Copper Cations Research Articles

English

The research was conducted in tomato-growing lands of Lâpseki, Ezine, Bayramic and Central districts of Canakkale province, Turkey. The aim of the study is to check the suitability of the field for tomato farming and to produce a solution if there is a problem. Disturbed soil samples were taken from 114 points with certain coordinates, at a depth of 0 to 30 cm, and analyses were performed. In the soil samples, texture, soil reaction (pH; 1:2.5), calcium carbonate (CaCO3%), phosphorus (P; kg.ha-1), cation exchange capacity (CEC; meq.100 g-1), iron (Fe; ppm), manganese (Mn; ppm), zinc (Zn; ppm), copper (Cu; ppm) and clay (%) analyses were conducted, and characteristic maps of the region were prepared according to the results of the analyses. Based on these results, the present condition and suitability of the soils were evaluated, and simple statistics along with correlations of the analyzed parameters were examined. For the problems of the area, in low pH areas, it was deemed necessary to apply calcium carbonate (CaCO3) or calcium hydroxide [Ca(OH)2] together with physiological alkaline fertilizers. As per the high pH areas, it was necessary to apply elemental sulfur together with physiological acid fertilizers. It was also concluded that Zn application was necessary for the 43.85% of the area with Zn deficiency. Key words: Efficiency level, nutritional status, plant nutrition, tomato.

Nonwoven flax fibres geotextiles effects on solute heavy metals transport in porous media

ABSTRACTFiltration tests were carried out in laboratory columns filled with crushed sand with and without flax geotextiles to study the transfer and retention of soluble heavy metals. Divalent cations of copper, zinc and lead were simultaneously and continuously injected in filtration columns. Results show that, when geotextiles discs are present the retention of metals in sand is favoured and retention profiles are modified. In addition, and unlike synthetic geotextiles, flax fibres geotextiles contribute to the retention of a significant fraction of the cationic metal pollutants in their own structure. The overall metals retention efficiency of the filter is improved. Competition between cationic metals for adsorption on retention sites occurs in the column in the order Pb > Cu > Zn. Most of the lead is retained in the inlet of the column while copper and even more zinc migrate deeper in the column.

Influence of charge state and active site structure of tetrahedral copper and silver clusters on the methane activation

The process of methane activation on tetrahedral Cu20 and Ag20 clusters was investigated by the density functional theory. In particular, the influence of charge state and structure of clusters on the process of adsorption and dissociation of CH4 molecule was considered. Both neutral and cationic copper clusters were found to promote methane activation better than silver clusters. The study has shown that the coordination number of a metal atom corresponding to the active site has a significant impact on the value of activation energy of methane dissociation. The process of CH4 adsorption is more favorable on the vertex atoms, while CH bond rupture occurs on the edge atoms of the cluster. The presence of a positive charge leads to a significant increase in the value of adsorption energy on the vertex atoms and a decrease in the activation barrier of methane dissociation on the edge sites.

Synthesis and Study of Copper-Containing Polymers of Microcrystalline Cellulose Sulfates from Larch Wood

For the first time, the synthesis of water-soluble copper-containing microcrystalline cellulose sulfates (Сu-MCS) has been performed by the ion exchange method. The composition of the products has been studied by chemical methods and X-ray spectral microanalysis. The copper content in the Сu-MCS samples was 12.6–14.1%. The absence of sodium in the resulting polymer indicates the complete substitution of the sodium cations by the copper cations in the sodium salt of MCC sulfate. The structure of the copper-containing sulfates of microcrystalline cellulose has been confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and electron paramagnetic resonance (EPR). According to the XRD method, Сu-MCS and Na-MCS have an amorphous structure in contrast to the original MCC samples, which have a high degree of crystallinity. The EPR data have demonstrated the formation of a pseudocrystalline structure of the copper-containing salt system in the Сu-MCS samples. As shown by atomic-force microscopy, the surface of the Сu-MCS films consists of homogeneous crystallites, which have a spherical or slightly extended form with the size of about 70 nm. The film surface is quite homogeneous in its phase composition and contains no impurities.

Evaluation of N-Hydroxy-, N-Metoxy-, and N-Acetoxybenzoyl-Substituted Derivatives of Thymine and Uracil as New Substances for Prevention and Treatment of Long-Term Complications of Diabetes Mellitus

New uracil and thymine derivatives, N1-,N3- and N1,N3-(RO-benzoyl)-(1H,3H)-pyrimidine- 2,4-diones, were synthesized (RO- is hydroxy, acetoxy- or methoxy-group). The compounds were studied in a complex of in vitro tests for the ability to inhibit the development of long-term complications of diabetes. Their ability to cleave cross-links of proteins has been evaluated. The most significant ways of pharmacological correction of thrombosis, angio-, nephro-, encephalo-, and cardiopathy, antiglycation, chelating, and antiplatelet activities, have been established. The most active compound in terms of antiplatelet action, N1- hydroxybenzoyluracil, exceeded acetylsalicylic acid by ~44%. In terms of their ability to chelate copper (II) cations, all compounds (with the exception of 1,3-bis(3-hydroxybenzoyl)-(1H,3H)-pyrimidine-2,4-dione that was not not studied in this test) showed the activity, whose IC50 fell in the range between that for pioglitazone (44.1 μM) and pyridoxamine (136.7 μM) comparison drugs. The best antiglycation effect at the 1 mM concentration was observed for N1,N3-bismethoxy- and N1,N3-bisacetoxybenzoyl derivatives of thymine. The maximum activity to cleave cross-links of proteins (C = 1 mM), comparable to that of alagebrium, was established for 1,3-bis(4-methoxybenzoyl)uracil, for which also high rates of other estimated activities were noted. Thus, the N1-,N3- and N1,N3-(RO-benzoyl) derivatives of uracil and thymine are promising basiсs for creating drugs that suppress the development of long-term complications of diabetes.

CuO nanowhiskers: preparation, structure features, properties, and applications

ABSTRACTThis paper presents the results of a detailed study of the structure of nanowhiskers (NWs) of copper oxide formed in the process of thermal oxidation. It is shown that NWs have a bi- or poly-crystalline structure with a growth direction [110]. It is noted that the formation of NWs is facilitated by the cooperation of internal stresses with the defective structure of the underlying coating, and they grow owing to surface diffusion of copper cations from the depth of the coating to the top of the whisker. It is established that NWs have a good sorption and photocatalytic ability that will allow their use in the chemical industry, including the areas of catalysis and photocatalysis.

APPLICATION OF ADSORPTION MODELS FOR DESCRIPTION OF BALANCES IN THE SYSTEMS «CATIONS OF COPPER (II) AND LEAD (II) – NATURAL MATERIALS OF ASTRAKHAN REGION »

I
 In the work there were studied the regularities of extracting copper (II) and lead (II) cations from aqueous solutions with the help of natural sorbents from the Astrakhan region such as the marl of the Baskunchak deposit, the Kamennyy yar deposit and the clay of the Volzhsky deposit. A comparative study was made of the applicability of the adsorption models of Langmuir, Freindlich, Temkin, and Dubinin-Radushkevich to describe the experimental adsorption isotherms of these cations on the surface of aluminosilicates. The constants and parameters of the equations corresponding to these models are determined. A comparison of the regression coefficients shows that the Temkin model is suitable for describing the adsorption equilibria in the "M2+ -clay" systems. The Freundlich model most adequately describes the experimental data on adsorption in the "M2+ -marl/opoka" systems, which reveals the heterogeneous surface of both of these materials with a large number of energetically dissimilar active centers. The regression coefficients found in this case by the Langmuir model are small; the boundary conditions for the description of systems with a monomolecular coating are not observed. The Dubinin-Radushkevich model was also used to describe the process of concentrating lead (II) compounds on the marl surface of the Baskunchak deposit and the Kamennyy Yar deposit. The calculated values of the free energy of adsorption were 11.2 (opoka) and 4.17 kJ/mol (marl). The process of fixing lead cations on these natural sorbents in the Astrakhan region proceeds according to the ion-exchange mechanism. The adsorption activity of Cu2+, Pb2+ ions on the surface of sorbents is confirmed by the calculated values of Gibbs energy and entropy. The endothermic nature of the processes taking place with the participation of the clay of the Volzhsky deposit and the Kamennyy Yar deposit is explained by the considerable energy expenditure for the dehydration of the surface groups of sorbents.
 For citation:
 Dzhigola L.A., Sadomtseva O.S., Shakirova V.V., Kargina K.V., Syutova E.A., Razgovorov P.B., Nagornov R.S. Application of adsorption models for description of equilibria in systems cations of copper (II) and lead (II) – natural materials of Astrakhan region. Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol. 2018. V. 61. N 9-10. P. 105-112

Assessment of the influence of counter ions on biosorption of copper cations in brewer's spent grain - Waste product generated during beer brewing process

The influence that conditioning leached ground barley grain (known as “brewer's spent grain” (BSG)) has on the effectiveness of copper cation sorption was analysed. Saturation with counter ions of functional groups present on the grain surface was carried out in hydrochloric acid, sodium hydroxide, sodium and calcium chloride solutions. For comparison, BSG was also conditioned in demineralised water. Spent barley grain is a by-product of the brewing industry which can be used as a biosorbent in the removal of heavy metals from waste water. Emphasis is placed on the effect of competitive sorption of copper and hydrogen cations and the negative influence of a solution alkalinisation on sorption efficiency. It was demonstrated that the changes of Cu2+ and H+ concentration partition coefficients in the solution and BSG during the sorption process are not monotonic. It was also demonstrated that, compared to raw BSG, conditioning BSG in a hydrochloric acid solution improves sorption efficiency by approximately 40%, which is an important conclusion as regards application. Saturation of functional groups with Na+ and Ca2+ counter ions, which have weak affinity with functional groups, does not improve Cu2+ sorption efficiency; however, it considerably increases the efficiency of hydrogen cations sorption which, in consequence, increases the solution pH. Due to similar chemical characteristics of functional groups, the analyses can be applied in order to optimise sorption processes in other biosorbents. It has been confirmed that ion exchange is the main mechanism of heavy metal sorption in natural sorbents.

Infrared spectroscopy of coordination and solvation in Cu+(C2H4)n (n = 1–9) complexes

Copper-ethylene ion-molecule complexes are produced by laser vaporization of a copper rod in a pulsed supersonic expansion of ethylene and argon. The ions are mass selected and studied via infrared laser photodissociation spectroscopy in the CH stretching region (2600–3400 cm−1). Argon tagging is employed to study the spectra of the n = 1–3 complexes. Spectra of complexes containing four to nine ligands are measured by the elimination of ethylene. Density functional theory calculations are carried out in support of the experimental spectroscopy. The symmetric CH stretches of a free ethylene were found to red shift by about 30 cm−1 in the spectra of Cu+(C2H4) and Cu+(C2H4)2. Cu+(C2H4)3 is found to represent a fully- coordinated copper cation. Its CH stretches are found to blue shift by 10 to 20 cm−1 as a result of steric crowding. In larger complexes, the three-coordinate core ion incorporates additional ethylene "solvent" molecules through cation-π and CH-π interactions. These weak interactions only slightly perturb the vibrations of the external ligands.

An unexpected μ4-oxido-bridged tetranuclear Cu(II) inverse coordination complex of a heptadentate bis(pyrazolyl)methane-based ligand: Synthesis, structure, spectroscopic properties, and catecholase activity

A new tetranuclear copper(II) complex, {But-C6H2(O)[CH = N-CH2-CH(pz)2]2}2Cu4(μ4-O)(OAc)4 (L2Cu4(μ4-O)(OAc)4, pz = pyrazolyl ring) has been prepared from the condensation reaction of 4-tert-butyl-2,6-diformylphenol with two equivalents of bis(pyrazolyl)ethanamine moieties in the presence of two equivalents of copper(II) acetate. The complex has been characterized by X-ray diffraction studies in solid state, as well as by UV–Vis, IR, and ESI spectroscopies. The structure of this inverse coordination complex consists of a central oxygen ion surrounded by four copper(II) cations residing in the corners of a distorted tetrahedron; the remaining coordination sites of the Cu2+ ions are filled by imine donors and acetate counter-ions, leaving the bis(pyrazolyl)methane donor groups uncoordinated to the copper(II) centers. In contrast to the solid-state structure, this compound was found to be dinuclear in solution, LCu2(OAc)2(OH). The complex catalyzes the oxidation of 3,5-di-tert-butylcatechol (DTBC) and 4-tert-butylcatechol (TBC) to their corresponding quinones. The catalytic mechanism was investigated using DTBC as a substrate, and it was found that the oxidation reaction occurs via two different pathways, one involving molecular oxygen and the other hydrogen peroxide as oxidants.

Two Cu(II) complexes of 1,2,4-triazole fungicides with enhanced antifungal activities

Two Cu(II) complexes, namely, [CuL12(OAc)2]·MeOH 1 and [CuL22(OAc)2] 2 (OAc = CH3COO, acetate), were synthesized based on two 1,2,4-triazole fungicides of paclobutrazol (L1) and diniconazole (L2). The crystal structural analysis shows that the two obtained complexes have similar structures and both of them adopt octahedral coordination mode. The Cu(II) atoms in both the complexes lie on a crystallographic inversion center and are coordinated with two triazole groups and two acetate anions. To screen effective fungicides, the two complexes were investigated for antifungal activities against four selected fungi using mycelial growth rate method. The results demonstrate that the toxicity of complex 2 was 6.96–23.39 times greater than that of complex 1, since complex 2 possesses both CC linkage and 2,4-dichlorobenzene moiety, and shows higher synergy levels for the molecular-level mixture of copper cation and L2. The results also show that both the synthesized complexes showed superior activities of 1.20–5.94 times to those of their ligands. The DFT calculation results of the title complexes and their ligands reveal that the complexes have a new active site of metal cation, smaller HOMO–LUMO energy gaps and lower polarity than their ligands, which should be responsible for the enhanced bioactivities after complexation.

First-Principles Comparison of Proton and Divalent Copper Cation Exchange Energy Landscapes in SSZ-13 Zeolite

The relative proximity of Al atoms substituted in zeolite lattices is an important parameter that influences both hydrothermal stability and catalytic function, but the underlying chemistry that governs Al site proximity is not well understood. Here, we examine relationships between exchanged countercations and different Al–Al arrangements in a chabazite (SSZ-13) zeolite lattice. We report periodic supercell density functional theory (DFT) calculations for structures and energies of SSZ-13 lattices with systematically enumerated and varied Al–Al proximity, both charge-uncompensated and charge-compensated by either proton pairs (H+/H+) or divalent copper cations (Cu2+). Al–Al interactions are electrostatically repulsive without charge compensation, but the relative energies of certain Al–Al site arrangements change upon compensation by countercations. Al–Al interactions are uniformly attractive when compensated by H+/H+ pairs but are attractive at long and repulsive at short Al–Al distances when compensate...

Synthesis, spectroscopy, electrochemistry, and molecular structure of tetrakis{(E)-2-((pyridin-2-ylimino)methyl)phenolato}(hydroxido)0.5(nitrato)1.5-tetracopper(II) nitrate hydroxide

Reaction between (E)-2-((pyridin-2-ylimino)methyl)phenol (HL) and copper(II) nitrate provides tetrakis{(E)-2-((pyridin-2-ylimino)methyl)phenolato}(hydroxido)0.5(nitrato)1.5-tetracopper(II) nitrate hydroxide, [(CuL)4(NO3)1.5(OH)0.5](NO3)(OH) (1). ESI-mass spectra show the ion peaks for the dinuclear species at m/z 565 for [(CuL)2(HCO2)]+ and 521 for [(CuL)2+H]+ and the mononuclear species at m/z 260 for [(CuL)]+. Vibrational spectra show very strong bands at 1604/1546 cm−1 for ν(C = N/C = C) and at 1384, 1351 cm−1 for ν(NO3–). Cyclic voltammograms demonstrate an irreversible redox processes for the Cu(II)/Cu(I) and Cu(I)/Cu(0) couples in acetonitrile. X-ray molecular structure determination explores the formation of a cationic tetranuclear copper(II)-complex, in which a deprotonated ligand molecule chelates to one copper ion with the phenolate-O and imino-N atoms. In addition, a phenolate-O atom bridges between two neighboring copper ions and a pyridine-N atom coordinates to a third copper ion, so that each ligand bridges among three copper ions in a κ2N,O:κO:κN' coordination sphere. Thus, the four copper ions and four chelating-bridging ligands assemble primarily into a cationic [(CuL)4]4+ complex. The two copper ions are further coordinated by either a nitrate anion (75% occupancy) or a hydroxide anion (25% occupancy) and form the core of a tetranuclear [(CuL)4(NO3)1.5(OH)0.5]2+ cation.

Relevance of Copper and Organic Cation Transporters in the Activity and Transport Mechanisms of an Anticancer Cyclometallated Gold(III) Compound in Comparison to Cisplatin.

The molecular mechanisms of toxicity and cellular transport of anticancer metallodrugs, including platinum-based agents, have not yet been fully elucidated. The aim of our study was to investigate the relevance of copper transporters (CTR1 and ATP7A/B), organic cation transporters (OCT2) and the multidrug and toxin extrusion proteins (MATE) in the intracellular accumulation of a novel organometallic cytotoxic Au(III) compound in cancer cells in comparison to cisplatin. Specifically, the synthesis and characterization of the gold complex [Au(pyb-H)(PPh2Ar)Cl]PF6 (PPh2Ar = 3-[4-(diphenylphosphino)phenyl]-7-methoxy-2H-chromen-2-one] (1), featuring a coumarin ligand endowed with “smart” fluorescence properties, have been achieved. Initially, the cytotoxic effects of both cisplatin and 1 were studied in a small panel of human cancer cells, and against a non-tumorigenic cell line in vitro. Thus, the human ovarian cancer cell line A2780 and its cisplatin resistant variant A2780cisR, were selected, being most sensitive to the treatment of the gold complex. Co-incubation of the metallodrugs with CuCl2 (a CTR1 substrate) increased the cytotoxic effects of both the Au(III) complex and cisplatin; while co-incubation with cimetidine (inhibitor of OCT2 and MATE) showed some effect only after 72 h incubation. ICP-MS (Inductively Coupled Plasma Mass Spectrometry) analysis of the cell extracts showed that co-incubation with CuCl2 increases Au and Cu accumulation in both cancer cell lines, in accordance with the enhanced antiproliferative effects. Conversely, for cisplatin, no increase in Pt content could be observed in both cell lines after co-incubation with either CuCl2 or cimetidine, excluding the involvement of CTR1, OCT2, and MATE in drug accumulation and overall anticancer effects. This result, together with the evidence for increased Cu content in A2780 cells after cisplatin co-treatment with CuCl2, suggests that copper accumulation is the reason for the observed enhanced anticancer effects in this cell line. Moreover, metal uptake studies in the same cell lines indicate that both 1 and cisplatin are not transported intracellularly by CTR1 and OCT2. Finally, preliminary fluorescence microscopy studies enabled the visualization of the sub-cellular distribution of the gold compound in A2780 cells, suggesting accumulation in specific cytosolic components/organelles.

Supramolecular metal–organic frameworks based on neutral and cationic copper(II) α-hydroxycarboxylate complexes with 2,2′-dipyridylamine

The reaction of CuCO3·Cu(OH)₂ with three different α-hydroxyacids (H2L′) and the nitrogen donor coligand 2,2′-dipyridylamine (dipyam) has produced five new discrete copper(II) complexes. Two kind of compounds have been isolated: neutral complexes of formula [Cu(H2L′)2(dipyam)] and cationic [Cu(H2L′)(dipyam)2](H2L′) complexes. Four of these compounds have been studied by single-crystal X-ray diffraction and the analysis of their molecular crystal structures were also performed by the analysis of their Hirshfeld surfaces. The thermal behaviour has also been investigated.

Molecular Conjugation for the "Diazido Probe" Method

Target identification of screening hit compounds with unknown mechanisms of action obtained from chemical libraries by phenotypic assays has played an important role in the development of innovative drugs that work based on novel mechanisms. To improve the usability of the target identification based on the photoaffinity labeling method, we have studied the "diazido probe" method, wherein a photoreactive aromatic azido and relatively photostable aliphatic azido groups are sequentially used for photoreaction and introduction of a latent detectable tag via the Staudinger ligation or click reactions, and related synthetic methods that enable expeditious preparation of molecular probes. To facilitate the development of diazido probes, we established short synthetic routes to diazido building blocks with different connectable groups based on sequential iridium-catalyzed C-H borylation and copper-catalyzed azidation of 1,3-disubstituted benzenes, and subsequent diverse functional group transformations leaving the azido groups untouched. To improve the utility of click chemistry for efficient introduction of a latent detectable tag, we developed a transient protection method of cyclooctynes from cycloaddition with an azide by 1 : 1 complexation with a cationic copper(I) salt. Application of this protection method using a cationic copper salt to a cyclooctyne bearing a terminal alkyne allowed for the selective click conjugation with an azide at the terminal alkyne moiety, which rendered functionalized cyclooctyne derivatives easily synthesizable.

Alkyl Sulfate Compounds with Cationic Metal Complexes and Some Organic Reagents as Active Membrane Components of Potentiometric Sensors

A review of publications for the last 20 years devoted to the purposeful search for new ionophores ensuring the reliable, reproducible, and selective determination of analytes. The synthesis of new ionophores and optimization of sensor designs in combination with their simplicity, availability, and low cost allow the modernization of present-day sensor technologies. The constantly growing interest in metal complexes with various organic reagents favors the expansion of the areas of their use, in particular, for the development of potentiometric sensors. Potentiometric sensors are a promising tool of the quality control of surfactants (SAS) and their presence in various samples. It was shown that the introduction of alkyl sulfate compounds with cationic copper(II) complexes of some organic reagents (pyridine, 1,10-phenanthroline, 2,2'-dipyridyl) into the composition of membranes ensures the creation of sensors selective to ionic surfactants with improved electroanalytical characteristics.

Selectivity of Ion Exchange on Iminocarboxyl Resin and the Hydration Energy of Its Ionic Forms

Characteristics of the hydration of macroporous iminodicarboxyl ion-exchange resin in the protonated, sodium, copper, and nickel forms are determined, and the Gibbs energy of hydration and its change upon transitioning from the initial ionic form to the ion-sorbed form are calculated. The way of calculating the Gibbs energy of the interfacial transfer of a solvent during the ion-exchange process is substantiated. It is established that the selectivity of an ion-exchange resin in the functional form toward the extracted transition-metal cations depends not only on the mean amount of solvent per functional group of the polyampholite, but also on the change in sorbent hydration during the ion exchange. The contribution determining the selectivity of copper(II) and nickel(II) cations toward iminodicarboxyl ion-exchange resin is an ion-exchange component with the formation of ionite complexes. In addition, the change in hydration (being an energetically advantageous process) facilitates sorption as a whole.

Ethylene adsorption onto natural and transition metal modified Chilean zeolite: An operando DRIFTS approach

Abstract Ethylene is a plant growth regulator that induces accelerated softening and ripening of fruits during transport and storage. Among the most applied methods for ethylene control, adsorption appears as a cheap and efficient technique. In this work, the effect of the incorporation of transition metals into natural Chilean zeolite on ethylene adsorption is investigated. Natural zeolite mainly composed of clinoptilotite and mordenite is modified using copper and zinc nitrate solutions and calcined under oxygen flow at 623 K, generating different transition metal modified zeolites. Parent and modified zeolites were characterised by X-ray diffraction, X-ray fluorescence spectroscopy and nitrogen adsorption. Zeolite surface modifications were assessed by diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). Experimental results reveal the incorporation of Cu2+ and Zn2+ as new compensating cations into the zeolite framework. Ethylene adsorption isotherms show an enhancement on the adsorption capacity of Cu-exchanged zeolite. This result is not only associated to the higher micropore surface area of this sample, but also to the higher affinity of ethylene molecules to copper cations incorporated on this zeolite. DRIFTS operando experiments of ethylene adsorption in the absence and in the presence of moisture reveal a competitive mechanism of water and ethylene molecules toward hydroxyl sites. Si-OH-Al and Si-OH sites are rapidly occupied with water, reducing the adsorption of ethylene.

Scrutiny of interference effect of ions and organic matters on water treatment using supported nanoscale zero-valent iron

Nanoscale zero-valent iron (nZVI) supported on heat-modified activated charcoal (nFe(0)/AC) can improve nitrate reduction and phosphate adsorption regarding reaction conversion, adsorption capacity, and kinetics. It is more effective in terms of mobility and stability than bare nZVI. Both nZVI and its supported type were synthesized, characterized, and examined in interference studies for applications in environmental remediation technologies. Solutions of 45.18 mg nitrate–N/L, 50 mg phosphorus/L, and a mixture of them were treated using nZVI and nFe(0)/AC in municipal wastewater body and also deionized water body in the coexistence of anions (phosphate and sulfate), cations (cuprous and cupric), organic matters (humic acid), and hardness (calcium carbonates) at different concentrations. Results showed the significant impact of interference on nitrate reduction and superiority of nFe(0)/AC by ca. 27% to ca. 183% increase in treatment efficiency over nZVI. Anions were easily attracted to the surface of nano-iron particles resulting in a negative intervening effect. Hardness and contaminants of municipal wastewater provided a negative impact and significantly interfered with nitrate removal, while organic matters had a lower negative interference compared to others. On the contrary, copper cations could improve removal efficiency until complete elimination of nitrate. The experimental data were best-fitted to a kinetic rate model that combined the pseudo-first-order rate with the deactivation rate arising from the passivation of interfering substances on the surface of nZVI-based materials. The value of parameters of this rate equation could estimate the degree and type of interference occurring during nitrate decontamination.