Catalytic Oxidation Of Glucose Research Articles

AuNPs@MoS2 functionalized copper metal organic framework for boosting electrocatalytic glucose oxidation

A novel non-enzyme glucose sensor based on functionalized copper metal–organic framework composite material, AuNPs@MoS2/Cu-MOF, was developed for rapid glucose analysis. Cu-MOF nanomaterial was prepared via a hydrothermal method, followed by the surface loading of core–shell structured AuNPs@MoS2 to synthesize AuNPs@MoS2/Cu-MOF composite material. Consequently, a novel gold nanoparticle @ molybdenum disulfide/copper metal–organic framework (AuNPs@MoS2/Cu-MOF/GCE) non-enzyme glucose sensor was constructed. Scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray powder Diffraction (XRD) was used to verify that the non-enzyme glucose sensor had been constructed effectively. Traditional electrochemical characterization methods were employed to study the electrocatalytic performance of the AuNPs@MoS2/Cu-MOF/GCE sensor and its catalytic oxidation of glucose. The experimental results demonstrated a good linear correlation between the concentration of glucose and the peak current within the range of 0.05–14.85 mM, with a detection limit of 16.7 μM. The AuNPs@MoS2/Cu-MOF/GCE sensor exhibited rapid electrochemical response to glucose (within 0.5 s), along with long-term stability and excellent selectivity. This is because Cu(II) in Cu-MOF can serve as active sites for the electrocatalytic oxidation of glucose, while the core–shell structure of AuNPs@MoS2 with excellent conductivity can lower the energy barrier in the reaction, further accelerating this catalytic reaction. The AuNPs@MoS2/Cu-MOF/GCE sensor is cost-effective, easy to operate, and suitable for accurate rapid quantification of glucose.

Microvolumetric determination of thrombomodulin based on competitive immunoreaction using a portable glucometer.

A new method of reducing the amount of reagent and sample for determination of thrombomodulin (TM) was developed based on competitive immunoreaction using a portable glucometer (PGM). Two types of nanocomposites, TM protein-modified magnetic nanoparticles (MNPs-TM) and TM antibody-/glucose oxidase-modified gold nanoparticles (Ab-GNPs-GOx), were prepared. Their binding product, MNPs-TM-Ab-GNPs-GOx, in the microvolumetric solution was used to catalyze the oxidation of glucose, leading toa decline of the glucose content. The TM-involved competitive immunoreaction had a negative effect on the generation of MNPs-/GNPs-based nanocomposites and inhibited the catalytic oxidation of glucose. The glucose content difference in the microvolumetric solution, which was revealed by a PGM, was in proportion to the logarithm of the TM concentration from 25ngmL-1 to 2.5μgmL-1. The limit of detection was 5.7ngmL-1. Microvolumetric solution and a PGM were used in the measurement, which overcame some deficienciesof classical methods in chemo/biosensing, for example, special instrument, complicated measurement procedure, and high cost.

Enzyme Engineering to Produce SWCNT Nanosensors as a Generalizable Platform for Biochemical Imaging and Sensing

Single-walled carbon nanotubes (SWCNT) stand out as attractive candidates for near-infrared (nIR) fluorescent imaging and chemical sensing. However, to render SWCNT chemically-selective, rational design of the surface corona is required to achieve sufficient sensitivity and selectivity to the target analyte. Recently, we reported on a facile sonication-based generation of glucose oxidase (GOx)-SWCNT conjugate for glucose detection [1]. Notably, we found that the analyte response mechanism does not involve catalytic oxidation of glucose, which motivated us to develop nanosensors using catalytically inactive GOx for enhanced reversibility and biocompatibility. In this study, we study the generalizability of our approach in producing SWCNT-based nanosensors based on 1- facile direct probe tip sonication, and 2- with engineered enzymes, showing the potential applicability of the sonication-based approach as a generalizable strategy for generating rationally designed nanosensors capable of detecting and imaging various analytically-relevant biomolecules.In this work, to test the generalizability of generating enzyme-SWCNT nanosensors, we generated conjugates by facile direct sonication of SWCNT with following enzymes: GOx, choline oxidase (ChOx), horseradish peroxidase (HRP), acetylcholineesterase, tyrosinase, cholesterol oxidase (CholOx), lactate oxidase, alcohol oxidase, xanthine oxidase, and galactose oxidase. Remarkably, six out of nine enzymes yielded stable suspensions of enzyme-SWCNT conjugates following probe-tip sonication, all of which successfully worked as nanosensors to detect their corresponding analytes. These nanosensors, in particular HRP-SWCNT and CholOx-SWCNT, exhibited excellent responses to their targets, with a maximum ΔF/F 0 of up to 300 % and 100 %, respectively. H2O2 is known to quench SWCNT fluorescence, and H2O2 nanosensors previously developed with SWCNT exhibited a negative fluorescence modulation as the sensor output with some challenges towards nanosensor selectivity. We highlight that our approach enables HRP-SWCNT nanosensor generation, which provides a strong ΔF/F 0 = 300 % and instantaneous turn-on response towards H2O2 within 2 s. Next, we sought to show that enzyme inactivation can produce SWCNT-based nanosensors that detect analytes without analyte consumption. To do so, we use ChOx as a model system to show that recombinant enzymes can be used as a generalizable approach to generating catalytically-inactive nanosensors. As expected, nanosensors prepared with mutant-ChOx exhibited responses comparable to those prepared with native ChOx, but without consuming the analyte or producing toxic byproducts such as H2O2. Thus, sonication-based physisorption of engineered enzymes to SWCNTs holds the potential to facilitate rapid nanosensor generation capable of detecting various biologically relevant molecules with reversibility and biocompatibility, thereby motivating their use in in vivo applications.[1] Nishitani, S., Tran, T., Puglise, A., Yang, S. & Landry, M. Engineered Glucose Oxidase-Carbon Nanotube Conjugates for Tissue-Translatable Glucose Nanosensors. Angew. Chem. Int. Ed Engl. e202311476 (2023)

V2O5/TiO2 Catalyst for Catalytic Glucose Oxidation to Formic Acid in Batch Reactor: Vanadium Species Nature and Reaction Conditions Optimization

V2O5/TiO2 Catalyst for Catalytic Glucose Oxidation to Formic Acid in Batch Reactor: Vanadium Species Nature and Reaction Conditions Optimization

An aptasensor for chloramphenicol determination based on dual signal output of photoelectrochemistry and colorimetry

In the present work, we developed an aptasensor to determine chloramphenicol (CAP) based on the dual signal output of photoelectrochemistry (PEC) and colorimetry. The Fe3+-doped porous tungsten trioxide was prepared by sol-gel method and coated on the ITO conductive glass to form ITO/p-W(Fe)O3. After assembling the captured DNA (cDNA) and the aptamer of CAP (apt) successively, the constructed ITO/p-W(Fe)O3-cDNA/apt aptasensor exhibited excellent photocurrent response under visible light irradiation in the presence of glucose, which provided the feasibility for PEC measurement with high sensitivity. In the presence of CAP, the apt left the ITO/p-W(Fe)O3 surface and AuNPs linked on the probe DNA would be assembled on it, which led to the decrease of photocurrent. Thanks to the oxidase-mimic catalytic performance of AuNPs and the recycling catalytic hydrolysis by exonuclease I, the measurement signal of the aptasensor could be amplified significantly, and the photocurrent decrease of the aptasensor was linearly related to the concentration of CAP in the range of 1.0 pM–10.0 nM and low detection limit was 0.36 pM. Meanwhile, the H2O2 produced from catalytic oxidation of glucose could oxidize TMB to blue oxTMB under HRP catalysis, which absorbance at 652 nm was linearly related to the concentration of CAP in the range of 5.0 pM–10.0 nM and low detection limit was 1.72 pM. Therefore, an aptasensor that determine CAP in real samples was successfully constructed with good precision of the relative standard deviation less than 5.7 % for PEC method and 7.3 % for colorimetric method, which can meet the analysis needs in different scenarios.

Ultrasonication‐Assisted Preparation of Au‐Pt/ZrO2 Catalysts for the Selective Base‐Free Oxidation of Glucose to Glucaric Acid

AbstractSelective catalytic oxidation of glucose to glucaric acid is still challenging due to the potential formation of a large range of by‐products. ZrO2 supported AuPt catalysts are very efficient for this reaction under base‐free conditions, in aqueous phase. Different preparation methods were tested and the catalysts synthesized by co‐impregnation using NaBH4 as reductant gave the most promising results. We demonstrated that the presence of a pure AuPt alloy is critical for the selective formation of glucaric acid. We have shown that when ultrasonication was used during preparation, less by‐products were formed, increasing significantly final yield of glucaric acid, up to 71 %. The Au/Pt ratio affects the catalytic results and an optimal ratio around 1.1 was determined. Finally, the catalyst is stable up to 196 h in continuous reactor.

Lysine aggregates‐based nanostructured antimicrobial peptides for cariogenic biofilm microenvironment‐activated caries treatment

AbstractDental caries is one of the most prevalent and costly biofilm‐induced oral diseases that causes the deterioration of the mineralized tooth tissue. Traditional antimicrobial agents like antibiotics and antimicrobial peptides (AMPs) struggle to effectively eradicate bacteria in biofilms without eliciting resistance. Herein, we demonstrate the construction of FeOOH@Fe‐Lysine@Au nanostructured AMPs (nAMPs) distinguished by their AMP‐like antibacterial activity and self‐producing reactive oxygen species (ROS) capacity for caries treatment. On the one hand, FeOOH@Fe‐Lysine@Au nAMPs can catalyze glucose oxidation to generate ROS within the cariogenic biofilm microenvironment, resulting in the disintegration of the extracellular polymeric substance matrix and the exposure of bacteria. On the other hand, FeOOH@Fe‐Lysine@Au nAMPs can attach to bacterial surfaces via electrostatic attractions, proceeding to damage membranes, disrupt metabolic pathways, and inhibit protein synthesis through the aggregated lysine and the generated ROS. Based on this antibacterial mechanism, FeOOH@Fe‐Lysine@Au nAMPs can effectively eradicate Streptococcus mutans and its associated biofilm, significantly impeding the progression of dental caries. Given the straightforward and cost‐efficient preparation of FeOOH@Fe‐Lysine@Au nAMPs compared to AMPs that require specific sequences, and their minimal adverse impacts on gingival/palatal tissues, major organs, and oral/gut microbiomes, our research may promote the development of novel therapeutic agents in dental health maintenance.

Au nanoparticle-embellished UiO-66 on reduced graphene oxide as a non-enzymatic electrocatalyst at a remarkably low oxidation potential for glucose oxidation and sensing.

Au nanoparticle-embellished metal-organic framework UiO-66 on reduced graphene oxide (Au/UiO-66/rGO) was synthesized. Au/UiO-66/rGO displayed strong electrocatalytic activity for oxidation of glucose in alkaline solution at a remarkably low oxidation potential of +0.20 V vs. Ag/AgCl. Au nanoparticles played a paramount role in the catalytic oxidation of glucose at the electrode, while both rGO and UiO-66 can significantly enhance the current responses to glucose. The resulting non-enzymatic glucose sensor exhibited a wide range of linear response, high sensitivity and selectivity for the determination of glucose. The sensor was successfully applied for the determination of glucose in honey products.

Boosted Catalytic Performance of Ni2Co2@T-PMo@ZIF-67 for Glucose Oxidation in a Direct-Glucose Fuel Cell

In this study, we report on how to design efficient catalysts for glucose oxidation via the transitional metal doping of nanohybrids of polyoxometalates (POMs) and metal-organic frameworks (MOFs). ZIF-67, a cobalt-based MOF, as well as phosphomolybdic acid (PMo), were used as precursors for the fabrication of pyrolyzed PMo@ZIF-67 (T-PMo@ZIF-67). A different amount of Ni2+ was doped into PMo@ZIF-67 to produce NixCoy@T-PMo@ZIF-67. Among them, Ni2Co2@T-PMo@ZIF-67 had the best performance. The power density of the fuel cell that used Ni2Co2@T-PMo@ZIF-67 as an anode catalyst was 3.76 times that of the cell that used active carbon as an anode catalyst. SEM and EDS mapping results indicate that Ni2Co2@T-PMo@ZIF-67 has a spherical structure and rough surface, and elements such as cobalt, nickel, and molybdenum are evenly distributed. XRD characterization indicates that Co3O4, CoMoO4, CoNiO4, and MoNiO4 co-exist in the composites. It is supposed that Co2+, Mo6+, and Ni2+ in the composites may have synergistic effects on the catalytic oxidation of glucose.

Rapid Synthesis of Trimetallic Nanozyme for Sustainable Cascaded Catalytic Therapy via Tumor Microenvironment Remodulation.

Tumor microenvironment (TME)-responsive nanozyme-catalyzed cancer therapy shows great potential due to its specificity and efficiency. However, breaking the self-adaption of tumors and improving the sustainable remodeling TME ability remains a major challenge for developing novel nanozymes. Here, a rapid method is developed first to synthesize unprecedented trimetalic nanozyme (AuMnCu, AMC) with a targeting peptide (AMCc), which exhibits excellent peroxidase-like, catalase-like, and glucose oxidase-like activities. The released Cu and Mn ions in TME consume endogenous H2 O2 and produce O2 , while theAMCccatalyzes glucose oxidation reaction to generate H2 O2 and gluconic acid, which achieves the starvation therapy by depleting the energy and enhances the chemodynamic therapy effect by lowering the pH of the TME and producing extra H2 O2 . Meanwhile, the reactive oxygen species damage is amplified, as AMCc can constantly oxidize intracellular reductive glutathione through the cyclic valence alternation of Cu and Mn ions, and the generated Cu+ elevate the production of ·OH from H2 O2 . Further studies depict that the well-designed AMCc exhibits the excellent photothermal performance and achieves TME-responsive sustainable starvation/photothermal-enhanced chemodynamic synergistic effects in vitro and in vivo. Overall, a promising approach is demonstrated here to design "all-in-one" nanozyme for theranostics by remodeling the TME.

Engineered Glucose Oxidase‐Carbon Nanotube Conjugates for Tissue‐Translatable Glucose Nanosensors

AbstractContinuous and non‐invasive glucose monitoring and imaging is important for disease diagnosis, treatment, and management. However, glucose monitoring remains a technical challenge owing to the dearth of tissue‐transparent glucose sensors. In this study, we present the development of near‐infrared fluorescent single‐walled carbon nanotube (SWCNT) based nanosensors directly functionalized with glucose oxidase (GOx) capable of immediate and reversible glucose imaging in biological fluids and tissues. We prepared GOx‐SWCNT nanosensors by facile sonication of SWCNT with GOx in a manner that—surprisingly—does not compromise the ability of GOx to detect glucose. Importantly, we find by using denatured GOx that the fluorescence modulation of GOx‐SWCNT is not associated with the catalytic oxidation of glucose but rather triggered by glucose‐GOx binding. Leveraging the unique response mechanism of GOx‐SWCNT nanosensors, we developed catalytically inactive apo‐GOx‐SWCNT that enables both sensitive and reversible glucose imaging, exhibiting a ΔF/F0 of up to 40 % within 1 s of exposure to glucose without consuming the glucose analyte. We finally demonstrate the potential applicability of apo‐GOx‐SWCNT in biomedical applications by glucose quantification in human plasma and glucose imaging in mouse brain slices.

Engineered Glucose Oxidase-Carbon Nanotube Conjugates for Tissue-Translatable Glucose Nanosensors.

Continuous and non-invasive glucose monitoring and imaging is important for disease diagnosis, treatment, and management. However, glucose monitoring remains a technical challenge owing to the dearth of tissue-transparent glucose sensors. In this study, we present the development of near-infrared fluorescent single-walled carbon nanotube (SWCNT) based nanosensors directly functionalized with glucose oxidase (GOx) capable of immediate and reversible glucose imaging in biological fluids and tissues. We prepared GOx-SWCNT nanosensors by facile sonication of SWCNT with GOx in a manner that-surprisingly-does not compromise the ability of GOx to detect glucose. Importantly, we find by using denatured GOx that the fluorescence modulation of GOx-SWCNT is not associated with the catalytic oxidation of glucose but rather triggered by glucose-GOx binding. Leveraging the unique response mechanism of GOx-SWCNT nanosensors, we developed catalytically inactive apo-GOx-SWCNT that enables both sensitive and reversible glucose imaging, exhibiting a ΔF/F0 of up to 40 % within 1 s of exposure to glucose without consuming the glucose analyte. We finally demonstrate the potential applicability of apo-GOx-SWCNT in biomedical applications by glucose quantification in human plasma and glucose imaging in mouse brain slices.

Colorimetric and fluorescent detection of glucose based on Fe7S8 nanosheets catalyzed oxidation of o-phenylenediamine

Fe7S8 nanosheets (Fe7S8 NSs) was prepared from ferrous sulfate and thiourea, showing peroxidase-like activity and enabling the oxidation of o-phenylenediamine (OPD) in the presence of H2O2 to produce 2,3-diaminophenol (DAP) with strong absorption and fluorescence peaks at 450 and 560 nm, respectively. Furthermore, both the level of absorbance at 450 nm (A450) and the intensity of fluorescence at 560 nm (F560) depended on the concentration of H2O2. As a result, a dual response (colorimetry and fluorescence) method for the assay of H2O2 was established. Due to the fact that H2O2 can be produced from glucose oxidase catalyzed glucose oxidation, A450 and F560 were further used for indirectdetermination of glucose. The results indicated that the absorbance and fluorescence responses were closely related to glucose concentration in the range of 1.0–100 μM and 1.0––70 μM with the detection limit of 0.83 and 0.52 μM, respectively. To verify its practicality, the method has been used for determining glucose in human serum samples with recoveries ranging from 92.2 to 106.2 %, suggesting its promising potential applications.

Glucose oxidase-like Co-MOF nanozyme-catalyzed self-powered sensor for sensitive detection of trace atrazine in complex environments

The self-powered sensor (SPS) is a sensor method that does not require the external power source and has the potential for portable detection of environmental contaminants. In this work, for the first time, a biomolecule-free SPS for detection of ultra-trace triazine endocrine disruptor atrazine (ATZ) with high sensitivity and selectivity is constructed using a glucose oxidase (GOD)-like cobalt metal-organic framework (Co-MOF) nanozyme-modified high-performance anode and a molecularly imprinted cathode. By modulating the size and morphology of the prepared materials, Co-MOF nanozyme with superior GOD-like property (Michaelis constant Km = 15.8 mM) has been obtained and modified at the anode to catalyze glucose oxidation with high efficiency and provide energy continuously and stably for the SPS. The separation mode of anodic energy supply-cathodic recognition ensures the recognition effect without affecting the catalytic characteristic of Co-MOF and the output signal of the SPS. The designed SPS has a wide linear range of 1 pM–100 nM and a detection limit as low as 0.65 pM, as well as superior selectivity and good stability. The present work provides a promising approach for the design of self-powered sensors which can be extended to detection of a wider range of environmental pollutants.

Screen Printing Preparation of Porous Cu Integrated Electrode as a High Sensitivity Enzyme-Free Glucose Sensor

Herein, a three-electrode coplanar integrated electrode was prepared by the screen-printing process. By designing a reasonable electrode structure, a scheme for the preparation of three-electrode coplanar Cu (OH)2/Ag/AgCl/C/Al2O3 integrated electrode by screen printing was proposed. The morphological structure of the material was investigated by SEM, EDS, and XRD characterization methods. Moreover, the availability of the electrodes was characterized macroscopically using an electrochemical workstation. As the results showed, the innovative sensor exhibited excellent detection performance for the catalytic oxidation of glucose. Electrode sensitivity was up to 14.376 mA mM−1 cm−2 in the glucose range of 1 μM–0.05 mM, while, excellent interference immunity and reproducibility were achieved.

Highly Sensitive and Indirect Electrochemical Sensing of Ascorbic Acid Based on the Catalytic Oxidation of Glucose on the Ni Nanoparticles-ionic Liquid Composite

Highly Sensitive and Indirect Electrochemical Sensing of Ascorbic Acid Based on the Catalytic Oxidation of Glucose on the Ni Nanoparticles-ionic Liquid Composite

Raspberry-like gold nanozyme-hybrid liposomes for hypoxia-enhanced biofilm eradication

Bacterial biofilm-associated infection has been one of the largest threats to global public health due to serious antibiotic resistance. It is urgently needed to develop new bactericides beyond antibiotics for biofilm eradication. Herein, we repurpose a hypoxia-activated anticancer drug tirapazamine (TPZ) to treat biofilm-associated infections based on the hypoxic biofilm microenvironment. A "hypoxia-enhanced" strategy is adopted to improve the therapeutic efficacy of TPZ by encapsulating TPZ into raspberry-like gold nanoparticle-hybrid liposomes (Lip@Au-TPZ). The gold nanoparticles on the hybrid liposomes can be used as nanozymes to catalyze glucose oxidation, which will consume oxygen to aggravate biofilm hypoxia. The aggravation of biofilm hypoxia is a critical step to metabolize TPZ into fatal oxidising radicals to induce DNA damage. The radical-induced damage of bacterial DNA by hypoxia-activated TPZ is fully investigated by multiple characterizations, including comet assay, nucleic acid gel electrophoresis, and bacterial gene transcriptome sequencing. Compared to liposome encapsulated TPZ (Lip-TPZ), Lip@Au-TPZ can lead to two orders of magnitude more reduction of bacterial cells due to the gold nanozyme-aggravated biofilm hypoxia. The biofilm eradication effect of Lip@Au-TPZ is further investigated in vivo by a mouse pneumonia model and a mouse wound healing model. The "hypoxia-enhanced" antibiofilm strategy by combining gold nanozymes and TPZ provides a new strategy for combating biofilm-associated infections. Data AvailabilityAll data supporting the findings of the current study are available in the article and its Supplemental Information. Additional relevant data are available from the corresponding author upon reasonable request.

Stepwise photoassisted decomposition of carbohydrates to H2

Stepwise photoassisted decomposition of carbohydrates to H2

Constructing a novel strategy for one-step colorimetric glucose biosensing based on Co-Nx sites on porous carbon as oxidase mimetics

Nanozyme-based sensing strategy for glucose generally involves a two-step H2O2-bridged cascade reaction. However, this two-step cascade reaction always shows a limited detection efficiency for glucose, which hardly makes simply operation, rapid detection, and sensitivity all promised. Herein, a novel strategy for one-step glucose detection was proposed based on the efficiently competitive relationship between glucose oxidase catalyzed glucose oxidation and oxidase nanozyme catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB) colorimetric oxidation. As a proof of concept, Co-Nx sites on porous carbon (Co-Nx/PC) derived from ZIF-67 was selected as the oxidase nanozyme with O2-sensitive nature, which can activate O2 to produce 1O2 for TMB colorimetric oxidation. In the presence of glucose, the competitive reaction of glucose oxidase catalyzed glucose oxidation occurs, which will obviously inhibit the Co-Nx/PC catalyzed TMB colorimetric oxidation to construct method for glucose detection. The usage of the same reactant O2 and the production of suppressive H2O2 cause a dual effect to this inhibition. In addition, the available pH range of Co-Nx/PC overlaps with that of glucose oxidase, promising a one pot reaction. The proposed method with Co-Nx/PC as oxidase nanozyme based on one-step competitive strategy for glucose detection possesses high detection efficiency with convenience (one-pot), rapidness (total reaction time of 10 min) and sensitivity (LOD of 0.31 μM) all promised, which is superior among the methods based on two-step one-pot or two-pot cascade strategy. The strategy can be successfully applied into the blood glucose detection in human serums with satisfactory results. Also, the strategy can be scalable to other oxidase nanozyme like Mn3O4.

A dual-nanozyme-loaded black phosphorus multifunctional therapeutic platform for combined photothermal/photodynamic/starvation cancer therapy.

The typical hypoxia of tumor microenvironments seriously affects the efficacy of starvation therapy (ST) and photodynamic therapy (PDT). Therefore, it is of great significance to prepare a multimodal combined therapy nanocomposite with the ability to relieve tumor hypoxia. It is an effective method to release oxygen into the tumor microenvironment using a nanoenzyme. In this work, two-dimensional black phosphorus nanosheets (BPNSs) were used as photothermal reagents and photosensitized agents due to the unique physical properties and also used as nano-carriers for the in situ reduction deposition of Au nanoparticles and fragmented MnO2 coatings. Finally, polyethylene glycol was introduced to obtain the nanocomposite BP@Au@MnO2-PEG (i.e., AMGP). In the tumor microenvironment, MnO2 can catalyze endogenous H2O2 to produce oxygen, improving the effect of ST and PDT. H2O2 generated via the catalytic oxidation of glucose by Au nanoparticles can continue to be used as the substrate catalyzed by MnO2. Meanwhile, black phosphorus nanosheets have excellent photothermal properties for photothermal treatment. The results of in vitro and in vivo experiments indicated that AMGP nanocomposites have good combined antitumor efficacies and biosafety.