The automatic identification of plant species using unmanned aerial vehicles (UAVs) is a valuable tool for ecological research. However, challenges such as reduced spatial resolution due to high-altitude operations, image degradation from camera optics and sensor limitations, and information loss caused by terrain shadows hinder the accurate classification of plant species from UAV imagery. This study addresses these issues by proposing a novel image preprocessing pipeline and evaluating its impact on model performance. Our approach improves image quality through a multi-step pipeline that includes Enhanced Super-Resolution Generative Adversarial Networks (ESRGAN) for resolution enhancement, Contrast-Limited Adaptive Histogram Equalization (CLAHE) for contrast improvement, and white balance adjustments for accurate color representation. These preprocessing steps ensure high-quality input data, leading to better model performance. For feature extraction and classification, we employ a pre-trained VGG-16 deep convolutional neural network, followed by machine learning classifiers, including Support Vector Machine (SVM), random forest (RF), and Extreme Gradient Boosting (XGBoost). This hybrid approach, combining deep learning for feature extraction with machine learning for classification, not only enhances classification accuracy but also reduces computational resource requirements compared to relying solely on deep learning models. Notably, the VGG-16 + SVM model achieved an outstanding accuracy of 97.88% on a dataset preprocessed with ESRGAN and white balance adjustments, with a precision of 97.9%, a recall of 97.8%, and an F1 score of 0.978. Through a comprehensive comparative study, we demonstrate that the proposed framework, utilizing VGG-16 for feature extraction, SVM for classification, and preprocessed images with ESRGAN and white balance adjustments, achieves superior performance in plant species identification from UAV imagery.