative Agent Of Bacterial Disease Research Articles

Screening of potential isolate candidates probiotic against Aeromonas hydrophila from Boyolali, Indonesia

Mass mortality in catfish culture frequently occurs in Boyolali as a central production due to the outbreak of bacterial diseases. The main causative agent of bacterial disease is Aeromonas hydrophila. This research aimed to find out the bacteria isolates were potential against A. hydrophila. The exploratory method was commenced. Thirty-four isolates were gained from water (SBA01–SBA14) and mud (SBL01 – SBAL20) that were collected from the fish pond of Boyolali Regency, Indonesia with TSA medium. Screening the potential bacteria candidates against A.hydrophila using the sensitivity test that was conducted with in vitro method. Based on the screening results showed that three isolates (SBA14, SBL11, and SBL20) were potential candidates against A. hydrophila. On the basis of sequence 16S rDNA analysis, the result showed that SBA 14, SBL 11 and SBL 20 were closely related to Bacillus flexus, Bacillus subtillis, and Bacillus velezensis respectively.

GntR family regulators of the pathogen of fish tuberculosis Mycobacterium marinum

Mycobacterium marinum is a slow-growing pathogenic mycobacterium. It was first isolated by Aronson in 1926 from fish, fish mycobacteriosis or called fish tuberculosis is the common causative agent of bacterial disease in many species of freshwater and marine fish. M. marinum can infect wild fish, aquaculture and ornamental fish, and it has a close relative of the causative agent of human tuberculosis, Mycobacterium tuberculosis. The recently sequenced genome of M. marinum has been shown to contain several putative GntR regulators. This family named after gluconate regulator has a helix-turn-helix structure. Characterization of transcription regulators and their network is an important step towards the complete understanding of cellular physiology. The regulator of this family shares a similar and conserved N-terminal DNA-binding domain, but has a highly diverse C-terminal effector-binding and oligomerization domain. According to the heterogeneity, we classify the M. marinum GntR family to four subfamilies: FadR, HutC, MocR, and YtrA, and these regulators are encoded by 8, 3, 1 and 1 genes, respectively. Thus this study extends the annotation of M. marinum GntR family proteins, and can help to understand the pathogenic role of this family in M. marinum and facilitate future drug design against this pathogen.

Successful Treatment ofRhodococcus equiPneumonia in an Adult Horse

Successful Treatment of<i>Rhodococcus equi</i>Pneumonia in an Adult Horse