Abstract With the miniaturization race, organic field-effect transistor (OFET) devices have gained much attention in recent years due to their advantages of flexibility, light weight, solution processability and low manufacturing cost. However, some inorganic or organic materials are eco-unfriendly. Therefore, in this study, the natural biological proteins collagen hydrolysate acts as dielectric material due to the presence of hydroxyl groups. Cationic surfactant hexadecyltrimethylammonium chloride (CTMA) is added into collagen hydrolysate to form a dielectric layer in bio thin film transistor (BioTFT) device. Experimental results highlight that the collagen-CTMA mixture at 3 wt% (Col-CTMA3) has shown an average mobility of 3.36 × 10−2 cm2V−1s−1 and an on/off ratio up to 2.7 × 106.1-aminopyrene is embedded into Col-CTMA3 mixture to form Col-CTMA3-1-aminopyrene (Col-CTMA3-AP) mixture to fabricate bio field-effect transistor (BioFET) type memory device. Furthermore, the optimized addition of 1-aminopyrene in the Col-CTMA3-AP mixture for memory device has provided enhanced performances associated to an average mobility of 1.23 × 10−2 cm2V−1s−1, an on/off ratio up to 5.22 × 105 and a memory window of 13.6 V, as the proportion of 1-aminopyrene increased, the charge-trapping ability of collagen strengthened. Environment effect studies have shown that BioFET memory devices placed under water environment highlight the role of the hydroxyl group for the improved BioFET performance.