Summary1. Invertebrate communities at 187 least impacted streams in the Manawatu–Wanganui region of New Zealand were sampled between February and May 2000 to investigate the relative influence of catchment and reach scale environmental influences on community structure.2. Of the 138 biological (fish and periphyton), local habitat and catchment scale descriptors used to examine invertebrate community patterns, alkalinity and conductivity were the most consistently influential predictors.3. Of the 52 geographical information system (GIS)‐derived catchment variables (catchment geology, catchment land use, rainfall and topography) only per cent catchment in pasture, indigenous forest, coastal sand, crushed argillite and wind blown sand were associated with any measures of the invertebrate communities.4. Grouping of communities based on GIS data in general, did not generate distinct community types. Groupings based on river catchment, conductivity and alkalinity however, did produce distinct communities.5. Streams with very low alkalinity were dominated by Ephemeroptera, Plecoptera and Trichoptera that were gradually replaced by Mollusca, Crustacea and Chironomidae as alkalinity increases.6. Habitat characteristics measured at the scale of the reach were more closely linked with measures of invertebrate community structure than any GIS derived variables or river classifications.