The incidence of trypanosome infections, measured by a Berenil Index in experimental herds of 10 Zebu and 10 N'Dama cattle, was compared with tsetse challenge and with the prevalence of parasitaemia in local N'Dama at three villages in Gambia. Tsetse challenge was more strongly correlated with the incidence of parasitaemia in the Zebu than in the N'Dama. There was a strong correlation between prevalence and incidence of infection in the N'Dama. There was no correlation, however, between prevalence of infection in cattle and tsetse challenge unless the data were offset by 3–5 months. The Berenil Index in the Zebu increased at about twice the rate as in the N'Dama under corresponding levels of challenge. It is concluded that whereas incidence of infection in susceptible animals is best measured independently, it can, under stable conditions, be inferred from an assessment of tsetse challenge.